Cytokine expression profiles of patients with acute myelogenous leukemia (AML) and non-Hodgkin lymphoma (NHL) detected by multiplex assay

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 18530-18530
Author(s):  
V. B. Reddy ◽  
D. K. Oelschlager ◽  
J. S. Nolan ◽  
K. L. Taylor ◽  
J. Post ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Recovery Phase ◽  
Cytokine Expression ◽  
Multiplex Assay ◽  
Myelogenous Leukemia ◽  
Control Group ◽  
Serum Samples ◽  
Expression Levels ◽  
Significant Difference ◽  
Low Levels

18530 Background: To determine the cytokine expression profiles of patients with AML and NHL using a sensitive bead-based Luminex multiplex assay in a routine clinical diagnostic setting. Methods: Blood (plasma/serum) samples were collected from ten AML and five NHL patients. Six control samples from patients diagnosed as non-neoplastic/non-autoimmune/non-inflammatory were also analyzed for comparison. All samples were frozen prior to analysis. Using a bead-based Luminex assay (Human Cytokine 8-Plex Assay, Bio- Rad, Hercules, CA) we analyzed these samples for a panel of cytokines (IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, and TNF-alpha). This assay uses polystyrene microspheres, which provides simultaneous quantitation of these cytokines in a single sample. The expression levels were presented in picograms/mL. Average values for each of these markers were obtained for each group of patients (AML versus NHL versus Controls), and their expression levels were compared using χ2 analysis. Results: Overall, there was a significant difference in the expression profiles of all these cytokines among three patients groups (χ2, P < 0.001). All cytokines were consistently expressed at low levels in NHL patients as compared to control group. However, the levels of IL-6 and IL-8 were increased by 2.7 and 5.8 times, respectively in AML patients as compared to controls. Conclusions: The low levels of cytokines in NHL and AML patients suggest suppressed immune system in these two disease conditions; however, these findings warrant further studies to explore the underlying mechanisms for the increased levels of IL-6 and IL-8 in AML patients. Currently, studies are in progress to compare the levels of cytokines measured by Luminiex assay in different stages of leukemias and lymphomas (initial, post treatment and recovery phase etc.). These studies are partially funded by grants from the National Institute of Health/National Cancer Institute (RO1-CA98932–01 and U24-CA086359). No significant financial relationships to disclose.

Three superoxide dismutase genes from Tetranychus cinnabarinus (Boisduval) involved in the responses to temperature and acaricide stresses

2019 ◽  
Vol 24 (1) ◽  
pp. 16 ◽  
Author(s):  
Tianrong Xin ◽  
Xiaoyue Li ◽  
Jiadong Yin ◽  
Xianyan Ye ◽  
Ji Wang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Metal Binding ◽  
Expression Profiles ◽  
Treated Group ◽  
The Other ◽  
Control Group ◽  
Tetranychus Cinnabarinus ◽  
Expression Levels ◽  
Relative Expression ◽  
Significant Difference

