BRCA1 Haploinsufficiency Leads to Altered Expression of Genes Involved in Cellular Proliferation and Development

A comparative study was carried out on common and agile frogs ( Rana temporaria and R. dalmatina) naturally infected with ranid herpesvirus 3 (RaHV3) and common toads ( Bufo bufo) naturally infected with bufonid herpesvirus 1 (BfHV1) to investigate common pathogenetic pathways and molecular mechanisms based on macroscopic, microscopic, and ultrastructural pathology as well as evaluation of gene expression. Careful examination of the tissue changes, supported by in situ hybridization, at different stages of development in 6 frogs and 14 toads revealed that the skin lesions are likely transient, and part of a tissue cycle necessary for viral replication in the infected hosts. Transcriptomic analysis, carried out on 2 naturally infected and 2 naïve common frogs ( Rana temporaria) and 2 naturally infected and 2 naïve common toads ( Bufo bufo), revealed altered expression of genes involved in signaling and cell remodeling in diseased animals. Finally, virus transcriptomics revealed that both RaHV3 and BfHV1 had relatively high expression of a putative immunomodulating gene predicted to encode a decoy receptor for tumor necrosis factor in the skin of the infected hosts. Thus, the comparable lesions in infected frogs and toads appear to reflect a concerted epidermal and viral cycle, with presumptive involvement of signaling and gene remodeling host and immunomodulatory viral genes.

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Matching the Heart to Heat-Induced Circulatory Load: Heat-Acclimatory Responses

Physiology ◽

10.1152/nips.01453.2003 ◽

2003 ◽

Vol 18 (6) ◽

pp. 215-221 ◽

Cited By ~ 5

Author(s):

Michal Horowitz

Keyword(s):

Heart Rate ◽

Oxygen Demand ◽

Heat Acclimation ◽

Regulatory Proteins ◽

Contractile Apparatus ◽

Cardiac Efficiency ◽

Cross Tolerance ◽

Altered Expression ◽

Expression Of Genes ◽

Cardiac Adaptation

Heat acclimation enhances cardiac efficiency by increasing stroke volume and decreasing heart rate. These adaptations involve biochemical changes in the contractile apparatus, switched on by altered expression of genes coding contractile and calcium-regulatory proteins and partially mediated by persistent low thyroxine. Heat acclimation also produces cross-tolerance to oxygen deprivation, thus reinforcing cardiac adaptation to oxygen demand/supply mismatching via energy-sparing pathways.

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Altered expression of genes regulating angiogenesis in experimental androgen-independent prostate cancer

The Prostate ◽

10.1002/pros.20672 ◽

2007 ◽

Vol 68 (2) ◽

pp. 161-170 ◽

Cited By ~ 31

Author(s):

Heléne Gustavsson ◽

Karin Jennbacken ◽

Karin Welén ◽

Jan-Erik Damber

Keyword(s):

Prostate Cancer ◽

Altered Expression ◽

Expression Of Genes ◽

Androgen Independent

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Depletion of KNL2 Results in Altered Expression of Genes Involved in Regulation of the Cell Cycle, Transcription, and Development in Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms20225726 ◽

2019 ◽

Vol 20 (22) ◽

pp. 5726 ◽

Cited By ~ 1

Author(s):

Anastassia Boudichevskaia ◽

Andreas Houben ◽

Anne Fiebig ◽

Klara Prochazkova ◽

Ales Pecinka ◽

...

Keyword(s):

Cell Cycle ◽

Critical Role ◽

Histone H3 ◽

Global Gene Expression ◽

Proper Function ◽

Knockout Mutant ◽

Flower Bud ◽

Altered Expression ◽

Expression Of Genes ◽

Histone H3 Variant

Centromeres contain specialized nucleosomes at which histone H3 is partially replaced by the centromeric histone H3 variant cenH3 that is required for the assembly, maintenance, and proper function of kinetochores during mitotic and meiotic divisions. Previously, we identified a KINETOCHORE NULL 2 (KNL2) of Arabidopsis thaliana that is involved in the licensing of centromeres for the cenH3 recruitment. We also demonstrated that a knockout mutant for KNL2 shows mitotic and meiotic defects, slower development, reduced growth rate, and fertility. To analyze an effect of KNL2 mutation on global gene transcription of Arabidopsis, we performed RNA-sequencing experiments using seedling and flower bud tissues of knl2 and wild-type plants. The transcriptome data analysis revealed a high number of differentially expressed genes (DEGs) in knl2 plants. The set was enriched in genes involved in the regulation of the cell cycle, transcription, development, and DNA damage repair. In addition to comprehensive information regarding the effects of KNL2 mutation on the global gene expression, physiological changes in plants are also presented, which provides an integrated understanding of the critical role played by KNL2 in plant growth and development.

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Age-dependent changes in electrophysiology and calcium handling – implications for pediatric cardiac research

10.1101/657551 ◽

2019 ◽

Author(s):

Luther M. Swift ◽

Morgan Burke ◽

Devon Guerrelli ◽

Manelle Ramadan ◽

Marissa Reilly ◽

...

