scholarly journals Increased sensitivity of heavy metal bioreporters in transporter deficient Synechocystis PCC6803 mutants

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261135
Author(s):  
Gábor Patyi ◽  
Barbara Hódi ◽  
Dániel Solymosi ◽  
Imre Vass ◽  
Péter B. Kós
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Health Hazard ◽  
Heavy Metal Concentration ◽  
Marker Genes ◽  
Synechocystis Pcc6803 ◽  
Detection And Identification ◽  
In The Wild ◽  
Increased Sensitivity ◽  
Heavy Metal Transport

The detection and identification of heavy metal contaminants are becoming increasingly important as environmental pollution causes an ever-increasing health hazard in the last decades. Bacterial heavy metal reporters, which constitute an environmentally friendly and cheap approach, offer great help in this process. Although their application has great potential in the detection of heavy metal contamination, their sensitivity still needs to be improved. In this study, we describe a simple molecular biology approach to improve the sensitivity of bacterial heavy metal biosensors. The constructs are luxAB marker genes regulated by the promoters of heavy metal exporter genes. We constructed a mutant strain lacking the cluster of genes responsible for heavy metal transport and hence achieved increased intracellular heavy metal content of the Synechocystis PCC6803 cyanobacterium. Taking advantage of this increased intracellular heavy metal concentration the Ni2+; Co2+ and Zn2+ detection limits of the constructs were three to tenfold decreased compared to the sensitivity of the same constructs in the wild-type cyanobacterium.

Distribution of heavy metals in seawater, sediment and biota of Jinhae Bay, Korea

1994 ◽  
Vol 30 (10) ◽  
pp. 173-177 ◽  
Author(s):  
Lee Chan-Won ◽  
Kwon Young-Tack
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Coastal Waters ◽  
Heavy Metal Contamination ◽  
Heavy Metal Concentration ◽  
Industrial Wastes ◽  
Subsequent Increase ◽  
Zinc Cadmium ◽  
Jinhae Bay ◽  
Copper Lead

Over the past two decades, the coastal waters of Jinhae Bay have been extensively used by coastal communities and industries for the disposal of domestic and various industrial wastes, therefore increasing the level of pollutants in coastal waters with a subsequent increase in sediments, especially of heavy metals. Specific objectives of this research are to investigate the distribution of heavy metal concentration in biota, to compare the concentrations with those in sediment and water and to relate the bioconcentration to the different heavy metals in biota obtained from several sites. Sixty one percent of heavy metals was found in particulate form during the high runoff season and 32% during the dry season. The behavior of the particulate metals after flowing in to the enclosed coastal sea is an important factor in heavy metal contamination. Copper, lead and chromium contamination of sediment was revealed at several sites. The bioconcentration factors (BCFs) of zinc, cadmium, copper, nickel, chromium and lead by the mussel (Mytilus edulis) were determined as 2,900, 2,814, 807, 423, 228 and 127 in the decreasing order, respectively. The areas located nearest to highly populated city and industries exhibited mussels with the largest accumulation of copper, lead and chromium.

Heavy metal contents of soils and plants adjacent to the Hume Highway near Marulan, New South Wales

1975 ◽  
Vol 15 (74) ◽  
pp. 414 ◽  
Author(s):  
DJ David ◽  
CH Williams
Keyword(s):  
Heavy Metal ◽  
Zinc Oxide ◽  
Heavy Metal Contamination ◽  
New South ◽  
Health Hazard ◽  
The United States ◽  
South Wales ◽  
Metal Contents ◽  
Cadmium And Lead ◽  
Relative Purity

HCL-extractable zinc, lead, cadmium and copper in soils as well as concentrations of these elements in bracken (Pteridium esculentum) were found to decrease along four leeward traverses at right angles to the Hume Highway near Marulan. Similar results were obtained for the above-ground portions of rye corn (Secale cereale) grown in a glasshouse on soils collected along one of these traverses. Zinc accession is similar to, but cadmium and lead accessions are considerably lower than, those observed by other workers in the United States probably owing in the case of cadmium to the use of purer zinc oxide in Australian tyre manufacture and in the case of lead to smaller cars and a smaller proportion of vehicles operating on leaded fuel. Zinc and cadmium analyses of Australian and foreign tyres confirmed the relative purity of the zinc oxide used in the manufacture of tyres in Australia. No serious health hazard appears to arise from heavy metal contamination of roadside soils and vegetation by vehicular traffic on the Hume Highway.

