scholarly journals Concomitant caries and calculus formation from in situ dentin caries model

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 3
Author(s):  
Frederico B de Sousa ◽  
Pablo J Mangueira ◽  
David R Tames ◽  
Sandra S Vianna ◽  
Neriede S Santos-Magalhaes
Keyword(s):  
Sucrose Solution ◽  
Dental Calculus ◽  
Backscattered Electrons ◽  
Dental Tissues ◽  
Dentin Caries ◽  
Caries Model ◽  
Calculus Formation ◽  
Hard Dental Tissues ◽  
Short Time

The aim of this study was to test the possibility of the concomitant formation of calculus deposits and caries fromin situdentin caries model for short time periods. Six volunteers wore palatal removal appliances with four polished dentin specimens protected from intra-oral mechanical forces for up to 14 days. Each volunteer applied a 50% sucrose solution (four times a day) on the specimens and performed a daily mouthwash with 0.05% NaF. Samples were removed after 2, 5, 9 and 14 daysin situ. Demineralization was analyzed by stereomicroscopy and SEM (secondary electrons and backscattered electrons modes) and calculus was analyzed by energy dispersive spectroscopy and fluorescence spectroscopy. Seventeen samples, at least one sample from each volunteer, presented dental calculus on both carious and non-carious ones, detected in all time intervals. Ca/P ratios of dental calculus ranged from 1.1 to 1.7. Some large calculus deposits on carious surfaces were confirmed by fluorescence. In conclusion, concomitant caries and calculus formation can be found in dentin caries formedin situ. This has important repercussions on the study of surface phenomena on the interface between hard dental tissues and dental plaque.

Calcium Prerinse before Fluoride Rinse Reduces Enamel Demineralization: An in situ Caries Study

2016 ◽  
Vol 50 (4) ◽  
pp. 372-377 ◽  
Author(s):  
João Gabriel S. Souza ◽  
Livia Maria Andaló Tenuta ◽  
Altair Antoninha Del Bel Cury ◽  
Diego Figueiredo Nóbrega ◽  
Renan R. Budin ◽  
...  
Keyword(s):  
Sucrose Solution ◽  
Surface Hardness ◽  
Sodium Lactate ◽  
Negative Control ◽  
Distilled Water ◽  
Double Blind ◽  
Caries Model ◽  
Biofilm Fluid ◽  
Water Rinse

A calcium (Ca) prerinse before a fluoride (F) rinse has been shown to increase oral F levels. We tested the anticaries effect of this combination in a dose-response in situ caries model. In a double-blind, crossover experiment, 10 volunteers carried enamel slabs in palatal appliances for 14 days, during which they rinsed twice/day with one of four rinse combinations: (1) a placebo prerinse (150 mM sodium lactate) followed by a distilled water rinse (negative control); (2) a placebo prerinse followed by a 250 ppm F rinse; (3) a placebo prerinse followed by a 1,000 ppm F rinse, or (4) a Ca prerinse (150 mM Ca, as calcium lactate) followed by a 250 ppm F rinse. Sucrose solution was dripped onto the slabs 8×/day to simulate a high cariogenic challenge. The percent surface hardness loss (%SHL) was significantly lower in the Ca prerinse used with the 250 ppm F rinse group (%SHL = 38.0 ± 21.0) when compared with the F rinse alone (%SHL = 59.5 ± 24.1) and similar to the 1,000 ppm F rinse group (%SHL = 42.0 ± 18.3). Compared with the 250 ppm F rinse, the Ca prerinse increased biofilm fluid F only twice (nonsignificant). However, it greatly increased F in biofilm solids (∼22×). The Ca prerinse had little effect on loosely or firmly bound enamel F. The results showed an increased level of protection against demineralization by the use of a Ca prerinse, which seems to be caused by the enhancement of F concentration in the biofilm.

Effects on Demineralization of Enamel by Fluoridated Sucrose: A Pilot Study in an in Situ Caries Model

1995 ◽  
Vol 9 (1) ◽  
pp. 9-13 ◽  
Author(s):  
P. Carlsson ◽  
B. Angmar-Mansson ◽  
I.-M. Redmo-Emanuelsson ◽  
K. Anderssén
Keyword(s):  
Pilot Study ◽  
Sucrose Solution ◽  
Sugar Content ◽  
Dry Weight ◽  
Thin Sections ◽  
Human Enamel ◽  
Caries Model ◽  
Weight One ◽  
Mineral Loss

