Effect of gallic acid on renal biochemical alterations in male Wistar rats                 induced by ferric nitriloacetic acid

The present study is an effort to identify a potent chemopreventive agent against various diseases (including cancer) in which oxidative stress and cell proliferation plays an important causative role. This study was designed to investigate the effect of gallic acid against ferric nitrilotriacetic acid (Fe-NTA)-induced carcinogen/drug metabolizing phase I and phase II enzymes, anti-oxidative parameters, kidney markers, tumour promotion markers and lipid peroxidation (LPO) in kidney of male Wistar rats. Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) caused significant depletion in the detoxification and antioxidant enzyme armoury with concomitant elevation in renal LPO, serum creatinine, blood urea nitrogen, hydrogen peroxide generation, ornithine decarboxylase activity and [3H]thymidine incorporation into renal DNA. However, pretreatment of animals with gallic acid (10 and 20 mg/kg body weight) resulted in a significant decrease in the levels of the parameters measured (P < 0.001). Renal glutathione content (P < 0.001), glutathione metabolizing enzyme (P < 0.001) and antioxidant enzyme levels were also recovered to a significant level (P < 0.001). The enhanced reduced glutathione level and enzyme activities involved in xenobiotic metabolism and maintaining antioxidant status of cells are suggestive of a chemopreventive efficacy of gallic acid against Fe-NTA-mediated oxidative stress, toxicity and cell proliferative response in Wistar rats.

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Effects of Consumption of Rooibos (Aspalathus linearis) and a Rooibos-Derived Commercial Supplement on Hepatic Tissue Injury bytert-Butyl Hydroperoxide in Wistar Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/716832 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

B. D. Canda ◽

O. O. Oguntibeju ◽

J. L. Marnewick

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Wistar Rats ◽

Tissue Injury ◽

Body Weight Gain ◽

The Body ◽

Hepatic Tissue ◽

Herbal Tea ◽

Butyl Hydroperoxide ◽

Male Wistar Rats

This study investigated the antioxidative effect of rooibos herbal tea and a rooibos-derived commercial supplement ontert-butyl hydroperoxide- (t-BHP-) induced oxidative stress in the liver. Forty male Wistar rats consumed fermented rooibos, unfermented rooibos, a rooibos-derived commercial supplement, or water for 10 weeks, while oxidative stress was induced during the last 2 weeks via intraperitoneal injection of 30 µmole oft-BHP per 100 g body weight. None of the beverages impaired the body weight gain of the respective animals. Rats consuming the rooibos-derived commercial supplement had the highest (P<0.05) daily total polyphenol intake (169 mg/day) followed by rats consuming the unfermented rooibos (93.4 mg/day) and fermented rooibos (73.1 mg/day). Intake of both the derived supplement and unfermented rooibos restored thet-BHP-induced reduction and increased (P<0.05) the antioxidant capacity status of the liver, while not impacting on lipid peroxidation. The rooibos herbal tea did not affect the hepatic antioxidant enzymes, except fermented rooibos that caused a decrease (P<0.05) in superoxide dismutase activity. This study confirms rooibos herbal tea as good dietary antioxidant sources and, in conjunction with its many other components, offers a significantly enhanced antioxidant status of the liver in an induced oxidative stress situation.

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Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0132 ◽

2016 ◽

Vol 0 (0) ◽

Author(s):

Ramalingam Sripradha ◽

Magadi Gopalakrishna Sridhar ◽

Nachimuthu Maithilikarpagaselvi

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Food Intake ◽

Wistar Rats ◽

Density Lipoprotein ◽

Mitogen Activated Protein Kinase ◽

Stress Index ◽

Redox Imbalance ◽

Hydroxycitric Acid ◽

Male Wistar Rats

AbstractExcess fructose consumption causes dyslipidemia, oxidative stress, and various complications. Hydroxycitric acid (HCA), one of the principal components of the fruitMale Wistar rats (n=40) were randomly divided into four groups with 10 rats in each group. The rats were fed with either standard rodent diet or 60% fructose diet and administered with HCA at a dose of 400 mg/kg body wt/day for 10 weeks. Body weight was measured once a week, and food intake was noted daily. At the end of the study, lipid profile and oxidative stress parameters were estimated. Expressions of stress sensitive kinases were analyzed in liver homogenates.Fructose-fed rats displayed elevated body weight, higher levels of plasma total cholesterol (TC), triacylglycerol (TAG), non-high-density lipoprotein cholesterol (non HDL-C), malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), lower levels of HDL-C, glutathione (GSH), glutathione peroxidase (GPx), and total antioxidant status (TAS). Fructose feeding caused higher phosphorylation of stress sensitive kinases ERK ½ and p38. Administration with HCA lowered body weight, food intake, TAG, non-HDL-C, MDA, TOS, and OSI and elevated GSH, GPx, and TAS levels. Reduced phosphorylation of ERK ½ and p38 mitogen-activated protein kinase (MAPK) was observed upon HCA treatment.Thus, HCA improved fructose induced redox imbalance and activation of stress sensitive kinases through its hypolipidemic effects.

