scholarly journals The effect of green tea extract on the quality of sea bass (Dicentrarchus labrax) fillets in cold storage conditions

2020 ◽  
Vol 37 (3) ◽  
pp. 229-236
Author(s):  
Elifcan Duman ◽  
Can Altınelataman
Keyword(s):  
Green Tea ◽  
Dicentrarchus Labrax ◽  
Thiobarbituric Acid ◽  
Sea Bass ◽  
Storage Conditions ◽  
Green Tea Extract ◽  
Control Group ◽  
Color Analysis ◽  
Significant Difference ◽  
Tea Extract

Despite its high nutritional value, antioxidants are used in aquaculture products that have a short lifetime, as in other foods, to maintain quality during preservation. The purpose of this study; It is to observe the oxidative stability of sea bass (Dicentrarchus labrax) fillets at 0-4 ℃ using green tea extract obtained by classical brewing method, which is a method that can be applied by the consumer with ease. Determined concentrations (0.4-0.8-1.2 g / ml) and immersion times (10-20 min) green tea extract is applied and the fillets stored at 0-4 Thiobarbituric acid reagents (TBARs) analysis, sensory analysis and color analysis were performed on days 0, 3, 6 and 9 of storage. Depending on the literature, although no significant effect is expected, total aerobic mesophilic bacteria (TAMB) analysis was also performed additionally. As a result of the analyzes, significant differences were found when the TBARs results of all samples with green tea extract applied were compared with the results of the control group after 9 days of storage (p <0.05). At the end of storage, the 4th group (0.8% g / ml-20min) gave the best results with 0.31 ± 0.009 μ mol MDA / g and there is a significant difference was found between the other groups (p <0.05). Green tea extract; As expected, it was determined that it did not have a significant antimicrobial and sensory effect. The color values caused a negative increase in the a * value, which expresses its green color as expected due to the color substances in its content, and a positive increase in the b * values, which express the yellow color. As a result of this study, it was found that green tea extract can be used as natural antioxidant in sea bass fillets.

scholarly journals Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis)

2007 ◽  
Vol 19 (1) ◽  
Author(s):  
Isti Endah Kurniwati ◽  
Juni Handajani ◽  
Regina TC. Tandelilin
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Dental Plaque ◽  
Health Benefit ◽  
Green Tea Extract ◽  
Control Group ◽  
Research Subjects ◽  
Significant Difference ◽  
Tea Extract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.

scholarly journals Effect of sulcular green tea extract irrigation on experimental rabbit’s gingivitis (A histopathological study)

2020 ◽  
Vol 32 (1) ◽  
pp. 19-27
Author(s):  
Dilyar A Baban
Keyword(s):  
Experimental Study ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Study Group ◽  
Distilled Water ◽  
Cell Counts ◽  
Experimental Gingivitis ◽  
Significant Difference ◽  
Tea Extract

Background: Considering the antioxidant, anti-inflammatory, and antimicrobial properties of green tea, this study aimed to evaluate the histopathological effect of the sulcular irrigation of green tea extract in the treatment of experimental gingivitis in rabbit. Materials and methods: For this experimental study, 45 male rabbits, separated in two groups, control non- irrigated group (5rabbits) and study group (40 rabbits), gingivitis induced by ligatures was packed subgingivally in the lower right central incisors of the experimental group for seven days. Then, the animals were randomly designated to two irrigated groups (20 rabbits for each) with 50µl/kg of distilled water and 5% green tea extract once daily for seven days starting from the day of ligature removal respectively. Specimens have taken at 1, 3, 7, and 14 days for light microscopical evaluation of inflammatory cellular infiltration. Results: Regarding study group (5% of green tea extract), results demonstrated a significant reduction in the mean values of inflammatory cell counts within three days (P≤ 0.05) to reach the amount of control group within seven days (P˃ 0.05). A highly significant difference was detected between control group and study group sprayed with distilled water during all healing periods (P≤ 0.05). Histological examination showed that the resolution of gingival inflammation, re-epithelization, and tissue healing were achieved much quicker in the group of gingival sulcular irrigation with 5% green tea extract. Conclusions: In this experimental study, the histopathological analysis demonstrated that the use of 5% extract of green tea as gingival sulcular irrigation might fasten healing after the induction of gingivitis. Keywords: green tea, animal model, gingival sulcular irrigation, experimental gingivitis.

scholarly journals PERAN TEH HIJAU TERHADAP OVARIUM, ENDOMETRIUM DAN AORTA TIKUS YANG DIPAPAR DEPOMEDROXY PROGESTERONE ACETATE (DMPA)

2021 ◽  
Vol 7 (3) ◽  
pp. 568-575
Author(s):  
Nora Veri
Keyword(s):  
Corpus Luteum ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Tea Extract ◽  
True Experiment

