Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

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Cellulase enzyme activity of the bacteria isolated from mangrove ecosystem in Aceh Besar and Banda Aceh

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/951/1/012113 ◽

2022 ◽

Vol 951 (1) ◽

pp. 012113

Author(s):

I Dewiyanti ◽

D Darmawi ◽

Z A Muchlisin ◽

T Z Helmi ◽

I I Arisa ◽

...

Keyword(s):

Enzyme Activity ◽

Congo Red ◽

Cellulase Activity ◽

Mangrove Ecosystem ◽

Microbiology Laboratory ◽

Cellulolytic Bacteria ◽

Clear Zone ◽

Cellulase Enzyme ◽

Mangrove Soil ◽

Quarantine Station

Abstract Cellulolytic bacteria is one of the beneficial bacteria that can found in mangrove ecosystem. The purposes of study were to analyse the cellulolytic index, and to analyse the cellulase activity of bacteria isolated from soil mangrove. Qualitatively, assessment of cellulase activity were carried out at the Microbiology laboratory of Fish Quarantine Station, Quality Control and Safety of Fishery Products (SKIPM) Aceh, while quantitatively was observed in microbiology laboratory, Biology Department, IPB. Assessment of qualitative cellulase activity is performed by growing the selected pure isolate on 1% CMC medium then spilled 1% congo red to test its cellulolytic potential. Cellulolytic potential was determined by clear zone performed around the colony after congo red flooded. The quantitative cellulase enzyme activity test carried out by DNS method tested on one selective isolate. There were 21 from 39 isolates showed a clear zone isolated from mangrove soil. The cellulolytic index (CI) obtained ranged from 0.07 to 0.80 classified as low cellulolytic index criteria. The cellulolytic index was higher in bacteria isolated from mangrove rehabilitated than mangrove unrehabilitated. The highest cellulase activity and specific cellulase activity of BTMD32 was at 48 hours with the value were 0.0012 U/ml, 0.077 U/mg, respectively. The result concluded that the bacteria cellulolytic isolated from mangrove soil had low cellulolytic index, low cellulase activity, and low specific cellulase activity.

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Produksi Enzim Selulase Kasar dari Isolat Bakteri B2S8 menggunakan Substrat Brangkasan Jagung dengan Perlakuan Konsentrasi Inokulum dan Komposisi Media yang berbeda

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI ◽

10.24843/jrma.2020.v08.i02.p11 ◽

2020 ◽

Vol 8 (2) ◽

pp. 267

Author(s):

Nursatria Purba ◽

Ida Bagus Wayan Gunam ◽

I Made Mahaputra Wijaya

Keyword(s):

Enzyme Activity ◽

Protein Content ◽

Corn Stover ◽

Bacterial Isolate ◽

Specific Activity ◽

Block Design ◽

Cellulolytic Bacteria ◽

Endoglucanase Activity ◽

Cellulase Enzyme ◽

The Media

The purpose of this study was determined the media and concentration of cellulolytic bacterial isolates to produce high cellulase enzyme activity. Production of crude cellulase enzyme in media and concentration of different bacterial isolate used a factorial Randomized Block Design (RBD) which consist of two factors. The first factor was the media production of different cellulase enzyme consisting of 3 levels, namely media 1, 2 and 3. The second factor was the concentration of bacterial isolate consisting of 5 levels namely 1, 2, 3, 4 and 5%. This study used a B2S8 cellulolytic bacterial isolate that has the highest value of cellulase enzyme activity and the highest degradation rate of cellulose in previous studied and determined the ability of exoglucanase enzyme activity, endoglucanase enzyme and dissolved protein content produced from cellulolytic bacterial isolate. This study used Carboxymethyl Cellulose (CMC) for enzyme activity test and 1% corn stover as a substrate on the media to produce crude cellulase enzyme. The result showed that the highest cellulase enzyme activity in the third media and 5% cellulolytic bacterial inoculum concentration resulted in endoglucanase activity of 0.0332 IU/mL, exoglucanases enzyme activity of 0.0060 IU/mL, dissolved protein content in the amount of 0.5670 mg/mL, the specific endoglucanase activity of 0.0807 IU/mg and the specific activity of exoglucanase of 0.0123 IU/mg. Keywords: Cellulolytic bacteria, Cellulase enzymes, Enzyme activity, Corn stover

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Produksi Enzim Selulase olehAspergillus niger pada Ampas Sagu

Jurnal Natur Indonesia ◽

10.31258/jnat.16.1.1-9 ◽

2015 ◽

Vol 16 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Nora Idiawati ◽

Elliska Murni Harfinda ◽

Lucy Arianie

Keyword(s):

