scholarly journals Immunomodulatory, behavioral, and nutritional response of tryptophan application on poultry

2021 ◽  
pp. 2244-2250
Author(s):  
Nguyen Thuy Linh ◽  
Budi Guntoro ◽  
Nguyen Hoang Qui

Tryptophan is an essential amino acid for all animals that was discovered through casein hydrolysis. The use of tryptophan as feed additives has been attracting the attention of many nutritionists because it cannot be synthesized enough in an animal's body. Tryptophan or precursor to the vitamin niacin in the diet is important, and its supplementation for poultry is determined to improve the amino acid balance and promote the poultry's growth performance through enhancing appetite, feed efficiency, and protein synthesis. Moreover, poultry in different growth phases, breeding, and conditions require various amounts of tryptophan. In addition, supplemented tryptophan also improves the immune response or the immunomodulatory activity of poultry to various diseases through the kynurenine pathway, especially diseases in the bursa. Furthermore, tryptophan also has a strong relationship with lysine (the ideal tryptophan/lysine ratio) in improving growth performance. However, tryptophan deficiency could affect the behavioral responses (e.g. pecking behavior and poultry stress) because tryptophan serves as a precursor for the neurotransmitter serotonin and the pineal hormone melatonin in the diet. This paper tried to summarize all information about applying tryptophan in the diets and illustrate the roles of tryptophan in the poultry industry.

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Sangwoo Park ◽  
Jung Wook Lee ◽  
Kevin Jerez Bogota ◽  
David Francis ◽  
Jolie Caroline González-Vega ◽  
...  

Abstract This study was conducted to investigate the effects of a direct-fed microbial (DFM) product (Bacillus subtilis strain DSM 32540) in weaned pigs challenged with K88 strain of Escherichia coli on growth performance and indicators of gut health. A total of 21 weaned pigs [initial body weight (BW) = 8.19 kg] were housed individually in pens and fed three diets (seven replicates per diet) for 21 d in a completely randomized design. The three diets were a corn-soybean meal-based basal diet without feed additives, a basal diet with 0.25% antibiotics (neo-Oxy 10-10; neomycin + oxytetracycline), or a basal diet with 0.05% DFM. All pigs were orally challenged with a subclinical dose (6.7 × 108 CFU/mL) of K88 strain of E. coli on day 3 of the study (3 d after weaning). Feed intake and BW data were collected on days 0, 3, 7, 14, and 21. Fecal scores were recorded daily. On day 21, pigs were sacrificed to determine various indicators of gut health. Supplementation of the basal diet with antibiotics or DFM did not affect the overall (days 0–21) growth performance of pigs. However, antibiotics or DFM supplementation increased (P = 0.010) gain:feed (G:F) of pigs during the post-E. coli challenge period (days 3–21) by 23% and 24%, respectively. The G:F for the DFM-supplemented diet did not differ from that for the antibiotics-supplemented diet. The frequency of diarrhea for pigs fed a diet with antibiotics or DFM tended to be lower (P = 0.071) than that of pigs fed the basal diet. The jejunal villous height (VH) and the VH to crypt depth ratio (VH:CD) were increased (P < 0.001) by 33% and 35%, respectively, due to the inclusion of antibiotics in the basal diet and by 43% and 41%, respectively due to the inclusion of DFM in the basal diet. The VH and VH:CD for the DFM-supplemented diet were greater (P < 0.05) than those for the antibiotics-supplemented diet. Ileal VH was increased (P < 0.05) by 46% due to the inclusion of DFM in the basal diet. The empty weight of small intestine, cecum, or colon relative to live BW was unaffected by dietary antibiotics or DFM supplementation. In conclusion, the addition of DFM to the basal diet improved the feed efficiency of E. coli-challenged weaned pigs to a value similar to that of the antibiotics-supplemented diet and increased jejunal VH and VH:CD ratio to values greater than those for the antibiotics-supplemented diet. Thus, under E. coli challenge, the test DFM product may replace the use of antibiotics as a growth promoter in diets for weaned pigs to improve feed efficiency and gut integrity.