ASSESSMENT OF HEAVY METAL CONTAMINATION OF SOIL SAMPLES COLLECTED FROM THE AREA AROUND AL-MARQUB CEMENT PLANT

2019 ◽  
Vol 2 (2) ◽  
pp. 1-9
Author(s):  
Dr. Salem M. ELNakeib ◽  
Dr. Fathi K. Elyaagubi ◽  
Dr. Mohamed A. Alrabib ◽  
Eng. Afaf Abouzed ◽  
Eng. Hanan Saleh Wanis
Keyword(s):  
Heavy Metal ◽  
Soil Properties ◽  
Heavy Metal Contamination ◽  
Calcium Content ◽  
Heavy Metal Concentration ◽  
Soil Samples ◽  
Cement Dust ◽  
Physiochemical Properties ◽  
Cement Factory ◽  
High Concentrations

Soil samples were collected from and around Almarqub cement factory, AL-Khums city, Libya. These samples were collected from four different sites M1 (inside the factory), M2 (150 m from the factory), M3 (350 m from the factory) and M4 (60 km away from the factory as the control samples. The study was conducted to determine the heavy metal concentration in the soil. Organic matter, pH and water content value were determined according to the method described by Chaturvedi and Sankar (2006). Metals were determined by Atomic Absorption Spectrophotometer. The results obtained for the examined physiochemical properties of soil in the area studied prove that cement dust from the Almarqub cement factory has had a significant impact on the soil. The affected soil properties are pH and total calcium content. These properties were found to be higher than those in similar soils from the same area unpolluted. The increase of soil pH in the same area may be a result of precipitation of cement dust over the years. Metal uptake from cement to soil and plants. Metals determined in contamination soil indicated high concentrations in M1 inside cement factory compared to soil samples as control. Results of the analysis have shown that there are signs of slight impact of soil properties arising from the cement dust on the soils, especially at location inside the factory. Recommendations were offered to monitor the dust falling on the soils through trapping and utilizing the dust emissions of cement.

scholarly journals Heavy Metal Contain Cu and Cd on the Mullet in the estuary of Donan River, Cilacap, Central Java

2017 ◽  
Vol 20 (1) ◽  
pp. 18 ◽  
Author(s):  
Yudha Prastyo ◽  
Djamar T.F Lumban Batu ◽  
Sulistiono Sulistiono
Keyword(s):  
Heavy Metal ◽  
Body Weight ◽  
Atomic Absorption ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Heavy Metal Concentration ◽  
Fish Muscle ◽  
Aquatic Area ◽  
Central Java ◽  
Safety Limit

The estuary of Donan River is an aquatic area that commonly utilized for some activities such as industrial, fisheries and domestic activities. The effluents of many activities may be cause heavy metal contamination for water enivornemant and organism live in the water. The aim of this research was to analyse heavy metal contain Cu and Cd on the mullet (Chelon subviridis) muscle caught at estuary of Donan River, and to determine the safety limit for community consumption. Sampling had been carried out for 6 months, from August 2015 to January 2016. Heavy metal concentration was measured by AAS (Atomic Absorption Spectrophotometer). Result of the study shows that heavy metal Cu and Cd in the fish muscle were 0.5009-2.6021 mg/kg and 0.0165-0.2307 mg/kg, respectively that were higher than safety limit.  Based on the study, the safety limit for consumption is 151.72 g meat/week (for adult 50 kg body weight) and 45.52<br />g meat/week (for child 15 kg body weight).

scholarly journals ASSESSMENT OF HEAVY METAL CONCENTRATION IN VEGETABLES DRIED ALONG FUNTUA-DANJA HIGHWAY KATSINA STATE, NIGERIA

2021 ◽  
Vol 5 (1) ◽  
pp. 467-471
Author(s):  
U. M. Kankara ◽  
Rabiu Nasiru ◽  
Nuraddeen Nasiru Garba ◽  
Jamila Musa Kankara ◽  
Umar Musa Kankara
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Atomic Absorption Spectrometry ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Absorption Spectrometry ◽  
Heavy Metal Concentration ◽  
Metal Exposure ◽  
Heavy Metal Exposure ◽  
Concentration Levels

