Maximized Extraction of Flavonoid Luteolin from V.negundo L. Leaves: Optimization Using Box-Behnken Design

2019 ◽  
Vol 15 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Lubna Abidin ◽  
Mohammad Mujeeb ◽  
Showkat R. Mir
Keyword(s):  
Process Parameters ◽  
High Performance ◽  
Optimization Technique ◽  
Optimization Method ◽  
Extraction Methods ◽  
Statistical Technique ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Extraction Field

Background: Luteolin is a flavonoid unveiling various therapeutic activities, found in Vitex negundo L. Thus, there is a need to present process parameters at which maximum amount of luteolin can be extracted from V. negundo L. leaves in “one-run”. Objective: Response surface methodology (RSM) was employed for optimizing the process parameters for the extraction of luteolin from V. negundo L. leaves. The study also compared the efficacy of various traditional and modern extraction methods for luteolin extraction. Methods: Extraction conditions (solvent to drug ratio, extraction temperature and extraction time) were optimized by RSM, Box-Behnken Design (BBD). Quantification of luteolin in various extracts was done through High Performance Liquid Chromatography (HPLC). Results: Hot solvent extraction by reflux technique stood out to be the best technique and methanol was found to be the most effective solvent for luteolin extraction.Through the use of BBD, the optimal conditions for luteolin extraction were established as: solvent to drug ratio- 17.7 mL/g, extraction temperature- 55.5°C and extraction time-2.04 hours. Under such conditions 7.32 %w/w of luteolin was yielded which was close to predicted value of 7.29 %w/w. Conclusion: Reflux technique stood out to be the best among all the studied modes of extraction and methanol proved to be the most effective solvent. Moreover, all the three variables significantly affected the luteolin extraction. Our study shows the applicability of a statistical technique, RSM in phytocompound extraction field. This makes the optimization technique cheap and less laborious than the traditional optimization method.

Response Surface Methodology Optimization for Extraction of Natural Anthocyanins from Vietnamese Carissa сarandas L. Fruit

2019 ◽  
Vol 814 ◽  
pp. 475-480
Author(s):  
Tri Nhut Pham ◽  
Thi Ha Xuan Huynh ◽  
Bui Phuc Tran ◽  
Thien Hien Tran ◽  
Phu Thuong Nhan Nguyen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Fruits And Vegetables ◽  
Extraction Methods ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Naturally Occurring ◽  
Carissa Carandas

Anthocyanins are naturally occurring compounds that are responsible for a wide variety of colors in many plants, fruits and vegetables. In this study, the extraction of natural anthocyanins from Vietnamese Carissa carandas L. beverage was optimized using response surface methodology (RSM). We applied a Box–Behnken design consisting of three levels and three factors. Examined factors are extraction temperature (ranging from 40 to 60°C), liquid to solid ratio (ranging from 2:1 to 4:1), extraction time (ranging from 30 to 60 min). Using 60% ethanol as solvent for the process, we determined the maximum yields of anthocyanin was 273.786 mg/L. This yield corresponds to extraction conditions of 3:1 (v/w) liquid to solid ratio, temperature of 48.10 °C with a 44.08 min extraction time. The experimental results also fit well with the proposed response model of anthocyanin yield (R2 = 0.9992). Therefore, this study suggested optimization of different extraction methods for the defatted fruit parts.

scholarly journals Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark ofZiziphus jujubaeL.

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Kashyap Kumar Dubey ◽  
Nitika Goel
Keyword(s):  
Process Parameters ◽  
Betulinic Acid ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Optimum Parameters ◽  
Assisted Extraction ◽  
Recovery Percentage ◽  
Percentage Extraction ◽  
Rsm Model

Present work investigated an apposite and efficient method for extraction of betulinic acid (BA) from the bark ofZiziphus jujubae. Various extraction methods like stirring extraction, soxhlet extraction, ultrasonic extraction, and microwave assisted extraction (MAE) were evaluated for increasing recovery percentage of BA. From the raffinate so obtained, BA was isolated. Thin layer chromatography (TLC) was used to analyze the extract and high performance liquid chromatography (HPLC) for quantification. The results revealed that the percentage extraction of BA fromZ. jujubaeby MAE was more proficient. As recovery percentage of BA by MAE technique turned out to be maximum, by using response surface methodology (RSM), three process parameters (pH, temperature, and time) were optimized by MAE and it was observed that the optimum parameters (pH 6.5, temp. 70.23°C, and time 3.5 min) gave the maximum recovery of BA (0.44% w/w). To validate the RSM model, experiments were performed and the highest recovery of BA was found to be 0.4% w/w which is ±0.04% to the predicted value. Henceforth the extraction efficiency and the substantial saving of time by MAE was more capable than the other extraction techniques.

