Effect of General Impact of Weak Low Frequency Vortex Magnetic Field on the System of Natural Antitumor Resistance of the Body

2019 ◽  
Vol 64 (6) ◽  
pp. 44-50
Author(s):  
Yu. Rybakov ◽  
V. Gukasov ◽  
Yu. Kozlov
Keyword(s):  
Breast Cancer ◽  
Magnetic Field ◽  
Functional Activity ◽  
Low Frequency ◽  
The Body ◽  
General Effect ◽  
Healthy Donors ◽  
The Impact ◽  
Observation Period

Purpose: Experimental study of the antitumor mechanism of increasing the functional activity of phagocytes with a general effect on the body of a weak low-frequency vortex magnetic field (VMP). Material and methods: The functional activity of phagocytes was assessed upon activation by the intensity of chemiluminescence (CHL) on a Biolumat instrument (model LB 9500, by Berthold, Germany). Samples for in vitro and in vivo experiments were prepared according to generally accepted protocols. The impact of the VMP (Vmax = 3.0 mTl, f = 100 Hz) was performed using a Magnitoturbotron magnetotherapeutic installation (developed by NPF Az). Results: It was found in vitro experiments that the exposure of a suspension of VMP cells stimulated an increase in CHL by 58 % relative to the control, while the main contribution to the intensity of the signal of the CHL was made by neutrophils. In the study of the blood CHL of mice with subcutaneously inoculated melanoma B-16, it was established that the value of the specific CHL in the experiment with VFM by the end of the observation period (day 17) increased sharply (3 times) relative to the beginning of the observation and to the control at the same observation period was 3.3 times more. Experiments with whole blood of healthy donors and patients with breast cancer showed that the CHL curves over time were biphasic in nature and had two maxima, but the phase ratio was different. At donors, the main luminescence developed by the 100th minute, and a maximum of 30–40 min was mild. In patients with breast cancer, the first maximum at 30–40 min was the main, the second maximum was weak and came later than that of donors. Experiments with the effects of VMP on the organism of healthy and patients with breast cancer of people showed an increase in the functional activity of neutrophils as a result of exposure to VMP, but in patients with breast cancer, the level of CHL was significantly (3 times) higher than of healthy donors. Based on the research, it was concluded that exposure to the VMP is able to form a priming neutrophil. Сonclusion: It is shown that the overall effect of weak low-frequency VFM increases the level of nonspecific resistance of the organism to the tumor process, which expands the possibilities of rehabilitation of patients, allows expanding the compensatory capabilities of the body and reduce the risk of disease.

THE DEGREE OF OXIDATIVE DAMAGE TO DNA IN VITRO AS A MOLECULAR PREDICTOR OF DISORDERS CAUSED BY EPIGENETIC AND EXOGENOUS FACTORS

2021 ◽  
Author(s):  
E. Tekutskaya ◽  
◽  
I. Raybova ◽  
Lyubov Ramazanovna Gusaruk ◽  
◽  
...  
Keyword(s):  
Magnetic Field ◽  
Reactive Oxygen Species ◽  
Oxidative Damage ◽  
Low Frequency ◽  
Oxygen Species ◽  
Healthy Donors ◽  
Exogenous Factors ◽  
Oxidative Damage To Dna ◽  
Molecular Predictor

In this work, we studied the mechanisms of oxidative damage to DNA molecules isolated from whole blood of healthy donors and patients with epigenetic disease (epidermolysis bullosa) when exposed to an alternating magnetic field of low frequency in vitro, associated with the formation of reactive oxygen species.

scholarly journals Evaluation of the adaptogenic propertries of the Quark product enriched with probiotics, polyphenols and vitamins

10.5219/1156 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 713-719 ◽  
Author(s):  
Zinaida Zobkova ◽  
Liliy Fedulova ◽  
Tatyana Fursova ◽  
Daria Zenina ◽  
Elena Kotenkova
Keyword(s):  
Magnetic Field ◽  
Blood Serum ◽  
Dairy Product ◽  
Low Frequency ◽  
Free Radical Oxidation ◽  
Variable Magnetic Field ◽  
The Body ◽  
Radical Oxidation ◽  
And Control ◽  
The Impact