Keyword(s):

Postnatal Development ◽

Cardiac Electrophysiology ◽

Calcium Handling ◽

Cell Coupling ◽

Altered Expression ◽

Expression Of Genes ◽

Age Dependent ◽

Genes Encoding ◽

Cardiac Maturation

ABSTRACTRationaleThe heart continues to develop and mature after birth and into adolescence. Accordingly, cardiac maturation is likely to include a progressive refinement in both organ morphology and function during the postnatal period. Yet, age-dependent changes in cardiac electrophysiology and calcium handling have not yet been fully characterized.ObjectiveThe objective of this study, was to examine the relationship between cardiac maturation, electrophysiology, and calcium handling throughout postnatal development in a rat model.MethodsPostnatal rat cardiac maturation was determined by measuring the expression of genes involved in cell-cell coupling, electrophysiology, and calcium handling. In vivo electrocardiograms were recorded from neonatal, juvenile, and adult animals. Simultaneous dual optical mapping of transmembrane voltage and calcium transients was performed on isolated, Langendorff-perfused rat hearts (postnatal day 0–3, 4-7, 8-14, adult).ResultsYounger, immature hearts displayed slowed electrical conduction, prolonged action potential duration and increased ventricular refractoriness. Slowed calcium handling in the immature heart increased the propensity for calcium transient alternans which corresponded to alterations in the expression of genes encoding calcium handling proteins. Developmental changes in cardiac electrophysiology were associated with the altered expression of genes encoding potassium channels and intercalated disc proteins.ConclusionUsing an intact whole heart model, this study highlights chronological changes in cardiac electrophysiology and calcium handling throughout postnatal development. Results of this study can serve as a comprehensive baseline for future studies focused on pediatric cardiac research, safety assessment and/or preclinical testing using rodent models.

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Maternal iron deficiency alters trophoblast differentiation and placental development in rat pregnancy

Endocrinology ◽

10.1210/endocr/bqab215 ◽

2021 ◽

Author(s):

Hannah Roberts ◽

Andrew G Woodman ◽

Kelly J Baines ◽

Mariyan J Jeyarajah ◽

Stephane L Bourque ◽

...

Keyword(s):

Iron Deficiency ◽

Fetal Growth ◽

Growth And Development ◽

Iron Homeostasis ◽

Junctional Zone ◽

Altered Expression ◽

Expression Of Genes ◽

Iron Deficient ◽

Increased Risk ◽

Maternal Iron

Abstract Iron deficiency occurs when iron demands chronically exceed intake, and is prevalent in pregnant women. Iron deficiency during pregnancy poses major risks for the baby, including fetal growth restriction and long-term health complications. The placenta serves as the interface between a pregnant mother and her baby, and ensures adequate nutrient provisions for the fetus. Thus, maternal iron deficiency may impact fetal growth and development by altering placental function. We used a rat model of diet-induced iron deficiency to investigate changes in placental growth and development. Pregnant Sprague-Dawley rats were fed either a low-iron or iron-replete diet starting two weeks before mating. Compared to controls, both maternal and fetal hemoglobin were reduced in dams fed low-iron diets. Iron deficiency decreased fetal liver and body weight, but not brain, heart or kidney weight. Placental weight was increased in iron deficiency, due primarily to expansion of the placental junctional zone. The stimulatory effect of iron deficiency on junctional zone development was recapitulated in vitro, as exposure of rat trophoblast stem cells to the iron chelator deferoxamine increased differentiation toward junctional zone trophoblast subtypes. Gene expression analysis revealed 464 transcripts changed at least 1.5-fold (P<0.05) in placentas from iron-deficient dams, including altered expression of genes associated with oxygen transport and lipoprotein metabolism. Expression of genes associated with iron homeostasis was unchanged despite differences in levels of their encoded proteins. Our findings reveal robust changes in placentation during maternal iron deficiency, which could contribute to the increased risk of fetal distress in these pregnancies.

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Altered expression of genes regulating inflammation and synaptogenesis during regrowth of afferent neurons to cochlear hair cells

PLoS ONE ◽

10.1371/journal.pone.0238578 ◽

2020 ◽

Vol 15 (10) ◽

pp. e0238578

Author(s):

Chen-Chi Wu ◽

Aurore Brugeaud ◽

Richard Seist ◽

Hsiao-Chun Lin ◽

Wei-Hsi Yeh ◽

...

Keyword(s):

Hair Cells ◽

Afferent Neurons ◽

Cochlear Hair Cells ◽

Altered Expression ◽

Expression Of Genes

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ALTERED EXPRESSION OF GENES INVOLVED IN JAK-STAT, ADIPOCYTOKINE AND TOLL-LIKE RECEPTOR SIGNALING PATHWAYS ARE DETECTED IN BLASTOCOEL FLUID CONDITIONED MEDIA FROM EUPLOID EMBRYOS WITH POSITIVE IMPLANTATION OUTCOMES

Fertility and Sterility ◽

10.1016/j.fertnstert.2020.08.1062 ◽

2020 ◽

Vol 114 (3) ◽

pp. e356-e357

Author(s):

Arnav Lal ◽

Laura Grace Wolfe ◽

Trevor Shull ◽

Addison Paige Brandt ◽

Carson M. Collins ◽

...