Blocks of human enamel, placed in removable partial dentures, were allowed to acquire natural plaque for seven days and were exposed extra-orally to a cariogenic challenge by repeated periods in a fluoride-sucrose solution. As a control, enamel blocks were exposed extra-orally to a sucrose solution. After two weeks of cariogenic challenge, the blocks were examined for mineral loss by quantitative microradiography on thin sections of the enamel. The results from six subjects showed that no significant effect on demineralization could be detected by the addition of fluoride corresponding to fluoride/sugar content of 1 mg/kg, 5 mg/kg, or 10 mg/kg (dry weight). One subject did not develop lesions at all, either with fluoride-sucrose or with sucrose exposure alone.

scholarly journals Frequency of sucrose exposure on the cariogenicity of a biofilm-caries model

2016 ◽  
Vol 10 (03) ◽  
pp. 345-350 ◽  
Author(s):  
Natalia Díaz-Garrido ◽  
Carla Lozano ◽  
Rodrigo A. Giacaman
Keyword(s):  
Protein Content ◽  
Streptococcus Mutans ◽  
Surface Hardness ◽  
Dependent Manner ◽  
Human Diet ◽  
Dental Tissues ◽  
Unexposed Control ◽  
Caries Model ◽  
Viable Bacteria ◽  
Hard Dental Tissues

ABSTRACT Objective: Although sucrose is considered the most cariogenic carbohydrate in the human diet, the question of how many exposures are needed to induce damage on the hard dental tissues remains unclear. To approach this question, different frequencies of daily sucrose exposure were tested on a relevant biological caries model. Materials and Methods: Biofilms of the Streptococcus mutans were formed on enamel slabs and exposed to cariogenic challenges with 10% sucrose for 5 min at 0, 1, 3, 5, 8, or 10 times per day. After 5 days, biofilms were retrieved to analyze biomass, protein content, viable bacteria, and polysaccharide formation. Enamel demineralization was evaluated by percentage of microhardness loss (percentage surface hardness loss [%SHL]). Results: Biomass, protein content, polysaccharide production, acidogenicity of the biofilm, and %SHL proportionally increased with the number of daily exposures to sucrose (P < 0.05). One daily sucrose exposure was enough to induce 20% more demineralization than the negative unexposed control. Higher frequencies induced greater demineralization and more virulent biofilms, but eight and ten exposures were not different between them in most of the analyzed variables (P > 0.05). Conclusions: Higher sucrose exposure seems to increase cariogenicity, in a frequency-dependent manner, by the modification of bacterial virulent properties.

scholarly journals Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2558
Author(s):  
Mihaela Olaru ◽  
Liliana Sachelarie ◽  
Gabriela Calin
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Present Review ◽  
Modern Concept ◽  
Dental Tissues ◽  
Tissue Engineering Approach ◽  
Hard Dental Tissues ◽  
Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

NbC and VC Nanoparticles Reinforced Fe-Si-Mn Matrix Composite In Situ Synthesized by Plasma Jet

2011 ◽  
Vol 415-417 ◽  
pp. 71-75
Author(s):  
Chun Xiang Cui ◽  
Yan Chun Li ◽  
Tie Bao Wang ◽  
Shuang Jin Liu ◽  
Suek Bong Kang
Keyword(s):  
Matrix Composite ◽  
Hybrid Composite ◽  
Gas Flow ◽  
Plasma Jet ◽  
Homogeneous Distribution ◽  
Active Nitrogen ◽  
Ceramic Particles ◽  
Nanometer Range ◽  
Short Time

In situ NbC and VC nanoparticles reinforced Fe-Si-Mn-Nb-V matrix composite was carried out using a plasma jet with a plasma gas flow of (Ar + CH4) for very short time. The process involve improving the efficiency of the reaction in terms of consumption of the available active nitrogen atoms as well as the production of very fine and homogeneous distribution of all reinforcing phases of ceramic particles, preferable in the nanometer range. The nanoreinforcements synthesized by in situ reaction in this hybrid composite are NbC and VC ceramic particles.

scholarly journals Composition of dental plaque formed in the presence of sucrose and after its interruption

2003 ◽  
Vol 14 (3) ◽  
pp. 147-152 ◽  
Author(s):  
Jaime Aparecido Cury ◽  
Aline Soler Marques ◽  
Cíntia Pereira Machado Tabchoury ◽  
Altair Antoninha Del Bel Cury
Keyword(s):  
Crossover Study ◽  
Dental Plaque ◽  
Sucrose Solution ◽  
Inorganic Phosphorus ◽  
Deionized Water ◽  
The Other ◽  
Low Concentration ◽  
Human Enamel ◽  
Two Stages