Enzyme Activity ◽

Aspergillus Niger ◽

Moisture Content ◽

Cellulase Enzymes ◽

Fermentation Time ◽

Cellulase Enzyme ◽

Sago Waste ◽

Solid Fermentation

Production of cellulase by Aspergillus niger was carried out by growing the cultureson sago waste. Sago waste containscellulose that has not been used optimally. Cellulose is a polysaccharide consisting of glucose monomers linked by β-1,4-glycosides bonds. Glycoside bonds in cellulose can be enzymatically hydrolyzed into glucose with cellulase enzymes. Solid fermentation used to produce cellulase on sago waste as substrate was influenced by pH (3 to 6), moisture content(40% to 85%), and fermentation time (4 to 10 days). Products of the cellulase enzyme activity was measured by phenolsulfuricacid method. The results showed that the highest cellulase enzyme activity was 0.172 U/mL obtained at 85%moisture content, pH 5, and 8 days of fermentation time.

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Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v8i3.7263 ◽

2016 ◽

Vol 8 (3) ◽

pp. 386

Author(s):

Wijanarka Wijanarka ◽

Endang Kusdiyantini ◽

Sarjana Parman

Keyword(s):

Enzyme Activity ◽

Digestive Tract ◽

Generation Time ◽

Cellulase Activity ◽

Biology Education ◽

Activity Measurement ◽

Bacterial Isolates ◽

Achatina Fulica ◽

Cellulolytic Bacteria ◽

Activity Measurements

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.

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The Effect of Addition Mn2+ Metal Ions and Incubation Time to Bacillus cereus Cellulase Enzyme Activity from Endophytic Bacteria of Curcuma Rhizome (Curcuma zanthorrizha Roxb.)

el–Hayah ◽

10.18860/elha.v8i2.12463 ◽

2021 ◽

Vol 8 (2) ◽

pp. 62-69

Author(s):

Cahyaning Sulistyantini ◽

Ulfah Utami

Keyword(s):

Enzyme Activity ◽

Metal Ions ◽

Bacillus Cereus ◽

Incubation Time ◽

Endophytic Bacteria ◽

Cellulase Enzymes ◽

Cellulase Enzyme ◽

Randomized Design ◽

Completely Randomized Design ◽

Range Test

Cellulase is one of the most widely used enzymes in the industrial world and wastes decomposition process. Bacillus cereus is one of the bacteria that can produce cellulase enzymes that can hydrolyze cellulose to glucose. The addition of cofactors and incubation time can help determine the optimum conditions needed by cellulase enzymes to work optimally. This study aims to determine the effect of adding Mn2+ metal ions and incubation time to the activity of cellulase enzymes from Bacillus cereus endophytic bacteria. This research is experimentally used a Completely Randomized Design (CRD) factorial design with two factor treatments and 3 times repetitions. The first factor is variation of addition Mn2+ metal ions which are 5 mM, 10 mM, and 15 mM, the second is variation of incubation time which are 1 hour, 2 hours, 3 hours, 4 hours, and 5 hours. The data were analyzed using Analysis Of Variance (ANOVA) and if the data significantly affected the parameter, then it would be continued by Duncan Multiple Range Test (DMRT) with the fault level of 5%. The result showed that the interaction of addition Mn2+ metal ions and incubation time affected the cellulase enzyme activity of Bacillus cereus from endophytic bacteria. The highest cellulase enzyme activity obtained from interaction addition Mn2+ metal 10 mM and incubation time 3 hours with an activity 0,335 U/mL, while the lowest cellulase enzyme activity obtained from interaction addition Mn2+ metal 15 mM and incubation time 5 hours with an activity of 0,073 U/mL

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OPTIMASI SUHU DAN pH TERHADAP AKTIVITAS ENZIM ENDOGLUKANASE MENGGUNAKAN RESPONSE SURFACE METHODOLOGY (RSM)

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI ◽

10.24843/jrma.2019.v07.i02.p08 ◽

2019 ◽

Vol 7 (2) ◽

pp. 243

Author(s):

Ambar Kusumaningrum ◽

Ida Bagus Wayan Gunam ◽

I Made Mahaputra Wijaya

Keyword(s):

Response Surface Methodology ◽

Enzyme Activity ◽

Response Surface ◽

Bacterial Isolate ◽

Cellulose Degradation ◽

Growth Media ◽

Cellulolytic Bacteria ◽

Cellulase Enzyme ◽

Test Optimization ◽

Central Composite

This study aims to determine the optimal temperature and pH for the growth of cellulolytic bacterial isolates to produce high cellulase enzyme activity. This study used one potential cellulolytic bacterial isolate B2S8 which had the highest cellulose degradation value and highest cellulase enzyme activity in previous studies. Carboxymethyl Cellulose (CMC) was used as a substrate on growth media and enzyme activity test. Optimization of temperature and pH on cellulase enzyme activity was done by Response Surface Methodology (RSM) by Central Composite Design (CCD). The results using Response Surface Methodology (RSM) showed that the highest endoglucanase enzyme activity was at 36.9 ºC and pH 6.9 was equal to 0.0269 IU/mL. Keywords: cellulolytic bacteria, endoglucanase enzyme, activity, Response Surface Methodology.