Optimization of Ultrasonic Extraction of Phenolics from Litchi Seed by Response Surface Methodology

2012 ◽  
Vol 610-613 ◽  
pp. 3387-3393
Author(s):  
Xiao Bing Huang ◽  
Li Jing Lin ◽  
Ji Hua Li ◽  
Xu Ran ◽  
Yong Fu Tang
Keyword(s):  
Response Surface Methodology ◽  
Regression Model ◽  
Response Surface ◽  
Maximum Yield ◽  
Methanol Concentration ◽  
Ultrasonic Extraction ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Quadratic Regression ◽  
Box Behnken Design

Optimization conditions for ultrasonic extraction of phenolics from litchi seed were studied using response surface methodology. A Box–Behnken design (BBD) was applied to determine the effects of extraction temperature, methanol concentration and extraction time on yield of phenolics. Then a quadratic regression model was developed and found to be statistically significant by examining its adequacy. According to the model, the maximum yield of phenolics was obtained at the theoretical extraction conditions described as follows: extraction temperature 90°C, methanol concentration 59% and extraction time 70 min. Under this condition, the experimental value was 5.48 ± 0.03% (n = 3) with gallic acid as the equivalent, which agreed with the predicted value (5.52%) closely.

scholarly journals Determining the Optimum Conditions for Extracting Carotenoids from Syrian Apricot Fruits

2021 ◽  
pp. 11-20
Author(s):  
Wahbi Kalook ◽  
Adib Faleh ◽  
Amir Al-Haj Sakur ◽  
Wassim Abdelwahed
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Extraction Process ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Optimum Temperature ◽  
Optimum Conditions ◽  
Total Carotenoids ◽  
Β Carotene

The aim of this research is to extract carotenoids from apricot fruits using a food solvent (ethanol) and a non-food solvent (propanol). In addition, it aims to study the effect of different extraction conditions, i.e., the extraction temperature (20-40-60)°C and the extraction times (4-8-12) hours, on the carotenoids yield in order to improve and develop extraction methods. The extracted carotenoids were determined using high-performance liquid chromatography (HPLC), and the studied carotenoids are α-carotene and β-carotene. It was found that β-carotene constitutes about 80% of the total carotenoids in apricots. The results indicated that the extraction yield using ethanol was low compared to the extraction yield using propanol. Propanol is the most suitable solvent for carotenoids extraction in comparison with ethanol. The temperature also had a significant effect on the extraction yield; at 20°C the extraction yield was very low, and 60°C was the optimum temperature for the extraction of carotene. The extraction yield significantly increased with time in the first hours of extraction, and there was no significant effect from increasing the extraction time for a period of 6-8 hours. Moreover, the pretreatment of fruits by freezing accelerated the extraction process and increased the extraction yield. The optimum conditions for extracting carotenoids were determined in the conditions of food extraction; the optimum conditions for extracting carotenoids are: extraction at 60°C for three hours with pretreatment by freezing as the extraction rate reached up to 6.36 mg/100 g using ethanol as a food solvent.

scholarly journals High-yield production of secoisolariciresinol diglucoside from flaxseed hull by extraction with alcoholic ammonium hydroxide and chromatography on microporous resin

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Cong-Cong Zhuang ◽  
Chun-Rui Liu ◽  
Cheng-Bin Shan ◽  
Zheng Liu ◽  
Ling Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Ammonium Hydroxide ◽  
Extraction Time ◽  
High Yield ◽  
Extraction Temperature ◽  
Liquid Chromatography Mass Spectrometry ◽  
Secoisolariciresinol Diglucoside ◽  
One Pot ◽  
Yield Production ◽  
Level 3