In almost all aerobic organisms, the superoxide dismutase (SOD) is considered as an important antioxidant enzyme regulating oxidative stress. Tetranychus cinnabarinus is an economically important polyphagous pest mite, which harms a variety of economic crops and ornamental plants. In the present study, the full-length cDNA sequences of cytoplasmic Cu/ZnSOD (TcSOD1), extracellular Cu/ZnSOD (TcSOD2) and mitochondrial MnSOD (TcSOD3) from T. cinnabarinus were cloned by combining RT-PCR and rapid amplification of cDNA ends (RACE). The corresponding open reading frames (ORFs) encode three putative polypeptides of 152, 232, 225 amino acid residues, respectively. These sequences share the conserved SOD functional domains, signature motifs and metal binding sites. Multiple alignment analysis revealed that cytosolic Cu/ZnSOD and mitochondrial MnSOD sequences are relatively conserved, while extracellular Cu/ZnSODs are more diverse. Phylogenetic analysis showed that SODs are organized into two major clades, corresponding to Cu/ZnSODs, and MnSODs. Cu/ZnSODs are subdivided into two branches, one being composed of cytoplasmic Cu/ZnSODs, and the other corresponding to extracellular Cu/ZnSODs. Expression profiles of the three genes were determined at different temperatures (4°C, 25°C, and 40°C) for 2 hours. The relative expression of TcSOD1, TcSOD2, and TcSOD3 were significantly down-regulated (0.344-, 0.287-, and 0.358-fold, respectively) at 4°C compared to 25°C (P<0.05). The relative expression levels of TcSOD1 and TcSOD2 genes were significantly down-regulated at 40°C (0.481- and 0.291-fold less than in the control group, respectively) (P<0.05), while there was no significant difference in the relative expression level of TcSOD3(P>0.05). Moreover, expression levels were altered after exposition to different acaricides. TcSOD1, TcSOD2, and TcSOD3 were significantly down-regulated (0.450-, 0.147- and 0.663-fold decreases, respectively) in the abamectin-treated group (P<0.05). TcSOD1 and TcSOD2 were down-regulated, in the fenpropathrin-treated group with 0.794- and 0.201-fold decreases, respectively. On the other hand, the expression of TcSOD3 was significantly increased (P<0.05), being 2.774-fold higher than in the control group. The expression of TcSOD2 was significantly down-regulated both the propargite- and cyflumetofen-treated groups (0.655- and 0.397-fold, respectively) (P<0.05). The data reported here indicate that SODs from T. cinnabarinus may play different and vital roles in anticipating the effects of oxidative damage at extreme temperatures and under different acaricides stress.

Dasatinib Promotes the Maturation of Healthy Donors and Chronic Myelogenous Leukemia Patients Derived Dendritic Cells

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 53-54
Author(s):  
Wangjun Cao ◽  
Lin He ◽  
Hong Zheng ◽  
Xiaobing Huang ◽  
Xi Yang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Chronic Myelogenous Leukemia ◽  
Myelogenous Leukemia ◽  
Control Group ◽  
Surface Markers ◽  
Expression Levels ◽  
Hla Dr ◽  
Healthy Donors ◽  
Significant Difference ◽  
Enhanced Expression

Dasatinib has been discovered to obtain immunomodulatory effects in addition to the targeting effect towards BCR-ABL in chronic myelogenous leukemia (CML) patients. But the effect of dasatinib on dendritic cells (DCs) is still not fully understood. Objective To investigate the effect of dasatinib on the maturation of monocyte derived dendritic cells (moDCs) from healthy donors (HDs) and CML patients. Method Peripheral blood mononuclear cells (PBMCs) were isolated from HDs (n=14) and CML patients (n=14) who had got remission of MR4.5 with imatinib treatment. The generation of moDCs was completed after 7 days of incubation in DC I medium, and another 3 days of incubation in DC II medium with or without 25nM dasatinib. On the 10th day, the moDCs were harvested and analyzed for the expression of surface markers CD83, CD80, CD86, CD40 and HLA-DR to reflect the maturation of the moDCs by flow cytometry. Results When analyzing the moDCs from all the 14 HDs together, dasatinib significantly enhanced the expression levels of the surface marker CD83 (P=0.039), CD40 (P=0.002) and HLA-DR (P=0.000) on moDCs compared with the control group, but there was no statistically significant difference in CD80 (P=0.073) and CD86 (P=0.897). Differently, when analyzing the moDCs derived from all the 14 patients together, there was no statistically significant difference in the expression levels of CD80 (P=0.086), CD86 (P=0.166), CD83 (P=0.674), CD40 (P=0.574) and HLA-DR (P=0.561) on moDCs between the dasatinib group and the control group. However, moDCs derived from 3 of the 14 patients show the enhanced expression of all the five surface makers with dasatinib administration compared to the control group. Besides, the moDCs derived from 6 of the 14 patients show the enhanced expression of at least one of the five surface markers. Notably, compared with moDCs derived from HD group (n=14), moDCs from patient group (n=14) express lower levels of CD80 (P=0.340), CD86 (P=0.007), CD40 (P=0.010), CD83 (P=0.019) and HLA-DR (P=0.036). Conclusion Dasatinib at the concentration of 25nM can efficiently promote the maturation of HD-derived moDCs in vitro, whereas this effect only occurs in part of the CML patients. And the moDCs from patients generally show much lower levels of the maturation associated surface makers compared with moDCs from HDs. Dasatinib shows potential as a DC adjuvant to be applied in DC-based therapeutic strategies, such as DC vaccine and DC cell-therapy. Disclosures No relevant conflicts of interest to declare.

