scholarly journals Bacterial isolates and their antibiotic sensitivity pattern of surgical site infections among the surgical ward patients of Asella Referral and Teaching Hospital

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Teshome Gebissa ◽  
Bonge Bude ◽  
Mohd Yasir ◽  
Shimelis Mekit ◽  
K. M. Noorulla
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Surgical Site Infection ◽  
Teaching Hospital ◽  
Antimicrobial Agents ◽  
Bacterial Pathogens ◽  
Surgical Site Infections ◽  
Site Infection ◽  
Bacterial Isolates

Abstract Background Surgical site infection (SSI) is the most typical problem for patients who undergo operative procedures. It remains a typical and widespread problem causing morbidity and mortality, partly related to a rise in infections due to antimicrobial-resistant bacterial pathogens. The study was purposed to evaluate the bacterial isolates and their drug susceptibility patterns in patients with postoperative surgical site infection. Results The rate of postoperative surgical site infections was studied at Asella Referral and Teaching Hospital and found to be 23.3%. One hundred fifty specimens of pus and surface swabs were collected from the surgical site-infected patients over the period of March 2016 to May 2017, and from that, a total of 147 bacterial pathogens were recovered. The predominant organisms associated with postoperative surgical site infections were Klebsiella species 38 (26%, n=147), Escherichia coli 31 (21%, n=147), Staphylococcus aureus 25 (17%, n=147), and Pseudomonas aeruginosa 18 (12%, n=147). Conclusion Higher number of bacterial isolates were recovered. The predominant isolates were Klebsiella species, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The majority of gram-negative bacterial species which were isolated were found to be resistant to the commonly prescribed antimicrobial agents in the study setting. Thus, to achieve effective therapy for wound infections and to reduce/stop the appearance of multidrug-resistant (MDR) pathogens, continuous monitoring is essential with the fair use of antimicrobial agents.

scholarly journals Magnitude of Multidrug Resistance among Bacterial Isolates from Surgical Site Infections in Two National Referral Hospitals in Asmara, Eritrea

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Eyob Yohannes Garoy ◽  
Yacob Berhane Gebreab ◽  
Oliver Okoth Achila ◽  
Nobiel Tecklebrhan ◽  
Hermon Michael Tsegai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Surgical Site Infections ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Referral Hospitals

Background. The World Health Organization has emphasized the importance of understanding the epidemiology of MDR organisms from a local standpoint. Here, we report on a spectrum of bacteria associated with surgical site infections in two referral hospitals in Eritrea and the associated antibiotic susceptibility patterns. Methods. This survey was conducted between February and May 2017. A total of 83 patients receiving treatment for various surgical conditions were included. Swabs from infected surgical sites were collected using Levine technique and processed using standard microbiological procedures. In vitro antimicrobial susceptibility testing was performed on Mueller–Hinton Agar by the Kirby-Bauer disk diffusion method following Clinical and Laboratory Standards Institute guidelines. The data were analyzed using SPSS version 20. Results. A total of 116 isolates were recovered from 83 patients. In total, 67 (58%) and 49 (42%) of the isolates were Gram-positive and Gram-negative bacteria, respectively. The most common isolates included Citrobacter spp., Klebsiella spp., Escherichia coli, Proteus spp., Pseudomonas aeruginosa, Salmonella spp., Enterobacter spp., and Acinetobacter spp. In contrast, Staphylococcus aureus, CONS, and Streptococcus viridians were the predominant Gram-positive isolates. All the Staphylococcus aureus isolates were resistant to penicillin. MRSA phenotype was observed in 70% of the isolates. Vancomycin, clindamycin, and erythromycin resistance were observed in 60%, 25%, and 25% of the isolates, respectively. Furthermore, a high proportion (91%) of the Gram-negative bacteria were resistant to ampicillin and 100% of the Pseudomonas aeruginosa and Escherichia coli isolates were resistant to >5 of the tested antibiotics. The two Acinetobacter isolates were resistant to >7 antimicrobial agents. We also noted that 4 (60%) of the Klebsiella isolates were resistant to >5 antimicrobial agents. Possible pan-drug-resistant (PDR) strains were also isolated. Conclusion. Due to the high frequency of MDR isolates reported in this study, the development and implementation of suitable infection control policies and guidelines is imperative.

