scholarly journals High-yield production of secoisolariciresinol diglucoside from flaxseed hull by extraction with alcoholic ammonium hydroxide and chromatography on microporous resin

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Cong-Cong Zhuang ◽  
Chun-Rui Liu ◽  
Cheng-Bin Shan ◽  
Zheng Liu ◽  
Ling Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Ammonium Hydroxide ◽  
Extraction Time ◽  
High Yield ◽  
Extraction Temperature ◽  
Liquid Chromatography Mass Spectrometry ◽  
Secoisolariciresinol Diglucoside ◽  
One Pot ◽  
Yield Production ◽  
Level 3

AbstractThis study used alcoholic ammonium hydroxide to directly hydrolyze and extract secoisolariciresinol diglucoside (SDG) from flaxseed hull in a one pot reaction. The optimal extraction conditions, including the concentration of ammonium hydroxide, extraction time, and temperature, were examined in single factor experiments, followed by response surface methodology (RSM) with 3-level, 3-factor Box-Behnken experiments. As a result, the optimal extraction conditions were determined as follows: material-liquid ratio 1:20, percentage of reagent ammonium hydroxide (25–28% of NH3 in water) in ethanol 33.7% (pH = 12.9), extraction time 4.9 h, and extraction temperature 75.3 °C. Under these conditions, the yield of SDG, as measured by ultra-high-performance liquid chromatography-mass spectrometry, was 23.3 mg/g, consistent with the predicted content of SDG in flaxseed hull (23.0 mg/g). Further, 30.0 g of pulverized flaxseed hull was extracted under the optimal conditions, and the extract was subjected to a single run of macroporous resin chromatography to obtain 772.1 mg of a fraction with an SDG content exceeding 76.1%. Subsequent chromatography on Sephadex LH20, yielded 602.8 mg SDG of 98.0% purity, and the yield was 20.1 mg/g (2.0%) from flaxseed hulls. Thus, one-pot hydrolysis and extraction of SDG using alcoholic ammonium hydroxide is simple, and of high-yield. Graphical abstract

scholarly journals High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

2021 ◽  
Author(s):  
Benjamin Schmuck ◽  
Gabriele Greco ◽  
Andreas Barth ◽  
Nicola M. Pugno ◽  
Jan Johansson ◽  
...  
Keyword(s):  
High Performance ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

Efficient one-pot production of 1,2-propanediol and ethylene glycol from microalgae (Chlorococcum sp.) in water

2015 ◽  
Vol 17 (4) ◽  
pp. 2538-2544 ◽  
Author(s):  
G. Miao ◽  
C. C. Zhu ◽  
J. J. Wang ◽  
Z. C. Tan ◽  
L. Wang ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
High Yield ◽  
One Pot ◽  
Yield Production ◽  
High Yield Production

Direct one-pot valorization of aquatic microalgae for high yield production of alkanediols over an easily prepared Ni-based catalyst with excellent tolerance of nitrogen-containing components was demonstrated.

Maximized Extraction of Flavonoid Luteolin from V.negundo L. Leaves: Optimization Using Box-Behnken Design

2019 ◽  
Vol 15 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Lubna Abidin ◽  
Mohammad Mujeeb ◽  
Showkat R. Mir
Keyword(s):  
Process Parameters ◽  
High Performance ◽  
Optimization Technique ◽  
Optimization Method ◽  
Extraction Methods ◽  
Statistical Technique ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Extraction Field