scholarly journals Comparison of Cytokine Expression Profile in Chikungunya and Dengue Co-Infected and Mono-Infected Patients’ Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 166
Author(s):  
Saravana Murali Krishnan ◽  
Jayashri Mahalingam ◽  
Shanthi Sabarimurugan ◽  
Thiruvengadam Muthu ◽  
Baskar Venkidasamy ◽  
...  
Keyword(s):  
Viral Load ◽  
Expression Profiles ◽  
Febrile Illness ◽  
Cytokine Expression ◽  
Rt Pcr ◽  
Serum Samples ◽  
Cytokine Expression Profile ◽  
Significant Difference ◽  
Ifn Γ

Chikungunya (CHIKV) and Dengue (DENV) viruses cause an acute febrile illness which is hard to clinically differentiate and treat since both exhibit similar symptoms. Hence, this study was aimed at identifying the expression profiles of cytokines on co-infected samples and compare with CHIKV and DENV mono-infected samples. Serum samples of 292 suspected patients during 2009–2011 were analyzed. The presence of primary (IgM)/secondary (IgG) antibodies and early NS1 Dengue antigens were confirmed by capture ELISA. Molecular diagnosis and serotypes were discriminated by RT-PCR, confirmed by sequencing. All the plasma samples were assayed for cytokine expression by BDTM cytometry bead array (CBA) and compared with independent mono-infection viral load. Among the tested samples, 82 were confirmed as Dengue positive; 52 through IgM (17.8%), and 30 through IgG (10.2%). Additionally, 186 samples were confirmed as Chikungunya, 96 through IgM (32.6%) and 92 through IgG (31.5%) ELISA, respectively. Interestingly, 19 patients were co-infection positive in which, only 6 were confirmed for CHIKV and 7 for DENV by RT-PCR. Among 8 cytokines, IL-2, IL-8, IFNα, IFN γ, and IL-12 were found to be significantly different between co-infected and CHIKV mono-infected patients and correlated with viral load. DENV viral load was correlated with cytokine expression and a significant difference in IL-2 and IL-12 was observed between DENV mono-infected and DENV and CHIKV co-infected patients. Results indicated that apart from serological and molecular confirmation, cytokines could be used as a specific biomarker for the diagnosis of DENV and CHIKV. In the future, the role of independent cytokines can be determined to understand the pathogenesis and etiology of these dreadful diseases.

scholarly journals Expression of AIM2 in rheumatoid arthritis and its role on fibroblast-like synoviocyte

2020 ◽  
Author(s):  
fujuan qiu ◽  
Chen Yong ◽  
Qiu Fujuan ◽  
Zhao Xiaofeng ◽  
Xiao Changhong
Keyword(s):  
Rheumatoid Arthritis ◽  
Mtt Assay ◽  
Control Group ◽  
Expression Levels ◽  
Potential Target ◽  
Caspase 1 ◽  
Significant Difference ◽  
And Migration ◽  
Fibroblast Like Synoviocytes ◽  
Proliferation And Migration