AbstractThis study used alcoholic ammonium hydroxide to directly hydrolyze and extract secoisolariciresinol diglucoside (SDG) from flaxseed hull in a one pot reaction. The optimal extraction conditions, including the concentration of ammonium hydroxide, extraction time, and temperature, were examined in single factor experiments, followed by response surface methodology (RSM) with 3-level, 3-factor Box-Behnken experiments. As a result, the optimal extraction conditions were determined as follows: material-liquid ratio 1:20, percentage of reagent ammonium hydroxide (25–28% of NH3 in water) in ethanol 33.7% (pH = 12.9), extraction time 4.9 h, and extraction temperature 75.3 °C. Under these conditions, the yield of SDG, as measured by ultra-high-performance liquid chromatography-mass spectrometry, was 23.3 mg/g, consistent with the predicted content of SDG in flaxseed hull (23.0 mg/g). Further, 30.0 g of pulverized flaxseed hull was extracted under the optimal conditions, and the extract was subjected to a single run of macroporous resin chromatography to obtain 772.1 mg of a fraction with an SDG content exceeding 76.1%. Subsequent chromatography on Sephadex LH20, yielded 602.8 mg SDG of 98.0% purity, and the yield was 20.1 mg/g (2.0%) from flaxseed hulls. Thus, one-pot hydrolysis and extraction of SDG using alcoholic ammonium hydroxide is simple, and of high-yield. Graphical abstract

scholarly journals Simultaneous degradation of nitrogenous heterocyclic compounds by catalytic wet-peroxidation process using box-behnken design

2019 ◽  
Vol 25 (4) ◽  
pp. 488-497
Author(s):  
Vijayalakshmi Gosu ◽  
Shivali Arora ◽  
Verraboina Subbaramaiah
Keyword(s):  
Hydrogen Peroxide ◽  
Process Parameters ◽  
Heterocyclic Compounds ◽  
Optimization Technique ◽  
Process Condition ◽  
Quadratic Model ◽  
Box Behnken Design ◽  
Initial Concentration ◽  
Toc Removal ◽  
Initial Ph

The present study investigates the feasibility of nitrogenous heterocyclic compounds (NHCs) (Pyridine-Quinoline) degradation by catalytic wet peroxidation (CWPO) in the presence of nanoscale zerovalent iron supported on granular activated carbon (nFe<sup>0</sup>/GAC) using statistical optimization technique. Response surface methodology (RSM) in combination with Box-Behnken design (BBD) was used to optimize the process parameters of CWPO process such as initial pH, catalyst dose, hydrogen peroxide dose, initial concentration of pyridine (Py) and quinolone (Qn) were chosen as the main variables, and total organic carbon (TOC) removal and total Fe leaching were selected as the investigated response. The optimization of process parameters by desirability function showed the ~85% of TOC removal with process condition of initial solution pH 3.5, catalyst dose of 0.55 g/L, hydrogen peroxide concentration of 0.34 mmol, initial concentration of Py 200 mg/L and initial concentration of Qn 200 mg/L. Further, for TOC removal the analysis of variance results of the RSM revealed that all parameter i.e. initial pH, catalyst dose, hydrogen peroxide dose, initial concentration of Py and initial concentration of Qn were highly significant according to the <i>p</i> values (<i>p</i> < 0.05). The quadratic model was found to be the best fit for experimental data. The present study revealed that BBD was reliable and effective for the determination of the optimum conditions for CWPO of NHCs (Py-Qn).

scholarly journals Optimization of Total Phenolic and Flavonoid Contents of Defatted Pitaya (Hylocereus polyrhizus) Seed Extract and Its Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 787 ◽  
Author(s):  
Siti Atikah Zulkifli ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Izuan Effendi Halmi
Keyword(s):  
High Performance ◽  
Ethanol Concentration ◽  
Antioxidant Properties ◽  
Extraction Process ◽  
Seed Extract ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