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Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

Nutrients ◽

10.3390/nu13061999 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1999

Author(s):

Lidia V. Kravchenko ◽

Ilya V. Aksenov ◽

Nikolay S. Nikitin ◽

Galina V. Guseva ◽

Ludmila I. Avrenyeva ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Liver Disease ◽

Lipoic Acid ◽

Lipid Accumulation ◽

Wistar Rats ◽

Weight Ratio ◽

Body Weight Ratio ◽

Deficient Diet ◽

Male Wistar Rats

Non-alcoholic fatty liver disease (NAFLD) is currently estimated as the most prevalent chronic liver disease in all age groups. An increasing body of evidence obtained in experimental and clinical data indicates that oxidative stress is the most important pathogenic factor in the development of NAFLD. The study aimed to investigate the impact of α-lipoic acid (LA), widely used as an antioxidant, on the effects of a hypercaloric choline-deficient diet. Male Wistar rats were divided into three groups: control diet (C); hypercaloric choline-deficient diet (HCCD), and hypercaloric choline-deficient diet with α-lipoic acid (HCCD+LA). Supplementation of HCCD with LA for eight weeks led to a decrease in visceral adipose tissue/body weight ratio, the activity of liver glutathione peroxidase and paraoxonase-1, plasma, and liver total antioxidant activity, as well as an increase in liver/body weight ratio, liver total lipid and triglyceride content, and liver transaminase activities compared to the HCCD group without LA. In conclusion, our study shows that α-lipoic acid detains obesity development but exacerbates the severity of diet-induced oxidative stress and lipid accumulation in the liver of male Wistar rats fed a hypercaloric choline-deficient diet.

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Chronic exposure of adult male Wistar rats to bisphenol A causes testicular oxidative stress: Role of gallic acid

Endocrine Regulations ◽

10.2478/enr-2020-0003 ◽

2020 ◽

Vol 54 (1) ◽

pp. 14-21 ◽

Cited By ~ 2

Author(s):

Samuel Gbadebo Olukole ◽

Eunice Olufunke Ola-Davies ◽

Damilare Olaniyi Lanipekun ◽

Bankole Olusiji Oke

Keyword(s):

Oxidative Stress ◽

Bisphenol A ◽

Gallic Acid ◽

Adult Male ◽

Chronic Exposure ◽

Wistar Rats ◽

The Body ◽

Male Rats ◽

Male Wistar Rats

AbstractObjectives. Bisphenol A (BPA) has been reported that among other male reproductive dys-functions, it can cause marked estrogenic effects including alteration in serum hormones as well as testicular lesions in exposed animals. This work sought to study the role of gallic acid (GA), a known antioxidant, on the BPA-induced testicular oxidative stress in adult male Wistar rats using serum hormone analysis, histopathology, and biochemical assays.Methods. Adult male rats were divided into four groups (n=10) including control (0.2 ml of corn oil), GA (20 mg/kg/day), BPA (10 mg/kg/day), BPA+GA (BPA, 10 mg/kg/day + GA, 20 mg/kg/day). All medications were given by oral gavage for 45 consecutive days. The body and testicular weights were measured. Blood and organ samples were collected for the serum hormonal assay: testosterone (T), luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL), and tissue biochemistry analysis: superoxide dismutase (SOD), reduced glutathione (GSH), glutathione-S-transferase (GST), malondialdehyde (MDA), hydrogen peroxide (H2O2), respectively.Results. The BPA-treated rats showed significant reduction in the gonadosomatic index. BPA also caused significant decrease in the levels of the serum testosterone and prolactin. Furthermore, BPA induced testicular oxidative stress by decreasing the activities of antioxidant enzymes and increasing reactive oxygen species. However, co-treatment with GA protected against these alterations.Conclusion. Findings from the present study confirmed the previously reported data and show that the ability of GA, as a potent antioxidant, may protect against BPA-induced alterations in the male reproductive function. Hence, GA protects against testicular oxidative stress in adult male Wistar rats following chronic exposure to BPA.

