scholarly journals An examination on various concentrations of mahkota dewa and meniran extract and liquid organic fertilizer in Centella asiatica shoot growth in vitro

2017 ◽  
Vol 14 (2) ◽  
pp. 47-55
Author(s):  
VENI SISKAYANTI ◽  
RETNA BANDRIYATI ARNIPUTRI ◽  
PRASWANTO PRASWANTO
Keyword(s):  
Shoot Growth ◽  
Organic Fertilizer ◽  
Centella Asiatica ◽  
Shoot Height ◽  
Media Type ◽  
Extract Concentration ◽  
Shoot Number ◽  
Media Types ◽  
The Brain

Siskayanti V, Arniputri RB, Praswanto. 2016. An examination on various concentrations of mahkota dewa and meniran extract and liquid organic fertilizer in Centella asiatica shoot growth in vitro. Biofarmasi 14: 47-55. Centella (Centella asiatica L) is one plant utilized as the traditional medicine because it has many benefits, one of which is to fulfill the brain requirement. The aims of this research was to know of media type, mahkota dewa and meniran extract, concentration of meniran, concentration of mahkota dewa, and concertation of liquid organic fertilizer what as well as shoot growth of Centella using tissue culture and filtering (2k design) that is expected it can be screened of the basic media types, organic material types (mahkota dewa and meniran extract), meniran concentration, mahkota dewa concentration and liquid organic fertilizer concentration. This research was taken place in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty of Sebelas Maret University, from November 2010 to June 2011. This research used 2k design with RAL environment. Factor 1: Media type ( ½ MS and MS); Factor 2: Concentration of liquid organic fertilizer (2 mL/L and 20 mL/L); Factor 3: Concentration of meniran 5 mL/L and 25 mL/L); and Factor 4: Concentration of mahkota dewa (5 mL/L and 25 mL/L). Thus, there is 24 = 16 treatment combinations, each of which is repeated three times. The variables observed are shoot emerging duration, shoot number, shoot height, and leaves number. The data were analyzed descriptively. The result of research showed that basic media MS, that basic media MS, POC 20 mL/L, and meniran extract 5 mL/L provided the best explants growth of Centella through in vitro.

The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro

2012 ◽  
Vol 10 (2) ◽  
pp. 54-60
Author(s):  
IRMA PUTRI HAYANTI ◽  
RETNA BANDRIYATI AMIPUTRI ◽  
PRASWANTO PRASWANTO
Keyword(s):  
Shoot Growth ◽  
Organic Fertilizer ◽  
Centella Asiatica ◽  
Culture Method ◽  
Shoot Formation ◽  
Randomized Design ◽  
Leaf Formation ◽  
Medicinal Crop ◽  
Completely Randomized Design

Hayanti IP, Amiputri RB, Praswanto. 2012. The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro. Biofarmasi 10: 54-60. Gotu kola (Centella asiatica L.) is a medicinal crop, needed by local industry of pharmacy. Gotu kola in Indonesia is not enough to supply in market requisites. Tissue culture method was used to multiply gotu kola with a combination of medium from liquid organic fertilizer and pearl grass extract. The purposes of the research were to determine the effect of liquid organic fertilizer and pearl grass extract on the shoot growth of gotu kola in vitro, and to obtain the optimal concentration of liquid organic fertilizer and pearl grass extract combination on the shoot formation of gotu kola in vitro. The research was conducted in November 2010 to June 2011 in Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, Surakarta. The experimental design used was a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was liquid organic fertilizer concentrations, i.e. 0 mL/L, 2 mL/L, 4 mL/L and 8 mL/L. The second factor was pearl grass extract, i.e. 0 mL/L, 3 mL/L, 6 mL/L and 12 mL/L. Variables observed were the percentage of shoot formation, the time of shoot formation, the number of shoot, the length of shoot, the percentage of leaf formation, the time of leaf formation and the number of leaf. The result of the research showed that not all of treatments provided shoot formation. The combination of liquid organic fertilizer concentrations 0 mL/L and pearl grass extract 12 mL/L showed the best results, in which the average of length of shoot was 2.16 cm, the number of shoot was 8 and the number of leaf was 8.

