Estimation in Vivo de Certains Paramètres de Laqualité de la Carcasse des Béliers de Race Barbarine Recevant des Régimes Supplémentés de Feuillage d’Artemesia Herba Alba ou de Rosmarinus Officinalis = Estimation in Vivo of Some Carcass Quality Parameters in Barbarine Breed Rams Receiving Diets Supplemented with Artemesia Herba Alba or Rosmarinus Officinalis Foliage = إضافة أوراق الشيح والإكليل إلى عليقة غذائية لدى أكباش سلالة البربري وتأثيرها على خصائص جودة الذبيحة على الجسم الحي

2017 ◽  
Vol 90 (90) ◽  
pp. 190-201
Author(s):  
Imen Baazaoui ◽  
Nabil Soumri ◽  
Zeineb Chouikh
Keyword(s):  
Quality Parameters ◽  
Rosmarinus Officinalis ◽  
Carcass Quality

In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits

2008 ◽  
Vol 116 (1) ◽  
pp. 64-73 ◽  
Author(s):  
Tülay Bakırel ◽  
Utku Bakırel ◽  
Oya Üstüner Keleş ◽  
Sinem Güneş Ülgen ◽  
Hasret Yardibi
Keyword(s):  
Antioxidant Activities ◽  
Rosmarinus Officinalis ◽  
Diabetic Rabbits ◽  
In Vivo Assessment

Relationships between carcass quality parameters and genetic types

Meat Science ◽  
2007 ◽  
Vol 77 (3) ◽  
pp. 397-404 ◽  
Author(s):  
M. Gispert ◽  
M. Font i Furnols ◽  
M. Gil ◽  
A. Velarde ◽  
A. Diestre ◽  
...  
Keyword(s):  
Quality Parameters ◽  
Carcass Quality ◽  
Genetic Types

68 GROWTH AND MARKET QUALITY OF PIGS BORN FROM MAGNETIC NANOPARTICLE-TREATED BOAR SPERMATOZOA

2017 ◽  
Vol 29 (1) ◽  
pp. 141 ◽  
Author(s):  
C. L. Durfey ◽  
S. Liao ◽  
D. Devost-Burnett ◽  
T. Dinh ◽  
M. Crenshaw ◽  
...  
Keyword(s):  
Meat Quality ◽  
Field Trapping ◽  
Poor Quality ◽  
Quality Parameters ◽  
Carcass Quality ◽  
Marbling Score ◽  
Boar Semen ◽  
High Concentrations ◽  
Student’S T ◽  
Area Percentage

Sperm ejaculates contain a heterogeneous population of nonviable and viable spermatozoal cells. Ejaculates with high concentrations of poor quality or damaged spermatozoa can greatly impair the overall fertility of males. Recently, a novel technique termed nanopurification has been developed (Feugang et al. 2015 IVF Reprod. Med. Genet. 3, 2) to noninvasively target and remove poor quality spermatozoa from boar semen. Such removal will enrich insemination doses with high quality spermatozoa to enhance fertility successes. However, effects associated with offspring born from nanopurified semen and possible meat quality assurance have yet to be extensively studied. The objective of this study was to measure the growth performance and market characteristics of pigs born from standard or nanopurified spermatozoa. Boar semen was obtained in insemination doses from a local stud and was mixed with (nanopurified) magnetic nanoparticles (iron-oxide) specifically designed to interact with acrosome-reacted and apoptotic spermatozoa. After incubation, mixed semen were placed under an electromagnetic field trapping moribund sperm to allow collection of intact and viable spermatozoa. Six gilts were bred with standard non-purified (control; n = 3) or nanopurified (n = 3) semen, with subsequent pregnancies leading to full-term birth of viable offspring. At weaning, pigs of equal sexes (5 male and 5 female) were randomly selected from control (n = 10) and nanopurified (n = 10) litters. Pigs were fed and measured until market weight, at which meat quality and carcass characteristics were assessed. Data (mean ± SEM) were analysed with Student’s t-test and SAS software (SAS Institute Inc., Cary, NC, USA). The threshold of significance was set as P < 0.05. Patterns of growth between groups were comparable up to market size (P > 0.05). Standard pork quality parameters (lean carcass weight, loin eye area, percentage of lean cuts, loin and ham colouring, etc.) revealed no significant differences between groups (P > 0.05). Dressing percentage was found higher in the nanopurified group compared with control, with a 1.5% increase (P < 0.05). Marbling score tended to be significantly higher in the nanopurified group (2.7 ± 0.15) when compared with the control (2.3 ± 0.15). Findings indicate that sperm nanopurification does not impair growth of offspring and could ultimately lead to a higher pork carcass quality. Additional research is being conducted to confirm current findings and identify further effects of nanopurification regarding offspring and carcass quality. This work was supported by USDA-ARS Grant #58–6402–3-018.

