Immunohistochemical Characterization of Tumor Cells and Inflammatory Infiltrate Associated with Cutaneous Melanocytic Tumors of Duroc and Iberian Swine

2002 ◽  
Vol 39 (4) ◽  
pp. 445-451 ◽  
Author(s):  
J. Pérez ◽  
P. M. García ◽  
M. J. Bautista ◽  
Y. Millán ◽  
J. Ordás ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cells ◽  
Inflammatory Infiltrate ◽  
Plasma Cells ◽  
Class Ii ◽  
Neoplastic Cells ◽  
Melanocytic Tumors ◽  
Alpha 1 Antitrypsin ◽  
Cellular Immune ◽  
Residual Lesions

The immunophenotype of tumor cells and inflammatory infiltrate associated with cutaneous melanocytic lesions (29 melanocytomas, two malignant melanomas, and 23 residual lesions) from 54 adult Iberian and Iberian X Duroc pigs were examined using a panel of nine antibodies. All neoplastic cells were vimentin−, cytokeratin−, and alpha-1-antitrypsin− and the majority were S100+, whereas all pigmented macrophages were vimentin+, cytokeratin−, and S100− and most expressed alpha-1-antitrypsin. Regressing tumors were characterized by zones with low density of neoplastic cells accompanied by heavy infiltration of CD3+ T lymphocytes, whereas zones with high density of neoplastic cells showed very low numbers of CD3+ T lymphocytes. The infiltrate of CD79a+ B cells and IgG, IgM, and IgA plasma cells was low. The majority of lymphocytes of the peri- and intratumoral infiltrate were major histocompatibility complex class II+, but neoplastic cells did not express class II antigen. The 17 residual lesions examined were composed of macrophages containing abundant melanin pigment and low to moderate numbers of CD3+ T lymphocytes. The results of the present study suggest that the local cellular immune response plays a crucial role in the host response that induces regression of cutaneous melanomas and melanocytomas of the Iberian and crossbred Iberian X Duroc pigs.

Immunophenotype of the inflammatory response in the central and enteric nervous systems of cockatiels (Nymphicus hollandicus) experimentally infected with parrot bornavirus 2

2022 ◽  
pp. 030098582110691
Author(s):  
Jeann Leal de Araújo ◽  
Raquel R. Rech ◽  
Aline Rodrigues-Hoffmann ◽  
Paula R. Giaretta ◽  
Cinthya Cirqueira ◽  
...  
Keyword(s):  
Small Intestine ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Inflammatory Infiltrate ◽  
Homogeneous Distribution ◽  
Nymphicus Hollandicus ◽  
Inflammatory Infiltrates ◽  
Cellular Immune ◽  
Proventricular Dilatation Disease ◽  
The Brain

Proventricular dilatation disease is a lethal disease of psittacine birds. In this study, we characterized the local cellular immune response in the brain, proventriculus, and small intestine of 27 cockatiels ( Nymphicus hollandicus) experimentally infected with parrot bornavirus 2 (PaBV-2). Perivascular cuffs in the brain were composed of CD3+ T-lymphocytes and Iba1+ macrophages/microglia in most cockatiels (n = 26). In the ganglia of the proventriculus, CD3+ T-lymphocytes (n = 17) and Iba1+ macrophages (n = 13) prevailed. The ganglia of the small intestine had a more homogeneous distribution of these leukocytes, including PAX5+ B-lymphocytes (n = 9), CD3+ T-lymphocytes (n = 8), and Iba1+ macrophages (n = 8). Our results indicate that perivascular cuffs in the brain and the inflammatory infiltrate in the proventriculus of PaBV-2-infected cockatiels is predominately composed of T-lymphocytes, while the inflammatory infiltrates in the ganglia of the small intestine are characterized by a mixed infiltrate composed of T-lymphocytes, B-lymphocytes, and macrophages.

