scholarly journals Produksi Enzim Selulase olehAspergillus niger pada Ampas Sagu

2015 ◽  
Vol 16 (1) ◽  
pp. 1 ◽  
Author(s):  
Nora Idiawati ◽  
Elliska Murni Harfinda ◽  
Lucy Arianie
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Cellulase Enzymes ◽  
Fermentation Time ◽  
Cellulase Enzyme ◽  
Sago Waste ◽  
Solid Fermentation

Production of cellulase by Aspergillus niger was carried out by growing the cultureson sago waste. Sago waste containscellulose that has not been used optimally. Cellulose is a polysaccharide consisting of glucose monomers linked by β-1,4-glycosides bonds. Glycoside bonds in cellulose can be enzymatically hydrolyzed into glucose with cellulase enzymes. Solid fermentation used to produce cellulase on sago waste as substrate was influenced by pH (3 to 6), moisture content(40% to 85%), and fermentation time (4 to 10 days). Products of the cellulase enzyme activity was measured by phenolsulfuricacid method. The results showed that the highest cellulase enzyme activity was 0.172 U/mL obtained at 85%moisture content, pH 5, and 8 days of fermentation time.

scholarly journals Cellulase Enzyme Production From Rice Straw Using Solid State Fermentation and Fungi Aspergillus niger ITBCC L74

2018 ◽  
Vol 156 ◽  
pp. 01010 ◽  
Author(s):  
Siti Maftukhah ◽  
Abdullah Abdullah
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Protein Content ◽  
Rice Straw ◽  
Lignin Content ◽  
Enzyme Characterization ◽  
Raw Material ◽  
Enzymatic Reactions ◽  
Fermentation Time ◽  
Cellulase Enzyme

Rice straw is one of very abundant waste of agricultural and has not utilized maximally. This waste contain cellulose and potential in the manufacture of cellulase enzymes. Research on the production of cellulase enzyme from lignocellulose has been done a lot of enzyme activity is still low. This research using cellulose is 71.95% and conducted with 6 stages. First, the preparation of raw material. Second, the decrease of lignin content with alkali pretreatment. Third, the breeding of fungi Aspergillus niger ITBCC L74 . Fourth, incubation in the inoculum. Fifth, the production of cellulase enzyme by solid fermentation method. Finally, the analysis includes protein content, enzyme activity, enzyme characterization and kinetics of enzymatic reactions. The highest enzyme activity of this study is 3.12 U/ml and protein content is 0.34 mg/ml with fermentation time is 4 day and water content is 75%. In enzyme characterization obtained optimum pH and temperature are 4 and 60°C, respectively. And obtained paramatic kinetic are Vmax and Km for 40, 50, 60 and 70°C temperature are Vmax: 6.42; 4.7; 5.82 and 4.46 U/ml and Km : 1.32; 0.38; 0.32; and 0.12%, respectively.

Optimization of Cellulase Production Using Agricultural Wastes by Artificial Neural Network and Genetic Algorithm

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
Chun Chang ◽  
Guizhuan Xu ◽  
Junfang Yang ◽  
Duo Wang
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Fermentation Time

The cellulase production by Trichoderma viride was optimized using artificial intelligence-based techniques under solid state fermentation. In this study, a back propagation network was designed with Levenberg-Marquardt training algorithm, and the tangent sigmoid and pure linear functions were used as the transfer functions in the hidden and output layers of the ANN, respectively. An artificial neural network coupling genetic algorithms was used to optimize the process parameters, which include the mass ratio of wheat straw to wheat bran, moisture content and fermentation time. The ultimate process parameters of optimization were mass ration of wheat straw to wheat bran 2.9, moisture content 69.6 percent, and fermentation time 123.3h. Further test experiment showed that the final cellulase activity can reach to 11.62 U/g, which was the highest value among all the experimental results. This result indicates that the genetic algorithm based on a neural network model is a better optimization method for cellulase production in solid state fermentation. To improve the cellulase production, a mixed culture system of Trichoderma viride and Aspergillus niger was also developed. The cellulase activity increased by 7.40 percent with the addition of Aspergillus niger at 72h.