Background: The type of injectable contraceptive that is currently widely used is Depo Medroxyprogesterone Acetate (DMPA) which contains the hormone progestin, which is a synthetic material from progesterone. The use of progestin contraceptives can reduce endometrial proliferation and increase apoptosis in endometrial tissue. Cell apoptosis is triggered by an imbalance between positive signals (cell growth factors) and negative signals (DNA damage and increased levels of oxidants) under conditions of oxidative stress. Apoptosis of oocytes in large numbers will cause tissue damage which is characterized by atrophy of the ovaries and causes decreased reproductive function. Oxidative stress can be overcome with endogenous and exogenous antioxidants to reduce free radicals in the body. Green tea is one of the herbal plants that act as antioxidants and phytoestrogens, through the polyphenol compounds contained therein.Purpose: To know the role of green tea in providing protective effects on the ovaries, endometrium and aorta in rats.Methods : The research design used in this study was a true experiment with a post test only control group design approach. In this study, the researchers treated green tea extract at a dose of 10.8 mg/rat/day, 21.6 mg/rat/day, and 43.2 mg/rat/day and exposure to DMPA at a dose of 2.7 mg/rat/day. Green tea extract was administered orally for 28 days. The research hypothesis is that green tea can increase the number of follicles, the number of endometrial glands and reduce the arteriosclerosis score of blood vessels. The preparations were stained by the Hemotoxin-Eosin (HE) method and using the OlyVIA software.Result: There was a significant decrease in the number of corpus luteum, endometrial glands and arteriosclerosis scores due to DMPA exposure but not on the number of follicles. Green tea extract was able to increase the number of endometrial glands at a dose of 10.8 mg/day, but there was no significant difference between doses. Green tea extract was able to reduce arteriosclerosis scores, but the most effective dose was 43.2 mg/day.Conclusion: DMPA exposure was able to significantly reduce the number of corpus luteum, the number of endometrial glands and arteriosclerosis score, but there was no significant difference in the number of primary, secondary, tertiary and de Graff follicles, there was a difference in the number of corpus luteum in the group exposed to DMPA, green tea extract was able to increase the number of endometrial glands and decrease the arteriosclerosis score.Suugestion It is necessary to determine the phase of the reproductive cycle before calculating the number of follicles and examining the level of the hormone estrogen to determine whether the experimental animal has become a hypoestrogen model or not. Keywords : Progesterone, Contraception, Green Tea, Antioxidant ABSTRAK Latar Belakang : Jenis kontrasepsi suntik yang saat ini banyak digunakan adalah Depo Medroxyprogesterone Acetate (DMPA) yang mengandung hormon progestin yaitu bahan sintestis dari progesteron. Penggunaan kontrasepsi progestin dapat menurunkan proliferasi endometrium dan meningkatkan apoptosis pada jaringan endometrium. Apoptosis sel dipicu karena adanya ketidakseimbangan antara sinyal positif (faktor pertumbuhan sel) dan sinyal negatif (kerusakan DNA dan peningkatan kadar oksidan) pada kondisi stress oksidatif. Apoptosis pada oosit dalam jumlah yang besar akan mengakibatkan kerusakan jaringan yang ditandai dengan terjadinya atrofi pada ovarium dan menyebabkan fungsi reproduksi menurun. Stress oksidatif dapat diatasi dengan antioksidan endogen dan eksogen untuk menurunkan radikal bebas dalam tubuh. Teh hijau merupakan salah satu tanaman herbal yang berperan sebagai antioksidan dan fitoestrogen, melalui senyawa polifenol yang terkandung didalamnya.Tujuan : Mengetahui peran teh hijau dalam meberikan efek protektif terhadap ovarium, endometrium dan aorta pada tikus.Metode : Rancangan penelitian yang digunakan dalam penelitian ini adalah true experiment dengan pendekatan post test only control group design. Dalam penelitian ini perlakuan peneliti adalah pemberian ekstrak teh hijau dengan dosis 10,8 mg/tikus/hari, 21,6 mg/tikus/hari, dan 43,2 mg/tikus/hari dan paparan DMPA dosis 2,7 mg/tikus/hari pada tikus. Ekstrak teh Hijau diberikan secara oral selama 28 hari. Hipotesis penelitian adalah teh hijau mampu meningkatkan jumlah folikel, jumlah kelenjar endometrium dan menurunkan skor arteriosklerosis pembuluh darah. Preparat diwarnai dengan metode Hemotoxin-Eosin (HE) dan menggunakan software OlyVIA.Hasil : Terjadi penurunan jumlah korpus luteum, kelenjar endometrium dan skor arteriosklerosis akibat paparan DMPA secara bermakna namun tidak pada jumlah folikel. Ekstrak teh hijau mampu meningkatkan jumlah kelenjar endometrium pada dosis 10.8 mg/hari, namun tidak ada perbedaan yang signifikan antar dosis. Ekstrak teh hijau mampu menurunkan skor arteriosklerosis, namun yang paling efektif adalah dosis 43.2 mg/hari.Kesimpulan : Paparan DMPA mampu menurunkan jumlah korpus luteum, jumlah kelenjar endometrium dan skor arteriosklerosis secara bermakna, namun tidak ada perbedaan yang bermakna pada jumlah folikle primer, sekunder, tersier dan de Graff, ada perbedaan jumlah korpus luteum pada kelompok yang dipapar DMPA, ekstrak teh hijau mampu meningkatkan jumlah kelenjar endometrium  dan menurunkan skor arteriosklerosis.Saran Perlu menentukan fase siklus reproduksi sebelum dilakukan perhitungan jumlah folikel dan pemeriksaan kadar hormon estrogen untuk menentukan apakah hewan coba sudah menjadi model hypoestrogen atau belum. Kata Kunci :  DMPA, Kontrasepsi, Teh Hijau, Antioksidan 