The aim of the study is to evaluate protective properties of the quark product manufactured with transglutaminase and enriched with probiotics, oligomerous proanthocyanidines and vitamins; the biological experiment on the growing laboratory Wistar stock rats has been carried out. The rats of two from three groups subjected within 21 days to the effect of low-frequency weak variable magnetic field received in semi-synthetic diet composition extra experimental and control samples of the quark product. The index of feed intake and the rats’ body mass growth was registered within 32 days. At the end of the experiment blood serum biochemical index was evaluated. It was revealed that the animals consuming the experimental product substantially gained the mass before the effect (gain from the 1st up to 10th days made up 12%) as well as after effect (gain from 11th up to 32nd days – 10.3%); upon completion of the experiment the gains of these animals exceeded the gains of the rats consuming the control product by more than 28%. The experiment revealed the lipolipedemic and hypoglycemic effect of the experimental quark product that has been evidenced by the significant reduction of cholesterol (by more than 20%), glucose (up to 40%) in the rats’ blood serum. On administration of the experimental dairy product in the animals’ diet subjected to the impact of low-frequency weak magnetic field the effect of the broken balance recovery in antioxidant/pro-oxidant system was observed due to reduction of pro-oxidant load at the enzymatic as well as low molecular links of the antioxidant system. The identified antioxidant and adaptogenic effect of the developed dairy product promoting to reduce the intensity of free-radical oxidation at the impact of low-frequency electromagnetic field on the body make it possible recommend it in dietotherapy for correction of antioxidant/pro-oxidant status.

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

2018 ◽  
Vol 16 (2) ◽  
pp. 127-137
Author(s):  
Paula Sofia Coutinho Medeiros ◽  
Ana Lúcia Marques Batista de Carvalho ◽  
Cristina Ruano ◽  
Juan Carlos Otero ◽  
Maria Paula Matos Marques
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Breast Adenocarcinoma ◽  
Coumaric Acid ◽  
Human Breast ◽  
The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

scholarly journals 695 Oral delivery of a microbial extracellular vesicle induces potent anti-tumor immunity in mice

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A737-A737
Author(s):  
Loise Francisco-Anderson ◽  
Loise Francisco-Anderson ◽  
Mary Abdou ◽  
Michael Goldberg ◽  
Erin Troy ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Tumor Growth ◽  
Extracellular Vesicles ◽  
Tumor Immunity ◽  
Extracellular Vesicle ◽  
The Body ◽  
Checkpoint Inhibition ◽  
Small Intestinal ◽  
The Impact

BackgroundThe small intestinal axis (SINTAX) is a network of anatomic and functional connections between the small intestine and the rest of the body. It acts as an immunosurveillance system, integrating signals from the environment that affect physiological processes throughout the body. The impact of events in the gut in the control of tumor immunity is beginning to be appreciated. We have previously shown that an orally delivered single strain of commensal bacteria induces anti-tumor immunity preclinically via pattern recognition receptor-mediated activation of innate and adaptive immunity. Some bacteria produce extracellular vesicles (EVs) that share molecular content with the parent bacterium in a particle that is roughly 1/1000th the volume in a non-replicating form. We report here an orally-delivered and gut-restricted bacterial EV which potently attenuates tumor growth to a greater extent than whole bacteria or checkpoint inhibition.MethodsEDP1908 is a preparation of extracellular vesicles produced by a gram-stain negative strain of bacterium of the Oscillospiraceae family isolated from a human donor. EDP1908 was selected for its immunostimulatory profile in a screen of EVs from a range of distinct microbial strains. Its mechanism of action was determined by ex vivo analysis of the tumor microenvironment (TME) and by in vitro functional studies with murine and human cells.ResultsOral treatment of tumor-bearing mice with EDP1908 shows superior control of tumor growth compared to checkpoint inhibition (anti-PD-1) or an intact microbe. EDP1908 significantly increased the percentage of IFNγ and TNF producing CD8+ CTLs, NK cells, NKT cells and CD4+ cells in the tumor microenvironment (TME). EDP1908 also increased tumor-infiltrating dendritic cells (DC1 and DC2). Analysis of cytokines in the TME showed significant increases in IP-10 and IFNg production in mice treated with EDP1908, creating an environment conducive to the recruitment and activation of anti-tumor lymphocytes.ConclusionsThis is the first report of striking anti-tumor effects of an orally delivered microbial extracellular vesicle. These data point to oral EVs as a new class of immunotherapeutic drugs. They are particularly effective at harnessing the biology of the small intestinal axis, acting locally on host cells in the gut to control distal immune responses within the TME. EDP1908 is in preclinical development for the treatment of cancer.Ethics ApprovalPreclinical murine studies were conducted under the approval of the Avastus Preclinical Services’ Ethics Board. Human in vitro samples were attained by approval of the IntegReview Ethics Board; informed consent was obtained from all subjects.