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PEMANFAATAN AMPAS SAGU (Metroxylon Sagu Rottboel.) HASIL FERMENTASI TRICHODERMA HARZIANUM RIFAI DAN PENAMBAHAN MIKROFLORA ALAMI PENCERNAAN AYAM BROILER DALAM PEMBUATAN PAKAN AYAM KONSENTRAT BERPROBIOTIK

Jurnal BiBieT ◽

10.22216/jbbt.v2i2.2923 ◽

2017 ◽

Vol 2 (2) ◽

pp. 68

Author(s):

Sri Indrayati ◽

Yulia M Nur ◽

Periadnadi Periadnadi ◽

Nurmiati Nurmiati

Keyword(s):

Enzyme Activity ◽

Trichoderma Harzianum ◽

Broiler Chickens ◽

Total Population ◽

Ph Value ◽

Cellulase Activity ◽

Poultry Feed ◽

Cellulolytic Bacteria ◽

Sun Drying ◽

Metroxylon Sagu

Konversi Ampas sagu menggunakan kapangTrichoderma harzianum merupakan salah satu penerapan teknologi alami untuk menghasilkan bahan pakan alternatif untuk ayam broiler. Untuk meningkatkan efisiensi kecernaannya juga dapat ditambahkan probiotik yang diisolasi dari mikroflora usus ayam broiler. Tujuan penelitian untuk melihat kemampuan enzimatis (selulase dan amilase) dari Kapang Trichoderma harzianum dalam menfermentasi ampas sagu menjadi pakan ayam broiler dan selanjutnya untuk mengetahui proses pengeringan pakan konsentrat berprobiotik yang tepat. Penelitian ini telah dilaksanakan pada bulan Desember 2016 sampai Agustus tahun 2017 di Laboratorium Kopertis X, Padang. Penelitian ini menggunakan metoda eksperimen dengan tiga ulangan dan dua tahap penelitian. Tahap pertama adalah fermentasi ampas sagu menggunakan koji dari kapang Trichoderma harzianum. Sedangkan tahap kedua adalah pembuatan pakan konsetrat dengan penambahan isolat probiotik dari pencernaan ayam broiler dengan pengeringan matahari, pengeringan angin, dan pengeringan suhu 50oC. Hasil penelitian menunjukkan kemampuan enzimatis kapang Trichoderma harzianum dalam memfermentasi sagu adalah aktivitas amilase 1,420Unit/g, aktivitas selulase 0,427 Unit/g, kadar glukosa 36,150 μg/g, dan nilai PH 4,20. Sedangkan proses pengeringan yang paling baik untuk pengembangan pakan konsentrat probiotik dari ampas sagu adalah pengeringan matahari dengan total populasi bakteri amilolitik 97x109 cfu/g, total populasi bakteri selulolitik 89x107 cfu/g, aktivitas enzim amilase 2,012 unit/g, aktivitas enzim selulase 1,870 unit/g, kadar glukosa105,311 μg/g, dan nilai pH 6,34Conversion of sago dregs (Metroxylon sagu) using Trichoderma harzianum mold is one of the applications of natural technology to produce alternative feed material for broiler chicken. To increase the efficiency of digestibility can also be added probiotics isolated from the intestinal microflora of broiler chickens. The objective of the study was to look at enzymatic capability (cellulase and amylase) from Trichoderma harzianum mold in fermenting sago dregs into broiler feed and then to know the process of drying the right concentrates of poultry feed. This research was conducted in December 2016 until August 2017 at Kopertis X Laboratory, Padang. This research used experimental method with three replications and two research phases. The first stage is the fermentation of sago dregs(Metroxylon sagu) using koji from Trichoderma harzianum mold. While the second stage is the manufacture of concrete feed with the addition of probiotic isolates from the digestion of broiler chickens with sun drying, wind draining, and 50oC drying temperature. The results showed that enzymatic capability of Trichoderma harzianum in fermentation of sago dregs was 1.420 Unit/g amylase activity, cellulase activity 0,427 Unit/g, glucose level 36,150 μg/g, and PH value 4,20. While the best drying process for the development of probiotic concentrate feed from sago dregs is sun drying with total population of amylolitic bacteria 97x109 cfu/g, total population of cellulolytic bacteria 89x107 cfu/g, amylase enzyme activity 2,012 unit/g, cellulase enzyme activity 1,870 units/g, glucose 105,311 μg/g, and pH value of 6.34

