scholarly journals Antimicrobial Resistance and Genetic Characteristics of Salmonella Typhimurium Isolated from Horses in Hokkaido, Japan

2009 ◽  
Vol 71 (8) ◽  
pp. 1115-1119 ◽  
Author(s):  
Hidekazu NIWA ◽  
Toru ANZAI ◽  
Hidemasa IZUMIYA ◽  
Tomoko MORITA-ISHIHARA ◽  
Haruo WATANABE ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Genetic Characteristics

scholarly journals Plasmid Profiles and Antimicrobial Resistance in Salmonella typhimurium, S. enteritidis, and S. braenderup Isolates from Human Source

1987 ◽  
Vol 61 (2) ◽  
pp. 167-177
Author(s):  
Sankichi HORIUCHI ◽  
Nobuichi GOTO ◽  
Rintaro NAKAYA ◽  
Masayuki OGAWA ◽  
Takeshi ITO ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Plasmid Profiles ◽  
Human Source

scholarly journals Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 97
Author(s):  
Zunita Zakaria ◽  
Latiffah Hassan ◽  
Zawiyah Sharif ◽  
Norazah Ahmad ◽  
Rohaya Mohd Ali ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Multilocus Sequence Typing ◽  
Salmonella Enteritidis ◽  
Molecular Subtypes ◽  
Actin Binding ◽  
Diffusion Method ◽  
Genetic Characteristics ◽  
Meat Samples ◽  
Sequence Types

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.

scholarly journals Draft Genome Sequences of Multidrug-Resistant Campylobacter jejuni Strains Isolated from Chickens in Central China

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Yiluo Cheng ◽  
Wenting Zhang ◽  
Qin Lu ◽  
Guoyuan Wen ◽  
Qingping Luo ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Draft Genome ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Central China ◽  
Genome Sequences ◽  
Genetic Characteristics ◽  
Content Type

Campylobacter jejuni is a major foodborne pathogen that plays an important role in spreading drug resistance. We report the draft genome sequences of two multidrug-resistant C. jejuni isolates which contained similar mutations in the CmeR box. This will improve the understanding of C. jejuni antimicrobial resistance and genetic characteristics.

Salmonella Genomic Island 1 (SGI1) and genetic characteristics of animal and food isolates of Salmonella Typhimurium DT104 in Hungary

2008 ◽  
Vol 56 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Péter Fekete ◽  
Béla Nagy
Keyword(s):  
Salmonella Typhimurium ◽  
Gel Electrophoresis ◽  
Animal Species ◽  
Genomic Island ◽  
Phage Type ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Animal Origin ◽  
Genetic Characteristics ◽  
First Time

To study the genetic characteristics of DT104 strains of Salmonella Typhimurium and the prevalence of Salmonella Genomic Island (SGI1) in Hungary, 140 recent Salmonella strains of food and animal origin were examined. For the first time in Hungary, the SGI1 was found in 17 out of 59 S . Typhimurium isolates (all proven to be DT104 phage type). These 17 strains were then subtyped by pulsed-field gel electrophoresis (PFGE) into 6 pulsotypes which were less correlated with the geographic origin than with the animal species of origin.

Characterization of Antimicrobial-Resistant Phenotypes and Genotypes among Salmonella enterica Recovered from Pigs on Farms, from Transport Trucks, and from Pigs after Slaughter

2004 ◽  
Vol 67 (4) ◽  
pp. 698-705 ◽  
Author(s):  
WONDWOSSEN A. GEBREYES ◽  
PETER R. DAVIES ◽  
PAA-KOBINA TURKSON ◽  
W. E. MORGAN MORROW ◽  
JULIE A. FUNK ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Clavulanic Acid ◽  
Gel Electrophoresis ◽  
Rank Correlation ◽  
Pulsed Field Gel Electrophoresis ◽  
Mesenteric Lymph Nodes ◽  
Chloramphenicol Resistance ◽  
Pulsed Field ◽  
Amoxicillin Clavulanic Acid