scholarly journals The Effect of Addition Mn2+ Metal Ions and Incubation Time to Bacillus cereus Cellulase Enzyme Activity from Endophytic Bacteria of Curcuma Rhizome (Curcuma zanthorrizha Roxb.)

el–Hayah ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 62-69
Author(s):  
Cahyaning Sulistyantini ◽  
Ulfah Utami
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Bacillus Cereus ◽  
Incubation Time ◽  
Endophytic Bacteria ◽  
Cellulase Enzymes ◽  
Cellulase Enzyme ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test

Cellulase is one of the most widely used enzymes in the industrial world and wastes decomposition process. Bacillus cereus is one of the bacteria that can produce cellulase enzymes that can hydrolyze cellulose to glucose. The addition of cofactors and incubation time can help determine the optimum conditions needed by cellulase enzymes to work optimally. This study aims to determine the effect of adding Mn2+ metal ions and incubation time to the activity of cellulase enzymes from Bacillus cereus endophytic bacteria. This research is experimentally used a Completely Randomized Design (CRD) factorial design with two factor treatments and 3 times repetitions. The first factor is variation of addition Mn2+ metal ions which are 5 mM, 10 mM, and 15 mM, the second is variation of incubation time which are 1 hour, 2 hours, 3 hours, 4 hours, and 5 hours. The data were analyzed using Analysis Of Variance (ANOVA) and if the data significantly affected the parameter, then it would be continued by Duncan Multiple Range Test (DMRT) with the fault level of 5%. The result showed that the interaction of addition Mn2+ metal ions and incubation time affected the cellulase enzyme activity of Bacillus cereus from endophytic bacteria. The highest cellulase enzyme activity obtained from interaction addition Mn2+ metal 10 mM and incubation time 3 hours with an activity 0,335 U/mL, while the lowest cellulase enzyme activity obtained from interaction addition Mn2+ metal 15 mM and incubation time 5 hours with an activity of 0,073 U/mL

scholarly journals Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province

DEPIK ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 243-250
Author(s):  
Irma Dewiyanti ◽  
Darmawi Darmawi ◽  
Zainal Abidin Muchlisin ◽  
Teuku Zahrial Helmi ◽  
Iko Imelda Arisa ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Methyl Cellulose ◽  
Cellulase Activity ◽  
Mangrove Ecosystem ◽  
Microbiology Laboratory ◽  
Cellulolytic Bacteria ◽  
Cellulase Enzymes ◽  
Cellulase Enzyme ◽  
Aceh Province

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian //  TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

scholarly journals The Effect Of Cellulase Content On Phytoestrogens Formation (Genistein) Materials On Fermented Soybean Waste Aspergillus niger

2018 ◽  
Vol 3 (1) ◽  
pp. 41-47
Author(s):  
Vara Tassa Sutari ◽  
Nurliana Nurliana ◽  
Sugito Sugito ◽  
Darmawi Darmawi ◽  
Rinidar Rinidar
Keyword(s):  
Enzyme Activity ◽  
Data Analysis ◽  
Statistical Analysis ◽  
Aspergillus Niger ◽  
Incubation Time ◽  
Fermentation Process ◽  
T Test ◽  
Fermented Soybean ◽  
Cellulase Enzyme ◽  
The Relationship

The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P <0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.

scholarly journals PRODUKSI ENZIM KITINASE DARI Aspergillus niger MENGGUNAKAN LIMBAH CANGKANG RAJUNGAN SEBAGAI INDUSER

2016 ◽  
Vol 1 (1) ◽  
pp. 34 ◽  
Author(s):  
Purkan Purkan ◽  
Afaf Baktir ◽  
Arju Rohmah Sayyidah
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Cell Mass ◽  
Hydrolytic Enzyme ◽  
Ph Optimum ◽  
Fermentation Time ◽  
Activity Test ◽  
Crab Shell Waste ◽  
Dry Cell ◽  
Local Nature