scholarly journals The Differences of Malondialdehyde Serum Level, Expression of Tumor Necrosis Factor Alpha and Vascular Endothelial Growth Factor, and the Area of Endometriotic Implants in Administration of Kebar Grass Extract (Biophytum petersianum) and Green Tea Extract (Camelia sinensis) to Mice

2019 ◽  
Vol 24 (3) ◽  
pp. 169
Author(s):  
Yuli Trisetiyono ◽  
Noor Pramono ◽  
Syarief Thaufik Hidayat ◽  
Widjiati Widjiati
Keyword(s):  
Oxidative Stress ◽  
Serum Level ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Vegf Expression ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Significant Difference ◽  
Tea Extract

The pathological pathway of endometriosis remains unclear and involves complex etiologies. Increased oxidative stress is understood to be related to this disease. Oxidative stress produces reactive oxygen species, causes inflammation that is characterized by recruiting lymphocytes and phagocyte activation, produces cytokines that induce oxidation enzyme, and supports epithelium growth. Oxidative stress conjointly will increase angiogenesis and promotes the proliferation of endometriosis tissue within the peritoneal cavity. Kebar grass and green tea contain high antioxidants, are expected to extend antioxidant defense leading to reduced oxidative stress, inflammation, angiogenesis, and reduced endometriosis tissue implants. The objective is to analyze the consequences of Kebar grass and green tea extract to MDA serum level, TNF-α, and VEGF expression, and the area of the endometriotic implants in the mice models.  The study was an experiment designed. It has been conducted within the Department of Obstetrics Gynecology, Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Twenty-one mice were divided into three groups, i.e., the first group of mouse models was given Kebar extract 3 mg/day; the second group was assigned green tea extract 1.1 mg/day; therefore the third group was a control group contains the untreated endometriosis mice. Each treatment was given for fourteen days. MDA serum level was measured by specto-photometric examination, the area of the endometriotic implants was measured by computer tracing technique, whereas TNF-α and VEGF expression of endometriotic implants were measured by IHC using Rammele Scale Index (ImmunoReactive Score). The MDA serum levels of the groups treated with Kebar grass extract and green tea extract were significantly lower than the control group (0.09±0.022 mmol, 0.07±0.019 mmol, and 0.30±0.062 mmol, respectively; p=0.001). TNF-α expression of the groups supplied with each treatment also lower than the control groups (2.43±1.521, 3.66±1.422, and 7.26±2.898, respectively; p=0.002). However, VEGF expression was not significantly different between Kebar grass extract group, green tea group, and the control (4.34±2.402, 4.57±1.998, 7.40±3.495, respectively; p=0.089). Finally, the area of the endometriotic implants of the mice models administered with all treatment was smaller than the control group (0.01±0.025 mm2, 8.76±18.776 mm2, and 34.80±13.079 mm2, respectively; p=0.003). Conclusion: Kebar grass extract, as well as green tea extract administration to endometriosis model mice, resulted in lower MDA serum level and TNF-α expression, smaller the area of endometriotic implants compared, but not resulted in a significant difference of VEGF expression.

scholarly journals GO NATURAL, SAY NO TO CHEMICALS - A SYSTEMATIC REVIEW ON EFFECTIVENESS OF GREEN TEA EXTRACT CONTAINING FORMULATIONS ON DENTAL CARIES