scholarly journals MRSA – ‘Bug-Bear’ of a Surgical Practice: Reducing the Incidence of MRSA Surgical Site Infections

2006 ◽  
Vol 88 (2) ◽  
pp. 222-223 ◽  
Author(s):  
Andrea Guyot ◽  
Graham Layer
Keyword(s):  
Staphylococcus Aureus ◽  
Surgical Site Infection ◽  
Orthopaedic Surgery ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Surgical Site Infections ◽  
Site Infection ◽  
Infection Rates ◽  
Methicillin Resistant ◽  
Surgical Practice ◽  
Department Of Health

Adverse publicity (the ‘superbug') has demonstrated that the problem of MRSA (methicillin-resistant Staphylococcus aureus) is prevalent in many of the country's most prestigious hospitals. The results of the mandatory UK Department of Health (DH) surveillance for early surgical site infections in orthopaedic surgery (SSIS) have been published recently for the period April 2004 to March 2005 when 41,242 operations were studied (< http://www.dh.gov.uk/PublicationsAndStatistics/Publications/PublicationsStatistic > 28 October 2005). Infection rates were generally and gratifyingly low but 48% of surgical site infections were caused by Staph. aureus and of those 68% were MRSA. The following article will discuss the aetiology and prevention of MRSA surgical site infection.

scholarly journals Microorganisms Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
O. G. Palmer ◽  
A. K. Onifade
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Bacterial Isolates ◽  
Environmental Surfaces ◽  
Ondo State

Nosocomial infection is a rising problem in developing countries. Microorganisms Isolated from three private and public hospital environmental surfaces in Akure Metropolis, Ondo State, Nigeria were investigated in this study. Bacterial and fungal organisms were isolated and compared among the three hospitals. The study revealed that bacteria were the most predominant microorganisms found in the hospital environmental surfaces than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans.  Staphylococcus aureus was found to be predominant bacteria but Aspergillus funmigatus was the predominant fungi. The result showed that the microbial loads of the public hospitals were higher than that of the private hospitals. The bacteria load of the male ward was found to be higher than that of the female ward while the fungal loads of each of the hospital environmental surfaces of female were higher than that of the male. The study revealed that bacteria were the most predominant microorganisms found in the hospital environment than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans.  Staphylococcus aureus was found to be predominant bacteria. All the hospital environmental surfaces were contaminated with one or more microorganisms in the course of the research.

Comparative Study of the Antimicrobial Effect of Different Antibiotics Mixed with CuO, MgO and ZnO Nanoparticles in Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli cultures

2014 ◽  
Vol 1675 ◽  
pp. 59-64
Author(s):  
Raúl Alenó ◽  
Anthony López Collazo ◽  
Eulalia Medina ◽  
Lourdes Díaz Figueroa ◽  
José I. Ramírez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cell Death ◽  
Optical Properties ◽  
Antibacterial Agents ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Antibacterial Effects ◽  
New Methods

ABSTRACTDue to the rapid advance of the emergence of resistant microorganisms to different antibiotics, there is a need to create new antimicrobial agents. It is possible that Nanotechnology has a great impact in this area since the nanoparticles can improve the antimicrobial effect of the antibiotics. In this study we used three different metal oxides nanoparticles, the MgO, ZnO and CuO. These nanoparticles were selected because their interactions leading to cell death and their optical properties. The aim of this study is to develop new methods that are more effective against resistance bacteria, developing antibacterial agents using different nanoparticles against Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 10145), and Staphylococcus aureus (ATCC BAA-1026). This study was conducted to evaluate the antibacterial effects of a combination of nanoparticles together with different concentrations of three antibiotics, Gentamicin, Cephalexin and Co-Trimoxazole. The results showed that some nanoparticles are effective to inhibit growth in these microorganisms by increasing the effectiveness of the antibiotic. Therefore, the present study indicates that the combination of the nanoparticles with antibiotics may be applicable as a new antimicrobial agent.