scholarly journals Inhibitors of zinc-dependent metalloproteases hinder sperm passage through the cumulus oophorus during porcine fertilization in vitro

Reproduction ◽  
2012 ◽  
Vol 144 (6) ◽  
pp. 687-697 ◽  
Author(s):  
J Beek ◽  
H Nauwynck ◽  
D Maes ◽  
A Van Soom
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Inhibitory Effect ◽  
Fertilization Rate ◽  
Quality Parameters ◽  
Fertilization In Vitro ◽  
Cumulus Oophorus ◽  
Sperm Penetration

In this study, we report for the first time on a possible contribution of metalloproteases in sperm passage through the cumulus matrix in pigs. The presence of 20 μM 1,10-phenanthroline (1,10-PHEN), inhibitor of zinc-dependent metalloproteases, strongly inhibited the degree of sperm penetration in cumulus-intact (CI), but not in cumulus-free (CF), porcine oocytes during IVF. The inhibitory effect of 1,10-PHEN was due to the chelation of metal ions as a non-chelating analog (1,7-PHEN) did not affect IVF rates. Furthermore, incubation with 1,10-PHEN did not affect sperm binding to the zona pellucida nor sperm motility, membrane integrity, or acrosomal status. These findings led to the assumption that 1,10-PHEN interacts with a sperm- or cumulus-derived metalloprotease. Metalloproteases are key players in physiological processes involving degradation or remodeling of extracellular matrix. In vivo, their proteolytic activity is regulated by tissue inhibitors of metalloproteases (TIMP1–TIMP4). We tested the effect of TIMP3 on fertilization parameters after porcine IVF. Similar to 1,10-PHEN, TIMP3 inhibited total fertilization rate of CI but not CF oocytes and did not influence sperm quality parameters. Although the inhibitory effect was stronger in CI oocytes, TIMP3 also reduced the degree of sperm penetration in CF oocytes, suggesting the involvement of a metalloprotease in a subsequent step during fertilization. In conclusion, our results indicate the involvement of TIMP3-sensitive, zinc-dependent metalloprotease activity in sperm passage through the cumulus oophorus in pigs. The results should provide the basis for further biochemical research toward the localization and identification of the metalloprotease involved.

scholarly journals The Therapeutic Potential of Rosemary (Rosmarinus officinalis) Diterpenes for Alzheimer’s Disease

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Solomon Habtemariam
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Therapeutic Potential ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Rosmarinus Officinalis ◽  
Brain Inflammation ◽  
Important Species

Rosemary (Rosmarinus officinalisL.) is one of the most economically important species of the family Lamiaceae. Native to the Mediterranean region, the plant is now widely distributed all over the world mainly due to its culinary, medicinal, and commercial uses including in the fragrance and food industries. Among the most important group of compounds isolated from the plant are the abietane-type phenolic diterpenes that account for most of the antioxidant and many pharmacological activities of the plant. Rosemary diterpenes have also been shown in recent years to inhibit neuronal cell death induced by a variety of agents bothin vitroandin vivo. The therapeutic potential of these compounds for Alzheimer’s disease (AD) is reviewed in this communication by giving special attention to the chemistry of the compounds along with the various pharmacological targets of the disease. The multifunctional nature of the compounds from the general antioxidant-mediated neuronal protection to other specific mechanisms including brain inflammation and amyloid beta (Aβ) formation, polymerisation, and pathologies is discussed.

scholarly journals Genotoxicity and mutagenicity of Rosmarinus officinalis (Labiatae) essential oil in mammalian cells in vivo

2010 ◽  
Vol 9 (4) ◽  
pp. 2120-2129 ◽  
Author(s):  
E.L. Maistro ◽  
S.F. Mota ◽  
E.B. Lima ◽  
B.M. Bernardes ◽  
F.C. Goulart
Keyword(s):  
Essential Oil ◽  
Mammalian Cells ◽  
Rosmarinus Officinalis

scholarly journals Fattening rabbits in mobile arks: effect of housing system on in vivo oxidative status and meat quality

2016 ◽  
Vol 24 (4) ◽  
pp. 303 ◽  
Author(s):  
S. Mattioli ◽  
M. Martino ◽  
S. Ruggeri ◽  
V. Roscini ◽  
L. Moscati ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Meat Quality ◽  
Essential Fatty Acids ◽  
Quality Parameters ◽  
Oxidative Status ◽  
Housing System ◽  
Longissimus Lumborum