scholarly journals CBR3 V244M is associated with LVEF reduction in breast cancer patients treated with doxorubicin

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jennifer K. Lang ◽  
Badri Karthikeyan ◽  
Adolfo Quiñones-Lombraña ◽  
Rachael Hageman Blair ◽  
Amy P. Early ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Care Center ◽  
Left Ventricular ◽  
The Body ◽  
Left Ventricular Ejection ◽  
Breast Cancer Patients ◽  
Ventricular Ejection Fraction ◽  
Echocardiographic Parameters ◽  
The Impact

Abstract Background The CBR3 V244M single nucleotide polymorphism has been linked to the risk of anthracycline-related cardiomyopathy in survivors of childhood cancer. There have been limited prospective studies examining the impact of CBR3 V244M on the risk for anthracycline-related cardiotoxicity in adult cohorts. Objectives This study evaluated the presence of associations between CBR3 V244M genotype status and changes in echocardiographic parameters in breast cancer patients undergoing doxorubicin treatment. Methods We recruited 155 patients with breast cancer receiving treatment with doxorubicin (DOX) at Roswell Park Comprehensive Care Center (Buffalo, NY) to a prospective single arm observational pharmacogenetic study. Patients were genotyped for the CBR3 V244M variant. 92 patients received an echocardiogram at baseline (t0 month) and at 6 months (t6 months) of follow up after DOX treatment. Apical two-chamber and four-chamber echocardiographic images were used to calculate volumes and left ventricular ejection fraction (LVEF) using Simpson’s biplane rule by investigators blinded to all patient data. Volumetric indices were evaluated by normalizing the cardiac volumes to the body surface area (BSA). Results Breast cancer patients with CBR3 GG and AG genotypes both experienced a statistically significant reduction in LVEF at 6 months following initiation of DOX treatment for breast cancer compared with their pre-DOX baseline study. Patients homozygous for the CBR3 V244M G allele (CBR3 V244) exhibited a further statistically significant decrease in LVEF at 6 months following DOX therapy in comparison with patients with heterozygous AG genotype. We found no differences in age, pre-existing cardiac diseases associated with myocardial injury, cumulative DOX dose, or concurrent use of cardioprotective medication between CBR3 genotype groups. Conclusions CBR3 V244M genotype status is associated with changes in echocardiographic parameters suggestive of early anthracycline-related cardiomyopathy in subjects undergoing chemotherapy for breast cancer.

scholarly journals Understanding the Impact of 2D and 3D Fibroblast Cultures on In Vitro Breast Cancer Models

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76373 ◽  
Author(s):  
Kyung Eun Sung ◽  
Xiaojing Su ◽  
Erwin Berthier ◽  
Carolyn Pehlke ◽  
Andreas Friedl ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fibroblast Cultures ◽  
Cancer Models ◽  
2D And 3D ◽  
The Impact

scholarly journals Novel Nanoparticle-Based Cancer Treatment, Effectively Inhibits Lung Metastases and Improves Survival in a Murine Breast Cancer Model

2021 ◽  
Vol 11 ◽  
Author(s):  
Sarah Kraus ◽  
Raz Khandadash ◽  
Raphael Hof ◽  
Abraham Nyska ◽  
Ekaterina Sigalov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Magnetic Field ◽  
Lung Metastases ◽  
Metastatic Cancer ◽  
Relative Size ◽  
Clinical Signs ◽  
Metastatic Breast ◽  
The Body ◽  
Alternating Magnetic Field ◽  
Therapeutic Modality

Sarah Nanoparticles (SaNPs) are unique multicore iron oxide-based nanoparticles, developed for the treatment of advanced cancer, following standard care, through the selective delivery of thermal energy to malignant cells upon exposure to an alternating magnetic field. For their therapeutic effect, SaNPs need to accumulate in the tumor. Since the potential accumulation and associated toxicity in normal tissues are an important risk consideration, biodistribution and toxicity were assessed in naïve BALB/c mice. Therapeutic efficacy and the effect on survival were investigated in the 4T1 murine model of metastatic breast cancer. Toxicity evaluation at various timepoints did not reveal any abnormal clinical signs, evidence of alterations in organ function, nor histopathologic adverse target organ toxicity, even after a follow up period of 25 weeks, confirming the safety of SaNP use. The biodistribution evaluation, following SaNP administration, indicated that SaNPs accumulate mainly in the liver and spleen. A comprehensive pharmacokinetics evaluation, demonstrated that the total percentage of SaNPs that accumulated in the blood and vital organs was ~78%, 46%, and 36% after 4, 13, and 25 weeks, respectively, suggesting a time-dependent clearance from the body. Efficacy studies in mice bearing 4T1 metastatic tumors revealed a 49.6% and 70% reduction in the number of lung metastases and their relative size, respectively, in treated vs. control mice, accompanied by a decrease in tumor cell viability in response to treatment. Moreover, SaNP treatment followed by alternating magnetic field exposure significantly improved the survival rate of treated mice compared to the controls. The median survival time was 29 ± 3.8 days in the treated group vs. 21.6 ± 4.9 days in the control, p-value 0.029. These assessments open new avenues for generating SaNPs and alternating magnetic field application as a potential novel therapeutic modality for metastatic cancer patients.