scholarly journals INTERAKSI BAP DAN NAA TERHADAP PERTUMBUHAN EKSPLAN ANGGREK VANDA SECARA IN-VITRO

2017 ◽  
Vol 29 (1) ◽  
pp. 1-8
Author(s):  
Selvia Sutriana ◽  
Hasan Basri Jumin ◽  
Mardaleni Mardaleni
Keyword(s):  
Shoot Growth ◽  
Shoot Height ◽  
Significance Level ◽  
Abnormal Embryo ◽  
Randomized Design ◽  
Shoot Number ◽  
Completely Randomized Design ◽  
Growing Shoot

This research aims to obtain the combination of BAP and NAA concentration which give the best effect on explant growth of Vanda orchid in-vitro. The research was conducted at the biotechnology laboratory Faculty of Agriculture Riau Islamic University Pekanbaru from August to December 2013. The completely randomized design with two factorials (BAP and NAA) was used. The observed parameters included percentage of shoot growing, shoot age, shoot height, shoot number and abnormal embryo somatic. Data were analysed using statistics and BNJ Test at the 5% significance level. The result showed that,interactionally,BAP and NAA applications had a significant effect on height plant with the best concentration without BAP and 1.0 ppm NAA.  Individually, the application of BAP effected significantly on percentage of shoot growing, shoot growth, shoot height and shoot number with the best concentration without BAP and 0.1 BAP. Furthermore, the application of NAA influenced significantly on percentage of shoot growing, shoot age, shoot height and shoot number with the best concentration of 1.0 ppm NNA

Growth and Development of Field Horsetail (Equisetum arvenseL.)

Weed Science ◽  
1986 ◽  
Vol 34 (2) ◽  
pp. 271-275 ◽  
Author(s):  
George Marshall
Keyword(s):  
Growth And Development ◽  
Dry Matter ◽  
Shoot Growth ◽  
Equisetum Arvense ◽  
Shoot Height ◽  
Potted Plants ◽  
Shoot Number ◽  
Herbicide Treatments ◽  
Shoot Growth Rate ◽  
Rhizome System

The growth and development of field horsetail (Equisetum arvenseL. # EQUAR) was measured outdoors using potted plants grown from one-node rhizome fragments. Shoot growth rate increased slowly after planting in March, accelerating to a maximum during July with maximas for shoot height during August and shoot number in September. Rhizome system growth rates initially followed a similar pattern to that of the shoots achieving a maximum during July. Dry matter accumulated in the rhizomes until October and declined thereafter throughout the winter. Tubers initiated during August grew in size and number until November, well beyond the period of active shoot growth and onset of senescence. The characteristics of the growth and development of field horsetail are discussed in relation to achieving weed control via cultural and herbicide treatments.

scholarly journals Inhibition of cPLA2 and sPLA2 Activities in Primary Cultures of Rat Cortical Neurons by Centella asiatica Water Extract

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Patrícia P. Defillipo ◽  
André H. Raposo ◽  
Alessandra G. Fedoce ◽  
Aline S. Ferreira ◽  
Hudson C. Polonini ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Leaf Extract ◽  
Primary Cultures ◽  
Water Extract ◽  
Centella Asiatica ◽  
Long Time ◽  
Rat Cortical Neurons ◽  
Low Toxicity ◽  
The Brain

Leaf extract of Centella asiatica has been used as an alternative medicine for memory improvement in the Indian Ayurvedic system of medicine for a long time. Although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of Alzheimer's disease, the molecular mechanism of C. asiatica on neuroprotection still remains unexplained. In this study, we investigated the effects of C. asiatica water extract on activity of subtypes of phospholipase A2 (PLA2) in primary cultures of rat cortical neurons and quantified by HPLC a possible molecule responsible for the activity. The cPLA2 and sPLA2 activities were inhibited in vitro by asiaticoside present in the water extract of C. asiatica. This extract may be a candidate for the treatment of neurodegenerative processes because of its pharmacological activity in the brain and its low toxicity, as attested by its long popular use as a natural product.

