scholarly journals EKSTRAKSI ACETOGENIN DARI DAUN DAN BIJI SIRSAK (Annona muricata L) DENGAN PELARUT ASETON

2016 ◽  
Vol 5 (2) ◽  
pp. 38-42
Author(s):  
Siswarni MZ ◽  
Nurhayani ◽  
Suci Damayanti Sinaga
Keyword(s):  
Extraction Time ◽  
High Yield ◽  
Second Step ◽  
Extraction Temperature ◽  
Annona Muricata ◽  
Ftir Analysis ◽  
Distillation Process ◽  
Yield Value ◽  
Seed Extraction ◽  
Materials Used

Annona muricata L contain bioactive compounds acetogenins. Acetogenins which was synthesized through reaction between polyketide derived-acetic acid with 35-39 carbon atom in fatty acid, is the secondary metabolite of Annonaceae plant. This research aims to determine the variable that influence in leaf and Annona muricata seed extraction so that high yield value can be obtained and to prove the presence of acetogenins compound qualitatively. The materials used are Annona muricata leaves, Annona muricata seeds and aceton. The changing variables in this research is the mass of the samples for Annona muricata leaves powder that are 15 g, 25 g, 35 g and Annona muricata seeds powder that are 10 g, 20 g, 30 g which the extraction time are 30 minutes, 40 minutes, 50 minutes and 60 minutes. This research divided into two steps. The first step is the extraction of Annona muricata leaves and Annona muricata seeds using soxhletation method with 250 mL acetone for Annona muricata leaves, 200 mL aceton for Annona muricata seeds and extraction temperature is ± 58 oC. The second step is purification of the extract using distillation process. In this research, the highest yield value extract of Annona muricata leaves obtained is 55,33% with 15g mass of sample and 60 minutes for the extraction time. The highest yield value extract of Annona muricata seeds obtained is 62 % with 10 g mass of sample and 40 minutes for the extraction time. FTIR analysis showed the presence of functional groups such as lactone, THF, hyrdroxyl and aliphatic chains which indicates acetogenins compound’s presence.

scholarly journals EKSTRAKSI KUERSETIN DARI KULIT TERONG BELANDA (Solanum betaceum Cav.) MENGGUNAKAN PELARUT ETANOL DENGAN METODE MASERASI DAN SOKLETASI

2017 ◽  
Vol 6 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Siswarni MZ ◽  
Yusrina Ika Putri ◽  
Rizka Rinda P
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Extraction Time ◽  
High Yield ◽  
Aromatic Rings ◽  
Solvent Concentration ◽  
Independent Variables ◽  
Yield Value ◽  
Materials Used ◽  
Solanum Betaceum

The tamarillo peels (Solanum betaceum Cav.) contain flavonoid compounds, quercetin that can serve as antioxidant. This research aims to determine the best condition of operation in tamarillo peels extraction process to obtain the high yield value, and to prove the presence of antioxidant activity quantitatively. The materials used this research are tamarillo peels and ethanol. The independent variables in this research were the concentration of etanol which were 60%, 70%, 80% and 90% for maceration, and 70%, 80%, 90%, 99% for soxhletation. The extraction time are 3 days, 4 days, and 5 days for maceration and 3 hours, 4 hours, 5 hours for soxhletation. Extraction is done by maceration and soxhletation. The results of the qualitative analysis using FTIR represent the presence of functional groups such as keton, arylether, hydroxyl and aromatic rings which indicates quercetin compound’s presence. In quantitative analysis used Spectrofotometry UV-Vis, with the variation of 99% solvent concentration  and 5 hours of extraction time for soxhletation, the highest yield value obtained 0,0877 mg/g. The variation of 80% solvent concentration and 5 days of extraction time for maceration, the highest yield value obtained 0,0705 mg/g

scholarly journals High-yield production of secoisolariciresinol diglucoside from flaxseed hull by extraction with alcoholic ammonium hydroxide and chromatography on microporous resin

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Cong-Cong Zhuang ◽  
Chun-Rui Liu ◽  
Cheng-Bin Shan ◽  
Zheng Liu ◽  
Ling Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Ammonium Hydroxide ◽  
Extraction Time ◽  
High Yield ◽  
Extraction Temperature ◽  
Liquid Chromatography Mass Spectrometry ◽  
Secoisolariciresinol Diglucoside ◽  
One Pot ◽  
Yield Production ◽  
Level 3