Locally superantigen-activated peritoneal cytolytic T lymphocytes belong to the CD8+CD45RC− subset and lyse MHC class II+ tumor cells

1992 ◽  
Vol 34 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Johan Hansson ◽  
Per-Olof Ericsson ◽  
Mikael Dohlsten ◽  
Hans-Olov Sjögren ◽  
Terje Kalland ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cells ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Cytolytic T Lymphocytes

scholarly journals MHC Class II–Transduced Tumor Cells Originating in the Immune-Privileged Eye Prime and Boost CD4+ T Lymphocytes that Cross-react with Primary and Metastatic Uveal Melanoma Cells

2007 ◽  
Vol 67 (9) ◽  
pp. 4499-4506 ◽  
Author(s):  
Jacobus J. Bosch ◽  
James A. Thompson ◽  
Minu K. Srivastava ◽  
Uzoma K. Iheagwara ◽  
Timothy G. Murray ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cells ◽  
Mhc Class Ii ◽  
Uveal Melanoma ◽  
Class Ii ◽  
Melanoma Cells

scholarly journals Immunological and pathological investigations of non-specific reactive hepatitis in shelter dogs

2019 ◽  
Vol 69 (1) ◽  
pp. 61-72
Author(s):  
Jose Raduan Jaber ◽  
Alicia S Velázquez-Wallraf ◽  
David Farray ◽  
Antonio G. Ravelo-García ◽  
Conrado Carrascosa ◽  
...  
Keyword(s):  
Inflammatory Infiltrate ◽  
Plasma Cells ◽  
Inflammatory Diseases ◽  
Liver Parenchyma ◽  
Tissue Sections ◽  
Hla Dr ◽  
Organ Systems ◽  
Shelter Dogs ◽  
Alpha 1 Antitrypsin ◽  
Hematoxylin Eosin

Abstract The aim of this study was to describe the gross, histopathological and immunohistochemical features of non-specific reactive hepatitis (NSRH) in stray dogs. To perform this study the livers of 23 dogs of different breed and sex were used. The tissue sections were stained with hematoxylin–eosin, and immunohistochemically. The results of this work showed that NSRH was characterized by the infiltration of lymphocytes and plasma cells scattered throughout the liver parenchyma and in the portal stroma, and associated with inflammatory and non-inflammatory diseases in other organ systems. The inflammatory infiltrate of NSRH was composed of CD3+ T lymphocytes and HLA-DR+ lymphocytes, as well as IgG+ plasma cells and alpha-1-antitrypsin+ macrophages in the portal spaces and hepatic sinusoids.

scholarly journals The Caspase-1/IL-18 Axis of the Inflammasome in Tumor Cells: A Modulator of the Th1/Tc1 Response of Tumor-Infiltrating T Lymphocytes in Colorectal Cancer

Cancers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 189
Author(s):  
Linda Bilonda Mutala ◽  
Cécile Deleine ◽  
Matilde Karakachoff ◽  
Delphine Dansette ◽  
Kathleen Ducoin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
T Lymphocytes ◽  
Tumor Cells ◽  
Ex Vivo ◽  
Explant Culture ◽  
T Cell Response ◽  
Mrna Levels ◽  
Innate And Adaptive Immunity ◽  
Caspase 1 ◽  
Culture Model

In colorectal cancer (CRC), a high density of T lymphocytes represents a strong prognostic marker in subtypes of CRC. Optimized immunotherapy strategies to boost this T-cell response are still needed. A good candidate is the inflammasome pathway, an emerging player in cancer immunology that bridges innate and adaptive immunity. Its effector protein caspase-1 matures IL-18 that can promote a T-helper/cytotoxic (Th1/Tc1) response. It is still unknown whether tumor cells from CRC possess a functional caspase-1/IL-18 axis that could modulate the Th1/Tc1 response. We used two independent cohorts of CRC patients to assess IL-18 and caspase-1 expression by tumor cells in relation to the density of TILs and the microsatellite status of CRC. Functional and multiparametric approaches at the protein and mRNA levels were performed on an ex vivo CRC explant culture model. We show that, in the majority of CRCs, tumor cells display an activated and functional caspase-1/IL-18 axis that contributes to drive a Th1/Tc1 response elicited by TILs expressing IL-18Rα. Furthermore, unsupervised clustering identified three clusters of CRCs according to the caspase-1/IL-18/TIL density/interferon gamma (IFNγ) axis and microsatellite status. Together, our results strongly suggest that targeting the caspase-1/IL-18 axis can improve the anti-tumor immune response in subgroups of CRC.

