scholarly journals The effect of supplementation of Lumbricus sp. extract in fermented foods for growth performance, body chemical composition, and hepatosomatic index of milkfish, Chanos chanos Forsskal, 1775

2019 ◽  
Vol 19 (2) ◽  
pp. 271 ◽  
Author(s):  
Siti Aslamyah ◽  
Zainuddin Zainuddin ◽  
Badraeni Badraeni

The quality of feed can be improved by fermenting feedstuffs and supplementing with feed additives. This study aims to determine the effect of supplementation of Lumbricus sp. extract in fermented feed on growth performance, body chemical composition, and milkfish hepatosomatic index. Milkfish with an initial weight of 17.80 ± 0.20 g head-1, rearing with a density of 20 fish per hapa net measuring 1 m3 by 24 pieces. Hapa net is installed in a pond with a water level of ± 60 cm. Research was design in completely randomized design with two factors. The first-factor was supplementation method, namely Lumbricus sp. which sprayed on feed and mixed with feed ingredients. The second factor was the dose of Lumbricus sp. extract, namely 0, 100, 200 and 300 mL kg-1 of feedstuffs. The fish was reared for 50 days and fed with 5% of fish biomass per day with feeding frequency of 3 times i.e., morning, afternoon and evening. The results showed that the supplementation Lumbricus sp. extract has no significant effect on all parameters (P>0,05).  However, the level dose of Lumbricus sp. extract supplementation in feed has a significantly effect (P<0.05) on absolute growth, relative growth, feed efficiency, and hepatosomatic index, but no significant effect (P>0,05) on survival and chemical composition of milkfish body. The best best of absolute growth (16.94±4.0 g), relative growth (48.71±5.77%), feed efficiency (40.74±10.3 %), and hepatosomatic index (1.5 ± 0.17) were found in the experiment of supplementation Lumbricus sp. extract mixed with feedstuffs at a dose of 300 mL kg1. The survival rate of milkfish was ranging from 68.33±29.3 to 91.,33±7.64%, while the body's chemical composition including protein levels, fat, ash, crude fiber, NFE / Nitrogen Free Extract, liver glycogen and muscle glycogen were 69.45±1.23 – 71.45±0.97%, 14.86±0.46 – 17.24±0.76%, 9.28±0.12 – 11.12±0.46%, 1.54±0.09 – 1.66 ± 0.13%, 1.33± 0.42 – 2.71±0.21%, 7,11±0,08 – 7,40±0,17mg g-1 and 6,13±0,44 – 6,45±0,40 mg g-1, respectively.

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Sangwoo Park ◽  
Jung Wook Lee ◽  
Kevin Jerez Bogota ◽  
David Francis ◽  
Jolie Caroline González-Vega ◽  
...  

Abstract This study was conducted to investigate the effects of a direct-fed microbial (DFM) product (Bacillus subtilis strain DSM 32540) in weaned pigs challenged with K88 strain of Escherichia coli on growth performance and indicators of gut health. A total of 21 weaned pigs [initial body weight (BW) = 8.19 kg] were housed individually in pens and fed three diets (seven replicates per diet) for 21 d in a completely randomized design. The three diets were a corn-soybean meal-based basal diet without feed additives, a basal diet with 0.25% antibiotics (neo-Oxy 10-10; neomycin + oxytetracycline), or a basal diet with 0.05% DFM. All pigs were orally challenged with a subclinical dose (6.7 × 108 CFU/mL) of K88 strain of E. coli on day 3 of the study (3 d after weaning). Feed intake and BW data were collected on days 0, 3, 7, 14, and 21. Fecal scores were recorded daily. On day 21, pigs were sacrificed to determine various indicators of gut health. Supplementation of the basal diet with antibiotics or DFM did not affect the overall (days 0–21) growth performance of pigs. However, antibiotics or DFM supplementation increased (P = 0.010) gain:feed (G:F) of pigs during the post-E. coli challenge period (days 3–21) by 23% and 24%, respectively. The G:F for the DFM-supplemented diet did not differ from that for the antibiotics-supplemented diet. The frequency of diarrhea for pigs fed a diet with antibiotics or DFM tended to be lower (P = 0.071) than that of pigs fed the basal diet. The jejunal villous height (VH) and the VH to crypt depth ratio (VH:CD) were increased (P &lt; 0.001) by 33% and 35%, respectively, due to the inclusion of antibiotics in the basal diet and by 43% and 41%, respectively due to the inclusion of DFM in the basal diet. The VH and VH:CD for the DFM-supplemented diet were greater (P &lt; 0.05) than those for the antibiotics-supplemented diet. Ileal VH was increased (P &lt; 0.05) by 46% due to the inclusion of DFM in the basal diet. The empty weight of small intestine, cecum, or colon relative to live BW was unaffected by dietary antibiotics or DFM supplementation. In conclusion, the addition of DFM to the basal diet improved the feed efficiency of E. coli-challenged weaned pigs to a value similar to that of the antibiotics-supplemented diet and increased jejunal VH and VH:CD ratio to values greater than those for the antibiotics-supplemented diet. Thus, under E. coli challenge, the test DFM product may replace the use of antibiotics as a growth promoter in diets for weaned pigs to improve feed efficiency and gut integrity.