The main objectives of this study were to determine antimicrobial resistance patterns among Salmonella serotypes and to evaluate the role of transport trucks in dissemination of antimicrobial-resistant strains of Salmonella. Salmonella from groups of nursery and finishing pigs on farms, from trucks, and from pigs after slaughter were compared using serotyping, patterns of antimicrobial resistance, and pulsed-field gel electrophoresis patterns. The five farms included in the study yielded 858 isolates representing 27 Salmonella serovars. The most common resistance observed (80% of all isolates) was to tetra-cycline; resistance to ampicillin (42%), chloramphenicol (31%), amoxicillin/clavulanic acid (30%), and piperacillin (31%) also were common. We found a correlation between serovar and antimicrobial resistance. High correlation was found between Salmonella Typhimurium var. Copenhagen and chloramphenicol resistance (Spearman rank correlation, ρ = 0.7). Multidrug resistance was observed primarily in Salmonella Typhimurium var. Copenhagen (94%) and Salmonella Typhimurium (93%) and was much less common in the other common serovars, including Salmonella Derby (7%) and Salmonella Heidelberg (8%). Of the 225 isolates exhibiting the most common pentaresistance pattern in this study, amoxicillin/clavulanic acid–ampicillin–chloramphenicol–piperacillin–tetracycline, 220 (98%) were Salmonella Typhimurium var. Copenhagen, and 86% of the isolates of this serovar had this pattern. Isolates from the trucks were similar, based on pulsed-field gel electrophoresis patterns, to those from the cecum and mesenteric lymph nodes of pigs on two of the farms, suggesting the probable infection of pigs during transport. Class I integrons were also common among various serovars.

Frequency and Antimicrobial Resistance of Salmonella Serotypes on Beef Carcasses at Small Abattoirs in Jalisco State, Mexico

2012 ◽  
Vol 75 (5) ◽  
pp. 867-873 ◽  
Author(s):  
JULIA A. PEREZ-MONTAÑO ◽  
DELIA GONZALEZ-AGUILAR ◽  
JEANNETTE BARBA ◽  
CARLOS PACHECO-GALLARDO ◽  
CARLOS A. CAMPOS-BRAVO ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Foodborne Pathogens ◽  
Multidrug Resistant ◽  
Control Measures ◽  
Wet Season ◽  
Beef Carcasses ◽  
Salmonella Serotypes ◽  
Group B ◽  
Inspection Service

The prevalence and antimicrobial resistance of Salmonella serotypes on beef carcasses from four small abattoirs in Jalisco State, Mexico, were investigated during a 10-month period. Following U.S. Department of Agriculture Food Safety and Inspection Service protocols, Salmonella was isolated from 78 (15.4%) beef carcasses (n = 505) after the final carcass water wash. Isolation frequency differed by establishment (P < 0.05) and was higher (P < 0.05) during the wet season (May through September) for all establishments. Thirteen Salmonella serotypes and four serogroups (partially serotyped isolates) were identified. The most prevalent were Salmonella enterica Give (24.4%), Salmonella Typhimurium (17.9%), and Salmonella Group B (14.1%). Antimicrobial susceptibility was tested against 11 drugs, and results indicated that 46.2% of the isolates were resistant to tetracycline, 42.3% were resistant to streptomycin, 23.1% were resistant to chloramphenicol, 21.8% were resistant to trimethoprim-sulfamethoxazole, and 19.2% were resistant to gentamicin. No resistance to ceftriaxone or ciprofloxacin was observed, and 33% of the isolates were resistant to three or more antimicrobials. Although Salmonella Give was the most prevalent serotype, 95% of the isolates of this serotype were susceptible to all antimicrobials tested. Antimicrobial resistance was more common in Salmonella Typhimurium, and 93% (13 of 14) of the isolates of this serotype were resistant to at least five antimicrobials. The frequency of multidrug-resistant Salmonella isolates differed among establishments (P < 0.05) and may be related to the origin of the cattle presented for harvesting. These findings highlight the need for control measures to reduce Salmonella prevalence on beef carcasses in small abattoirs in Mexico and for strategies to ensure the cautious use of antimicrobials in animal production to prevent and control the spread of antimicrobial-resistant foodborne pathogens.

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