scholarly journals HemoDownloader: Open source software utility to extract data from HemoCue HbA1c 501 devices in epidemiological studies of diabetes mellitus

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242087
Author(s):  
Martin Rune Hassan Hansen ◽  
Vivi Schlünssen ◽  
Annelli Sandbæk
Keyword(s):  
Diabetes Mellitus ◽  
Open Source ◽  
Point Of Care ◽  
Epidemiological Studies ◽  
Digital Format ◽  
File Formats ◽  
Glycated Hemoglobin A ◽  
Serious Disease ◽  
Software Utility

Diabetes mellitus is a serious disease with increasing global prevalence. Point-of-care analysis of glycated hemoglobin A (HbA1c) holds promise as a diagnostic test for diabetes mellitus in epidemiological studies in challenging environments with limited access to centralized biochemical labs. The HemoCue HbA1c 501 device can be used for point-of-care determination of HbA1c, but its usability in epidemiological studies is limited by its inability to export results in digital format. We have developed the open source HemoDownloader software to overcome this limitation of the device. HemoDownloader has an easy-to-use graphical user interface and can export data from HemoCue HbA1c 501 to standard spreadsheet file formats. The program has the potential to improve data collection and management in epidemiological studies of diabetes mellitus.

scholarly journals Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 189
Author(s):  
Susana Campuzano ◽  
Paloma Yáñez-Sedeño ◽  
José Manuel Pingarrón
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Accurate Determination ◽  
Test Time ◽  
Electrochemical Biosensing ◽  
Recent Developments ◽  
Inflammatory Processes ◽  
Human Skills ◽  
Cancer Diseases

The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

A complete lab-made point of care device for non-immunological electrochemical determination of cortisol levels in salivary samples

2021 ◽  
Vol 332 ◽  
pp. 129532
Author(s):  
A. Gevaerd ◽  
E.Y. Watanabe ◽  
C. Belli ◽  
L.H. Marcolino-Junior ◽  
M.F. Bergamini
Keyword(s):  
Point Of Care ◽  
Electrochemical Determination ◽  
Cortisol Levels

scholarly journals Determination of amino acids in human biological fluids by high-performance liquid chromatography: critical review

Amino Acids ◽  
2021 ◽  
Author(s):  
Grażyna Gałęzowska ◽  
Joanna Ratajczyk ◽  
Lidia Wolska
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Analytical Techniques ◽  
Epidemiological Studies ◽  
Preparation Conditions

AbstractThe quantitation and qualification of amino acids are most commonly used in clinical and epidemiological studies, and provide an excellent way of monitoring compounds in human fluids which have not been monitored previously, to prevent some diseases. Because of this, it is not surprising that scientific interest in evaluating these compounds has resurfaced in recent years and has precipitated the development of a multitude of new analytical techniques. This review considers recent developments in HPLC analytics on the basis of publications from the last few years. It helps to update and systematize knowledge in this area. Particular attention is paid to the progress of analytical methods, pointing out the advantages and drawbacks of the various techniques used for the preparation, separation and determination of amino acids. Depending on the type of sample, the preparation conditions for HPLC analysis change. For this reason, the review has focused on three types of samples, namely urine, blood and cerebrospinal fluid. Despite time-consuming sample preparation before HPLC analysis, an additional derivatization technique should be used, depending on the detection technique used. There are proposals for columns that are specially modified for amino acid separation without derivatization, but the limit of detection of the substance is less beneficial. In view of the fact that amino acid analyses have been performed for years and new solutions may generate increased costs, it may turn out that older proposals are much more advantageous.

scholarly journals Screening Strategy of Pancreatic Cancer in Patients with Diabetes Mellitus

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 572
Author(s):  
Suguru Mizuno ◽  
Yousuke Nakai ◽  
Kazunaga Ishigaki ◽  
Kei Saito ◽  
Hiroki Oyama ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Pancreatic Cancer ◽  
Epidemiological Studies ◽  
Screening Strategy ◽  
Diabetic Patients ◽  
Reverse Causality ◽  
Pilot Studies ◽  
Patients With Diabetes ◽  
High Risk Populations ◽  
New Onset