scholarly journals Synthesis and biological evaluation of some new 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as antimicrobial agents

2022 ◽  
Vol 11 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Iryna Myrko ◽  
Taras Chaban ◽  
Vasyl Matiychuk
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Synthesis And Biological Evaluation

A series of some new pyrazole-substituted 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines was synthesized in this study. The structures of target substances were confirmed by using 1H and 13С NMR spectroscopy, mass spectrometry and elemental analysis. The synthesized compounds have been evaluated for antimicrobial activity against five bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus) and two fungal strains (Candida albicans and Cryptococcus neoformans). The antimicrobial screening studies of synthesized substances established that 2 of 12 compounds show pronounced antibacterial activity against the strain Staphylococcus aureus.

scholarly journals In-vitro antibacterial activity of sequential crude extracts from Datura stramonium seeds

2016 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Powar Priyatama V ◽  
Powar Trupti A
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Datura Stramonium ◽  
Seed Extract ◽  
Zone Of Inhibition

The growing phenomenon of antibiotic resistance, particularly to pathogenic microorganisms, in current medicine, has directed the concern of scientists for finding novel antimicrobial agents from plant origin with negligible side effect. The present study was aimed to phytochemical investigation and antimicrobial activity of seed extract of Datura stramonium in sequentially with different organic solvents. For this, antimicrobial properties were tested against bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Bacillus subtilis by cup plate method. Among the tested bacterial Klebsiella pneumoniae was the most inhibited majorly with the chloroform extract. Datura stramonium chloroform seed extract produced maximum zone of inhibition 26 mm against Klebsiella pneumoniae and 12 mm against Bacillus subtilis and 13 mm against Escherichia coli. Datura Stramonium methanol seed extract produced maximum zone of inhibition 27 mm against Pseudomonas aeruginosa and 15 mm against Bacillus subtilis, 14 mm against Staphylococcus aureus and 19 mm against Escherichia coli. Datura stramonium petroleum ether seed extract produced 16 mm zone of inhibition against Escherichia coli. Datura stramonium aqueous seed extract exhibits 24 mm zone of inhibition against Bacillus subtilis. All the experienced solvent extracts showed potential antimicrobial activity Index against various tested microorganisms. Owning to the results, it can be concluded that the extracts of the Datura stramonium can be used to design different herbal antimicrobial agents.

scholarly journals PREVALENCE OF BACTERIAL PATHOGENS ASSOCIATED WITH WOUND INFECTIONS FROM DIABETIC OUT-PATIENTS AT PLATEAU SPECIALIST HOSPITAL, JOS, NIGERIA

2020 ◽  
Vol 1 (2) ◽  
pp. 1-11
Author(s):  
S. G. Pandukur ◽  
T. T. Sambo ◽  
A. G. Plangnan
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Resistance Pattern ◽  
Klebsiella Pneumonia ◽  
Diabetic Patients ◽  
Bacterial Isolates ◽  
Research Committee ◽  
Laboratory Technology

This study was carried out to determine the frequency and antibiotic susceptibility profile of bacterial agents associated with diabetic patients attending out-patients Department in Plateau Specialist Hospital Jos. Wound swabs and pus were collected from the wounds of 251 out-patients’ department (OPD) over a 14 months’ period. Subjects were recruited upon approval by the Ethical Research Committee of the UCTH and after obtaining written or oral informed consent from the subject. Samples were cultured and microbial isolates identified using standard microbiology methods.  Antibiotic susceptibility testing was carried out on the bacterial isolates. Six bacterial isolates were identified from wounds of diabetic patients viz: Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The most frequently encountered bacterial pathogen of wound infection among subjects was Pseudomonas aeruginosa (26.67%) followed by Staphylococcus aureus (23.33%) while the lowest was Klebsiella pneumonia (6.67%). The antibiotic susceptibility profile of the bacterial isolates to commonly used antibiotics ranged between 0.0% to 100%. The flouroquinolones were the most active antimicrobial agents observed among bacterial isolates from diabetic out-patients studied. S. aureus [7(100%) and 6(85.7%)] was the highest susceptible bacteria followed by P. aeruginosa [7(87.5%) and 5(62.5%)], E. coli 6(100%) against Ciprofloxacin (10%) and Augmentin (30%) respectively. Meanwhile, K. pneumonia and S. pneumonia were not susceptible each (0.0%) against Doxycycline and Chloramphenicol at 10% and 30% concentrations respectively. The study showed a high index of wound contamination with bacteria pathogens and resistance pattern to standard and commonly used antibacterial agents among diabetic out-patient from the study area. Pandukur, S. G. | Department of Science Laboratory Technology, Faculty of Natural Sciences, University of Jos.

