scholarly journals Comparative occurrence and antibiogram of extended-spectrum β-lactamase-producing Escherichia coli among post-weaned calves and lactating cows from smallholder dairy farms in a parallel animal husbandry area

2021 ◽  
pp. 1311-1318
Author(s):  
Chya Vannakovida ◽  
Kannika Na Lampang ◽  
Phongsakorn Chuammitri ◽  
Veerasak Punyapornwithaya ◽  
Khwanchai Kreausukon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Animal Husbandry ◽  
Dairy Farms ◽  
Fecal Samples ◽  
E Coli ◽  
Zone Diameter ◽  
Lactating Cows ◽  
Extended Spectrum ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

Background and Aim: Inappropriate overuse of antimicrobials might be associated with the spreading of antimicrobial-resistant bacteria in animal-based food products. Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli have been recognized as an emerging global problem in a One Health approach. This study aimed to assess the occurrence and antimicrobial-susceptible profiles of ESBL-producing E. coli among post-weaned calves and lactating cows in a parallel animal husbandry area. Materials and Methods: Seventy-two pool fecal samples were collected from 36 smallholder dairy farms registered in Ban Hong Dairy Cooperatives, Lamphun Province, Thailand. Pre-enriched fecal samples were cultured in MacConkey agar supplemented with cefotaxime. The potential E. coli isolates were identified by not only biochemical tests but also polymerase chain reaction assay of the 16S rRNA gene. ESBL production was confirmed by the combination disk test. Antimicrobial susceptibility testing was performed by the Kirby–Bauer disk diffusion method. Results: The occurrence of ESBL-producing E. coli at the farm level was 80.56%. The different phenotypic antibiogram of ESBL-producing E. coli was observed among post-weaned calf and lactating cow specimens. The most frequent resistance patterns of ESBL-producing isolates from both groups were amoxicillin-ceftiofur-cephalexin-cephalothin-cloxacillin-streptomycin-oxytetracycline-sulfamethoxazole/trimethoprim. For the median zone diameter, enrofloxacin-resistant isolates with narrow zone diameter values from lactating cow specimens were particularly more than post-weaned calf specimens (p<0.05). Conclusion: These findings revealed the dynamic changes in ESBL-producing E. coli from calves and lactating cows in Lamphun Province, posing the inevitability to prevent bacterial transmission and optimize antimicrobial therapy in dairy farming.

scholarly journals Detection of antimicrobial resistance genes of carbapenem-resistant Enterobacteriaceae in Escherichia coli isolated from the water supply of smallholder dairy farms in Saraburi and Maha Sarakham, Thailand

2020 ◽  
Vol 6 (1) ◽  
pp. 1-5
Author(s):  
Natapol Pumipuntu ◽  
Sangkom Pumipuntu
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Water Samples ◽  
Dairy Farms ◽  
Drug Resistant ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae ◽  
Smallholder Dairy Farms

Background and Aim: The problem of antimicrobial resistance of bacteria in both humans and animals is an important public health concern globally, which is likely to increase, including in Thailand, where carbapenem-resistant Enterobacteriaceae (CRE), such as Escherichia coli, are of particular concern. They are pathogens found in the gastrointestinal tract of humans and other animals as well as in the environment. They may cause opportunistic infection and are often resistant to antibiotics in various fields especially in animal husbandry, such as pets or livestock farms. This study aimed to investigate the occurrence of carbapenem-resistant E. coli from water samples of smallholder dairy farms in Saraburi and Maha Sarakham, Thailand. Materials and Methods: Sixty-four water samples were collected from 32 dairy farms in Kaeng Khoi district, Muak Lek district, and Wang Muang district of Saraburi Province, and Kantharawichai district and Mueang district of Maha Sarakham Province, Thailand. All samples were cultured and isolated for E. coli by biochemical tests. All E. coli isolates were tested for drug susceptibility using imipenem, meropenem, and drug resistance genes of carbapenemases such as blaNDM, blaIMP, and blaOXA48 of drug-resistant E. coli isolates detected by polymerase chain reaction (PCR) technique. Results: A total of 182 E. coli isolates were found (140 and 42 isolates from Saraburi and Maha Sarakham, respectively). Drug sensitivity tests found that two isolates of E. coli from water in Kaeng Khoi were resistant to imipenem; therefore, the incidence of E. coli resistance to carbapenem was 1.43% of Saraburi Province. On the other hand, there was no incidence of drug-resistant E. coli in Maha Sarakham. In addition, the detection of the drug-resistant gene of E. coli in both isolates by PCR showed the expression of blaNDM. Conclusion: This study reports E. coli resistance to antimicrobial drugs on livestock farms. It can be considered to be the first report of E. coli CRE detection in a dairy farm at Saraburi, which should be the subject of further extended study.