<p>The aim of this trial was to study the effect of an alternative housing system on the oxidative status and meat quality of fattening rabbits. From May to June 2014, 60 rabbits of 35 d of age were reared in Mobile Arks (MA) placed on alfalfa grass and frequently moved for 40 d. To assess the health status of animals, blood samples were collected at slaughter in MA and in conventional cages (C). Meat quality parameters were also evaluated. Concerning the <em>in vivo </em>oxidative status, ark-reared rabbits showed higher thiobarbituric reactive substances values than C ones, probably for the higher motor activity due to the larger living area. The lipid percentage of <em>Longissimus lumborum </em>muscle was lower (1.22 <em>vs. </em>1.48%) in the ark group. There were no significant differences in the muscle pH, colour, water holding capacity and cooking loss. Given the higher intake of grass, rich in vitamins, carotenes, polyphenols and polyunsaturated fatty acids, the antioxidant content of meat was higher in ark-reared rabbits (7.42 <em>vs. </em>6.82 µg/g of retinol, 719.2 <em>vs. </em>683.3 ng/g of α-tocopherol, respectively). Even the fatty acid profile of MA rabbits reflected the higher intake of essential fatty acids from grass and the n-3 long chain polyunsaturated fatty acids (arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid) were almost doubled. Our study suggested that the fattening of rabbits in ark system could be a possible alternative system to improve the meat quality of rabbits.</p><p><strong><br /></strong></p>

scholarly journals Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

2015 ◽  
Vol 14 (1) ◽  
pp. 79
Author(s):  
Ririn Nurul Fauziah ◽  
Dinamella Wahjuningrum ◽  
, Sukenda ◽  
, Ranta
Keyword(s):  
Growth Rate ◽  
Streptococcus Agalactiae ◽  
Allium Sativum ◽  
Positive Control ◽  
Quality Parameters ◽  
Negative Control ◽  
Fish Pathogen ◽  
Phyllanthus Niruri

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">This study was aimed at determining potential of combination powder of garlic <em>Allium sativum</em>-shatterstone herb <em>Phyllanthus niruri</em> supplemented in feed against <em>S. agalactiae</em> infection in tilapia. Four concentrations of combination powder of <em>A. sativum</em>-<em>P. Niruri</em>; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in <em>in vitro</em> test. <em>In vivo</em> test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed (BM).  The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL <em>S. agalactiae</em> at concentration of 10<sup>5</sup> cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P&lt;0.05. In addition, <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed was also able to enhance the immune response by increasing phagocytic activity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Streptococcus agalactiae</em>, phytopharmacy, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, tilapia</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih <em>Allium sativum</em>-meniran <em>Phyllanthus niruri </em>dalam pakan terhadap pencegahan infeksi bakteri <em>S. agalactiae</em> pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam  menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji <em>in vitro</em>. Uji <em>in vivo</em> terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri <em>S. agalactiae</em> sebanyak 0,1 mL dengan kepadatan 10<sup>5 </sup>cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P&lt;0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Streptococcus agalactiae</em>, fitofarmaka, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, ikan nila</p><p> </p>

scholarly journals Multi-hole spherical CT scan method to characterize large quantities of bones in rats

2021 ◽  
Vol 30 (3) ◽  
pp. 182-90
Author(s):  
Neng Nenden Mulyaningsih ◽  
Ariadne Lakshmidevi Juwono ◽  
Djarwani Soeharso Soejoko ◽  
Dewi Apri Astuti
Keyword(s):  
Ct Scan ◽  
Hounsfield Unit ◽  
Spherical Model ◽  
Quality Parameters ◽  
In Vivo Studies ◽  
Ovariectomized Rats ◽  
Control Group ◽  
White Rats ◽  
Transmission Electron

BACKGROUND New therapeutic options are often explored in in vivo studies using animals like rats. Since rats are small, it is difficult to examine them in a computed tomography (CT) scan. This study aimed to introduce a multi-hole spherical model CT scan method as a new, fast, economical, and reliable method to characterize large quantities of rat bones at once in estimating the timing of osteoporosis in ovariectomized white rats. METHODS 50 female white rats (12 weeks old) were treated as the control group, and 40 rats of the same age were ovariectomized to establish the osteoporosis model. Sham rats were sacrificed at 13, 15, 17, 19, and 21 weeks old, while the ovariectomized rats were sacrificed at 15, 17, 19, and 21 weeks old. Afterward, tibia bones were removed, placed in the multi-hole spherical model, and characterized using a CT scan. Their characteristics were compared using a scanning electron microscope (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). RESULTS The Hounsfield unit scores resulted from the multi-hole spherical model CT scan method of tibia bones of rats were consistent with the percentage of the osteocyte cavities, canalicular diameters, and crystal size. The multi-hole spherical model CT scan method could produce 50 times more data than the SEM, TEM, or XRD. CONCLUSIONS Multi-hole spherical model CT scan was considered good and reliable in assessing bone quality parameters in rat samples simultaneously.

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