THE RISK OF DEVELOPING GYNECOLOGICAL CANCER IN WOMEN AFTER AN IN VITRO FERTILIZATION PROGRAM

2021 ◽  
pp. 7-13
Author(s):  
Allakhyarov D.Z. ◽  
Petrov Yu.A. ◽  
Palieva N.V.
Keyword(s):  
Breast Cancer ◽  
Ovarian Cancer ◽  
Endometrial Cancer ◽  
In Vitro Fertilization ◽  
Gynecological Cancer ◽  
The Body ◽  
Fertilization Program ◽  
Vitro Fertilization ◽  
Increased Risk

This article presents reviews of literature sources on the issue of assessing the risk of developing gynecological cancer in women after an in vitro fertilization program. Infertility and infertile marriages have now become quite a big problem of modern medicine. Against the background of the unfavorable demographic situation in the Russian Federation, this problem is becoming quite urgent. The main way to solve this situation is assisted reproductive technologies, among which the most common is in vitro fertilization. The in vitro fertilization program is accompanied by a hormonal ovulation stimulation procedure to obtain a female germ cell capable of fertilization. Against the background of the active use of the in vitro fertilization procedure, many patients had concerns related to the risk of developing gynecological cancer after the IVF procedure, which is due to the use of hormonal drugs to stimulate the ovaries. Also of concern is the fact that certain types of cancer, including ovarian cancer, endometrial cancer and breast cancer, are hormone-dependent. In this regard, multiple large-scale studies were conducted, which showed that the risk of developing gynecological cancer is really increased in patients after the in vitro fertilization program. In particular, breast cancer in women after the in vitro fertilization program is more common by 10%, and in women without a history of pregnancy and over the age of 40, it is more common by 31%. The increased risk may be due to age-related vulnerability to the effects of hormones or higher doses of hormones during the IVF procedure. Ovarian cancer and endometrial cancer are also more common in patients after IVF. According to the research results, it is suggested that it is not the IVF procedure itself that causes the development of cancer, but excessive hormonal load of the body, which leads to the launch of carcinogenesis.

A Mechanistic Motor-Clutch Model That Explains Cell Shape Dynamics to Cyclic Stretch

2022 ◽  
Author(s):  
Benjamin W. Scandling ◽  
Jia Gou ◽  
Jessica Thomas ◽  
Jacqueline Xuan ◽  
Chuan Xue ◽  
...  
Keyword(s):  
Computational Model ◽  
Computational Models ◽  
The Body ◽  
Cyclic Stretch ◽  
Substrate Stiffness ◽  
Cell Alignment ◽  
Cellular Mechanisms ◽  
Actin Bundles ◽  
The Impact

Many cells in the body experience cyclic mechanical loading, which can impact cellular processes and morphology. In vitro studies often report that cells reorient in response to cyclic stretch of their substrate. To explore cellular mechanisms involved in this reorientation, a computational model was developed by utilizing the previous computational models of the actin-myosin-integrin motor-clutch system developed by others. The computational model predicts that under most conditions, actin bundles align perpendicular to the direction of applied cyclic stretch, but under specific conditions, such as low substrate stiffness, actin bundles align parallel to the direction of stretch. The model also predicts that stretch frequency impacts the rate of reorientation, and that proper myosin function is critical in the reorientation response. These computational predictions are consistent with reports from the literature and new experimental results presented here. The model suggests that the impact of different stretching conditions (stretch type, amplitude, frequency, substrate stiffness, etc.) on the direction of cell alignment can largely be understood by considering their impact on cell-substrate detachment events, specifically whether detachment occurs during stretching or relaxing of the substrate.

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