scholarly journals The therapeutic efficacy of Aloe vera gel ointment on staphylococcal pyoderma in dogs

2020 ◽  
Vol 13 (11) ◽  
pp. 2371-2380
Author(s):  
Ahmed Kamr ◽  
Ali Arbaga ◽  
Amanallah El-Bahrawy ◽  
Ahmed Elsify ◽  
Hadeer Khaled ◽  
...  
Keyword(s):  
Therapeutic Efficacy ◽  
Aloe Vera ◽  
Inhibition Zone ◽  
Complete Healing ◽  
Aloe Barbadensis ◽  
Local Breed ◽  
Aloe Vera Gel ◽  
Antioxidant Parameters ◽  
Anti Inflammatory ◽  
Factor Α

Background and Aim: Staphylococcus pyoderma is a common problem in dogs that need a novel treatment rather than antibiotic therapy. The aim of this study was to investigate the therapeutic efficacy, anti-inflammatory, and antioxidative properties of Aloe vera (Aloe barbadensis) gel ointment on dogs' Staphylococcus pyoderma compared to gentamicin ointment. Materials and Methods: The inhibition zone of A. vera extract 20% and 40% and gentamicin 1% against Staphylococcus aureus was determined on well diffusion agar. Twenty Baladi local breed dogs were used as control negative group before intradermal inoculation with 105 CFU S. aureus. The animals were classified into four equal groups, control positive group without treatment (n=5), treated groups by 20% A. vera gel ointment (n=5), 40% A. vera gel ointment (n=5), and gentamicin ointment 1% (n=5). Topical application of A. vera and gentamicin ointments was carried out twice daily for 2 weeks until complete healing of dogs' pyoderma. Clinical evaluation was recorded. Inflammatory, oxidant, and antioxidant parameters were measured in serum. Results: The inhibition zone of A. vera extracts 20% and 40% was 19 mm and 23 mm, respectively, while gentamicin 1% was 18 mm. The half maximal inhibitory concentration (of A. vera 20% and 40% were 13.70 with R2=0.98. Dogs' pyoderma treated with A. vera gel ointment 20% and 40% were more likely to have low haptoglobin and tumor necrosis factor-α concentrations than gentamicin 1% ([odds ratio [OR]=4.6; 95% confidence interval [CI]=1.31-17.40; p<0.05]; [OR=5.2; 95% CI=1.04-22.30; p<0.05]), respectively. Conclusion: It seems evident that A. vera has therapeutic effect, antibacterial, and anti-inflammatory effects against dogs' staphylococcal pyoderma than gentamicin that would support its further use rather than antibiotics in one health arena.

scholarly journals An Innovation for Retarded Healing Process of a Chronic Ulcer by Aloe Vera Gel Treatment

2016 ◽  
Vol 16 (2) ◽  
pp. 45 ◽  
Author(s):  
Majid Avijgan ◽  
Mahtab Avijgan ◽  
Atousa Hakamifard ◽  
Niloufar Razavi
Keyword(s):  
Potassium Permanganate ◽  
Conventional Treatment ◽  
Healing Process ◽  
Aloe Vera ◽  
Complete Healing ◽  
Response To Therapy ◽  
Chronic Ulcer ◽  
Alternative Methods ◽  
Aloe Vera Gel ◽  
Gel Treatment

In some cases of chronic ulcers of soft tissue cannot be treated by conventional treatment methods. In recent years some alternative methods have been proposed for treatment of such ulcers on the basis of Traditional Iranian (Persian) Medicine. This reports a case of chronic ulcer treatment by using <em>Aloe vera</em> gel. The ulcer was caused by car accident and in spite of using conventional treatment the injury remained for 60 days without appropriate response to therapy. Because of stopping in healing process by Aloe vera gel, this was discontinued and potassium permanganate has been used. After drying the ulcer using the alternative method of <em>Aloe vera</em> gel and potassium permanganate together has been applied and then the <em>Aloe vera</em> gel was used until the complete healing of ulcer. The delayed healing process of <em>Aloe vera</em> gel raise the question whether it is solely effective in complete healing of chronic ulcer or requires adjuvant therapies. Although <em>Aloe vera</em> is effective in healing process of chronic ulcer but sometimes it may be ineffective so that we are obliged to use potassium permanganate as an adjuvant drug which is presented by this case report.