Diet factors and subclinical laminitis score in lactating cows of smallholder dairy farms in Thailand

2013 ◽  
Vol 155 (2-3) ◽  
pp. 197-204 ◽  
Author(s):  
R. Pilachai ◽  
J.Th. Schonewille ◽  
C. Thamrongyoswittayakul ◽  
S. Aiumlamai ◽  
C. Wachirapakorn ◽  
...  
Keyword(s):  
Dairy Farms ◽  
Lactating Cows ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

scholarly journals Assessment of Performance and Some Welfare Indicators of Cows in Vietnamese Smallholder Dairy Farms

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 674
Author(s):  
Nguyen N. Bang ◽  
Nguyen V. Chanh ◽  
Nguyen X. Trach ◽  
Duong N. Khang ◽  
Ben J. Hayes ◽  
...  
Keyword(s):  
Heat Stress ◽  
Milk Yield ◽  
Body Condition Score ◽  
Dairy Farms ◽  
Point Scale ◽  
The South ◽  
Lactating Cows ◽  
The North ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

Smallholder dairy farms (SDFs) are distributed widely across lowland and highland regions in Vietnam, but data on the productivity and welfare status of these cows remains limited. This cross-sectional study was conducted to describe and compare the productivity and welfare status of SDF cows across contrasting regions. It was conducted in autumn 2017 on 32 SDFs randomly selected from four typical but contrasting dairy regions (eight SDFs per region); a south lowland, a south highland, a north lowland, and a north highland region. Each farm was visited over a 24-h period (an afternoon followed by a morning milking and adjacent husbandry activities) to collect data of individual lactating cows (n = 345) and dry cows (n = 123), which included: milk yield and concentrations, body weight (BW), body condition score (BCS, 5-point scale, 5 = very fat), inseminations per conception, and level of heat stress experienced (panting score, 4.5-point scale, 0 = no stress). The high level of heat stress (96% of lactating cows were moderate to highly heat-stressed in the afternoon), low energy corrected milk yield (15.7 kg/cow/d), low percentage of lactating cows (37.3% herd), low BW (498 and 521 kg in lactating and dry cows, respectively), and low BCS of lactating cows (2.8) were the most important productivity and welfare concerns determined and these were most serious in the south lowland. By contrast, cows in the north lowland, a relatively hot but new dairying region, performed similarly to those in the south highland; a region historically considered to be one of the most suitable for dairy cows in Vietnam due to its cool environment. This indicates the potential to mitigate heat stress through new husbandry strategies. Cows in the north highland had the highest BW (535 and 569 kg in lactating and dry cows, respectively) and the highest energy corrected milk yield (19.2 kg/cow/d). Cows in all regions were heat-stressed during the daytime, although less so in the highlands compared to the lowlands. Opportunities for research into improving the productivity and welfare of Vietnamese SDF cows are discussed.