One of the major routes of heavy metal exposure to humans is via the consumption of vegetable. The study assessed the contribution of automobile emission on the concentration levels of heavy metals in some of commonly consumed vegetables in Katsina state, Nigeria using Atomic Absorption Spectrometry (AAS). Fresh and dried samples of five (5) commonly consumed vegetables were obtained from Danja-Funtua highway road, 0.5 g of each sample was measured into a clean dried beaker and 10 ml of acidic mixture of HNO3/HClO4 in ratio 2:1 was added to the sample for digestion. The heavy metals concentration in this study ranges from 0.6 – 75.5mg/kg, 0.31 – 1.2mg/kg 278 – 1470mg/kg, 0.4 – 36.3mg/kg and 28 – 65 mg/kg for Pb, Cd, Fe, Ni and Cu respectively. Levels of heavy metals were all found to be above the acceptable limits indicating that inhabitants are at risk of heavy metal contamination

Spectral Sensitivity of Heavy Metal Concentration in Mine Soils: A case study of Gagok mine, South Korea

2020 ◽  
Author(s):  
Hyesu Kim ◽  
Jaehyung Yu ◽  
Jieun Kim ◽  
Sangmo Koh ◽  
Bumhan Lee
Keyword(s):  
Heavy Metal ◽  
Metal Concentration ◽  
Mineral Composition ◽  
Clay Mineral ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Heavy Metal Concentration ◽  
Mine Soils ◽  
Silicate Clay ◽  
Silicate Carbonate

&lt;p&gt;&lt;span&gt;&lt;span&gt;&amp;#160;Heavy metal contamination in soils of mining area accompanies contaminations in drainage and vegetation. The traditional soil survey methods for heavy metal contamination requires significant investment of time, labor, and money although the methods can drive accurate assessment. Moreover, the point-based survey is problematic to understand spatial distribution with limited sample numbers. Remote sensing approaches may provide alternative solutions to understand spatial distribution of contamination with minimal investment of time and labor. It is very critical to understand spectral signals associated with heavy metal concentration to apply the phenomenon to remote sensing approaches. This study investigated spectral signals associated with heavy metal concentration in mine soils based on chemical analysis, mineral composition analysis, and spectral analysis. The study site is a Pb-Zn skarn ore deposit located in Gangwon province, South Korea, where the ore was formed by granite intrusion to slate and limestone. The chemical analysis revealed that the soils are contaminated with heavy metals. Mineral composition classified the soils into two time with silicate/clay mineral group and silicate/carbonate/clay mineral groups. Spectral analysis showed that sensitive spectral bands to heavy metal concentration is located in SWIR region for silicate/clay mineral group and VNIR region for silicate/carbonate/clay mineral group. It reveals that mineral composition and geochemical reaction play an important role in spectral signals manifested by heavy metal concentration. &lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;

scholarly journals Phosphorylation of a malate transporter promotes malate excretion and reduces cadmium uptake in apple

2020 ◽  
Vol 71 (12) ◽  
pp. 3437-3449
Author(s):  
Qi-Jun Ma ◽  
Mei-Hong Sun ◽  
Jing Lu ◽  
Da-Gang Hu ◽  
Hui Kang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Cadmium Toxicity ◽  
Health Hazard ◽  
Membrane System ◽  
Heavy Metal Stress ◽  
Bimolecular Fluorescence Complementation ◽  
Cadmium Uptake ◽  
Dependent Manner ◽  
Malate Transporter

Abstract Heavy metal contamination is a major environmental and human health hazard in many areas of the world. Organic acids sequester heavy metals and protect plant roots from the effects of toxicity; however, it is largely unknown how these acids are regulated in response to heavy metal stress. Here, protein kinase SOS2L1 from apple was functionally characterized. MdSOS2L1 was found to be involved in the regulation of malate excretion, and to inhibit cadmium uptake into roots. Using the DUAL membrane system in a screen of an apple cDNA library with MdSOS2L1 as bait, a malate transporter, MdALMT14, was identified as an interactor. Bimolecular fluorescence complementation, pull-down, and co-immunoprecipitation assays further indicated the interaction of the two proteins. Transgenic analyses showed that MdSOS2L1 is required for cadmium-induced phosphorylation at the Ser358 site of MdALMT14, a modification that enhanced the stability of the MdALMT14 protein. MdSOS2L1 was also shown to enhance cadmium tolerance in an MdALMT14-dependent manner. This study sheds light on the roles of the MdSOS2L1–MdALMT14 complex in physiological responses to cadmium toxicity.