scholarly journals Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

2020 ◽  
Vol 10 (2) ◽  
pp. 106
Author(s):  
Dian Meididewi Nuraini ◽  
Morsid Andityas ◽  
Adi Paramarta ◽  
Nur Rohman Najib ◽  
Agustina Dwi Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Drinking Water ◽  
Inhibitory Activity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Test ◽  
Aloe Barbadensis ◽  
E Coli

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%.  Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity   Abstrak  Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%.  Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

scholarly journals Effect of different extracts of Aloe Vera gel (Aloe Barbadensis) on blood glucose level of alloxan induced hyperglycaemic mice

2016 ◽  
Vol 44 (2) ◽  
pp. 61-66
Author(s):  
Shammin Haque ◽  
Ferdous Ara ◽  
Md Jalaluddin Iqbal ◽  
Sheikh Nazrul Islam
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Cost Effective ◽  
Aloe Barbadensis ◽  
Aloe Vera Gel ◽  
Dried Extract

This study was conducted to evaluate the effect of different extracts of Aloe Vera gel in alloxan induced hyperglycaemic mice.Three different extracts of Aloe Vera gel (dried extract, ethanolic extract and fresh raw extract) were orally administered at 300 mg/kg body weight for 28 days. The fasting blood glucose level was estimated both in normal and alloxan induced hyperglycaemic mice. It was found that, when compared with the control, there was a significant reduction in blood glucose level in all three experimental groups. Ethanolic and fresh raw gel extracts were more effective than the dried extract. The extracts produced similar results when compared with gliclazide. It can be concluded that, the administration of Aloe Vera gel extract significantly decreases blood glucose level in hyperglycaemic mice. Aloe Vera gel can therefore be a natural remedy and a cost effective resource for the management of diabetes.Bangladesh Med J. 2015 May; 44 (2): 61-66

A systematic Review of Aloe vera and its Properties

2021 ◽  
pp. 257-259
Author(s):  
Farha Fatma ◽  
Anil Kumar
Keyword(s):  
Systematic Review ◽  
Medicinal Plant ◽  
Review Paper ◽  
Therapeutic Potential ◽  
Aloe Vera ◽  
Paper Properties ◽  
Beneficial Effects ◽  
Aloe Vera Gel ◽  
Anti Inflammatory ◽  
Pharmaceutical Uses

Aloe vera is one of the oldest medicinal plant ever known, commonly known as Ghrit Kumari. It is well known for its therapeutic potential. Some of its beneficial effects include anticancer, hypoglycemic, antifungal, anti-inflammatory, burn healing properties. Phytochemistry of Aloe vera gel has revealed presence of bioactive chemicals. In this review paper, properties of Aloe vera, its composition, its beneficial effects and pharmaceutical uses, its application in foods and cosmetics etc. have been summarized.

scholarly journals Chemical Composition, Antibacterial, Enzyme-Inhibitory, and Anti-Inflammatory Activities of Essential Oil from Hedychiumpuerense Rhizome

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2506
Author(s):  
Yi Hong ◽  
Xiongli Liu ◽  
Huijuan Wang ◽  
Min Zhang ◽  
Minyi Tian
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Minimal Bactericidal Concentration ◽  
Edible Plant ◽  
Anti Inflammatory ◽  
Factor Α