Abstract Background To determine whether any differences of AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and investigate the effects of AIM2 when transferred into RA fibroblast-like synoviocytes (RA-FLS).Methods Serum AIM2 levels between OA and RA patients were compared by ELISA. Different expression levels of AIM2, ASC, Caspase-1 and IL-1β between RA and OA synovium were semi-quantified by RT-qPCR and immunohistochemical (IHC) staining. IHC staining were recorded by H scores, and determine the correlation with ESR and CRP levels of RA patients. SiRNA AIM2 was transferred to RA-FLS and observe its effects on proliferation and migration by MTT assay and transwell test respectively.Results In RA sera, no significant difference was observed between OA and RA patients. However, in affected knee synovium, AIM2, ASC, Caspase-1 and IL-1β were expressed higher in RA than that of OA. Plus, H score of AIM2, ASC, and IL-1β were positively correlated to ESR and CRP levels in RA patients. After transferred AIM2 siRNA to FLS and incubation for 48 hours, the proliferation of FLS were significantly inhibited, and the apoptosis rate were significantly increased compared to FLS in control group. However, no effect on migration was detected.Conclusions AIM2 participated in the proliferation of FLS, and might be a potential target for therapy.

scholarly journals Examining Metabolic Profiles in Opioid-‎Dependent Patients ‎

2020 ◽  
Vol 10 (3) ◽  
pp. 28681
Author(s):  
Nader Molavi ◽  
Amir Ghaderi ◽  
Hamid Reza Banafshe
Keyword(s):  
Control Group ◽  
Study Group ◽  
Metabolic Profiles ◽  
Opioid Use ◽  
Serum Samples ◽  
Social Burden ◽  
Study Results ◽  
Significant Difference ◽  
Hs Crp ◽  
Opioid Users

Background: Drug abuse is a social burden and a public health disorder. Previous evidence suggested numerous illicit substances (e.g., opioids, amphetamines, cocaine, & cannabis) affect immune system functions, oxidative stress mechanisms, inflammatory cytokines, and reactive oxygen species production. This study aimed to determine the extent of these metabolic parameters in opioid-dependent patients. We also compared these patients with a healthy control group. Methods: This study was conducted in Amirie Clinic, Kashan, Iran. Plasma and serum samples from 50 illicit opioid users (study group) and 50 non-opioid users (control group) were studied. Metabolic levels for MDA, NO, TAC, GSH, Insulin, HOMA-IR, and hs-CRP were assessed in both research groups (N=100). Results: There was a significant difference in the status of MDA (P=0.003), NO (P=0.01), TAC (P=0.003), GSH (P=0.001), insulin (P=0.04), HOMA-IR (P=0.02), and hs-CRP (P=0.001) between the study and control groups. Furthermore, there was a significant correlation among the duration of illicit opioid use and MDA concentrations (r=-0.424, P=0.002), as well as TAC levels (r=0.314, P=0.02). Conclusion: The study results suggested metabolic profiles were impaired in the study group, compared to the controls.  

scholarly journals Expression profiles of glutathione S-transferases genes in semi-engorged Haemaphysalis longicornis (Acari: Ixodidae) exposed to Cymbopogon citratus essential oil

2020 ◽  
Author(s):  
Desmond Onyeka Agwunobi ◽  
Tingwei Pei ◽  
Xiaoshuang Wang ◽  
Zhijun Yu ◽  
Jing-Ze Liu
Keyword(s):  
Essential Oil ◽  
Pacific Islands ◽  
Expression Profiles ◽  
Effective Control ◽  
Control Group ◽  
Domestic Sheep ◽  
Haemaphysalis Longicornis ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Glutathione S Transferases