AbstrakKitinase merupakan enzim hidrolitik yang dapat menghidrolisis kitin pada ikatan β-1,4-glikosidiknya dengan menghasilkan derivat-derivat kitin seperti oligomer kitin yang mempunyai banyak manfaat. Penelitian ini bertujuan untuk melakukan pengembangan produksi enzim kitinase dari sumber lokal yang melimpah di alamserta murah dengan melakukan optimasi substrat dalam hal ini digunakan substrat tetes tebu (molase) dan limbah cangkang rajungan untuk produksi enzim kitinase dari Aspergillus niger. Sebelumnya, dilakukan kultivasi isolat kapang Aspergillus niger dengan membuat kurva pertumbuhan menggunakan metode masa sel kering dimana dari hasil penelitian inokulasi optimal adalah 22 jam. Pada proses produksi, diperoleh waktu fermentasi optimal adalah 52 jam dengan menentukan uji aktivitasnya menggunakan metode turbidimetri. Hasil optimasi substrat menunjukkan bahwa enzim kitinase yang maksimal diperoleh pada penambahan molase 0,5% (b/v) dengan unit aktivitas enzim 0,14726 (U/mL) dan cangkang rajungan 2% (b/v) dengan unit aktivitas enzim yang dihasilkan 0,12826 (U/mL). Kitinase dari Aspergillus niger ini mempunyai pH optimal 6 dan suhu optimal 40 oC. Kata kunci: Aspergillus niger, kitinase, cangkang rajungan, molase   AbstractChitinase is a hydrolytic enzyme that hydrolyzes chitin on β-1,4-glycosidic bond and thereby producing chitin derivatives such as chitin oligomers that have multiple benefits. The purpose of this research was to develop the production of chitinase enzyme from cheap and are abundant local nature sources, by optimizations substrate in this case the substrate used molasses and crab shell waste for the production of chitinase enzyme from Aspergillus niger. Previously, isolates of Aspergillus niger cultivated by creating a growth curve using dry cell mass method which from the results of research inoculation optimal are 22 hours. In the production process, obtained the optimum fermentation time is 52 hours to determine the activity test using turbidimetry method. Result of substrate optimizations indicate that chitinase enzyme maximum by addition of molasses obtained in 0.5% (w/v) with enzyme activity units 0.14726 (U/mL) and crab shells 2% (w/v) with enzyme activity units 0.12826 (U/mL). Chitinase from Aspergillus niger has a pH optimum 6 and temperature optimum 40 oC. Keywords: Aspergillus niger, chitinase, crab shells, molasses

scholarly journals Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium

2017 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Sri Rulianah ◽  
Zakijah Irfin ◽  
Mufid Mufid ◽  
Prayitno Prayitno
Keyword(s):  
Enzyme Activity ◽  
Salicylic Acid ◽  
Phanerochaete Chrysosporium ◽  
Substrate Concentration ◽  
Lignin Content ◽  
Raw Material ◽  
Cellulase Enzymes ◽  
Fermentation Time ◽  
Acid Reagent ◽  
High Cellulose

