scholarly journals Growth and health status of Pangasionodon hypophthalmus reared under manipulated photoperiod conditions

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 154
Author(s):  
Windarti Windarti ◽  
Bintal Amin ◽  
Asmika H. Simarmata

Background: In general, the length of photoperiod affects the physiology of Pangasionodon hypophthalmus. This study aimed to understand the growth and health status of this fish reared under manipulated photoperiods. Methods: The study was conducted between June to August 2020[A1] . Three treatments were applied: control (natural photoperiod); 18 hours of darkness (18D6L; tanks were placed under a dark tarp tent for 18 hours); and 24 hours of darkness (24D0L; tanks were placed under a dark tarp tent continuously). Three replications were performed per treatment. At baseline, fish were approx. 7.5 cm  [A2] total length (TL) and 4 g body weight (BW), and were reared in circular plastic tanks (100 L; 30 fish/tank) with aerators and filters, and fed with commercial fish feed pellet (2 times/day to satiation). Fish growth and survival were studied once/week, and blood and tissue samples were taken at the end of the experiment (8 th week). Tissue was formalin fixed and HE stained. Results: The survival of fish in all treatment was 100%. The fish reared in 24D0L and 18D6L grew better than control, achieving mean TL of 23 cm and BW of 98 g (control = 19 cm TL and 72 g BW). There was no difference in hematology condition or tissue structure between the three groups. Tissue structure of gill, kidney and liver were normal, but light abnormality due to parasites was present in the gill of fish reared in 24D0L. Blood samples for all three groups showed mean red blood cell count of 1,800,000 cells/ml and white blood cell (WBC) count of 55,200 cells/ml. WBC consisted of lymphocyte 65%, monocyte 24%, thrombocyte 6%, neutrophil 3%, eosinophil 1% and basophile 1%. Conclusion: Data obtained indicate that a short photoperiod improves the growth of P. hypophthalmus fish and does not negatively affects their health.

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 154
Author(s):  
Windarti Windarti ◽  
Bintal Amin ◽  
Asmika H. Simarmata

Background: In general, the length of photoperiod affects the physiology of Pangasionodon hypophthalmus. This study aimed to understand the growth and health status of this fish reared under manipulated photoperiods. Methods: The study was conducted between May and August 2020. Three treatments were applied: control (natural photoperiod); 18 hours of darkness (18D6L; tanks were placed under a dark tarp tent for 18 hours); and 24 hours of darkness (24D0L; tanks were placed under a dark tarp tent continuously). Three replications were performed per treatment. At baseline, fish were approx. 3 inch total length (TL) and 4 g body weight (BW), and were reared in circular plastic tanks (100 L; 30 fish/tank) with aerators and filters, and fed with commercial fish feed pellet (2 times/day to satiation). Fish growth and survival were studied once/week, and blood and tissue samples were taken at the end of the experiment (8th week). Tissue was formalin fixed and HE stained. Results: The survival of fish in all treatment was 100%. The fish reared in 24D0L and 18D6L grew better than control, achieving a mean TL of 23 cm and BW of 98 g (control = 19 cm TL and 72 g BW). There was no difference in hematology condition or tissue structure between the three groups. Tissue structure of gill, kidney and liver were normal, but light abnormality due to parasites was present in the gill of fish reared in 24D0L. Blood samples for all three groups showed mean red blood cell count of 1,800,000 cells/ml and white blood cell (WBC) count of 55,200 cells/ml. WBC consisted of lymphocyte 65%, monocyte 24%, thrombocyte 6%, neutrophil 3%, eosinophil 1% and basophile 1%. Conclusion: Data obtained indicate that a short photoperiod improves the growth of P. hypophthalmus fish and does not negatively affects their health.


2021 ◽  
Vol 11 (12) ◽  
pp. 5681
Author(s):  
Paraskevi Stathopoulou ◽  
Evangelia Tsoumalakou ◽  
Efi Levizou ◽  
Theofilos Vanikiotis ◽  
Stefanos Zaoutsos ◽  
...  

Aquaponics provides an alternative opportunity for the combined production of fish and plants. Most of the essential nutrients required for optimal plant growth can be supplied from the fish feed, except for K and Fe. These nutrients are usually inadequate in fish feed. In this study, red tilapia and rocket plants were co-cultivated in an aquaponics system along with the extra addition of K and Fe. Fish growth, morpho-anatomical characteristics, and K and Fe loading on fish gills were studied. Plant growth parameters were also determined. The addition of Fe and K slightly improved the produced fresh biomass of rocket per unit area and had no impact on tilapia growth and survival. No severe histological alterations in fish gills, liver, and midgut were detected. EDX analysis showed that the addition of K led to the enrichment of this specific ion in fish gills, but no effects of this accumulation were found on other aspects of fish growth and survival.