The incidence of pancreatic cancer (PCa) is increasing worldwide and has become one of the leading causes of cancer-related death. Screening for high risk populations is fundamental to overcome this intractable malignancy. Diabetes mellitus (DM) is classically known as a risk factor for PCa. Recently the reverse causality is in the spotlight, that is to say, DM is considered to be a manifestation of PCa. Numbers of epidemiological studies clarified that new-onset DM (≤2-year duration) was predominant in PCa patients and the relative risk for PCa inversely correlated with duration of DM. Among patients with new-onset DM, elder onset, weight loss, and rapid exacerbation of glycemic control were reported to be promising risk factors and signs, and the model was developed by combining these factors. Several pilot studies disclosed the possible utility of biomarkers to discriminate PCa-associated DM from type 2 DM. However, there is no reliable biomarkers to be used in the practice. We previously reported the application of a multivariate index for PCa based on the profile of plasma free amino acids (PFAAs) among diabetic patients. We are further investigating on the PFAA profile of PCa-associated DM, and it can be useful for developing the novel biomarker in the near future.

scholarly journals Diabetes Mellitus, Cognitive Impairment, and Traditional Chinese Medicine

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
S. W. Seto ◽  
G. Y. Yang ◽  
H. Kiat ◽  
A. Bensoussan ◽  
Y. W. Kwan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Chinese Medicine ◽  
Side Effects ◽  
Traditional Chinese Medicine ◽  
Cognitive Dysfunction ◽  
Scientific Evidence ◽  
Cerebrovascular Disorders ◽  
Blood Glucose Control ◽  
Epidemiological Studies ◽  
Therapeutic Benefits

Diabetes mellitus (DM) is a metabolic disorder affecting a large number of people worldwide. Numerous studies have demonstrated that DM can cause damage to multiple systems, leading to complications such as heart disease, cancer, and cerebrovascular disorders. Numerous epidemiological studies have shown that DM is closely associated with dementia and cognition dysfunction, with recent research focusing on the role of DM-mediated cerebrovascular damage in dementia. Despite the therapeutic benefits of antidiabetic agents for the treatment of DM-mediated cognitive dysfunction, most of these pharmaceutical agents are associated with various undesirable side-effects and their long-term benefits are therefore in doubt. Early evidence exists to support the use of traditional Chinese medicine (TCM) interventions, which tend to have minimal toxicity and side-effects. More importantly, these TCM interventions appear to offer significant effects in reducing DM-related complications beyond blood glucose control. However, more research is needed to further validate these claims and to explore their relevant mechanisms of action. The aims of this paper are (1) to provide an updated overview on the association between DM and cognitive dysfunction and (2) to review the scientific evidence underpinning the use of TCM interventions for the treatment and prevention of DM-induced cognitive dysfunction and dementia.

SUMMARY OF CHROMATOGRAPHIC ANALYSIS METHODS OF ANTI DIABETIC GLIFLOZINS EMPAGLIFLOZIN, CANAGLIFLOZIN AND DAPAGLIFLOZIN

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 80-83
Author(s):  
W. A Zaghary ◽  
S. Mowaka ◽  
M. S. Hendy ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Biological Samples ◽  
Chromatographic Analysis ◽  
Dosage Forms ◽  
Drug Control ◽  
Bioanalytical Assays

Sodium glucose co-transporter-2 (SGLT-2) inhibitors are relatively new developed effective oral anti-diabetic agents used in treatment of type 2 Diabetes Mellitus. They present either alone or in combination with other ant diabetic agents such as linagliptin, Saxagliptin and metformin. Therefore, the necessity to explore and compare the existing analytical and bioanalytical assays used for determination of such drugs either single or in combination is crucial. Many methods were reported in the literature for the bio-analysis and analysis of three novel gliflozins with applying the method on different dosage forms and different chemical and biological samples. Furthermore, this review offered an overview of different methods used for determination of every drug alone in a tabulated comparative way. Moreover, the present review emphasized the most common stability indicating assays to be of interest to the analysts in the area of drug control.

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