Background: Luteolin is a flavonoid unveiling various therapeutic activities, found in Vitex negundo L. Thus, there is a need to present process parameters at which maximum amount of luteolin can be extracted from V. negundo L. leaves in “one-run”. Objective: Response surface methodology (RSM) was employed for optimizing the process parameters for the extraction of luteolin from V. negundo L. leaves. The study also compared the efficacy of various traditional and modern extraction methods for luteolin extraction. Methods: Extraction conditions (solvent to drug ratio, extraction temperature and extraction time) were optimized by RSM, Box-Behnken Design (BBD). Quantification of luteolin in various extracts was done through High Performance Liquid Chromatography (HPLC). Results: Hot solvent extraction by reflux technique stood out to be the best technique and methanol was found to be the most effective solvent for luteolin extraction.Through the use of BBD, the optimal conditions for luteolin extraction were established as: solvent to drug ratio- 17.7 mL/g, extraction temperature- 55.5°C and extraction time-2.04 hours. Under such conditions 7.32 %w/w of luteolin was yielded which was close to predicted value of 7.29 %w/w. Conclusion: Reflux technique stood out to be the best among all the studied modes of extraction and methanol proved to be the most effective solvent. Moreover, all the three variables significantly affected the luteolin extraction. Our study shows the applicability of a statistical technique, RSM in phytocompound extraction field. This makes the optimization technique cheap and less laborious than the traditional optimization method.

scholarly journals Optimization of Total Phenolic and Flavonoid Contents of Defatted Pitaya (Hylocereus polyrhizus) Seed Extract and Its Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 787 ◽  
Author(s):  
Siti Atikah Zulkifli ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Izuan Effendi Halmi
Keyword(s):  
High Performance ◽  
Ethanol Concentration ◽  
Antioxidant Properties ◽  
Extraction Process ◽  
Seed Extract ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

The present study was conducted to optimize extraction process for defatted pitaya seed extract (DPSE) adopting response surface methodology (RSM). A five-level central composite design was used to optimize total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis (3-ethylbenzothizoline-6-sulfonic acid (ABTS) activities. The independent variables included extraction time (30–60 min), extraction temperature (40–80 °C) and ethanol concentration (60%–80%). Results showed that the quadratic polynomial equations for all models were significant at (p < 0.05), with non-significant lack of fit at p > 0.05 and R2 of more than 0.90. The optimized extraction parameters were established as follows: extraction time of 45 min, extraction temperature of 70 °C and ethanol concentration of 80%. Under these conditions, the recovery of TPC, TFC, and antioxidant activity based on FRAP and ABTS were 128.58 ± 1.61 mg gallic acid equivalent (GAE)/g sample, 9.805 ± 0.69 mg quercetin equivalent (QE)/g sample, 1.23 ± 0.03 mM Fe2+/g sample, and 91.62% ± 0.15, respectively. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) analysis identified seven chemical compounds with flavonoids constituting major composition of the DPSE.

scholarly journals One-pot polyol synthesis of highly monodisperse short green silver nanorods

2016 ◽  
Vol 52 (73) ◽  
pp. 10960-10963 ◽  
Author(s):  
Javier Patarroyo ◽  
Aziz Genç ◽  
Jordi Arbiol ◽  
Neus G. Bastús ◽  
Victor Puntes
Keyword(s):  
Aspect Ratio ◽  
Tannic Acid ◽  
Polyol Method ◽  
Polyol Synthesis ◽  
High Yield ◽  
One Pot ◽  
Silver Nanorods ◽  
Low Aspect Ratio ◽  
Yield Production ◽  
High Yield Production

High yield production of green silver nanorods of a low aspect ratio via an optimized one-pot polyol method using tannic acid at substoichiometric concentrations.

A green strategy for obtaining anthraquinones from Rheum tanguticum by subcritical water

2020 ◽  
Vol 18 (1) ◽  
pp. 702-710
Author(s):  
Guoying Zhang ◽  
Xiaofeng Chi
Keyword(s):  
Flow Rate ◽  
High Speed ◽  
Subcritical Water ◽  
Extraction Time ◽  
High Yield ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Traditional Chinese Herbal Medicine ◽  
Anthraquinone Glycosides ◽  
Rheum Tanguticum