2019 ◽  
pp. 63-69 ◽  
Author(s):  
JAYASHRI PRABAKAR ◽  
JOSEPH JOHN ◽  
MEIGNANA ARUMUGHAM I ◽  
PRADEEP KUMAR R ◽  
SRISAKTHI D
Keyword(s):  
Systematic Review ◽  
Dental Caries ◽  
Green Tea ◽  
Bactericidal Effect ◽  
Green Tea Extract ◽  
Control Group ◽  
Eligibility Criteria ◽  
Caries Increment ◽  
Significant Difference ◽  
Tea Extract

Objective: In recent years, the side effects encountered with the use of chemicals has led to the search for safe anticariogenic alternatives. Green tea is one such naturally occurring alternative which possesses anticariogenic activity through a direct bactericidal effect against cariogenic microorganisms. Therefore, the objective of this systematic review was to analyze the existing literature on the effectiveness of green tea extract containing formulations on dental caries. Methods: The databases of PubMed, Cochrane, Science Direct, Metapress, and Google Scholar were searched up to July 2015 for the related topic. References of the selected articles and relevant reviews were searched for any missed publications. Results: The systematic search revealed a total of 437 publications which were scrutinized based on eligibility criteria. Six publications fulfilled the criteria and 431 publications were excluded from the review. Out of six studies, three studies reported a statistically significant difference in favor of green tea while two studies showed no statistically significant difference between the groups. One study reported no statistically significant difference between the two groups; however, the reduction observed in chlorhexidine (control group) was slightly greater than green tea. Conclusion: With the available evidence, it can be concluded that green tea extract containing formulations are effective in reducing cariogenic microflora and caries increment.

scholarly journals Effect of supplementation with rumen-protected choline and green tea extract on production performance of transition Karan Fries cows

2020 ◽  
Vol 13 (3) ◽  
pp. 489-494
Author(s):  
Parag Acharya ◽  
S. S. Lathwal ◽  
Pawan Singh ◽  
Neela Madhav Patnaik ◽  
Baisakhi Moharana
Keyword(s):  
Green Tea ◽  
Milk Yield ◽  
Milk Fat ◽  
Basal Diet ◽  
Green Tea Extract ◽  
Production Performance ◽  
Control Group ◽  
Significant Difference ◽  
Tea Extract ◽  
Karan Fries

Aim: The main objective of this study was to estimate the effect of supplementation of rumen-protected choline (RPC) and green tea extract (GTE) on production parameters in transition Karan Fries (KF) cows. Materials and Methods: The present experiment was carried out on 32 pregnant KF cows. In the control group, cows were fed basal diet. In T1, each cow was fed RPC (55 g/day), in T2 – GTE (3 g/d), and in T3 – RPC + GTE (55+3) g/day along with basal diet. The duration of the experiment was 30 days before calving to 60 days after parturition. Results: Feeding of both RPC and GTE significantly increased milk yield (p≤0.01), 4% fat corrected milk yield (p≤0.01), milk fat (p≤0.01), and total solid content (p≤0.05) than control. There was no significant difference (p≥0.05) in milk protein, lactose, and solids not fat ( SNF) content among the groups. Conclusion: Supplementing RPC and GTE in combination improved milk yield and fat content of the milk without altering protein, lactose content of the milk in transition KF cows.

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

2020 ◽  
Vol 21 (1) ◽  
pp. 31-35
Author(s):  
Basma El-Desoky ◽  
Shaimaa El-Sayed ◽  
El-Said El-Said
Keyword(s):  
Gene Expression ◽  
Single Dose ◽  
Green Tea ◽  
Serum Testosterone ◽  
Green Tea Extract ◽  
Male Rats ◽  
Controlled Study ◽  
Control Group ◽  
Testicular Damage ◽  
Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

scholarly journals Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

2013 ◽  
Vol 49 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Tariq Mahmood ◽  
Naveed Akhtar ◽  
Barkat Ali Khan ◽  
Akhtar Rasul ◽  
Haji M. Shoaib Khan
Keyword(s):  
Green Tea ◽  
Storage Conditions ◽  
Green Tea Extract ◽  
Viscosity Data ◽  
Skin Protection ◽  
Multiple Emulsion ◽  
Multiple Emulsions ◽  
Tea Extract

Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active (MeA) and without active (MeB). Both formulations showed comparable characteristics regarding various physicochemical characteristics in different storage conditions. Rheological analysis showed that formulations showed pseudo plastic behavior upon continuous shear stress. Results of in vitro and in vivo skin protection data have revealed that the active formulation has comparable skin protection effects to that of control formulation. It was presumed that stable multiple emulsions could be a promising choice for topical application of green tea but multiple emulsions presented in this study need improvement in the formula, concluded on the basis of pH, conductivity and apparent viscosity data.

scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

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