scholarly journals Impact of Phytomediated Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response of In Vitro Raised Shoots of Ochradenus arabicus

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Fahad Al-Qurainy ◽  
Salim Khan ◽  
Saleh Alansi ◽  
Mohammad Nadeem ◽  
Aref Alshameri ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Shoot Growth ◽  
System Response ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Shoot Number ◽  
High Concentrations

Biogenic nanoparticles have potential roles in the growth and development of plants and animals as they are ecofriendly and free of chemical contaminants. In this study, we assessed the effects of phytomediated zinc oxide nanoparticles (ZnONPs) on shoot growth, biochemical markers, and antioxidant system response in Ochradenus arabicus, which is a medicinal plant. The shoot length and fresh and dry weights were found to be higher in groups with 5 and 10 mg/L ZnONPs than in the control. At high concentrations of ZnONPs (50, 100, and 300 mg/L), biomass was decreased in a concentration-dependent manner. The shoot number was observed to be highest at 50 mg/L among all applied concentrations of ZnONPs. The levels of the stress markers proline and TBARS were found to be higher in shoots treated with 100 and 300 mg/L ZnONPs than in the control as well as NP-treated shoots. The levels of antioxidant enzymes were significantly increased at high concentrations of nanoparticles compared with the control. Thus, synthesized phytomediated ZnONPs from shoots of O. arabicus and their application to the same organ of O. arabicus in vitro were found to be effective as a low concentration of nanoparticles promoted shoot growth, resulting in high biomass accumulation. Thus, using green nanotechnology, such endemic plants could be conserved in vitro and multiple shoots could be produced by reducing the phytohormone concentration for multiple uses, such as the production of potential secondary metabolites.

scholarly journals Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

2018 ◽  
Vol 4 (2) ◽  
pp. 93
Author(s):  
Douglas Bertoncelli ◽  
Guilherme Alves ◽  
Gustavo Freiria ◽  
Felipe Furlan ◽  
Helio Neto ◽  
...  
Keyword(s):  
Root Length ◽  
Iron Concentration ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Nutritional Requirement ◽  
Initial Development ◽  
Shoot Height ◽  
Shoot Number ◽  
Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

scholarly journals Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas

2021 ◽  
Vol 4 (1) ◽  
pp. 82-92
Author(s):  
Rustikawati Rustikawati ◽  
Catur Herison ◽  
Entang Inoriah ◽  
Vera Dwisari
Keyword(s):  
Shoot Growth ◽  
Shoot Formation ◽  
Experimental Unit ◽  
Shoot Height ◽  
Weight Percentage ◽  
Randomized Design ◽  
Significant Difference ◽  
Long Time ◽  
Planting Materials

Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.

scholarly journals Organic fertilizer on the in vitro cultivation of the Cattleya labiata orchid

2017 ◽  
Vol 2 (2) ◽  
pp. 62
Author(s):  
Rodrigo Thibes Hoshino ◽  
Ronan Carlos Colombo ◽  
Ana Paula Zandoná ◽  
Guilherme Augusto Cito Alves ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Organic Fertilizer ◽  
Dry Mass ◽  
In Vitro Cultivation ◽  
Shoot Height ◽  
Organic Products ◽  
Number Of Leaves

Propagation of in vitro plants through other culture media rather than the traditional ones has been widely researched, with satisfactory results. However, to increase the effectiveness of these media, the addition of organic products has been presented satisfactory results. Therefore, the objective of this work was to evaluate the influence of the FishFértil® organic fertilizer on the in vitro cultivation of the Brazilian orchid Cattleya labiata. Treatments included FishFértil® fertilizer concentrations of 0; 1; 2; 3; 4; 5 and 6 mL L-1 in a simplified culture medium. At 180 days, shoot height, number of leaves, leaf area, number of roots, root average length, shoot and root dry mass and the shoot: root ratio were evaluated. The experimental design was entirely randomized, with 10 replications, each one containing 10 plantules. Data were submitted to an analysis of variance and regression analysis, at 5% of significance. The FishFértil® organic fertilizer at the concentration of 6 mL L-1 promoted better plantule growth of Cattleya labiata, subcultivated in vitro, in a simplified culture medium.

scholarly journals Pertumbuhan Tunas Pisang Barangan (Musa Acuminata Colla.) akibat Pemberian Benzyl Amino Purin dan Arang Aktif secara In Vitro.