Bagasse mengandung selulosa yang cukup tinggi sehingga berpotensi sebagai bahan baku produksi crude selulase menggunakan kapang Phanerochaete chrysosporium. Kapang ini memiliki kemampuan untuk memproduksi enzim selulase dari substrat yang mengandung selulosa dan juga menghasilkan enzim yang dapat memecah lignin sehingga tidak perlu dilakukan proses delignifikasi. Tujuan dari penelitian ini adalah untuk memanfaatkan limbah ampas tebu sebagai bahan baku pembuatan crude selulase menggunakan kapang Phanerochaete chrysosporium dan mengetahui pengaruh penambahan konsentrasi substrat dan waktu fermentasi terhadap aktivitas crude selulase yang dihasilkan. Penelitian ini dilakukan dengan cara mengeringkan dan memperkecil ukuran ampas tebu, meremajakan kapang Phanerocheate chrysoporium, membuat inokulum dalam media cair, memfermentasi ampas tebu sesuai dengan variabel, dengan media Nitrogen Limited Media (NLM) menggunakan kapang Phanerocheate chrysoporium. Hasil fermentasi disaring, dan filtratnya dianalisa aktivitasnya sebagai crude selulase. Variabel dalam penelitian ini adalah waktu fermentasi 9, 11, 13, 15 dan 17 hari dan konsentrasi ampas tebu sebagai media: 5, 6, dan 7 % b/v. Ekstrak kasar selulase (crude) yang dihasilkan disaring menggunakan filter vakum, dan aktivitas filtrat (crude cellulase) diuji dengan pereaksi DNS (dinitro salicylic acid) dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa aktivitas selulase tertinggi diperoleh pada variabel konsentrasi ampas tebu sebesar 7% b/v dan waktu inkubasi selama 17 hari yaitu sebesar 91.304 U/mL.Bagasse contain high cellulose which potentially to be used to raw material for producing cellulase enzyme using fungi Phanerochaete chrysosporium. This fungus has ability to produce cellulase enzymes from substrates which contain cellulose and also produce enzymes that can degrade lignin content so it didn’t need the delignification process. The objective of this study was to convert cellulose in bagasse to be crude cellulase enzymes by using Phanerochaete chrysosporium and determine the effect of substrate concentration and fermentation time to the enzyme activity. This research was conducted by drying and reducing the bagasse particle size, rejuvenating mold Phanerocheate chrysoporium, making inoculum in liquid medium, fermenting bagasse in accordance with the variable, with media NLM (nitrogen limited media) using Phanerocheate chrysoporium. Fermentation results were filtered, and it was analyzed the activity of crude cellulase. The variable in this study was the time of fermentation 9, 11, 13, 15, and 17 days and substrate concentration: 5, 6, and 7 % b/v. Crude cellulose was filtered and was analyzed the enzyme activity by DNS (dinitro salicylic acid) reagent, using UV-Vis spectrophotometer. The best result of this study was the crude cellulase with highest activity 91,304 U/mL for 7 % substrate concentration with fermentation time 17 days.

scholarly journals Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

2011 ◽  
Vol 29 (No. 5) ◽  
pp. 557-567 ◽  
Author(s):  
X.-J. Dai ◽  
M.-Q. Liu ◽  
H.-X. Jin ◽  
M.-Y. Jing
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Xylanase Activity ◽  
Orange Peel ◽  
Basic Medium ◽  
Relative Molecular Mass ◽  
Xylanase Production ◽  
Fermentation Time

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P &lt; 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7&nbsp;U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5&nbsp;U/g). Optimum temperature and pH for XylA activity were 55&deg;C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited K<sub>m</sub> and V<sub>max </sub>values of 9.24 mg/ml and 54.05&nbsp;&mu;mol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

scholarly journals Pengaruh Penambahan NaOH Terhadap Produksi Gula Pereduksi dari Tongkol Jagung (Zea mays L.) oleh Aspergillus niger

2019 ◽  
Vol 19 (2) ◽  
pp. 212
Author(s):  
Norman Adi Husain ◽  
Taty Sulastry ◽  
Sudding Sudding
Keyword(s):  
Zea Mays ◽  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Zea Mays L ◽  
Enzyme Activity Assay ◽  
Cellulase Enzyme ◽  
Corn Cobs ◽  
Key Word ◽  
Solution Preparation ◽  
Hydrolysis Of