2020 ◽  
Vol 28 (2) ◽  
pp. 63
Author(s):  
Pablo Jesús Marín-García ◽  
María del Carmén López-Luján ◽  
Luís Ródenas ◽  
Eugenio Melchor Martínez-Paredes ◽  
Enrique Blas ◽  
...  

<p>In recent decades, recommendations on dietary protein content have been considerably reduced, while fibre content has been increased. Under these conditions, an adequate dietary amino acid balance could be crucial to optimise feed efficiency. Plasma urea nitrogen (PUN) level could be a good indicator of an amino acid imbalance and its potential has already been studied in other species, but not yet in rabbits. The main objective of the present work was to detect the possible interest of PUN in pinpointing amino acid deficiencies in rabbits. Two experimental diets were formulated from the same basal mixture, following all the recommendations for growing rabbits, except lysine, whose content was variable, following current guidelines in diet P8.1 or lower from those in P4.4 (with 8.1 and 4.4 g/kg dry matter of lysine and with 757 and 411 mg of lysine per MJ of digestible energy). Three different trials were designed: one where the animals were fed &lt;em&gt;ad libitum&lt;/em&gt; (AL) and two others in which fasting periods of 10 h were included; one where feeding was restored at 08:00 h (Fast8h) and the other at 18:00 h (Fast18h). A total of 72 three-way crossbred growing rabbits (24 animals for each trial in a split-plot trial) up to a total of 12 recordings were used. Blood samples were taken every 4 h in AL trial and every hour after refeeding up to a total of six controls, in trials Fast8h and<br />Fast18h. The differences between balanced and unbalanced diets in lysine were highest (&lt;em&gt;P&lt;/em&gt;&lt;0.001) between 04:00 h and 12:00 h in animals fed ad libitum, and at 3 h after refeeding (21:00 h) in Fast18h. These results suggest that PUN could be an adequate indicator to detect deficiencies in amino acids in growing rabbit<br />diets.</p>


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 12-13
Author(s):  
So-Young Kim

Abstract Global Amino acids market is estimated as $15billion in 2020 and 60% of those volume is used for feed additives. Amino acids are converted to protein efficiently and have a benefit of lowering feeding cost and reducing the nitrogen content of the waste output compared to intact protein diet. Most amino acids are produced using microbial fermentation. Traditionally, amino acids used for feed additives are Lysine, threonine, tryptophan and methionine. Among them methionine was produced only by chemical process, but now methionine can also be produced by microbial fermentation, which can enlarge the choice of methionine from DL-form to L-form. Use of L-form methionine as feed additives reveals new nutritional value of L-methionine compared to DL-methionine. Recently, valine, arginine, isoleucine, and histidine are also included in feeds as functional nutrients thanks to their commercial availability and lower price. Technical progress in microbiology and bioengineering has been made more diverse amino acid to be used as feed additives. Then what would be the next amino acid as feed additivities based on microbial point of view? Leucine, glutamine, tyrosine and phenylalanine could be next possible amino acids based on metabolic pathways of microorganism. These amino acids share the pathways with former feed amino acids such as valine or tryptophan, which means that these can be produced with way that is more economical. In addition, these amino acids could be produced as mixed forms like glutamic acid/glutamine mixture, Tryptophan/tyrosine/phenylalanine mixture, or BCAA forms with cheaper price. Dried fermentation product such as lysine sulfate made by drying of microbial culture broth, could be one of the option for the combination of several new amino acids. Crude protein reduction in feed is one of the big nutritional trend to reach the ideal protein diet and environmental clean situation. Considering the use of new synthetic amino acids in feeds would be a potential way to realize the ideal protein diet.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 182-182
Author(s):  
Monika Hejna ◽  
Matteo Dell’Anno ◽  
Valentina Caprarulo ◽  
Stefania Sotira ◽  
Luciana Rossi