Hedychium puerense, a perennial rhizomatous herb, is used as an ornamental, medicinal, and edible plant in Yunnan Province, China. Essential oils from Hedychium plants are widely used in perfumes and traditional medicine, but there are no studies on the constituents and bioactivities of H. puerense essential oil (EO). Therefore, this study was designed to explore the chemical composition, antibacterial, enzyme-inhibitory, and anti-inflammatory activities of H. puerense rhizome EO. The gas chromatography with flame ionization or mass selective detection (GC-FID/MS) results indicated that H. puerense EO was mainly composed of linalool (26.5%), β-pinene (18.6%), γ-terpinene (12.1%), terpinen-4-ol (7.7%), α-pinene (5.8%), sabinene (4.9%), E-nerolidol (4.1%), and p-cymene (3.6%). For biological activities, H. puerense EO displayed broad-spectrum antibacterial properties against Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, and Escherichia coli with diameter of inhibition zone (DIZ) values ranging from 7.44 to 10.30 mm, a minimal inhibitory concentration (MIC) of 3.13–6.25 mg/m), and a minimal bactericidal concentration (MBC) of 3.13–12.50 mg/mL. Moreover, the EO significantly inhibited acetylcholinesterase (AChE) (IC50 = 0.94 ± 0.02 mg/mL) and butyrylcholinesterase (BChE) (IC50 = 1.32 ± 0.06 mg/mL) activities, and exhibited a moderate inhibitory effect on α-glucosidase (IC50 = 5.42 ± 0.32 mg/mL) and tyrosinase (IC50 = 3.23 ± 0.21 mg/mL). Furthermore, the EO significantly suppressed the secretion of the pro-inflammatory mediator, nitric oxide (NO) (99.23 ± 0.26%), cytokines tumor necrosis factor-α (TNF-α) (97.14 ± 0.11%), and interleukin-6 (IL-6) (82.42 ± 0.16%) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 250 μg/mL without cytotoxicity. Hence, H. puerense EO can be considered a bioactive, natural product that has great potential for utilization in the fields of food, cosmetics, and pharmaceutics.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL LIDAH BUAYA (Aloe vera) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 17 (2) ◽  
pp. 65-70
Author(s):  
Mia Rahardjo ◽  
Eko Budi Koendhori ◽  
Yuani Setiawati
Keyword(s):  
Staphylococcus Aureus ◽  
Active Compound ◽  
Enzymatic Reaction ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Dilution Method ◽  
Minimal Amount ◽  
Aloe Vera Gel