Keyword(s):

Signaling Pathways ◽

Conditioned Media ◽

Receptor Signaling ◽

Toll Like Receptor ◽

Altered Expression ◽

Expression Of Genes

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Altered expression of selected genes in kidney of rats with lithium-induced NDI

AJP Renal Physiology ◽

10.1152/ajprenal.00305.2004 ◽

2005 ◽

Vol 288 (6) ◽

pp. F1276-F1289 ◽

Cited By ~ 45

Author(s):

Aleksandra Rojek ◽

Jakob Nielsen ◽

Heddwen L. Brooks ◽

Hong Gong ◽

Young-Hee Kim ◽

...

Keyword(s):

Gene Expression ◽

Protein Expression ◽

Cellular Proliferation ◽

Collecting Duct ◽

Lithium Treatment ◽

Altered Expression ◽

Ezrin Expression ◽

Microarray Screening ◽

And Control ◽

Thin Limb

Lithium treatment is associated with development of nephrogenic diabetes insipidus, caused in part by downregulation of collecting duct aquaporin-2 (AQP2) and AQP3 expression. In the present study, we carried out cDNA microarray screening of gene expression in the inner medulla (IM) of lithium-treated and control rats, and selected genes were then investigated at the protein level by immunoblotting and/or immunohistochemistry. The following genes exhibited significantly altered transcription and mRNA expression levels, and these were compatible with the changes in protein expression. 11β-Hydroxysteroid dehydrogenase type 2 protein expression in the IM was markedly increased (198 ± 25% of controls, n = 6), and immunocytochemistry demonstrated an increased labeling of IM collecting duct (IMCD) principal cells. This indicated altered renal mineralocorticoid/glucocorticoid responses in lithium-treated rats. The inhibitor of cyclin-dependent kinases p27 (KIP) protein expression was significantly decreased or undetectable in the IMCD cells, pointing to increased cellular proliferation and remodeling. Heat shock protein 27 protein expression was decreased in the IM (64 ± 6% of controls, n = 6), likely to be associated with the decreased medullary osmolality in lithium-treated rats. Consistent with this, lens aldose reductase protein expression was markedly decreased in the IM (16 ± 2% of controls, n = 6), and immunocytochemistry revealed decreased expression in the thin limb cells in the middle and terminal parts of the IM. Ezrin protein expression was upregulated in the IM (158 ± 16% of controls, n = 6), where it was predominantly expressed in the apical and cytoplasmic domain of the IMCD cells. Increased ezrin expression indicated remodeling of the actin cytoskeleton and/or altered regulation of IMCD transporters. In conclusion, the present study demonstrates changes in gene expression not only in the collecting duct but also in the thin limb of the loop of Henle in the IM, and several of these genes are linked to altered sodium and water reabsorption, cell cycling, and changes in interstitial osmolality.

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Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly

Parasites & Vectors ◽

10.1186/s13071-020-04433-3 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Sophie Tandonnet ◽

Gisele Antoniazzi Cardoso ◽

Pedro Mariano-Martins ◽

Raquel Dietsche Monfardini ◽

Vanessa A. S. Cunha ◽

...

Keyword(s):

Insecticide Resistance ◽

Molecular Basis ◽

Pyrethroid Resistance ◽

Management Strategies ◽

Evolutionary Process ◽

Specific Expression ◽

Altered Expression ◽

Functional Studies ◽

Expression Of Genes ◽

Starting Point

Abstract Background The emergence of insecticide resistance is a fast-paced example of the evolutionary process of natural selection. In this study, we investigated the molecular basis of resistance in the myiasis-causing fly Cochliomyia hominivorax (Diptera: Calliphoridae) to dimethyl-organophosphate (OP) insecticides. Methods By sequencing the RNA from surviving larvae treated with dimethyl-OP (resistant condition) and non-treated larvae (control condition), we identified genes displaying condition-specific polymorphisms, as well as those differentially expressed. Results Both analyses revealed that resistant individuals have altered expression and allele-specific expression of genes involved in proteolysis (specifically serine-endopeptidase), olfactory perception and cuticle metabolism, among others. We also confirmed that resistant individuals carry almost invariably the Trp251Ser mutation in the esterase E3, known to confer OP and Pyrethroid resistance. Interestingly, genes involved in metabolic and detoxifying processes (notably cytochrome P450s) were found under-expressed in resistant individuals. An exception to this were esterases, which were found up-regulated. Conclusions These observations suggest that reduced penetration and aversion to dimethyl-OP contaminated food may be important complementary strategies of resistant individuals. The specific genes and processes found are an important starting point for future functional studies. Their role in insecticide resistance merits consideration to better the current pest management strategies.

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