Glycinebetaine mitigates drought stress-induced oxidative damage in pears

Glycinebetaine (GB) is an osmoprotectant found in plants under environmental stresses that incorporates drought and is associated with drought tolerance in several plants, such as the woody pear. However, how GB improves drought tolerance in pears remains unclear. In the current study, we explored the mechanism by which GB enhances drought tolerance of whole pear plants (Pyrus bretschneideri Redh. cv. Suli) supplied with exogenous GB. The results showed that on the sixth day after withholding water, levels of O2·−, H2O2, malonaldehyde (MDA) and electrolyte leakage in the leaves were substantially increased by 143%, 38%, 134% and 155%, respectively. Exogenous GB treatment was substantially reduced O2·−, H2O2, MDA and electrolyte leakage (38%, 24%, 38% and 36%, respectively) in drought-stressed leaves. Furthermore, exogenous GB induced considerably higher antioxidant enzyme activity in dry-stressed leaves than drought-stressed treatment alone on the sixth day after withholding water, such as superoxide dismutase (SOD) (201%) and peroxidase (POD) (127%). In addition, these GB-induced phenomena led to increased endogenous GB levels in the leaves of the GB 100 + drought and GB 500 + drought treatment groups by 30% and 78%, respectively, compared to drought treatment alone. The findings obtained were confirmed by the results of the disconnected leaf tests, in which GB contributed to a substantial increase in SOD activity and parallel dose- and time-based decreases in MDA levels. These results demonstrate that GB-conferred drought resistance in pears may be due in part to minimizing symptoms of oxidative harm incurred in response to drought by the activities of antioxidants and by reducing the build-up of ROS and lipid peroxidation.

Download Full-text

Responses of physiochemical attributes of kinnow budded on Jatti Khatti to triazole treatment under salinity

Acta Agronomica Hungarica ◽

10.1556/aagr.60.2012.4.13 ◽

2012 ◽

Vol 60 (4) ◽

pp. 433-447

Author(s):

V. Kakade ◽

A. Dubey ◽

O. Awasthi ◽

A. Dahuja

Keyword(s):

Superoxide Dismutase ◽

Enzyme Activity ◽

Nacl Stress ◽

Antioxidant Enzyme Activity ◽

Proline Content ◽

Nutrient Accumulation ◽

Sod Activity ◽

Citrus Jambhiri ◽

Leaf Tissues ◽

Striking Effect

The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accumulation of kinnow (Citrus nobilis × C. deliciosa) budded on Citrus jambhiri Lush rootstocks (var. Jatti Khatti) to triazole compounds was studied under NaCl stress. Eighteen-month-old budded kinnow plants were irrigated with 0 or 50 mM NaCl with or without triazole compounds. Paclobutrazol (PBZ) at 100 mg 1−1 and triazole (TRZ), triadimefon (TDM) and hexaconazole (HEX) at 20 mg l−1 were applied by drenching the soil one week prior to NaCl application. PBZ, TRZ and TDM were equally effective in preventing chlorophyll degradation under NaCl stress. The improvement in catalase (CAT) activity was greater in TRZ- and TDM-treated plants under stress, while for superoxide dismutase (SOD) activity, TRZ, PBZ and TDM were found to be equally effective. The peroxidase (POX) activity increased more in TDM-treated plants. The application of TDM and HEX had a more pronounced effect on the upregulation of proline content under stress. Furthermore, triazole compounds increased the accumulation of K+ and Ca2+ ions and inhibited that of Na+ and Cl−, resulting in an improvement in the K+/Na+ and K+/Cl− ratios. For PBZ-treated plants, the K+/Na+ and K+/Cl− ratios in leaf tissues increased 2.4- and 2.1-fold compared to plants treated with NaCl alone, while HEX had a more striking effect on Ca2+ upregulation and significantly (1.5-fold) increased leaf Ca2+ under stress.

Download Full-text

Stressed Response of Submerged Elodea nattalii under Eutrophication Growth Conditions

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.610-613.229 ◽

2012 ◽

Vol 610-613 ◽

pp. 229-234

Author(s):

Han Feng Xiong ◽

Qi Ling Tan

Keyword(s):

Superoxide Dismutase ◽

Enzyme Activity ◽

Aquatic Plants ◽

Nutrient Concentration ◽

Growth Conditions ◽

Antioxidant Enzyme Activity ◽

Metabolic Responses ◽

Concentration Increase ◽

Time Period ◽

Mda Content

Eutrophication in water bodies affects the growth of aquatic plants. In this study, we conducted static experiments to better understand the metabolic responses of Elodea nattalii under eutrophication conditions. The nitrogen (N) and phosphorous (P) levels in tissue, malondiadehyde (MDA), and activities of three antioxidases (peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)) of Elodea nattalii cultured at different nutrient levels (oligotropher, mesotropher, eutropher, and hypertrophics) were investigated. The results showed that with nutrient concentration increase, N and P contents of Elodea nattalii increased. The MDA content improved with nutrient increase. the POD activity initially increased and later decreased. CAT and SOD activities decreased during the experimental time period. These finding suggested that changes in stem the increase of antioxidant enzyme activity can be served as the indicators of the response of Elodea nattalii to eutrophication conditions.