Abstrak. Staphylococcus aureus merupakan salah satu flora normal pada kulit, membran mukosa, orofaring, saluran pencernaan dan vagina yang berpotensi menjadi patogen. Pertumbuhan S. aureus yang berlebihan dapat menimbulkan infeksi yang serius baik di manusia atau hewan. Dan sekarang, beberapa S. aureus dikabarkan telah resisten terhadap antibiotik karena proses mutasi. Berdasarkan hal tersebut, penulis mencoba memberi alternatif pengobatan dengan memanfaatkan ekstrak etanol gel Aloe vera yang menurut beberapa penulis lain, gel Aloe vera mengandung antraquinone, tannin, polysaccharide, flavonoid, and saponin yang bersifat sebagai antibakteri. Jenis penelitian ini adalah eksperimental laboratorium dengan metode difusi dan dilusi. Penelitian ini menggunakan konsentrasi 100%, 75%, 50%, 25%, dan 0% pada metode difusi. Sementara itu metode dilusi menggunakan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625% kontrol positif (+), dan kontrol negatif (-).Dari pengamatan hasil penelitian, tidak didapatkan zona inhibisi pada metode difusi serta tidak dapat ditentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) terhadap pertumbuhan Staphylococcus aureus. Hal ini terkait dengan rendahnya senyawa aktif yang digunakan di sampel gel Aloe vera dalam penelitian ini akibat pengaruh dari faktor lingkungan, perbedaan usia tanaman dengan literatur awal, proses degradasi dan reaksi enzimatik, adanya perbedaan metode ekstraksi, serta proses oksidasi saat terpapar oleh udara. Berdasarkan hal tersebut, dapat disimpulkan bahwa aktivitas antibakteri dari ekstrak etanol gel Aloe vera terhadap Staphylococcus aureus tidak dapat ditentukandengan metode difusi dan metode dilusi. (JKS 2017; 2: 65-71) Kata Kunci : Gel lidah buaya (Aloe vera), Staphylococcus aureus, antibakteri, metode difusi dan dilusi.  Abstract. Staphylococcus aureus is one of the normal flora in human skin, mucous membrane, oropharynx, gastrointestinal tract, and vagina which potentially becomes a pathogen. The excessive growth of S. aureus can cause many serious infection whether in human or animal. And nowadays, some of S.aureus have become resistant to antibiotic caused by its mutation. According to that case, researcher try to find an alternative solution by using Aloe vera gel ethanol extract that some other researchers say it contains antraquinone, tannin, polysaccharide, flavonoid, and saponin as anti bacterial compound. This research aimed to find out the effectiveness of Aloe vera gel ethanol extract in inhibiting Staphylococcus aureus.This research is designed as an laboratorium experimental with difusion and dilusion method. Test performed with using 100%, 75%, 50%, 25%, and 0% concentration in difusion method and using 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625% concentration , positive control (+) and negative control (-) in dilution method. There is no inhibition zone in difusion method, also no minimum inhibitory concentration and no bactericidal concentration can be seen in dilution method that inhibit the growth of Staphylococcus aureus. This result might be related to the minimal amount of active compound in this sample, that is taken from Aloe vera gel. The amount of active compound can be influenced by the environment, difference in Aloe’s age, degradation process and enzymatic reaction, difference in extraction method and also influenced by oxidation process when it’s exposed to air. Based on the results, anti bacterial activity of Aloe vera gel ethanol extract towards Staphylococcus aureus can not be determined in difusion and dilution method.  (JKS 2017; 2: 65-71) Key words : Aloe vera gel, Staphylococcus aureus, antibacterial, difusion and dilusion method

scholarly journals The Production of Chitosan from Shrimp Shell Waste and Its Formulation in Patch DosageForm Combined with Aloe vera Extract as Antiinfection Agent

2017 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Haeriah Haeriah ◽  
Muhammad Rahmatullah ◽  
Andi Indardaya ◽  
Emilia Utomo ◽  
Novianti Novianti ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Base Material ◽  
Inhibition Zone ◽  
Wound Dressings ◽  
Aloe Vera Gel ◽  
Shrimp Shell ◽  
Shell Waste ◽  
Percentage Yield ◽  
Shrimp Shell Waste

Chitosan can be obtained from chitin isolated from shrimp waste through the process of deacetylation of chitin. Chitosan can be used as a patch base material because of its biocompatibility and biodegradability, and has antibacterial activity. The combination of Chitosan and Aloe vera extracts may be useful in patch dosage forms as wound dressings that have antiinfective activity. The research methods was laboratory experimental which consists of : chitosan was obtained from shell waste sequentially by deproteinisation, demineralisation, and deacetylation processes, and analyzed its characteristic, respectivel; Aloe vera gel was extraction by maceration methods using ethanol as solvent; patches were formulated using 2 % chitosan in 1.5 % glacial acetic acid,  1.6 %  Aloe vera extracts and 10 % glycerin and evaluated its physical propoperties,  skin iritation test, and antibacterial test against S.aureus. The results showed that percentage yield of chitosan was 32.67%, with characteristics; Its viscosity 93.33 cps, pH 4, 30 % deacetylation degree.  Percentage yield of Aloe vera  extract was 22.73%, containing acemannan, and had antibacterial activity against S.aureus.  The result of patch evaluation showed that the thickness of patch was 0.45 mm, 114 times folding endurance, moisture uptake of 45.03%, moisture content of 23.64%, safe to use, and  diameter of inhibition zone was 6.39 mm against S.aureus. So, combination of Aloe vera and Chitosan from shrimp shell as patch may use as antiinfection wound healing.  Keywords : Aloe vera L.; chitosan; wound dressing; patch; shrimp shell waste

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