2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Andris Pasiali ◽  
Cyska Lumenta ◽  
Diane J. Kusen

The aim of the study was to determine the effect of different compositions of kedondong laut leaf flour in feed formulations on absolute growth, daily growth, relative growth, feed efficiency, food conversion ratio. The test animals used were Nile tilapia with a size of 3-5cm in length and weigh between 1.3 - 2 g.  Fish were stocked in nets measuring 50x50x50 cm3 with a density of 10 fish each.  Fish were obtained from the Tatelu Aquaculture Fisheries Center, Dimembe District, North Minasaha Regency. The study was conducted by an experimental method using a complete randomized design (CRD).  The treatments consisted of A without addition of kedondong laut leaves flour, Treatment B with addition of 10% kedondong laut leaf flour, Treatment C with addition of 20% kedondong laut leaf flour, Treatment D with addition of 30% kedondong laut leaf flour. The results of the experiment showed that treatment B was the best where absolute growth (23%), relative growth (287.3%), daily growth (3.93%), feeding efficiency (95.83 %) and food conversion ratio (1,044). Water quality during the experiment was in a reasonable range where the water temperature during experiment was 26oC, pH 7-8


2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Herol A. Tulung ◽  
Cyska Lumenta ◽  
Edwin L.A. Ngangi

This study aimed to determine the effect of papaya flour at different doses on absolute  growth, daily growth, relative growth, feed efficiency, and food conversion. The treatments consisted of 0,  2.5%, 7.5%, 10% of papaya flour respectively, each with three replcations. The weight of fish were  about 18-23.58 g.  The fish were placed in aquaria with a density of  10 individual per aquarium. The experiment design is a complete randomized design. The results of analysis show that the treatments had no significant effect on growth, daily growth, relative growth, feed  efficient, and food conversion ratio. However, treatment C (7.5% papaya flour) gave the best on growth of fish, feed efficiency and food conversion ratio.


Author(s):  
Siti Aslamyah ◽  
Muh. Yusri Karim ◽  
Badraeni Badraeni

Mixed microorganisms consist of bacteria, fungi, yeasts, and molds that produce important enzymes to ferment raw materials, thereby increasing the nutrient content of milkfish feed. This study aims to examine various doses of mix. microorganisms fermented raw materials on growth performance, chemical composition of the body, and hepatosomatic index of milkfish. Milkfish weighing 28.40 ± 0.11 g is kept in hapa of 1 m3 and placed on brackish water ponds. The test feed contains Sargassum sp. and formulated from raw materials fermented with mix. microorganism (control, 10, 15 and 20 mL / 100 g). The experimental results show the relative growth rate (%) and the growth of biomass (g) of milkfish fed with fermented feedstock with a dose of 10 mL / 100 g (122.77 ± 3.00%, and 522.05 ± 12.19 g ), the real feed is higher than the other dose. The fat content, crude fiber, and energy of test fish fed with fermented feedstock with 10, 15, and 20 mL / 100 g doses were significantly lower, while the hepatosomatic index was significantly higher than the control. (91.11-97,78%), protein content and NFE or Nitrogen Free Extracts (66.81 ± 0.41 72.33 ± 0.28% and 0.88 ± 0.38- 2.24 ± 0.92%), hepatic and muscle glycogen (7.16 ± 0.25-7.3 ± 0.31 mg / g and 6.16 ± 0.16, 6.51 ± 0.35 mg / g) were the same in all treatments. Thus, a dose of 10 mL / 100 g feed ingredients is the best dose in fermenting feed ingredients.Keywords: fermentation,  milkfish,  mix. microorganism, artificial feed, Sargassum sp.