2021 ◽  
Vol 883 (1) ◽  
pp. 012076
Author(s):  
S N Pratama ◽  
R C Mukti

Abstract The feed is one of the most important factors in the growth and survival of fish. One of the efforts made to increase the growth and survival of fish is the provision of herbal supplements in fish feed. The research aim is to provide information about the utilization of herbal supplements on the growth and survival of catfish (Clarias sp). This research has been carried out from July to August 2020 at Bandar Agung Lahat Village, South Sumatera. Catfish rearing containers use two waring units placed in concrete ponds with two treatment that P0: control (without herbal supplements) and P1: utilization of herbal supplements and. The data taken includes absolute growth and absolute length, survival, feed efficiency, and water quality. The utilization of herbal supplements has a good effect on fish growth, feed efficiency, and survival rate. Maintenance of P0 (control) resulted in an absolute weight of 9.79 grams, an absolute length of 6.3 cm, the survival of 85.15%, feed efficiency of 107.89% while maintenance of P1 with the utilization of herbal supplements resulted in an absolute weight of 14.17 grams, an absolute length 7.8 cm, survival 98.57%, feed efficiency 161.170%.


2020 ◽  
Vol 6 (1) ◽  
pp. 21-24
Author(s):  
Usy Nora Manurung ◽  
Edwin Oscar Langi

Bakasang, produk fermentasi dari jeroan ikan, mengandung Bakteri Asam Laktat (BAL) yang beberapa diantaranya tergolong bakteri probiotik yang dapat meningkatkan pertumbuhan ikan. Penelitian ini bertujuan untuk mengkaji pengaruh pakan mengandung bakasang (PMB) terhadap pertumbuhan dan kelangsungan hidup ikan bawal (Colosoma macropomum) dan menentukan dosis paling efektif dari bakasang terhadap pertumbuhan dan kelangsungan hidup ikan bawal. Sebanyak 120 ekor ikan yang ditempatkan dalam 12 hapa dengan kepadatan 10 ekor ikan/hapa. Setelah proses aklimatisasi, ikan uji diberi PMB dengan empat dosis berbeda (A=0 ml/kg pakan, B= 50 ml/kg pakan, C=75 ml/kg pakan dan D=100 ml/kg pakan) dengan tiga ulangan pada masing-masing perlakuan, pada dosis 5%/bb/hari, diberikan dua kali sehari selama 14 hari. Hasil penelitian menunjukan bahwa pengaruh pemberian pakan meningkat bertambahnya konsentrasi dengan pertumbuhan terbaik teramati pada dosis 100 ml/kg pakan dengan bertumbuhan sebesar 8.7 gram. Hasil ini memperlihatkan bahwa bakasang merupakan sumber potential probiotik yang dapat meningkatkan pertumbuhan ikan bawal meskipun pengujian dengan periode pemeliharaan lebih lama perlu dilakukan untuk melihat pengaruh optimum dari PMB terhadap pertumbuhan dan kelangsungan hidup.    Bakasang, a fermentation product made from fish’s viscera, contains Lactic Acid Bacteria (LAB), some of which are probiotic bacteria that can increase fish growth. This study aimed to determine effect of fish feed containing bakasang (FCB) and the effective dose of bakasang on the growth of pomfret (Colosoma macropomum). 120 fish were placed in small net cage (1x1x1) m3. Following acclimatization process, the test fish were fed with four different doses of FCB (A = 0 ml/kg of feed, B = 50 ml/kg of feed, C = 75 ml/kg of feed and D = 100 ml/kg of feed) at a dose of 5% /w/day twice a day in triplicate for 14 days. The results showed that the effect of FCB on the pamfret fish growth increased in a dose dependent manner with best growth observed at a dose of 100 ml/kg of feed with growth of 8.7 gram, suggesting bakasang as potential source of probiotic that can increase the growth of pamfret fish although further study covering longer period time is required to measure the optimum potential of FCB on pamfret fish growth and survival rate.


Angiology ◽  
2021 ◽  
pp. 000331972110211
Author(s):  
Buyun Jia ◽  
Chongfei Jiang ◽  
Yun Song ◽  
Chenfangyuan Duan ◽  
Lishun Liu ◽  
...  

Increased arterial stiffness is highly prevalent in patients with hypertension and is associated with cardiovascular (CV) risk. Increased white blood cell (WBC) counts may also be an independent risk factor for arterial stiffness and CV events. The aim of the study was to investigate the relationship between differential WBC counts and brachial-ankle pulse wave velocity (baPWV) in hypertensive adults. A total of 14 390 participants were included in the final analysis. A multivariate linear regression model was applied for the correlation analysis of WBC count and baPWV. Higher WBC counts were associated with a greater baPWV: adjusted β = 10 (95% CI, 8-13, P < .001). The same significant association was also found when WBC count was assessed as categories or quartiles. In addition, the effect of differential WBC subtypes, including neutrophil count and lymphocyte count on baPWV, showed the similar results. These findings showed that baPWV has positive associations with differential WBC counts in hypertensive adults.