scholarly journals Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli

2021 ◽  
Vol 9 (2) ◽  
pp. 367 ◽  
Author(s):  
Anna R. Holtmann ◽  
Diana Meemken ◽  
Anja Müller ◽  
Diana Seinige ◽  
Kathrin Büttner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Phylogenetic Groups ◽  
Fecal Samples ◽  
Wild Boars ◽  
E Coli ◽  
Extended Spectrum ◽  
Nasal Swabs ◽  
Typing Methods ◽  
Lactamase Gene

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) represent major healthcare concerns. The role of wildlife in the epidemiology of these bacteria is unclear. The purpose of this study was to determine their prevalence in wild boars in Germany and to characterize individual isolates. A total of 375 fecal samples and 439 nasal swabs were screened for the presence of ESBL-/AmpC-E. coli and MRSA, respectively. The associations of seven demographic and anthropogenic variables with the occurrence of ESBL-/AmpC-E. coli were statistically evaluated. Collected isolates were subjected to antimicrobial susceptibility testing, molecular typing methods, and gene detection by PCR and genome sequencing. ESBL-/AmpC-E. coli were detected in 22 fecal samples (5.9%) whereas no MRSA were detected. The occurrence of ESBL-/AmpC-E. coli in wild boars was significantly and positively associated with human population density. Of the 22 E. coli, 19 were confirmed as ESBL-producers and carried genes belonging to blaCTX-M group 1 or blaSHV-12. The remaining three isolates carried the AmpC-β-lactamase gene blaCMY-2. Several isolates showed additional antimicrobial resistances. All four major phylogenetic groups were represented with group B1 being the most common. This study demonstrates that wild boars can serve as a reservoir for ESBL-/AmpC-producing and multidrug-resistant E. coli.

scholarly journals Prevalence of Extended-Spectrum-β-Lactamase- and/or Carbapenemase-Producing Escherichia coli Isolated from Yellow-Legged Gulls from Barcelona, Spain

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Andrea Vergara ◽  
Cristina Pitart ◽  
Tomás Montalvo ◽  
Ignasi Roca ◽  
Sara Sabaté ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fecal Samples ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT Seventy-two (54.5%) out of 132 fecal samples from a group of yellow-legged gulls in Barcelona, Spain, were positive for Escherichia coli producing either extended-spectrum β-lactamases (ESBL) (51.5%), carbapenemase (1.5%), or cephamycinase (1.5%). The isolation of two carbapenemase-producing E. coli strains is a matter of concern.

scholarly journals Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada

2021 ◽  
Vol 8 ◽  
Author(s):  
Jonathan Massé ◽  
Hélène Lardé ◽  
John M. Fairbrother ◽  
Jean-Philippe Roy ◽  
David Francoz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Cross Sectional Study ◽  
Passive Surveillance ◽  
Sectional Study ◽  
Cross Sectional ◽  
E Coli ◽  
Extended Spectrum ◽  
High Prevalence

Antimicrobial resistance (AMR) is an important burden for public health and veterinary medicine. For Québec (Canada) dairy farms, the prevalence of AMR is mostly described using passive surveillance, which may be misleading. In addition, the presence of extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli is unknown. This observational cross-sectional study used random dairy farms (n = 101) to investigate AMR and extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli. Twenty antimicrobials were tested on E. coli isolates (n = 593) recovered from fecal samples (n = 599) from calves, cows, and the manure pit. Isolates were mostly susceptible (3% AMR or less) to the highest priority critically important antimicrobials in humans. The highest levels of AMR were to tetracycline (26%), sulfisozaxole (23%) and streptomycin (19%). The resistance genes responsible for these resistances were, respectively: tet(A), tet(B), sul1, sul2, sul3, aph(3”)-Ib (strA), aph(6)-Id (strB), aadA1, aadA2, and aadA5. ESBL analysis revealed two predominant phenotypes: AmpC (51%) and ESBL (46%) where blaCMY−2 and blaCTX−M(blaCTX−M−1, blaCTX−M−15, and blaCTX−M−55) were the genes responsible for these phenotypes, respectively. During this study, 85% of farms had at least one ESBL/AmpC producing E. coli. Isolates from calves were more frequently resistant than those from cows or manure pits. Although prevalence of AMR was low for critically important antimicrobials, there was a high prevalence of ESBL/AmpC-producing E. coli on Quebec dairy farms, particularly in calves. Those data will help determine a baseline for AMR to evaluate impact of initiatives aimed at reducing AMR.