AbstractRheum tanguticum is a traditional Chinese herbal medicine, which contains abundant anthraquinones. In this study, anthraquinones were efficiently extracted from Rheum tanguticum by subcritical water extraction (SWE). The parameters of extraction time (33–67 min), temperature (100–200°C), and SW flow rate (1.4–4.6 mL/min) were optimized so as to achieve a high yield of the target product. A high yield of the total anthraquinones was achieved under the optimized SWE conditions of extraction time 54 min, extraction temperature 170°C, and the flow rate 2.0 mL/min. The comparison between the SWE and traditional extraction techniques implied that the SWE is an efficient and green alternative method for the extraction of anthraquinones. Four anthraquinone glycosides were purified from the SWE extract by high-speed counter-current chromatography and identified as emodin-1-O-β-D-glucoside, physcion-8-O-β-D-glucopyranoside, chrysophanol-1-O-β-D-glucoside, and chrysophanol-8-O-β-D-glucoside.

scholarly journals Optimasi Suhu dan Waktu Ekstraksi Akar Pasak Bumi (Eurycoma longifolia jack.) Menggunakan Metode RSM (response surface methodology) dengan Pelarut Etanol 70%

2021 ◽  
Vol 8 (1) ◽  
pp. 53
Author(s):  
Khoerul Anwar ◽  
Farida Istiqamah ◽  
Samsul Hadi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Preliminary Test ◽  
Extraction Time ◽  
Effect Of Temperature ◽  
High Yield ◽  
Extraction Temperature ◽  
Eurycoma Longifolia ◽  
Root Extraction ◽  
Eurycoma Longifolia Jack

Akar pasak bumi (Eurycoma longifolia Jack.) telah lama dimanfaatkan sebagai obat tradisional oleh masyarakat Indonesia sebagai aprodisiaka. Ekstraksi akar tumbuhan ini dilakukan dengan berbagai pelarut yang salah satunya menggunakan etanol 70%.  Pemilihan pelarut ini dilakukan untuk memperoleh kandungan zat berkhasiat semaksimal mungkin yang ditandai dengan rendemen yang tinggi. Penelitian ini bertujuan untuk menentukan suhu dan waktu ekstraksi optimum pada proses ekstraksi akar E. longifolia dengan pelarut etanol 70%. Metode OFAT (One Factor at The Time) digunakan pada uji pendahuluan dan metode RSM (Response Surface Methodology) digunakan pada desain eksperimen dengan bantuan software MINITAB 17. Penelitian dilakukan menggunakan 13 titik perlakuan dengan kombinasi suhu dan waktu yang berbeda. Hasil penelitian menunjukkan bahwa titik optimum rendemen ekstraksi akar E.longifolia sebesar 4,07% diperoleh pada suhu 51,8oC dan waktu 12,13 jam dengan nilai D (desirability) sebesar 0,92. Uji validasi model RSM menunjukkan keakuratan sebesar 97,76%. Model persamaan regresi yang menggambarkan pengaruh suhu dan waktu ekstraksi terhadap rendemen akar E. longifolia adalah Y = - 70,1 + 2,536X1 + 1,387X2 – 0,02389X12 – 0,0464X22 – 0,00500X1X2. Kata Kunci: Eurycoma longifolia Jack., Suhu dan Waktu Ekstraksi, Metode RSM, Etanol 70%The root of the pasak bumi (Eurycoma longifolia Jack.) has long been used as a traditional medicine by the Indonesian people as an aphrodisiac. Extraction of plant roots is carried out with various solvents, one of which uses ethanol 70%. The selection of this solvent was carried out to obtain the maximum possible active metabolite content which is characterized by high yield. This study aims to determine the optimum extraction temperature and time in the root extraction process of E. longifolia with 70% ethanol as solvent. The OFAT (One Factor at The Time) method was used in the preliminary test and the RSM (Response Surface Methodology) method was used in the experimental design with the help of MINITAB 17 software. The study was conducted using 13 treatment points with different combinations of temperature and time. The results showed that the optimum yield point of E. longifolia root extraction was 4.07% at a temperature of 51.8°C and extraction time of 12.13 hours with D (desirability) value of 0.92. The validation test of the RSM model shows an accuracy of 97.76%. The regression equation model that describes the effect of temperature and extraction time on the root yield of E. longifolia is Y = - 70.1 + 2.536X1 + 1.387X2 – 0.02389X12 – 0.0464X22 – 0.00500X1X2.

scholarly journals DEVELOPMENT OF METHODS FOR OBTAINING POLYSACCHARIDES FROM BIRCH LEAVES (BETULA PENDU-LA ROTH., BETULA PUBESCENS EHRH.)