Abstract Background: The tick Haemaphysalis longicornis is well known as vector of several zoonotic pathogens responsible for various clinical conditions, increasingly threatens the veterinary and public health. It is mainly distributed in East Asia, New Zealand, Australia, and several Pacific islands, and has been expanded rapidly in United States since its first founding on a nonimported domestic sheep in New Jersey. Glutathione S-transferases (GSTs) are phase II detoxification enzymes, which function via combining with pesticidal molecules and catalyzing the conjugation of molecules by thiol of glutathione, so as to protect tissues from oxidative stress damage. In the tick H. longicornis, glutathione S-transferases (HlGST and HlGST2) have been previously identified. However, the relationship between the expression of glutathione S-transferases and the essential oil treatment in ticks remains unexplored. Hence, in the present study, the expression profiles of HlGST and HlGST2 mRNAs were evaluated in H. longicornis after exposure to Cymbopogon citratus essential oil. Results: At 24 h post-exposure of H. longicornis to different sublethal concentrations of C. citratus essential oil, ANOVA results revealed significant difference (F2,6 = 55.94, P = 0.0001) in the expression of HlGST. Tukey’s test showed that HlGST was significantly induced after treatment with 1% C. citratus essential oil (P = 0.0002); whereas no significant difference (P = 0.3551) was detected after treated by 2% C. citratus essential oil. No significant difference (F2,6 = 0.8990, P = 0.4555) in the expression of HlGST2 between the treatment and the control group of 50% ethanol. Nevertheless, the under-expression of HlGST2 in the treatment groups versus the untreated control group was not significant (F3,8 = 2.643, P = 0.1208). Conclusion: The results implied that GST mRNA is a potential molecular target for C. citratus essential oil in H. longicornis. Further understanding of the underlying mechanisms of the GST at the molecular level could contribute to develop effective control measures for ticks and tick-borne diseases.

Can IL-2R Alpha be a Valuable Marker along with Ca 19–9 in the Diagnosis of Chronic Pancreatitis and Pancreatic Cancer?

2004 ◽  
Vol 19 (3) ◽  
pp. 196-202
Author(s):  
B. Kayhan ◽  
B. Kayhan ◽  
M. Akdoğ;an
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Interleukin 2 ◽  
Carbohydrate Antigen ◽  
Control Group ◽  
Serum Samples ◽  
Cancer Group ◽  
Abdominal Symptoms ◽  
Significant Difference ◽  
Inflammatory Processes

Background Pancreatic cancer is characterized initially by non-specific abdominal symptoms followed by rapid tumor progression. Although chronic pancreatitis is a benign disorder, it can be one of the causative factors of pancreatic cancer. The level of the tumor marker carbohydrate antigen 19–9 (CA 19–9) in pancreatic cancer does not correlate with the stage of the neoplasm. Soluble interleukin 2 receptor (sIL-2R) is a cytokine that shows increased levels during some inflammatory processes and malignant disorders. Aim Our aim in this study was to investigate whether sIL-2Rα levels can be used in association with CA 19–9 in the early diagnosis of pancreatic cancer and chronic pancreatitis. Patients Serum samples were obtained from the blood of 21 pancreatic cancer patients without distant metastasis who were deemed inoperable, 16 chronic pancreatitis patients and 20 normal volunteers. Results We did not find any significant differences in CA 19–9 levels between normal controls and patients with chronic pancreatitis. There was a significant difference in the levels between the control group and the pancreatic cancer group (p=0.003) and between patients with chronic pancreatitis and those with pancreatic cancer (p=0.004). Although there was no significant difference in sIL-2Rα levels between the control group and the patient groups, we found a slight correlation between sIL-2Rα and CA 19–9 levels in the pancreatic cancer group (p=0.003, r=0.623) and a more marked correlation in the chronic pancreatitis group (p<0.01, r=0.751). Conclusion According to our results, sIL-2Rα alone is not a good candidate marker in the diagnosis of pancreatic cancer; it can, however, be used in association with CA 19–9 for this purpose.

scholarly journals Network Structure Analysis Identifying Key Genes of Autism and Its Mechanism

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yanhui Wang ◽  
Yanming Kou ◽  
Dazhi Meng
Keyword(s):  
Expression Profiles ◽  
Structural Parameters ◽  
Enrichment Analysis ◽  
Average Degree ◽  
Control Group ◽  
Original Research ◽  
Cardiovascular Development ◽  
Correlation Networks ◽  
Significant Difference ◽  
Key Genes