ABSTRAK Penelitian ini menggunakan tongkol jagung (Zea mays L.) yang jarang dimanfaatkan dan menjadi limbah untuk menghasilkan glukosa dengan mengoptimalkan kadar NaOH sebagai bahan untuk delignifikasi. Tahap-tahapnya meliputi preparasi sampel, proses delignifikasi, perbanyakan kapang Aspergillus niger, pembuatan larutan nutrisi, produksi enzim, ekstraksi enzim, uji aktivitas enzim selulase, hidrolisis sampel, dan analisis kadar glukosa. Kadar optimum NaOH yang digunakan untuk mendelignifikasi sampel tongkol jagung adalah 10% dengan rendemen sebesar 29,8800 %. Aktivitas enzim selulase sebesar 0,00372 U/mL. Kadar Glukosa yang dihasilkan pada proses hidrolisis tongkol jagung hasil delignifikasi NaOH 10, 15, 20, 25, dan 30 % masing-masing sebesar 14,269 x 103 mg.Kg-1; 9,404 x 103 mg.Kg-1; 11,982 x 103 mg.Kg-1; 11,310 x 103 mg.Kg-1; dan 12,254 x 103 mg.Kg-1. Kata kunci : Selulosa, tongkol jagung, delignifikasi, kadar glukosa ABSTRACT This study uses corn cobs (Zea mays L.) that barely use and become waste to produce glucose by optimizing the concentration of NaOH as a material for delignification. The stages include of sample preparation, delignification process, multiplication of Aspergillus niger, nutrient solution preparation, production of enzymes, extraction of the enzyme, cellulase enzyme activity assay, hydrolysis of samples, and analyzes concentration of glucose. Optimum levels of NaOH for delignification is 10% with a yield of 29.8800 %. Cellulase enzyme activity is about 0.00372 U/mL. Concentration of Glucose produced in the process of hydrolysis of corn cob delignificated with NaOH 10, 15, 20, 25, and 30% respectively of 14.269 x 103 mg.Kg-1; 9.404 x 103 mg.Kg-1; 11.982 x 103 mg.Kg-1; 11.310 x 103 mg.Kg-1; and 12.254 x 103 mg.Kg-1. Key word : Cellulose, corn cobs, delignification, glucose level

scholarly journals The Activity Of Cellulose Enzyme From Indigenous Bacteria "Bacillus Sp YLB1" As Bioactivator

2019 ◽  
Vol 7 (2) ◽  
pp. 10-18
Author(s):  
Yunilas ◽  
Lili Warly ◽  
Yetti Marli ◽  
Irsan Riyanto
Keyword(s):  
Enzyme Activity ◽  
Palm Oil ◽  
Palm Kernel ◽  
Bacillus Sp ◽  
Palm Kernel Cake ◽  
Optimal Result ◽  
Fermentation Time ◽  
Indigenous Bacteria ◽  
Cellulase Enzyme

This study aims to test the activity of cellulase enzyme (CMCase) fromindigenous bacteria "Bacillus Sp YLB1" as bioactivator of palm oil based feed. Thetreatment consists of various combinations of substart, namely: P1 = 80% palm leaves +10% palm kernel cake + 10% sludge; P2 = 60% palm leaves + 20% palm kernel cake +20% sludge and P3 = 60% palm leaves + 30% palm kernel cake+ 30% sludge. The parameters observed were cellulase enzyme activity (CMCase) onvarious combinations of palm oil waste and fermentation time. The results showed thatcellulase enzyme (CMCase) production of Bacillus sp YLB1 bacteria during fermentationfluctuated ie 0.143 Units / ml; 0.372 Units / ml; 0.588 Units / ml; 1.013 Units / ml; 0.906Units / ml; 1,065 Units / ml and 1,198 Units / ml.The activity of cellulase enzyme(CMCase) on substrate P1 is lower than P2 and P3. As conclutions that cellulase enzymeactivity of Bacillus sp YLB1 influenced by the combination of substrate and fermentationtime. The P3 fermented with Bacillus sp YLB1 for 7 days gave a more optimal result thanthe other treatments

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