AbstractThis study used alcoholic ammonium hydroxide to directly hydrolyze and extract secoisolariciresinol diglucoside (SDG) from flaxseed hull in a one pot reaction. The optimal extraction conditions, including the concentration of ammonium hydroxide, extraction time, and temperature, were examined in single factor experiments, followed by response surface methodology (RSM) with 3-level, 3-factor Box-Behnken experiments. As a result, the optimal extraction conditions were determined as follows: material-liquid ratio 1:20, percentage of reagent ammonium hydroxide (25–28% of NH3 in water) in ethanol 33.7% (pH = 12.9), extraction time 4.9 h, and extraction temperature 75.3 °C. Under these conditions, the yield of SDG, as measured by ultra-high-performance liquid chromatography-mass spectrometry, was 23.3 mg/g, consistent with the predicted content of SDG in flaxseed hull (23.0 mg/g). Further, 30.0 g of pulverized flaxseed hull was extracted under the optimal conditions, and the extract was subjected to a single run of macroporous resin chromatography to obtain 772.1 mg of a fraction with an SDG content exceeding 76.1%. Subsequent chromatography on Sephadex LH20, yielded 602.8 mg SDG of 98.0% purity, and the yield was 20.1 mg/g (2.0%) from flaxseed hulls. Thus, one-pot hydrolysis and extraction of SDG using alcoholic ammonium hydroxide is simple, and of high-yield. Graphical abstract

Optimization of Hydrodistillation of Rosemary Essential Oil

2020 ◽  
Vol 9 (3) ◽  
pp. 51-61
Author(s):  
Ouassila Larkeche ◽  
Ahmed Zermane ◽  
Abdeslam-Hassen Meniai ◽  
Souad Boubaira ◽  
Hayat Kouis ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plant Material ◽  
Extraction Process ◽  
Extraction Time ◽  
Operating Parameters ◽  
Distillation Process ◽  
Yield Value ◽  
Water Plant ◽  
Carbon Dioxide Extraction ◽  
Oil Components

Extraction of essential oil from Algerian rosemary leaves is carried out by means of a hydro-distillation process. The important operating parameters such as extraction time, condensation flow rate and water/solid plant material ratio, have been investigated. The highest yield value was 1.92% and was obtained for a water/plant ratio, a condensate flowrate, and extraction time of 20, 4.51ml/min and 120 min, respectively. The essential oil components have been identified using the GCMS analysis which show camphor as the major ingredient, followed by camphene, α-pinene and 1,8-cineole. The results have been compared with those obtained using a supercritical carbon dioxide extraction process, which has shown to be more much better quantitatively and qualitatively.

Optimization of Extraction of Bioactive Material-Polysaccharides from the Roots of Taraxacum mongolicum by Box-Behnken Experimental Design

2013 ◽  
Vol 749 ◽  
pp. 354-358
Author(s):  
Kai Chang ◽  
Guang Ren Sun ◽  
Da Di Yao
Keyword(s):  
Experimental Design ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Changbai Mountains ◽  
Extraction Temperature ◽  
Single Variable ◽  
Medical Systems ◽  
Extraction Parameters ◽  
Materials Used

Dandelion (Taraxacum spp) is an medical active materials used in many traditional and modern herbal medical systems, its polysaccharides have many bioactivities. Taraxacum mongolicum is one of the most widely consumed as Chinese herbal medicine and root tea in Changbai Mountains area, Northeast China. In this study, a procedure for the extraction of polysaccharides from the root of T mongolicum(TMRPS) was established. Based on single-variable tests experiment to determine the ranges of the extraction parameters (extraction temperature (X1), extraction time (X2), and ratio of solvent to raw material (X3)), response surface methodology (RSM) was used to optimize for these extraction parameters (X1, X2, X3) enhancing the forward extraction efficiency of TMRPS by a three-level three-variable Box-Behnken experimental design. The results showed that the ranges of the extraction parameters were determined as 60°C-80°C(X1),1h-3h (X2),20-40(v/m)(X3) by single-variable tests and the maximal yield (9.01%) of TMRPS was reached at extraction temperature 70.2°C, extraction time 1.79 h and ratio of solvent to raw material 28.0 (v):1 (m). Convenient for experiment, appropriate readjustment of these extraction parameters was made. Under these readjusted conditions, the mean extraction yield of TMRPS was 8.85%, which was near to theoretical calculation and indicates that these results were agree closely with model predictions.