2019 ◽  
pp. 23-31
Author(s):  
Kseniya Igorevna Rovkina ◽  
Sergey Vladimirovich Krivoshchekov ◽  
Artem Mikhaylovich Guriev ◽  
Mekhman Suleyman ogly Yusubov ◽  
Mikhail Valer'yevich Belousov
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Raw Materials ◽  
Gravimetric Method ◽  
Betula Pubescens ◽  
High Yield ◽  
Low Molecular Weight ◽  
Extraction Temperature ◽  
Extraction And Purification ◽  
Betula Pubescens Ehrh

The development of a complex technique for the extraction and purification of polysaccharides from birch leaves (Betula pendula Roth.,Betula pubescens Ehrh.) (PSfB) was the purpose of this study. The following characteristics were used as criteria for evaluating the effect of the parameters studied on the production of target substances: yield of PSfB (gravimetric method), protein content (spectrophotometric method), molecular weight distribution (high performance exclusion chromatography) and degree of purification from low molecular weight impurities (LMWI) (IR) spectroscopy).During the experiment, the optimal parameters were determined at various stages of obtaining the PSfB: degree of raw materials grinding – 1.2–3 mm, pH of the extractant (purified water with pH=7), the ratio of raw materials : extractant (1 : 20), extraction temperature (50 °C), temperature and degree of evaporation (50 °C; 4 times), the ratio of concentrate : ethanol (1 : 3) and the method of purification from the LMWI (ultrafiltration).The result of this work is an optimized method for obtaining PSfB, allowing to achieve high yield without loss of product quality (minimum allowable content of low molecular weight impurities with the highest content of high molecular weight fractions).This technique is the basis for the development of laboratory regulations for obtaining an active pharmaceutical substance based on polysaccharides from birch leaves.

scholarly journals EKSTRAKSI ACETOGENIN DARI DAUN DAN BIJI SIRSAK (Annona muricata L) DENGAN PELARUT ASETON

2016 ◽  
Vol 5 (2) ◽  
pp. 38-42
Author(s):  
Siswarni MZ ◽  
Nurhayani ◽  
Suci Damayanti Sinaga
Keyword(s):  
Extraction Time ◽  
High Yield ◽  
Second Step ◽  
Extraction Temperature ◽  
Annona Muricata ◽  
Ftir Analysis ◽  
Distillation Process ◽  
Yield Value ◽  
Seed Extraction ◽  
Materials Used

Annona muricata L contain bioactive compounds acetogenins. Acetogenins which was synthesized through reaction between polyketide derived-acetic acid with 35-39 carbon atom in fatty acid, is the secondary metabolite of Annonaceae plant. This research aims to determine the variable that influence in leaf and Annona muricata seed extraction so that high yield value can be obtained and to prove the presence of acetogenins compound qualitatively. The materials used are Annona muricata leaves, Annona muricata seeds and aceton. The changing variables in this research is the mass of the samples for Annona muricata leaves powder that are 15 g, 25 g, 35 g and Annona muricata seeds powder that are 10 g, 20 g, 30 g which the extraction time are 30 minutes, 40 minutes, 50 minutes and 60 minutes. This research divided into two steps. The first step is the extraction of Annona muricata leaves and Annona muricata seeds using soxhletation method with 250 mL acetone for Annona muricata leaves, 200 mL aceton for Annona muricata seeds and extraction temperature is ± 58 oC. The second step is purification of the extract using distillation process. In this research, the highest yield value extract of Annona muricata leaves obtained is 55,33% with 15g mass of sample and 60 minutes for the extraction time. The highest yield value extract of Annona muricata seeds obtained is 62 % with 10 g mass of sample and 40 minutes for the extraction time. FTIR analysis showed the presence of functional groups such as lactone, THF, hyrdroxyl and aliphatic chains which indicates acetogenins compound’s presence.

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