Since dental plaque reservoirs of fluoride (F), calcium (Ca) and inorganic phosphorus (Pi) are susceptible to decreases in pH, this in situ crossover study was conducted to test the hypothesis that the low concentration of these ions in plaque, formed in the presence of sucrose, could be attributed merely to the fermentation of this sugar. Eleven volunteers wore palatal appliances containing 6 human enamel blocks during two stages. In each stage, the treatments were either 20% sucrose solution or distilled deionized water, which were dripped onto the blocks 8 times a day. After 28 days, in each stage, the dental plaque formed on two blocks was collected, the treatment was inverted and after a further 24 and 48 h, the biofilm formed was collected from the other blocks. The concentration of acid-soluble F, Ca and Pi, and the concentration of insoluble polysaccharide (IP) were determined in the dental plaque. Statistically lower concentrations of F, Ca and Pi, and a higher concentration of IP were found in the 28-day biofilm formed in the presence of sucrose than in its absence; after the treatment inversion the change in F, Ca and Pi was not statistically significant, but the IP concentration changed significantly. The hypothesis was rejected because change in concentration of F, Ca and Pi is not due to fermentation of the sucrose.

scholarly journals Do dental calculi predict the presence of renal stones?

2018 ◽  
Vol 90 (3) ◽  
pp. 159-162 ◽  
Author(s):  
Bulent Kati ◽  
Ergin Kalkan ◽  
Eyyup Sabri Pelit ◽  
Ismail Yagmur ◽  
Halil Çiftçi
Keyword(s):  
Kidney Stones ◽  
Stone Formation ◽  
Renal Stones ◽  
The Body ◽  
Dental Calculus ◽  
Recurrence Rates ◽  
Urinary Ph ◽  
Dental Hospital ◽  
Calculus Formation ◽  
The Relationship

Objective: Pathological calcifications that occur in various parts of the body may cause stone formation over time. The structure of these stones is similar in many regions of the body. We have studied the relationship between dental calculi and kidney stones. Material and methods: A total of 183 patients with dental stone complaints or dental calculi were included between April and August 2016 in the Cagri Dental Hospital, Elazig, Turkey. Patients were evaluated with regard to a urinary tract ultrasonography, urinalysis, oral hygiene, and stone and surgical disease history. All information was statistically investigated. Results: The age of the patients in the kidney stones group was significantly higher than the non-kidney stone patients (p < 0.05). In the group with kidney stones, the percentage of dental calculus formation was significantly higher than the group without stones (p < 0.05). In the groups with and without kidney stones, dental stone recurrence rates did not differ significantly (p < 0.05). Urinary pH was significantly lower in the group with stones than the group without stones (p < 0.05). Conclusions: During a physical examination, the formation of a visible stone, such as a dental calculus, may be an indicator of other types of stones, such as kidney stones, and this should be further investigated.

scholarly journals DIAGNOSTIC AND PROGNOSTIC SIGNIFICANCE OF MICROBIAL FLORA IMBALANCE IN GINGIVAL BIOFILM

2020 ◽  
Vol 5 (2) ◽  
pp. 76-82
Author(s):  
G. A. Loban ◽  
T. O. Petrushanko ◽  
V. V. Chereda ◽  
M. O. Faustova ◽  
M. M. Ananieva ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Inflammatory Diseases ◽  
Periodontal Diseases ◽  
Prognostic Significance ◽  
Fluid Composition ◽  
Dental Tissues ◽  
Periodontal Tissues ◽  
Dmf Index ◽  
Increased Risk ◽  
Hard Dental Tissues

Background. Periodontal tissues inflammatory diseases are widespread among young people. Objective. This study was aimed at elaborating the method to assess risks of periodontal inflammatory diseases and determining its efficacy depending on the state of dental tissues, gum tissues and sex.          Methods. The study included 182 students (93 men, 89 women) aged 19-29: 22 individuals had no lesions of hard dental tissues and no signs of periodontal disease; 51 individuals were found to have DMF index <6; 52 individuals – DMF index ≥6; 57 individuals were diagnosed with chronic catarrhal gingivitis. Primary groups were formed in autumn; re-examination was carried in spring. The research participants were assessed for detection of risks of periodontal inflammatory disease by the method developes by the authors (Patent UA 54041). Results. The study revealed that the risk of development of preiodontitis increases in individuals with high caries and gingivitis intensity. In spring, more individuals suffer from microbial imbalance in in the composition of gingival sulcus fluid and decrease in the mean stability coefficient value that indicates an increased risk of inflammatory periodontal disease development. Women were less likely to experience seasonal dysbiotic changes in the gingival sulcus fluid composition compared with men. Conclusions. The method suggested for assessment of the risk of periodontal inflammatory diseases is of high informativeness. It allows clinicians detecting early pre-nosological signs of oral microbiocenosis imbalance that enhances the effectiveness of early diagnosis of inflammatory periodontal diseases.

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