Identifying the key genes of autism is of great significance for understanding its pathogenesis and improving the clinical level of medicine. In this paper, we use the structural parameters (average degree) of gene correlation networks to identify genes related to autism and study its pathogenesis. Based on the gene expression profiles of 82 autistic patients (the experimental group, E) and 64 healthy persons (the control group, C) in NCBI database, spearman correlation networks are established, and their average degrees under different thresholds are analyzed. It is found that average degrees of C and E are basically separable at the full thresholds. This indicates that there is a clear difference between the network structures of C and E, and it also suggests that this difference is related to the mechanism of disease. By annotating and enrichment analysis of the first 20 genes (MD-Gs) with significant difference in the average degree, we find that they are significantly related to gland development, cardiovascular development, and embryogenesis of nervous system, which support the results in Alter et al.’s original research. In addition, FIGF and CSF3 may play an important role in the mechanism of autism.

scholarly journals Folate-receptor 1 level in periodontal disease: a pilot study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Duygu Alkan ◽  
Berrak Guven ◽  
Cigdem Coskun Turer ◽  
Umut Balli ◽  
Murat Can
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Folate Receptor ◽  
Serum Folate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Samples ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Significant Difference

Abstract Background The purpose of this study was to investigate gingival crevicular fluid (GCF) and serum folate-receptor 1 (FOLR1) levels in subjects with different periodontal status. Methods The study consists of three groups: Healthy group (n = 15), gingivitis group (n = 15) and chronic periodontitis group (n = 15). Clinical periodontal parameters including probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI) and bleeding on probing (BOP) were assessed. GCF and serum samples were collected from each patient and were analyzed FOLR1 levels by enzyme-linked immunosorbent assay. Results The values of FOLR1 in GCF were higher in gingivitis and periodontitis groups than among patient in control group (p < 0.016). Serum FOLR1 levels showed no significant difference between the groups. A significant correlation was observed between FOLR1 levels of GCF and BOP (p < 0.05). Conclusions Our preliminary data suggest that FOLR1 is not useful in monitoring the periodontal disease. Further studies are necessary to clarify the role, regulation and function of folate and it’s receptors in the pathogenesis of periodontal disease.

scholarly journals Correlation between expression levels of IL-37, GM-CSF, and CRP in peripheral blood and atherosclerosis and plaque stability

2019 ◽  
Vol 17 ◽  
pp. 205873921984406
Author(s):  
Tao Zheng ◽  
Qingyun Zhou ◽  
Zhe Chen ◽  
Qinning Wang
Keyword(s):  
Peripheral Blood ◽  
Density Lipoprotein ◽  
Inflammatory Factors ◽  
Control Group ◽  
Case Group ◽  
Expression Levels ◽  
Gm Csf ◽  
Significant Difference ◽  
Group A ◽  
Group B

The study aimed to study the correlation between expression levels of interleukin-37 (IL-37), granulocyte macrophage colony-stimulating factor (GM-CSF), and C-reactive protein (CRP) in peripheral blood and the status of atherosclerosis (AS) and plaque stability and to confirm the clinical significance of these inflammatory factors in the pathogenesis of AS. A total of 64 AS patients (case group) were selected and divided into unstable plaque group (group A, 28 cases) and stable plaque group (group B, 36 cases) according to the color ultrasonography results of arterial vessels. At the same time, 30 healthy subjects were classified into the control group. General information of the enrolled subjects was collected, including levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), CRP, and homocysteine (Hcy). The expression levels of IL-37 and GM-CSF in the serum of peripheral blood samples collected from these subjects were measured by enzyme-linked immunosorbent assay (ELISA). There was no significant difference between the case group and the control group in the levels of TC, TG, HDL, and LDL ( P > 0.05). However, the expression level of Hcy in the case group was significantly higher than that in the control group ( P < 0.05). Compared with the control group, the expression levels of IL-37, GM-CSF, and CRP in the case group were significantly increased ( P < 0.05). In addition, compared with group B, the expression level of GM-CSF in group A was significantly increased ( P < 0.05), while no significant difference was detected between group A and group B in the expression levels of IL-37 and CRP ( P > 0.05). In conclusion, inflammatory factors IL-37, GM-CSF, CRP, and Hcy were all involved in the pathogenesis of AS, and the increased levels of GM-CSF were closely related to the progress of unstable plaques. These results may aid the early diagnosis/treatment of AS.

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