Download Full-text

Вплив хлориду кадмію на стан антиоксидантної системи у печінці щурів

Вісник Полтавської державної аграрної академії ◽

10.31210/visnyk2013.02.28 ◽

2013 ◽

pp. 102-103

Author(s):

Б. В. Гутий

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Enzyme Activity ◽

Glutathione Peroxidase ◽

Antioxidant System ◽

Cadmium Chloride

Розкрито особливості антиоксидантної системиорганізму щурів за хронічного кадмієвого токсикозу.Встановлено, що хлорид кадмію у токсичній дозісприяє зниженню активності ферментної й нефер-ментної системи антиоксидантного захисту, на щовказує зниження ферментів глутатіонпероксидази,глутатіонредуктази, супероксиддисмутази, катала-зи та відновленого глутатіону у печінці щурів. Ре-зультати досліджень вказують на те, що хронічнийкадмієвий токсикоз призводить до посиленої акти-вації процесів ліпопероксидації. The features of the antioxidant system of rats with chronic cadmium toxicosiare disclosed. It wasresearched that cadmium chloride in toxic doses reduces enzyme activity of antioxidant system, asindicated by the decrease in enzyme glutathione peroxidase, hlutationreduktazy, superoxide dismutase,catalase and restored glutathione in the liver and blood of rats. The results indicate that chroniccadmium toxicosis leads to enhanced activation of lipid peroxidation.

Download Full-text

THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i2.29594 ◽

2019 ◽

pp. 305-309

Author(s):

AFRIWARDI AFRIWARDI ◽

DELMI SULASTRI ◽

YUNIAR LESTARI ◽

DESMAWATI DESMAWATI ◽

PRIMA MINERVA

Keyword(s):

Superoxide Dismutase ◽

Enzyme Activity ◽

Fatty Acid ◽

Telomere Length ◽

Saturated Fatty Acids ◽

Cross Sectional Study ◽

Fat Intake ◽

Sod Activity ◽

Cross Sectional ◽

Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

Download Full-text

Isolation and characterization of a rat cDNA clone encoding a secreted superoxide dismutase reveals the epididymis to be a major site of its expression

Biochemical Journal ◽

10.1042/bj2930021 ◽

1993 ◽

Vol 293 (1) ◽

pp. 21-25 ◽

Cited By ~ 53

Author(s):

A C F Perry ◽

R Jones ◽

L Hall

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Enzyme Activity ◽

Sequence Analysis ◽

Cdna Clone ◽

Northern Blot ◽

Northern Blot Analysis ◽

Major Site ◽

Isolation And Characterization

Superoxide dismutase (SOD) plays a key role in combating loss of fertility of spermatozoa due to lipid peroxidation. Here we report the sequence of a cDNA encoding a secreted form of SOD isolated from a rat epididymal library. Northern-blot analysis indicates that the corresponding transcript is expressed principally in the cauda region of the epididymis, consistent with the high levels of SOD enzyme activity found in cauda-epididymidal plasma. Much lower levels of an identically sized transcript exist in all tissues examined, including placenta. PCR and subsequent sequence analysis of rat placental SOD strongly suggest that it is identical in sequence with epididymal SOD.

Download Full-text

Integrated biomarker responses of the submerged macrophyte Vallisneria spiralis via hydrological processes from Lake Poyang, China

Royal Society Open Science ◽

10.1098/rsos.180729 ◽

2018 ◽

Vol 5 (12) ◽

pp. 180729 ◽

Cited By ~ 5

Author(s):

Yong Ji ◽

Zhidong Yao ◽

Jie Zhang ◽

Xueru Wang ◽

Jixiang Luo ◽

...

Keyword(s):

Enzyme Activity ◽

Light Intensity ◽

Water Level ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Antioxidant Enzyme Activity ◽

Water Levels ◽

Antioxidant Enzyme Activities ◽

Wetland Plant ◽

Lake Poyang

Vallisneria spiralis , a widely distributed wetland plant, was used to reveal how the light intensity at the top of the plant, plant morphology and antioxidant enzyme activity respond to different hydrologic conditions from Lake Poyang, China. By designing a laboratory experiment simulating historical water levels of low, normal and high wetland plant submersion, this study aimed to elucidate the effects of different levels of flooding on growth and antioxidant enzyme activity for V. spiralis . The results showed that the plant crown light intensity of the treated group and control group (CG) first decreased and then increased along with the seasonal variation of the water level. The maximum and minimum values of the plant crown light intensity were observed in April and July, respectively. Similar to the CG, V. spiralis from the normal and low water level (LWL) groups was measured and had higher plant height growth in the flooding period from May to June, and the entire plant biomass also showed a steady growth trend in the same period. However, the plant growth of the high water level (HWL) group was lower during the whole simulation period, with negative growth in July. Antioxidant enzyme activities changed with the seasonal temperature, and the activity of the CG showed a rising trend. Compared with those of the CG, the antioxidant enzyme activities of the HWL group showed a ‘bell shaped’ trend, which was first significantly induced and then significantly inhibited. In addition, the peroxidase (POD) and catalase (CAT) activities from the LWL group in April were also significantly induced. The integrated biomarker response (IBR) index showed that a comprehensive biological index could well reflect the effects of seasonal water levels in Poyang Lake on the growth of the wetland plant V. spiralis . This study indicated that high flooding levels had the strongest negative effect on the growth and enzyme activity of the submerged plant V. spiralis .

Download Full-text