The present study was conducted to optimize extraction process for defatted pitaya seed extract (DPSE) adopting response surface methodology (RSM). A five-level central composite design was used to optimize total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis (3-ethylbenzothizoline-6-sulfonic acid (ABTS) activities. The independent variables included extraction time (30–60 min), extraction temperature (40–80 °C) and ethanol concentration (60%–80%). Results showed that the quadratic polynomial equations for all models were significant at (p < 0.05), with non-significant lack of fit at p > 0.05 and R2 of more than 0.90. The optimized extraction parameters were established as follows: extraction time of 45 min, extraction temperature of 70 °C and ethanol concentration of 80%. Under these conditions, the recovery of TPC, TFC, and antioxidant activity based on FRAP and ABTS were 128.58 ± 1.61 mg gallic acid equivalent (GAE)/g sample, 9.805 ± 0.69 mg quercetin equivalent (QE)/g sample, 1.23 ± 0.03 mM Fe2+/g sample, and 91.62% ± 0.15, respectively. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) analysis identified seven chemical compounds with flavonoids constituting major composition of the DPSE.

scholarly journals Optimization of Gentisides Extraction fromGentiana rigescensFranch. ex Hemsl. by Response Surface Methodology

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Bowen Chu ◽  
Yao Shi ◽  
Zhimin Li ◽  
Hao Tian ◽  
Wanyi Li ◽  
...  
Keyword(s):  
Response Surface ◽  
Raw Material ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Extraction Processes ◽  
Optimized Conditions ◽  
Therapeutic Substance ◽  
Gentiana Rigescens ◽  
Univariate Test

Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome ofGentiana rigescensFranch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.61±0.61).

Comparison of Microwave Method with Traditional Method to Extract Tea Polyphenols

2014 ◽  
Vol 933 ◽  
pp. 183-186
Author(s):  
Yan Xia Han ◽  
Gong Nan Wang ◽  
Zhao Zhong Shi ◽  
Fang Wang
Keyword(s):  
Traditional Method ◽  
Optimization Method ◽  
Extraction Process ◽  
Extraction Time ◽  
Primary Study ◽  
Microwave Method ◽  
Extraction Temperature ◽  
Microwave Extraction ◽  
Orthogonal Optimization ◽  
Extraction Liquid

In this research, the primary study in extracting technologies of Tea polyphends was finished. On the basis of the water lixiviation by orthogonal optimization method, extraction process of TP by microwave extraction was optimized. The best combined condition of the water lixiviation as follows: liquid-lolid ratio 25:1, extraction temperature 95°C , extraction time 20min,Under this conditions, the yield of TP is 13.993 %.And the best combined condition by microwave extraction: liquid-lolid ratio 35:1, microwave power 750 W, extraction time 18min, and the yield of TP is 20.3023 % through duplicated experiment. The results showed that compare with water lixiviation, the yield of TP was enhanced by 6.3093 % by microwave method. Meanwhile, the extraction time by microwave method was reduced greatly.

scholarly journals Application of Box–Behnken Design to Investigate the Effect of Process Parameters on the Microparticle Production of Ethenzamide through the Rapid Expansion of the Supercritical Solutions Process

Pharmaceutics ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 42 ◽  
Author(s):  
Yung-Tai Hsu ◽  
Chie-Shaan Su
Keyword(s):  
Thermal Behavior ◽  
Process Parameters ◽  
Crystal Form ◽  
Rapid Expansion ◽  
Crystal Habit ◽  
Extraction Temperature ◽  
Box Behnken Design ◽  
Mean Size ◽  
Supercritical Solutions ◽  
Expansion Temperature

In this study, the rapid expansion of the supercritical solutions (RESS) process was used to produce microparticles of a commonly used anti-inflammatory drug, ethenzamide. The effects of process parameters in RESS including the extraction temperature, pre-expansion temperature, and post-expansion temperature were investigated using the Box–Behnken design. According to the results of the analysis of variance (ANOVA), the effect of pre-expansion temperature is the most significant parameter on the mean size of RESS-produced ethenzamide. A higher pre-expansion temperature benefits the production of smaller crystals. In addition, a quadratic effect of the post-expansion temperature was also identified. Through RESS, ethenzamide microparticles with a mean size of 1.6 μm were successfully produced. The solid-state properties including the crystal habit, crystal form, thermal behavior, and spectrometric property were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectrometer (FTIR), differential scanning calorimeter (DSC), and powder X-ray diffraction (PXRD). These analytical results show that the rod-like crystals were generated through RESS, and the crystal form, thermal behavior, and spectrometric property of RESS-produced crystals are consistent with the unprocessed ethenzamide.

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