scholarly journals Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

2013 ◽  
Vol 79 (9) ◽  
pp. 3027-3032 ◽  
Author(s):  
A. Schmid ◽  
S. Hörmansdorfer ◽  
U. Messelhäusser ◽  
A. Käsbohrer ◽  
C. Sauter-Louis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Dairy Cows ◽  
Fecal Samples ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Study Population ◽  
Cattle Farms ◽  
Group 1

ABSTRACTExtended-spectrum β-lactamase (ESBL)-producingEscherichia colistrains are believed to be widely distributed among humans and animals; however, to date, there are only few studies that support this assumption on a regional or countrywide scale. Therefore, a study was designed to assess the prevalence of ESBL-producingE. coliin dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs, and dust samples were analyzed for ESBL-producingE. coliusing selective media. PCR was performed to screen for CTX-M andampCresistance genes. A total of 598 samples yielded 196 (32.8%) that contained ESBL-producingE. coli, originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL-producingE. colipositive than samples from beef cattle farms (fecal samples,P< 0.001; boot swabs,P= 0.014; and dust samples,P= 0.041). A total of 183 isolates (93.4%) of 196 ESBL-producingE. coli-positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty-six additional isolates containedampCgenes, and 5 of the 46 isolates expressed ablaCMY-2gene. The study shows that ESBL-producingE. colistrains are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence ofblaCMY-2in cattle in Germany.

Factors Associated with Fecal Shedding of Verotoxin-Producing Escherichia coli O157 on Dairy Farms

1999 ◽  
Vol 62 (4) ◽  
pp. 307-312 ◽  
Author(s):  
L. GARBER ◽  
S. WELLS ◽  
L. SCHROEDER-TUCKER ◽  
K. FERRIS
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Odds Ratio ◽  
Dairy Farms ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Factors Associated

Fecal samples were collected from 4,361 dairy cows on 91 dairy operations between 26 February and 8 July 1996. Fecal samples were cultured for Escherichia coli O157, and positive isolates were probed for verotoxin-producing genes. A total of 52 (1.2%) fecal samples on 22 (24.2%) operations were positive for verotoxin-producing E. coli O157. Herds in which samples were collected on or after 1 May 1996 were significantly more likely to test positive than herds sampled before that date (odds ratio = 7.7). Herds maintained on farms on which alleyways were flushed with water to remove manure were 8.0 times more likely to have samples test positive for verotoxin-producing E. coli O157 than were herds maintained on farms cleaned by use of other methods of manure removal.

scholarly journals Carriage of Escherichia coli Producing CTX-M-Type Extended-Spectrum β-Lactamase in Healthy Vietnamese Individuals

2015 ◽  
Vol 59 (10) ◽  
pp. 6611-6614 ◽  
Author(s):  
Thi Mai Huong Bui ◽  
Itaru Hirai ◽  
Shuhei Ueda ◽  
Thi Kim Ngan Bui ◽  
Kouta Hamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTHealthy carriage of CTX-M-type extended-spectrum β-lactamase (ESBL)-producingEscherichia coliwas examined by thrice collecting fecal samples from the same 199 healthy Vietnamese subjects every 6 months. Using pulsed-field gel electrophoresis (PFGE), identical PFGE patterns throughout the three samplings were not observed, although prevalence ofE. coliin the subjects was around 50% in the three samplings. Our results suggested a short carriage period of the CTX-M-type ESBL-producingE. coliin healthy Vietnamese subjects.

Sign in / Sign up

Export Citation Format

Share Document