Abstract Weaned piglets often suffer from the enteric disorders and post-weaning diarrhoea. Natural extracts, due to its functional properties, such as tannins from chestnut and quebracho are often considered as feed additives against diarrhoea after the antibiotics ban (Reg. UE 1831/2003). The aim of this study was to evaluate the dietary effects of tannins on growth performance and blood parameters of weaned piglets. A total of 120 piglets (Large White x Landrace) weaned at d 28±2 were randomly allotted to one of two treatments (control vs. tannin) with 6 pens per treatment and 10 pigs per pen. The tannin diet was supplemented with 1.25% of chestnut and quebracho tannins (Silvateam, Italy). The experiment lasted 40 days. Individual body weight (BW) was recorded at d 0, 14, 28 and 40. Feed intake was measured weekly to calculate feed efficiency from d 0 to 14, d 14 to 28, and d 28 to 40. Blood samples were collected on d 40 from a subset of animals (4 pigs/pen). Data were analyzed using PROC GLIMMIX of SAS 9.4 (SAS Inst. Inc., Cary, NC). Supplementation of tannins did not affect BW and feed intake throughout the experiment. However, addition of tannins showed tendency to increase the feed efficiency on d 14 to d 28, compared with control (P = 0.54; 60.4 vs. 52.3, respectively). Dietary addition of tannins significantly increase the serum concentration of albumin, and decrease of globulin, urea and creatinine compared with control (P &lt; 0.05; 23.05 vs. 19.31 g/L, 30.13 vs. 33.58 g/L; 0.98 vs. 2.18 mmol/L; 53.92 vs. 78.92 μmol/L, respectively). In conclusion, tannin supplementation impacted the blood parameters that were related to protein utilization, although this benefit was not reflected in growth performance of weaned pigs.


2019 ◽  
Vol 19 (2) ◽  
pp. 271 ◽  
Author(s):  
Siti Aslamyah ◽  
Zainuddin Zainuddin ◽  
Badraeni Badraeni

The quality of feed can be improved by fermenting feedstuffs and supplementing with feed additives. This study aims to determine the effect of supplementation of Lumbricus sp. extract in fermented feed on growth performance, body chemical composition, and milkfish hepatosomatic index. Milkfish with an initial weight of 17.80 ± 0.20 g head-1, rearing with a density of 20 fish per hapa net measuring 1 m3 by 24 pieces. Hapa net is installed in a pond with a water level of ± 60 cm. Research was design in completely randomized design with two factors. The first-factor was supplementation method, namely Lumbricus sp. which sprayed on feed and mixed with feed ingredients. The second factor was the dose of Lumbricus sp. extract, namely 0, 100, 200 and 300 mL kg-1 of feedstuffs. The fish was reared for 50 days and fed with 5% of fish biomass per day with feeding frequency of 3 times i.e., morning, afternoon and evening. The results showed that the supplementation Lumbricus sp. extract has no significant effect on all parameters (P>0,05).  However, the level dose of Lumbricus sp. extract supplementation in feed has a significantly effect (P<0.05) on absolute growth, relative growth, feed efficiency, and hepatosomatic index, but no significant effect (P>0,05) on survival and chemical composition of milkfish body. The best best of absolute growth (16.94±4.0 g), relative growth (48.71±5.77%), feed efficiency (40.74±10.3 %), and hepatosomatic index (1.5 ± 0.17) were found in the experiment of supplementation Lumbricus sp. extract mixed with feedstuffs at a dose of 300 mL kg1. The survival rate of milkfish was ranging from 68.33±29.3 to 91.,33±7.64%, while the body's chemical composition including protein levels, fat, ash, crude fiber, NFE / Nitrogen Free Extract, liver glycogen and muscle glycogen were 69.45±1.23 – 71.45±0.97%, 14.86±0.46 – 17.24±0.76%, 9.28±0.12 – 11.12±0.46%, 1.54±0.09 – 1.66 ± 0.13%, 1.33± 0.42 – 2.71±0.21%, 7,11±0,08 – 7,40±0,17mg g-1 and 6,13±0,44 – 6,45±0,40 mg g-1, respectively.


2018 ◽  
Vol 27 (2) ◽  
pp. 337-347 ◽  
Author(s):  
Alberto J. P. Nunes ◽  
Hassan Sabry-Neto ◽  
Francisco Hélio Pires da Silva ◽  
Adhemar Rodrigues de Oliveira-Neto ◽  
Karthik Masagounder

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