A green strategy for obtaining anthraquinones from Rheum tanguticum by subcritical water

2020 ◽  
Vol 18 (1) ◽  
pp. 702-710
Author(s):  
Guoying Zhang ◽  
Xiaofeng Chi
Keyword(s):  
Flow Rate ◽  
High Speed ◽  
Subcritical Water ◽  
Extraction Time ◽  
High Yield ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Traditional Chinese Herbal Medicine ◽  
Anthraquinone Glycosides ◽  
Rheum Tanguticum

AbstractRheum tanguticum is a traditional Chinese herbal medicine, which contains abundant anthraquinones. In this study, anthraquinones were efficiently extracted from Rheum tanguticum by subcritical water extraction (SWE). The parameters of extraction time (33–67 min), temperature (100–200°C), and SW flow rate (1.4–4.6 mL/min) were optimized so as to achieve a high yield of the target product. A high yield of the total anthraquinones was achieved under the optimized SWE conditions of extraction time 54 min, extraction temperature 170°C, and the flow rate 2.0 mL/min. The comparison between the SWE and traditional extraction techniques implied that the SWE is an efficient and green alternative method for the extraction of anthraquinones. Four anthraquinone glycosides were purified from the SWE extract by high-speed counter-current chromatography and identified as emodin-1-O-β-D-glucoside, physcion-8-O-β-D-glucopyranoside, chrysophanol-1-O-β-D-glucoside, and chrysophanol-8-O-β-D-glucoside.

scholarly journals Optimasi Suhu dan Waktu Ekstraksi Akar Pasak Bumi (Eurycoma longifolia jack.) Menggunakan Metode RSM (response surface methodology) dengan Pelarut Etanol 70%

2021 ◽  
Vol 8 (1) ◽  
pp. 53
Author(s):  
Khoerul Anwar ◽  
Farida Istiqamah ◽  
Samsul Hadi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Preliminary Test ◽  
Extraction Time ◽  
Effect Of Temperature ◽  
High Yield ◽  
Extraction Temperature ◽  
Eurycoma Longifolia ◽  
Root Extraction ◽  
Eurycoma Longifolia Jack

Akar pasak bumi (Eurycoma longifolia Jack.) telah lama dimanfaatkan sebagai obat tradisional oleh masyarakat Indonesia sebagai aprodisiaka. Ekstraksi akar tumbuhan ini dilakukan dengan berbagai pelarut yang salah satunya menggunakan etanol 70%.  Pemilihan pelarut ini dilakukan untuk memperoleh kandungan zat berkhasiat semaksimal mungkin yang ditandai dengan rendemen yang tinggi. Penelitian ini bertujuan untuk menentukan suhu dan waktu ekstraksi optimum pada proses ekstraksi akar E. longifolia dengan pelarut etanol 70%. Metode OFAT (One Factor at The Time) digunakan pada uji pendahuluan dan metode RSM (Response Surface Methodology) digunakan pada desain eksperimen dengan bantuan software MINITAB 17. Penelitian dilakukan menggunakan 13 titik perlakuan dengan kombinasi suhu dan waktu yang berbeda. Hasil penelitian menunjukkan bahwa titik optimum rendemen ekstraksi akar E.longifolia sebesar 4,07% diperoleh pada suhu 51,8oC dan waktu 12,13 jam dengan nilai D (desirability) sebesar 0,92. Uji validasi model RSM menunjukkan keakuratan sebesar 97,76%. Model persamaan regresi yang menggambarkan pengaruh suhu dan waktu ekstraksi terhadap rendemen akar E. longifolia adalah Y = - 70,1 + 2,536X1 + 1,387X2 – 0,02389X12 – 0,0464X22 – 0,00500X1X2. Kata Kunci: Eurycoma longifolia Jack., Suhu dan Waktu Ekstraksi, Metode RSM, Etanol 70%The root of the pasak bumi (Eurycoma longifolia Jack.) has long been used as a traditional medicine by the Indonesian people as an aphrodisiac. Extraction of plant roots is carried out with various solvents, one of which uses ethanol 70%. The selection of this solvent was carried out to obtain the maximum possible active metabolite content which is characterized by high yield. This study aims to determine the optimum extraction temperature and time in the root extraction process of E. longifolia with 70% ethanol as solvent. The OFAT (One Factor at The Time) method was used in the preliminary test and the RSM (Response Surface Methodology) method was used in the experimental design with the help of MINITAB 17 software. The study was conducted using 13 treatment points with different combinations of temperature and time. The results showed that the optimum yield point of E. longifolia root extraction was 4.07% at a temperature of 51.8°C and extraction time of 12.13 hours with D (desirability) value of 0.92. The validation test of the RSM model shows an accuracy of 97.76%. The regression equation model that describes the effect of temperature and extraction time on the root yield of E. longifolia is Y = - 70.1 + 2.536X1 + 1.387X2 – 0.02389X12 – 0.0464X22 – 0.00500X1X2.