scholarly journals Heavy Metal Contamination of Natural Foods Is a Serious Health Issue: A Review

2021 ◽  
Vol 14 (1) ◽  
pp. 161
Author(s):  
Naveed Munir ◽  
Muhammad Jahangeer ◽  
Abdelhakim Bouyahya ◽  
Nasreddine El Omari ◽  
Rokia Ghchime ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Human Health ◽  
Metal Contamination ◽  
Fruits And Vegetables ◽  
Heavy Metal Contamination ◽  
Heavy Metal Concentration ◽  
Living Cells ◽  
Open Access Journals ◽  
Natural Foods

Heavy metals play an important role in the homeostasis of living cells. However, these elements induce several adverse environmental effects and toxicities, and therefore seriously affect living cells and organisms. In recent years, some heavy metal pollutants have been reported to cause harmful effects on crop quality, and thus affect both food security and human health. For example, chromium, cadmium, copper, lead, and mercury were detected in natural foods. Evidence suggests that these elements are environmental contaminants in natural foods. Consequently, this review highlights the risks of heavy metal contamination of the soil and food crops, and their impact on human health. The data were retrieved from different databases such as Science Direct, PubMed, Google scholar, and the Directory of Open Access Journals. Results show that vegetable and fruit crops grown in polluted soil accumulate higher levels of heavy metals than crops grown in unpolluted soil. Moreover, heavy metals in water, air, and soil can reduce the benefits of eating fruits and vegetables. A healthy diet requires a rational consumption of foods. Physical, chemical, and biological processes have been developed to reduce heavy metal concentration and bioavailability to reduce heavy metal aggregation in the ecosystem. However, mechanisms by which these heavy metals exhibit their action on human health are not well elucidated. In addition, the positive and negative effects of heavy metals are not very well established, suggesting the need for further investigation.

scholarly journals Potential of Ricinus communis L. For Removal of Heavy Metal in Contaminated Soil

2021 ◽  
Vol 25 (3) ◽  
pp. 371-376
Author(s):  
O.O. Akintola ◽  
I.O. Abiola ◽  
E.K. Abodunrin ◽  
O.S. Olokeogun ◽  
A.A. Ekaun ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Ricinus Communis ◽  
Translocation Factor ◽  
Heavy Metal Concentration ◽  
Heavy Metal Accumulation ◽  
Ecological Problem ◽  
Control Soil ◽  
Before And After

Heavy metal contamination has become a serious ecological problem due to its toxic effects on soils, plants and human. Experimental study was conducted on dumpsite soil to assess the potential of Ricinus communis to accumulate heavy metals from the soil using bioconcentration (BCF) and tanslocation factors (TF). Heavy metals concentration (mg/kg) in dumpsite and control soil before planting were Mn (50.68- 220.08); Zn(29.01- 135.56); Cu (8.92- 86.88), Pb (5.88-48.86), Ni (3.01-7.99) and Co (1.78-6.88) while the concentration in soils after planting were Mn(29.89- 135.21); Zn (15.11-88.21); Cu (3.89-50.22), pb (3.68-31.56), Ni (1.22-3.56) and Co (0.67-2.68) in Mg/kg. Ricinus communis showed BCF greater than 1 for Ni and Co and less than 1 for Mn, Cu, Zn and Pb while TF is greater than 1 for all the determined heavy metals. The dumpsite soils have higher heavy metal concentration than the control soil. The levels of heavy metals concentration in soils and plants are in the order of Mn> Zn> Cu> Pb> Ni. Significant reduction (P<0.05) was observed in the heavy metal concentrations in the soils before and after planting indicating their accumulation in the plants. Results of this study indicate the accumulation of heavy metals in Ricinus communis plants and its potential for effective removal of Cu, Zn, Pb, Ni, Co and Mn from the dumpsite soils. Keywords: Heavy metal accumulation, Ricinus communis, Dumpsite soil, Translocation factor, Remediation

Sign in / Sign up

Export Citation Format

Share Document