2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Ademola Olusegun Talabi ◽  
Tewogbade Adeoye Adedeji ◽  
Oludayo Adedapo Sowande ◽  
Olusanya Adejuyigbe

Abstract Background The diagnosis of acute appendicitis in children is quite challenging as the rate of negative appendectomy varies between 15 and 57%. Increased utilization of imaging diagnostic facilities in advanced countries seems to have reduced the incidence of operating on normal appendix to a single digit. In low- and middle-income countries, the incidence remains unacceptably high (double digits). Inflammatory markers and scoring systems may be a suitable adjunct to increase diagnostic yield in most third world countries. Thus, the aim of this study was to evaluate the diagnostic value of Alvarado score, white blood cell count, and serum C-reactive protein in children with acute appendicitis. Results The ages of patients ranged between 4 and 15 years with a mean of 11.2 ± 2.8 years. The male to female ratio was 1.4 to 1.0. Nineteen percent of patients had negative appendiceal findings on histological examination. The sensitivity and specificity of Alvarado score, C-reactive protein estimation, total white blood cell count in diagnosing acute appendicitis were 86.4% and 63.2%, 98.8% and 36.8%, and 51.9% and 89.5% respectively. Alvarado score has the highest area under ROC curve analysis 0.824, 95% CI of 0.724 to 0.924 compared with CRP, 0.769. 95% CI of = 0.647 to 0.891 and WBC count, 0.765, 95% CI of 0.643 to 0.887. Both CRP and WBC count showed higher discriminatory values between complicated and uncomplicated appendicitis, p < 0.001. Conclusion Alvarado score outperformed other tests in setting the diagnosis of acute appendicitis. However, none of the tests can be relied on wholly for operative decision. Clinical judgement remains the bedrock for diagnosis and operative management.


2013 ◽  
Vol 04 (S 01) ◽  
pp. S31-S34 ◽  
Author(s):  
Shin Yi Ng ◽  
Ki Jinn Chin ◽  
Tong Kiat Kwek

ABSTRACT Background: Leucopenia has been reported after induction of thiopentone barbiturate therapy for refractory intracranial hypertension. However, the incidence and characterisitics are not well described. Aims: We performed a retrospective review to describe the incidence and characteristics of leucopenia after induction of thiopentone barbiturate therapy. Setting and Design: Our centre is a national referral centre for neurotrauma and surgery in a tertiary medical institution.Materials and Methods: We performed a retrospective review of all patients who received thiopentone barbiturate therapy for refractory intracranial hypertension during an 18 month period from January 2004 to June 2005 in our neurosurgical intensive care unit. Statistical Analysis Used: Statistical analysis was performed using SPSS version 15.0. All data are reported as mean ± standard deviation or median (interquartile range). The Chi square test was used to analyze categorical data and student t test done for comparison of means. For paired data, the paired t?test was used.-test was used. Results: Thirty eight (80.9%) out of 47 patients developed a decrease in white blood cell (WBC) count after induction of thiopentone barbiturate coma. The mean decrease in WBC from baseline to the nadir was 6.4 × 10 9 /L (P <lt; 0.001) and occurred 57 (3-147) h after induction. The mean nadir WBC was 8.6 < 3.6 × 10 9 /L. Three (6.4%) patients were leucopenic, with a WBC count of 2.8, 3.1, and 3.6 < 10 9 /L. None of them were neutropenic. We did not find an association between decrease in WBC count and clinical diagnosis of infection. We did not find any association between possible risk factors such as admission GCS, maximum ICP prior to induction of barbiturate coma, APACHE II score, total duration and dose of thiopentone given, and decrease in WBC count. Conclusions: Decrease in WBC count is common, while development of leucopenia is rare after thiopentone barbiturate coma. Regular monitoring of WBC counts is recommended.


2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Numisye Iske Mose ◽  
Jetti Treslah Saselah