scholarly journals Skin-Whitening and Anti-Wrinkle Effects of Bioactive Compounds Isolated from Peanut Shell Using Ultrasound-Assisted Extraction

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1231
Author(s):  
Da Hye Gam ◽  
Ji Woo Hong ◽  
Jun Hee Kim ◽  
Jin Woo Kim
Keyword(s):  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Peanut Shell ◽  
Ultrasound Assisted Extraction ◽  
Skin Whitening ◽  
Activity Inhibition ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Experimental Values

Response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) conditions for simultaneous optimization of dependent variables, including DPPH radical scavenging activity (RSA), tyrosinase activity inhibition (TAI), and collagenase activity inhibition (CAI) of peanut shell extracts. The effects of the main variables including extraction time (5.0~55.0 min, X1), extraction temperature (26.0~94.0 °C, X2), and ethanol concentration (0.0%~99.5%, X3) were optimized. Based on experimental values from each condition, quadratic regression models were derived for the prediction of optimum conditions. The coefficient of determination (R2) of the independent variable was in the range of 0.89~0.96, which demonstrates that the regression model is suitable for the prediction. In predicting optimal UAE conditions based on the superimposing method, extraction time of 31.2 min, extraction temperature of 36.6 °C, and ethanol concentration of 93.2% were identified. Under these conditions, RSA of 74.9%, TAI of 50.6%, and CAI of 86.8% were predicted, showing good agreement with the experimental values. A reverse transcription polymerase chain reaction showed that peanut shell extract decreased mRNA levels of tyrosinase-related protein-1 and matrix metalloproteinase-3 genes in B16-F0 cell. Therefore, we identified the skin-whitening and anti-wrinkle effects of peanut shell extracts at protein as well as gene expression levels, and the results show that peanut shell is an effective cosmetic material for skin-whitening and anti-wrinkle effects. Based on this study, peanut shell, which was considered a byproduct, can be used for the development of healthy foods, medicines, and cosmetics.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Microwave-Assisted Extraction of Chlorophyll from Filter Mud of Sugercane Mill and Component Analysis

2012 ◽  
Vol 518-523 ◽  
pp. 430-435 ◽  
Author(s):  
Hai Rong Guo ◽  
Shao Ying Ma ◽  
Xiao Fei Wang ◽  
Er Fang Ren ◽  
Yuan Yuan Li
Keyword(s):  
Irradiation Time ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction ◽  
Filter Mud ◽  
Strong Absorption Peak

Microwave-assisted extraction (MAE) was used to extract chlorophylls from filter mud. Ethanol was used as the solvent. The optimal conditions for the MAE of chlorophylls were concluded from the study as the irradiation time, 50 s, the ratio of liquid to solid, 8:1 (mL/g), the extraction temperature, 40 °C, and the extraction time, 60 min. Compared with conventional extraction, the MAE of chlorophylls from the filter mud was more effective. The extraction time for MAE was 60 min with 0.277 mg/g chlorophyll yield, while conventional extraction needed 240 min with only about 0.259 mg/g chlorophyll yield. The Ultraviolet Absorption Spectra of the extracted chlorophylls showed that there was a strong absorption peak at about 663 nm. C=N, Mg-N and C-N was not seen existed from the infrared spectroscopy probably because that the mixed extracts were not purified and the chlorophyll content was less.

Optimization of the Ultrasonic Extraction of Gypenoside III from Gynostemma pentaphyllum by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1866-1870
Author(s):  
Xiao Dan Tang ◽  
Hai Yang Hang ◽  
Shao Yan Wang ◽  
Jing Xiang Cong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Gynostemma Pentaphyllum ◽  
Bioactive Component ◽  
Yield Value ◽  
Ultrasonic Time

Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.

Sign in / Sign up

Export Citation Format

Share Document