scholarly journals Macrophages and microglia: the cerberus of glioblastoma

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Alice Buonfiglioli ◽  
Dolores Hambardzumyan
Keyword(s):  
Tumor Cells ◽  
Innate Immune System ◽  
Tumor Heterogeneity ◽  
Expression Profiles ◽  
Brain Parenchyma ◽  
Innate Immune ◽  
Malignant Growth ◽  
Neoplastic Cells ◽  
Functional Roles ◽  
The Brain

AbstractGlioblastoma (GBM) is the most aggressive and deadliest of the primary brain tumors, characterized by malignant growth, invasion into the brain parenchyma, and resistance to therapy. GBM is a heterogeneous disease characterized by high degrees of both inter- and intra-tumor heterogeneity. Another layer of complexity arises from the unique brain microenvironment in which GBM develops and grows. The GBM microenvironment consists of neoplastic and non-neoplastic cells. The most abundant non-neoplastic cells are those of the innate immune system, called tumor-associated macrophages (TAMs). TAMs constitute up to 40% of the tumor mass and consist of both brain-resident microglia and bone marrow-derived myeloid cells from the periphery. Although genetically stable, TAMs can change their expression profiles based upon the signals that they receive from tumor cells; therefore, heterogeneity in GBM creates heterogeneity in TAMs. By interacting with tumor cells and with the other non-neoplastic cells in the tumor microenvironment, TAMs promote tumor progression. Here, we review the origin, heterogeneity, and functional roles of TAMs. In addition, we discuss the prospects of therapeutically targeting TAMs alone or in combination with standard or newly-emerging GBM targeting therapies.

Unusual childhood extramedullary hematologic malignancy with natural killer cell properties that contains tropomyosin 4–anaplastic lymphoma kinase gene fusion

Blood ◽  
2001 ◽  
Vol 98 (4) ◽  
pp. 1209-1216 ◽  
Author(s):  
Sandra J. Meech ◽  
Loris McGavran ◽  
Lorrie F. Odom ◽  
Xiayuan Liang ◽  
Lynne Meltesen ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Tumor Cells ◽  
Functional Properties ◽  
Anaplastic Lymphoma Kinase ◽  
Clinical Presentation ◽  
Hematologic Malignancy ◽  
Malignant Cells ◽  
Neoplastic Cells ◽  
Anaplastic Lymphoma

This report describes an unusual extramedullary hematologic malignancy in an 18-month-old child who presented with a capillary leak syndrome that evolved into hyperleukocytosis with malignant cells. The circulating tumor cells did not express an antigen profile typical of any subtype of leukemia commonly observed in children. Tumor cells were CD3−/CD56+; had germline TCRgenes; and strongly expressed CD30, epithelial membrane antigen, and anaplastic lymphoma kinase (ALK) consistent with a null cell anaplastic large cell lymphoma (ALCL). The malignant cells contained a t(2;19)(p23;p13.1) that interrupted ALK and translocated it to the der(19). Reverse transcriptase-polymerase chain reaction and nucleotide sequence analysis revealed fusion of ALK to tropomyosin 4, an ALK fusion partner not described previously in hematologic malignancies. The clinical presentation and phenotypic features of this malignancy were not typical for ALCL because tumor cells expressed both myeloid (CD13, CD33, HLA-DR) and natural killer (NK) cell antigens. The neoplastic cells most resembled NK cells because in addition to being CD3−/CD56+ with germline TCR genes, these cells were CD25+/CD122+/granzyme B+ and possessed the functional properties of immature NK cells. The unusual clinical presentation, immunophenotype, and functional properties of these neoplastic cells suggest that this malignancy may be derived from the putative myeloid-NK precursor cell. Furthermore co-expression of NK and ALCL features supports the concept that a minority of null-ALCL may be derived from NK cells and expands the spectrum of phenotypes that can be seen in tumors produced by ALK fusion proteins.

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