Author(s):  
M. Yu. Sychov ◽  
H. I. Pryumak

<p>The authors have investigated the effect of different levels guanidinoacetic acid in the diets of quails on their productivity and carcass quality. Experimental studies were carried out in the conditions of the problematic research laboratory of feed additives in the National University of Life and Environmental Sciences of Ukraine. Material for scientific and economic experience was quail of Pharaoh species of meat direction of productivity. The experiment was carried out by method of groups-analogues. Compound feed were fed in dry crumbled form. Young animals were fed in a group. The daily amount of compound feed was divided in two parts – morning and evening rations. The effect of using different levels of Guanidinoacetic Acid (GAA) in compound feed on productivity and carcass quality of young quails was studied. We have found that compound feed with the level of GАА 0.06% contributes to live body weight increase by 3.9 g and muscles of the pelvic limbs yield by 26.2%. At the same time, use of diets with a level of 0.12% of guanidine acetate increases live weight by 13.5 g, the absolute growth of 6.5 g, average daily growth by 0.9 g, a relative growth of 2%, the mass of carcass, semi-eviscerated carcass, and eviscerated carcass of 4.8, 4.9 and 4.3% correspondingly, slightly increases the mass of pectoral muscles by 9.1%, significantly increases the yield of muscles of the pelvic limbs by 36.4%, and reduces feed conversion by 5%. Poultry which was fed with 0.18% of GАА exceeded control of body weight by 12.2 g, the absolute growth by 4.4 g, average daily growth by 0.6 g, the relative growth by 1.1%, yield of muscles of the pelvic limbs by 36.4% and  had a lower feed conversion rate by 9.1%. During the study period the safety of livestock which was fed with GААvwas high and ranged from 90 to 96%.  It was established the perspective of further research, which is to examine the impact of Guanidinoacetic Acid on the egg production of egg-laying quails, morphological and chemical composition of eggs and  their hatching  quality.</p>


2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Faisal Mujiono ◽  
Julius Sampekalo ◽  
Cyska Lumenta

The objectives of research were to evaluate the effect bakasang-supplemented commercial diet on nile tilapia growth and to find the optimum dosage of bakasang to be supplemented in feed. The research was conducted at Laboratory of Nutrition and Fish Feed Technology.  Fish weighing 0,9-1,7 g were palced in 15 aquaria at a density of 10 individuals/aquarium. Experimental design was complete randomized design with five treatments namely A (0% bakasang), B (2% bakasang), C (3% bakasang), D (4% bakasang), E (5% bakasang), each with three replications. Fish were fed three times a day as much as 5%/body weight. Growth of fish was observed every week. Research results showed that the lowest absolute growth, relative growth and feed efficiency  were observed in treated with 0% bakasang (êG=20.44 g; GR=148.08%; dan NEP=58.06%) while the highest was obtained in fish treated with 4% bakasang (êG=35,55 g; GR=262.17%; NEP=80.53%).  Analysis of variance displayed the use of bakasang significantly affect absolute growth, relative growth. It was concluded that feed supplemented with 4% bakasang gave better absolute growth, relative growth and feed efficiency as compared to other feed.   Keywords: growth, “bakasang”, absolute growth, relative growth, feed efficiency,  nile tilapia


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Rian M. Nanariain ◽  
Cyska Lumenta ◽  
Henneke Pangkey