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Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

The Open Microbiology Journal ◽

10.2174/1874285801812010154 ◽

2018 ◽

Vol 12 (1) ◽

pp. 154-162 ◽

Cited By ~ 3

Author(s):

Zaw K. Latt ◽

San S. Yu ◽

Ei P. Kyaw ◽

Tin M. Lynn ◽

May T. Nwe ◽

...

Keyword(s):

Enzyme Activity ◽

Screening Method ◽

Carbon Sources ◽

Cellulase Activity ◽

Agricultural Residues ◽

Cellulolytic Activity ◽

Nitrogen Fixing ◽

Cellulase Enzyme ◽

Ammonium Accumulation ◽

Test Kit

Introduction:Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar.Methods & Materials:The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS).Results & Discussion:The isolatedA. agilisgrew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity ofA. agiliswas highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity ofA. agilisfor cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity ofA. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust.Conclusion:So, the isolatedA. agilishas high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues.

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Exploring cellulolytic microorganisms from coffee industry by-products and their enzyme properties

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/924/1/012075 ◽

2021 ◽

Vol 924 (1) ◽

pp. 012075

Author(s):

A A Brahmanti ◽

E Martati ◽

A K Wardani

Keyword(s):

Enzyme Activity ◽

Specific Activity ◽

Cellulase Activity ◽

Cellulase Production ◽

Zone Formation ◽

Cellulolytic Microorganisms ◽

Activity Measurements ◽

Congo Red Staining ◽

Immense Potential ◽

By Products

Abstract Cellulolytic microorganism has immense potential due to their cellulase production, enzyme complexity and widespread habitat of life. This study was conducted to obtain microbial cellulase with vast industrial applicability from the coffee industry by-product in East Java, Indonesia. Fifty-four isolates with significant clear zone formation were obtained by Congo red staining in CMC agar plates. Eighteen bacteria, two yeasts and two moulds with high cellulolytic index were subjected to protein content determination as well as reducing sugar analysis in various conditions such as pH, temperature, addition of metal ions, surfactant and inhibitor agent. The specific activity measurements of all the crude enzymes result in the highest value of cellulase activity produced by isolate C12 which was 0.401 ± 0.018 U/mg. This enzyme activity was known to be optimum at 50°C and pH 9. It was also stimulated by K+, Na+, Mg2+, Fe3+, and SDS. However, the enzyme activity was inhibited by EDTA at 10 mM concentration. The use of coffee industry by-products as the source of cellulolytic microorganisms offers a promising approach for its various types of indigenous microorganisms and their unique property of cellulase produced that is useful for industrial application.

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ENZYME ACTIVITY IN TABASCO FRUIT SEPARATION ZONES

HortScience ◽

10.21273/hortsci.31.5.746a ◽

1996 ◽

Vol 31 (5) ◽

pp. 746a-746

Author(s):

Ramón A. Arancibia ◽

Carl E. Motsenbocker

Keyword(s):

Enzyme Activity ◽

Fruit Ripening ◽

Preliminary Data ◽

Carboxymethyl Cellulose ◽

Cellulase Activity ◽

Viscosity Reduction ◽

Capsicum Frutescens ◽

Detachment Force ◽

Cellulase Enzyme ◽

Separation Zones

Red-mature Tabasco (Capsicum frutescens) fruit (`McIlhenny Select') normally separate easily at the junction of the fruit and receptacle or calyx. Differences in fruit detachment force (FDF) between two lines, one that separates readily (`McIlhenny Select') and one that does not (`Hard Pick'), have been reported previously (Motsenbocker et al., 1995). In this study, enzyme activity from the detachment area was analyzed by viscosity reduction. The reaction mixture was 0.3% pectin in 20 mm NaAc, pH 5.5, for polygalacturonase (PG) and 0.6% carboxymethyl cellulose (CMC) in 20 mm NaPO4, pH 6.0, for cellulase. Preliminary data indicated that PG and cellulase enzyme activity increased during fruit ripening in both lines. Only cellulase activity, however, correlated with FDF. In addition, the activity of both enzymes was higher in the `McIlhenny Select' line than the `Hard Pick' line at the orange and red-mature stages.

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