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Administration of ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) stem decreased reactive oxygen species, visceral fat and body weight of obese rats

Neurologico Spinale Medico Chirurgico ◽

10.36444/nsmc.v4i1.150 ◽

2021 ◽

Vol 4 (1) ◽

pp. 32-36

Author(s):

Vany Novanty ◽

Wimpie Pangkahila ◽

Ni Nyoman Ayu Dewi

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Visceral Fat ◽

Wistar Rats ◽

Control Group ◽

Final Body Weight ◽

Obese Rats ◽

Male Wistar Rats ◽

Stem Extract ◽

Obese Male

Background: Oxidative stress plays a role in the obesity mechanism, thus leads to premature aging. High antioxidant capacity in Bajakah tampala stem may effectively lessen oxidative stress and reduce fat mass and body weight accordingly. This study aimed to provide Bajakah tampala stem extract's effect in lowering ROS level, visceral fat weight, and overall weight of obese male Wistar rats. Method: A true experimental design was conducted on male Wistar rats aged 2-3 months with obesity. Thirty-two obese rats were evenly divided into a placebo group and a group given Bajakah tampala stem extract, with 16 rats in each group. For 28 days, both groups were fed a high-fat diet. The subject body weights were weighed every week. ROS levels and visceral fat weight were evaluated after the intervention was done. Comparative analysis between groups was performed. Results: The results showed mean levels of ROS (56.2 ± 7.4 U/ml vs. 400.9 ± 50.7 U/ml; p < 0.001), visceral fat weight (2.6 ± 0.2 g vs. 3.4 ± 0.9 g; p < 0.001), and the final body weight (241.5 ± 2.8 g vs. 261.5 ± 13.8 g; p < 0.001) were significantly lower in the study group than the control group. Conclusion: The study indicates Bajakah tampala stem extract administration effectively reduced ROS levels, visceral fat weight, and body weight in obese male Wistar rats.

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Sub-acute evaluation of artesunate, amodiaquine and their combination on muscular coordination and morphology of the cerebellum of Wistar rats

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v14i5.1 ◽

2020 ◽

Vol 14 (5) ◽

pp. 1498-1510

Author(s):

AbdulGafar N. Popoola ◽

Innocent O. Imosemi

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Wistar Rats ◽

Motor Coordination ◽

Artemisinin Combination Therapy ◽

Acute Effect ◽

Water Control ◽

Oxidative Markers ◽

Male Wistar Rats ◽

Immunohistochemical Studies

Artemisinin Combination Therapy (ACT) is currently the best choice for the treatment of malaria. Artesunate-amodiaquine combination is one of the most commonly used ACTs in Nigeria with a very potent efficacy but not without side effects involving motor coordination. Currently, there is dearth of information on its sub-acute effect on the cerebellum. This study investigated the sub-acute effects of artesunate (AS), amodiaquine (AQ) and artesunate + amodiaquine combination (AS+AQ) on the motor coordination, oxidative markers and morphology of the cerebellum of adult Wistar rats. Forty adult male Wistar rats were randomly divided into 4 groups, n=10. The rats were administered orally for 3 days, distilled water (control, CT), 4 mg/kg body weight AS, 10 mg/kg body weight AQ and 4 mg/kg AS + 10 mg/kg AQ. Rats were weighed, assessed for motor coordination and sacrificed on day 15. Brain tissues were excised and some cerebella preserved for oxidative stress and antioxidants while others were fixed in 10% NBF for histological and immunohistochemical studies. Results showed significant decreased body weight of the AQ group compared to the CT; decreased time of fore limb grip in the AS and AQ groups compared with the CT. Test groups showed increased LPO and NO, and lower levels of SOD, CAT and GSH compared with the control. Artesunate and AQ had significantly reduced number of Purkinje cells, AQ and AS caused increased astrocyte population and increased expression of iNOS. Sub-acute studies of Wistar rats treated with artesunate, amodiaquine and their combination induced oxidative stress, reduced their motor function and impaired morphology of the cerebellum. Keywords: Artesunate, amodiaquine, behaviour, cerebellum, histology, immunohistochemistry.

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Aortic-banding induces myocardial oxidative stress and changes in concentration and activity of antioxidants in male Wistar rats

Life Sciences ◽

10.1016/j.lfs.2006.07.015 ◽

2006 ◽

Vol 79 (23) ◽

pp. 2187-2193 ◽

Cited By ~ 16

Author(s):

Maria H.V.M. Jacob ◽

Mauro R.N. Pontes ◽

Alex S.R. Araújo ◽

Jaqueline Barp ◽

Maria C. Irigoyen ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Aortic Banding ◽

Myocardial Oxidative Stress ◽

Male Wistar Rats

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Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

African Journal of Food, Agriculture, Nutrition and Development ◽

10.18697/ajfand.95.19950 ◽

2020 ◽

Vol 20 (07) ◽

pp. 16984-16996

Author(s):

MMC Anyakudo ◽

◽

DO Adeniji ◽

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Treated Group ◽

Morphological Features ◽

High Protein ◽

Control Group ◽

Formulated Diet ◽

Low Carbohydrate ◽

Male Wistar Rats ◽

And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

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