2019 ◽  
Vol 4 (1) ◽  
pp. 73-90
Author(s):  
Mulia Saputri ◽  
Marai Rahmawati ◽  
Elly Kesumawati
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Shoot Growth ◽  
Growth Time ◽  
Shoot Height ◽  
High Productivity ◽  
Horticultural Crops ◽  
Randomized Design ◽  
And Control

Pertumbuhan Tunas Pisang Barangan Akibat Pemberian Benzyl AminoPurin dan Arang Aktif secara In Vitro Accretion of Barangan Banana Shoot Effect of  (BAP)  and Activated Charcoal Explant by In VitroAbstrak. Banana as a superior product of horticultural crops, has not achieved high productivity and has several obstacles in its multiplication. Tissue culture is one solution to overcome this problem. This study aims to determine the composition of PGRBenzil Amino Purine (BAP) and activated charcoal that are appropriate in the multiplication of barangan banana shoots. This research was conducted at the Tissue Culture Laboratory Faculty of Agriculture Syiah Kuala University, Darussalam Banda Aceh. The design used was a Completely Randomized Design (CRD) with two treatment factors. The first factor is BAP concentration consisting of 3 levels, namely 4 mg/L, 6 mg /L, and 8 mg/L. The second factor is activated charcoal concentration consisting of 3 levels, namely control, 1 g/L and 2 g/L. In this study, from 9 treatment combinations, only 4 treatment combinations were not contaminated. Of the 4 treatment combinations the combination of BAP concentration of 6 mg/L and control (without activated charcoal) showed the fastest shoot growth time of 29 days after multiplication, the most shoot growth was 6 shoots and the average shoot height was 15.9 mm

scholarly journals In Vitro Shoot Proliferation of Apple Rootstocks ‘B.9’, ‘G.30’, and ‘G.41’ Grown under Red and Blue Light

HortScience ◽  
2015 ◽  
Vol 50 (3) ◽  
pp. 430-433 ◽  
Author(s):  
Fang Geng ◽  
Renae Moran ◽  
Michael Day ◽  
William Halteman ◽  
Donglin Zhang
Keyword(s):  
Blue Light ◽  
White Light ◽  
Shoot Growth ◽  
Light Quality ◽  
Red Light ◽  
Shoot Proliferation ◽  
Apple Rootstocks ◽  
Single Node ◽  
Shoot Number

The influence of red and blue light wavelengths was tested to improve the initial in vitro multiplication of apple (Malus × domestica) rootstock cultivars Budagovsky 9 (B.9), Geneva 30 (G.30), and Geneva 41 (G.41). Single-node segments were established in semisolid Murashige and Skoog media and then transferred to proliferation media and cultured 40 days under white, red, or blue light irradiance. In a second experiment, G.30 was cultured under red, blue, or white light with and without gibberellic acid (GA3). The three rootstocks responded similarly under white light in terms of shoot number, length of the longest shoot, and the number of elongated shoots. Red light increased the number of shoots, length of the longest shoot, and the number of elongated shoots of B.9 and G.30 when compared with white or blue light. Red light increased the number of elongated B.9 and G.30 shoots to five per explant compared with one per explant under white light. In contrast, shoot growth of G.41 showed no difference under the three light quality treatments, and the number of elongated shoots per explant was less than one. When compared with an absence of GA3, a concentration of GA3 at 0.5 mg·L−1 promoted in vitro shoot growth of G.30 under red and blue light.

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