scholarly journals Antibacterial and Phytochemical Properties of Crude Leaf Extracts of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on Some Clinical Bacterial Isolates in Nigeria

2020 ◽  
pp. 1-15
Author(s):  
Reginald C. Njokuocha ◽  
Anthonia E. Ewenike
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Bacterial Isolates ◽  
Leaf Extracts ◽  
Ceiba Pentandra ◽  
Ethanolic Extracts ◽  
Pterocarpus Santalinoides

Aims: The study was carried out to determine the phytochemical constituents and antibacterial activity of aqueous and ethanolic extracts of fresh leaves of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on bacterial isolates; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Methodology: The plant leaves were dried, pulverized and phytochemical tests were done according to standard laboratory procedure. Aqueous and ethanolic extracts were obtained from 20 g of the of the ground leaves. Antibacterial assay was carried out with Disc diffusion method on seven concentrations of the extracts ;100,50,25,12.5, 6.25,3.125,1.5625 mg/ml and compared with standard antibiotics. Isolated bacterial pathogens; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (1.0 x 105 cfu /ml) were used as test organisms. Results: Alkaloids, steroidal aglycones, glycosides, proteins, carbohydrates, reducing sugars, tannins, saponins, vitamins A and E were present in all the plant samples. Flavonoids and cardiac glycosides were not detected in Pterocarpus santalinoides and Ceiba pentandra, respectively. Anthracene glycoside was absent in all samples. Aqueous and ethanolic extracts of M. oleifera showed antibacterial activities against all the bacterial isolates at minimum inhibitory concentration (MIC) of 3.125 mg/ml and 1.5625 mg/ml respectively. Pterocarpus santalinoides showed inhibitory activity only on Salmonella typhi at 3.125 mg/ml and Escherichia coli 1.5625 mg/ml MIC. Ceiba pentandra showed spectrum of antibacterial activity against all the bacterial isolates at 1.56 mg/ml MIC with exception of Salmonella typhi. E. coli was the most susceptible to the leaf extracts. Salmonella typhi was not sensitive to the leaf extracts of Ceiba pentandra, while Staphylococcus aureus and Pseudomonas aeruginosa were not sensitive to the leaf extracts of Pterocarpus santalinoides. Conclusion: It can be concluded that both aqueous and ethanolic leaf extracts had antibacterial activity against the test organism, thus justifying their use in folklore medicine.

Endogenous methicillin resistant staphylococcus aureus as a source of post operative surgical site infections

2020 ◽  
pp. 1-12
Author(s):  
Umme Farwa ◽  
Irum Aftab ◽  
Fatima Kaleem ◽  
Mehnaz Khattak ◽  
Mariam Danish Iqbal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Empirical Therapy ◽  
Surgical Site Infections ◽  
Sampling Technique ◽  
Site Infection ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Time Of Admission