The objective of this research was to evaluate the effect of supplementation of banana skin (Musa paradisiaca) in feed formulation on absolute growth, relative growth, daily growth and feed efficiency of nile tilapia.  The research was conducted at the Laboratory of Aquaculture Technology, Faculty of Fisheries and Marine Science, Sam Ratulangi University. The research used Complete Randomized Design with five treatments inluded A: without supplementation banana skin powder, B: 40%, C: 30%, D: 20% and E: 10% of banana skin powder. Each treatment  had three replications.  The result showed the best response was observed in treatment D  with absolute growth o43.11 g, relative growth 88.9%, daily growth 10.17%,  and feed efficiency 3.20%. Keywords:  feed formulation, banana skin, nile tilapia, absolute growth, relative growth,   daily growth,  feed efficiency


Author(s):  
O. A. Osinowo ◽  
C. C. Ogbonna ◽  
A. M. Omoare

Aims: Growth performance and gut morphological response of broiler chickens fed moist fermented diets with the inclusion of probiotics or/and organic acids were evaluated in a 56 day feeding trial. Place and Duration of Study: Poultry unit of the Department of Agricultural Education, Federal College of Education, Abeokuta, Ogun State, Nigeria between February 2018 and April 2018 Methodology: Three hundred one-day old unsexed Abor Acre broilers were used for this study. They were randomly allotted to 5 dietary treatments with 6 replicates of 10 birds each. The experimental treatments were: Diet 1: Dry Unfermented Feed (DUF) - Probiotics (Pr) – Organic acids (Or), Diet 2: Moist Fermented Feed (MFF) – Pr – Or, Diet 3: MFF + Pr, Diet 4: MFF + Or, Diet 5: MFF + Pr + Or. The experiment was carried out using a completely randomized design. Results: The feed conversion ratio of broiler starters fed diet 5 (1.96) was significantly (P<.0001) better relative to those of birds fed other diets (2.27, 2.21, 2.14 and 2.13 respectively), while birds in treatment 1 (2.27) had significantly (P<.0001) poor feed conversion ratio. There were no significant (P>.05) differences in the feed conversion ratio of broiler finishers fed MFF with or without feed additives (2.68, 2.64, 2.55 and 2.55). However, the value of feed conversion ratio of birds fed DUF (3.04) was significantly (P=.05) the highest. The duodenal villus height to crypt depth ratio of broiler starters fed MFF+ Pr + Or (7.11) was significantly (P=.05) higher than those of birds fed other diets (2.70, 3.35, 3.98, 4.73 respectively). Conclusion: Although, feed fermentation enhanced gut morphological parameters which correlates improved growth performance of broiler chickens. The inclusion of probiotics (1g/kg) and organic acids (1g/kg) in the fermentation process further improved the growth indicators of broiler chickens used in this study.


Author(s):  
Ebru Yılmaz ◽  
Deniz Çoban ◽  
Birsen Kırım ◽  
Mehmet Güler

This study was performed to determine the effects of the extracts of two alternative antimicrobial agents that do not harm the ecosystem (rosemary, Rosmarinus officinalis and aloe vera, Aloe barbadensis) when added to the feed of tilapia (Oreochromis niloticus) on the growth performance, proximate composition, and biometric characteristics. Rosemary extract was added to the experimental diet in ratios of 0.1%, 0.25%, and 0.5% while aloe vera extract was added to the experimental diet in ratios of 0.5%, 1%, and 2.5%. After 90 days of the feeding trial, no change was seen in the weight increase, specific growth rate, feed conversion ratio, condition factor, hepatosomatic index, or viscerosomatic index of the tilapia. The fact that proximate analyses did not differ between groups showed that the plant extracts did not adversely affect the health status of tilapia in the ratios used in this study. As a result, the addition of 0.1%, 0.25%, and 0.5% rosemary extract and the addition of 0.5%, 1%, and 2.5% aloe vera extract to tilapia feeds did not cause changes in the growth performance, biometric indexes, or chemical composition findings of the fish meat.


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