Tanaman wori merupakan salah satu tanaman kelompok fabaceace yang tumbuh melimpah di Sangihe dan sangat potensial digunakan sebagai salah satu sumber pakan ikan. Kadar protein daun wori adalah 25,53% sehingga berpotensi menjadi protein nabati bagi ikan. Kendala utama yang ditemui ketika memanfaatkan daun tanaman wori adalah kandungan karbohidrat yang tinggi yaitu 48,51% sehingga dapat mempengaruhi proses pencernaan ikan, penyerapan nutrisi yang berujung pada rendahnya pertumbuhan ikan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung daun wori femerntasi terhadap laju pertumbuhan spesifik dan sintasan hidup ikan nila. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan yang dilakukan terdiri dari tiga perlakuan dan tiga kali ulangan yaitu kadar tepung daun wori tanpa fermentasi A (kontrol) 10%, kemudian B tepung daun wori fermentasi (10%), dan C tepung daun wori fermentasi (20%). Laju pertumbuhan spesifik dan efisiensi pakan diuji menggunakan ANOVA sedangkan sintasan hidup akan dianalisis secara deskriptif. Hasil penelitian ini menunjukan bahwa penambahan tepung wori fermenasi dalam pakan dapat meningkatkan nilai laju pertumbuhan spesfik ikan nila dan efisiensi pemanfaatan pakan dengan nilai tertinggi pada perlakuan C yaitu SGR 1,94%/hari. Sementara itu, untuk semua perlakuan nilai sintasan hidup sebesar 100%.   The wori plant is one of the plants of the fabaceace group that grows abundantly in Sangihe and has potential to be used as a source of fish feed. The protein content of wori leaves is 25.53%, so it has the potential to become vegetable protein for fish. The main obstacle encountered when utilizing wori plant leaves is the high carbohydrate content of 48.51% so that it can affect the digestion process of fish, nutrient absorption which lead to low fish growth. The purpose of this study was to determine the effect of wori femerntation leaf meal on the specific growth rate and survival of tilapia. The experimental design used was a completely randomized design (CRD). The treatments consisted of three treatments and three replications, namely the content of fermented wori leaf powder, A (control) 10%, then B fermented wori leaf powder (10%), and C fermented wori leaf powder (20%). Specific growth rate and feed efficiency were tested using ANOVA, while survival rate was analyzed descriptively. The results of this study indicate that the addition of fermented wori powder in the feed can increase the value of the specific growth rate of tilapia and feed utilization efficiency with the highest value in treatment C, namely SGR 1.94% / day. Meanwhile, for all treatments the survival rate was 100%.


Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 160
Author(s):  
Aprelia Martina Tomasoa ◽  
Deidy Azhari

Laju pertumbuhan dan tingkat reproduksi yang tinggi merupakan karakteristik unggul yang dimiliki ikan nila sebagai komoditi budidaya. Tingkat reproduksi yang tinggi menyebabkan terjadinya pematangan gonad sebelum mencapai ukuran produksi dimana hal tersebut dapat merugikan pembudidaya, karena saat ikan mengalami matang gonad energi hasil asimilasi pakan sebagain besar akan dialokasikan untuk perkembangan gonad dan menghambat pertumbuhan somatik. Pemanfaatan senyawa aktif dari bahan alami biji pepaya dapat menjadi solusi untuk meningkatkan pertumbuhan ikan yang dibudidayakan sekaligus menjaga keamanan pangan dan konsumen. Penelitian ini bertujuan untuk mengetahui pemanfaatan tepung biji pepaya terhadap respons pertumbuhan dan tingkat kelangsungan hidup ikan nila. Ikan nila diberi perlakuan dengan lima dosis tepung biji pepaya berbeda (0, 1, 3, 5 dan 7 g/kg pakan) secara oral berdasarkan feeding rate 5% dengan tiga ulangan selama 30 hari pemeliharaan. Hasil penelitian menunjukkan dosis 5 g/kg meningkatkan pertumbuhan bobot tubuh tertinggi sebesar 547 g dibandingkan kontrol sebesar 406 g. Sejalan dengan pertumbuhan bobot tubuh, dosis 5 g/kg menghasilkan tingkat kelangsungan hidup ikan nila sebesar 100% dibandingkan kontrol sebesar 66%. Dari hasil yang diperoleh dapat disimpulkan bahwa tepung biji pepaya dengan dosis 5 g/kg pakan dapat meningkatkan pertumbuhan dan tingkat kelangsungan hidup ikan nila selama 30 hariHigh growth rate and high reproductive cycle are some economical triats on nile tilapia. This is a setback for nile tilapia culture because when the fish reach gonad maturity before reach market size, all the energy demand will flow to reproduction and can cause stunt growth. The utilization of natural compound from papaya seeds can be a solution to enhance growth of farmed nile tilapia and for consumer and food safety. This research aim to know how papaya seed powder can enhance growth and survival rate of nile tilapia. This research conducted into five groups of 5 papaya seeds powder dosage (0, 1, 3, 5 and 7 g/kg) that feed orally to the treatment fishes. Result showed the fishes feed with 5 g/kg papaya seed experienced highest growth performance 547 g compare to control which is 406 g. Same as growth performance, survival rate shown that group feed with 5 g/kg papaya seed had 100% survival rate compare with control which is 66% during treatment periods. Base on this result, we can make conclusion that fish feed with 5 g/kg of papaya seed have a better growth performance and survival rate compare with control group for 30 days treatment periods


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