Abstract Objectives: To determine frequency of Endogenous Methicillin Resistant Staphylococcus aureus (MRSA) in pre operative patients and its frequency in Surgical Site Infections (SSIs) Post operatively. Methods: It was a descriptive cross sectional conducted at Department of Microbiology, Fauji Foundation Hospital Rawalpindi (FFH), Pakistan. Samples were collected from 1st November-31st May 2018. Total 75 samples were collected during the period. Consecutive non-probability sampling technique was utilized. Specimens were collected from nose, axilla and groin of preoperative patients. Methicillin Resistant Staphylococcus aureus was identified if only isolated from these sites. Patients were followed till his/her discharge from the hospital and if they developed infection post operatively, pus specimen from infected site was also collected and identified.Results: Out of 75 specimen preoperatively, 14.7% (n=11) were identified as endogenous MRSA. From these, 44% (n=33) developed Surgical site infections (SSIs), among them 57% (n=19) were MRSA (09 endogenous, 10 exogenous), 21.2% (n=7) were Escherichia coli, 9.1% (n=3) were Klebsiella pneumoniae, 9.1% (n=3) were Enterococcus faecalis and 3% (n=1) was Methicillin Sensitive Staphylococcus aureus. Conclusion:  The results of this study determined that Endogenous Methicillin Resistant Staphylococcus aureus (MRSA) could be isolated from patients going for surgery if microbiological screening was done at the time of admission. This could prevent patients from Surgical Site Infection Post operatively by these endogenous MRSA. This search and wipe out strategy is able to curtail the events of outbreak, reduce hospital stay and decrease budget of the hospital by providing guidance in choice of empirical therapy for infection. Continuous...

scholarly journals Microbiology of Incisional Surgical Site Infection

2019 ◽  
Vol 17 (1) ◽  
pp. 18-24
Author(s):  
Krisna Rani Majumder ◽  
Samia Mubin ◽  
Md Ibrahim Siddique ◽  
M Nur E Elahi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Surgical Site Infection ◽  
Gastrointestinal Surgery ◽  
Resistance Pattern ◽  
International Studies ◽  
Site Infection ◽  
Prevention Policy ◽  
Pseudomonas Spp ◽  
Incisional Surgical Site Infection

Background: Bacteria responsible for causing lncisional Surgical Site Infection (ISSI) along with their resistance pattern changes over time due to various factors and are a matter of regular scrutiny. Proper understanding of this will help both surgeon and microbiologist to formulate an effective guideline to combat overall SSI. Objectives: To evaluate responsible microorganisms and their resistance pattern causing ISSI following elective gastrointestinal surgery in our perspective. Methods: Prospective data were collected on 1122 surgical patients admitted in the surgery department in BSMMU from January 201 O to June 2012 having elective gastrointestinal surgery. Patients operated were followed in the post operative period till discharge and if any ISSI noted, swab from the site of infection was sent for culture and sensitivity reporting using standard bacteriological techniques. Antibiotics were given accordingly. Results: In this series 1122 wounds were studied of which183(16.31%) cases had ISSI.Wound infection rates, according to clinical wound types were 10.45%, 14.49%, 49% for clean-contaminated,contaminated and dirty wounds respectively. The figures of ISSI according to SSI types were 76.77% for Superficial SSI and 26.23% for Deep SSI.The figures of ISSI according to SSI types were 76.77% for Superficial SSI and 26.23% for Deep SSI. Causative microorganisms were revealed in 74.87% positive cultures. 5.8% wounds had polymicrobial etiology. The common causative organisms were Escherichia coli 55.5%,Staphylococcus aureus 22.6%, Pseudomonas spp. 9.5% and Klebsiella spp. 6.6%.AII were 100% sensitive to Meropenem. Next to this Escherichia coli and Klebsiellaspp. were mostly sensitive to Ceftriaxone 62.5% and 100% respectively, Staphylococcus aureus to Doxicycline 75% and Pseudomonas spp. to Ceftazidime 93.7%. Comparison with previously done national and international studies revealed alarming increase in resistance pattern of causative bacterial isolates. Conclusion: To date no national or in house SSI prevention policy or surveillance guideline exists. Lack of our attention in this issue along with inept, irrational use of antibiotics is definitely adding causative organisms to gain increasing resistance pattern. Before surgical care centers are overwhelmed with SSls by resistant organisms caution must be taken from all level. Journal of Surgical Sciences (2013) Vol. 17 (1) : 18-24

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