scholarly journals Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara

2021 ◽  
Vol 24 (2) ◽  
pp. 66
Author(s):  
Indriati Ramadhani ◽  
Hasnadiazahra Rohadi ◽  
Yeni Yuliani ◽  
Muhammad Ilyas
Keyword(s):  
Endophytic Fungi ◽  
Host Plants ◽  
Moringa Oleifera ◽  
Fungal Endophytes ◽  
Slight Modification ◽  
Morphological Observation ◽  
Surface Sterilization ◽  
Fungal Strains ◽  
Lombok Island ◽  
Moringa Oleifera Lam

An assemblage of endophytic fungi was isolated from Moringa oleifera Lam. collected from Lombok island, West Nusa Tenggara Province, Indonesia. Fungal endophytes were isolated using surface sterilization methods with slight modification. Forty-six selected endophytic fungal strains were isolated from the leaves, petioles, and stems of M. oleifera. The fungal strains identification through morphological observation and ITS rDNA-based molecular analysis showed that fungal endophytes were associated with host plants belonging to the taxa Alternaria, Cladosporium, Colletotrichum (Glomerella), Corynespora, Curvularia (Cochliobolus), Fusarium, Mucor, Ochrocladosporium, Phomopsis (Diaporthe), and Trametes. In this study, endophytic Phomopsis dominating the obtained strains, whereas 26% (12/46) strains were isolated from host plant samples.

scholarly journals Diversity of Foliar Endophytic Fungi in Artocarpus heterophyllus Lam. and Citrus reticulata Blanco of Tripura

2021 ◽  
Vol 11 (2) ◽  
pp. 251-258
Author(s):  
Sanchita Bhattacharya ◽  
◽  
Sanjit Debnath ◽  
Ajay Saha ◽  
◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Host Plants ◽  
Fungal Endophytes ◽  
Citrus Reticulata ◽  
Artocarpus Heterophyllus ◽  
Fungal Strains ◽  
Citrus Reticulata Blanco

Present investigation dealt with the isolation and diversity of foliar fungal endophytes from two fruit yielding plants of Tripura state. Seasonal distributions of endophytes in respect to fruiting and non-fruiting season in two host plants were also studied. Twenty one fungal strains along with one nonsporulating hyaline form were isolated as foliar endophytes from the two host plants. In both fruiting and non-fruiting seasons, from Citrus reticulata seven foliar endophytic fungi were isolated. Whereas from Artocarpus heterophyllus in both the season eleven fungal endophytes with one nonsporulating hyaline form were isolated. The relative frequencies of isolated fungi from both A. heterophyllus and C. reticulata in fruiting and non-fruiting season significantly differed among the sampling sites, whereas relative frequencies individual endophytic fungus showed no significant differences among various sampling sites.

scholarly journals Isolation and Mophological Identification of Endophytic Fungi from Moringa Plant (Moringa oleifera Lam.)

2019 ◽  
Vol 1 (2) ◽  
pp. 123
Author(s):  
Hasnadhiazahra Rohadi ◽  
Nuraeni Ekowati ◽  
Moh Ilyas
Keyword(s):  
Endophytic Fungi ◽  
Pure Culture ◽  
Moringa Oleifera ◽  
Fungal Endophytes ◽  
Morphological Characteristics ◽  
Plant Tissues ◽  
Morphological Identification ◽  
Mycelia Sterilia ◽  
Endophytic Microorganism ◽  
Moringa Oleifera Lam

Moringa oleifera Lam. is a small, perennial tree which belongs to moringaceae family. This plant is widely known for their nutritional and medicinal purposes. They can associate with microorganisms showing beneficial symbiosis called as endophytic microorganism. Endophytic fungi is microorganism capable of living within the plant tissues without harming their host.  This study was aimed for obtaining pure culture of fungal endophytes from M. oleifera and then identifying them based on morphological identification. The morphological identification was done by observing both macro- and micro- morphological characteristics of the endophytic fungi. Twenty endophytic fungi were isolated from lamina, petiole and stem of M. oleifera. The genus obtained were Cladosporium, Colletotrichum, Fusarium, Phomopsis, Phyllosticta, white mycelia sterilia and black mycelia sterilia (Dematiaceae). Keywords: endophytic fungi; identification; isolation; morphology; Moringa oleifera Lam.

scholarly journals ISOLASI CENDAWAN ENDOFIT DARI AKAR WANGI (Vetiveria zizanioides L.) DAN POTENSINYA SEBAGAI ANTIMIKROBA

1930 ◽  
Vol 8 (01) ◽  
pp. 115-121
Author(s):  
Israwati Harahap ◽  
Elsie
Keyword(s):  
Escherichia Coli ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Vetiveria Zizanioides ◽  
Surface Sterilization ◽  
Anti Oxidant ◽  
In Vitro Inhibition ◽  
Fungal Extracts ◽  
Antimicrobial Potency

Endophytic fungi are microorganisms living in healthy tissue of their host plants without causing disease. Endophytic fungi live in every plant, including Vetiveria zizanioides L. medicinal plant intracellulary and/or intercellulary. This plant was screened pharmacologically for antibacterial, antifungal, anticataleptic, analgesic and anti-inflammatory, rheumatism, anti oxidant and anti arthritic activity. This research aimed to isolation endophytic fungi from V. zizanioides L. and to screening their antimicrobial potency against microflora Escherichia coli, Staphylococcus aureus, and Candida albicans. Endophytic fungi isolation from V. zizanioides L. was done by surface sterilization method. A total of 33 isolates were recovered from 88 leaves and 88 root segments. Based on antimicrobial activitytest, most of the fungal extracts showed in vitro inhibition of microbes growth.

Chemical and Functional Diversity of Natural Products from Plant Associated Endophytic Fungi

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Vijay C. Verma ◽  
Ravindra N. Kharwar ◽  
Gary A. Strobel
Keyword(s):  
Natural Products ◽  
Biological Activity ◽  
Functional Diversity ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Fungal Endophytes ◽  
Sources Identification ◽  
Naturally Occurring

This review describes examples of naturally occurring bioactive compounds obtained from fungal endophytes from various host plants. The main topics addressed are sources, identification, biological activity, biosynthesis, and ecological and chemosystematic significance of those bioactive compounds whose sources were well defined.

scholarly journals Diverse Bacteria Inhabit Living Hyphae of Phylogenetically Diverse Fungal Endophytes

2010 ◽  
Vol 76 (12) ◽  
pp. 4063-4075 ◽  
Author(s):  
Michele T. Hoffman ◽  
A. Elizabeth Arnold
Keyword(s):  
Endophytic Fungi ◽  
Tree Species ◽  
Host Plants ◽  
Abiotic Factors ◽  
Fungal Endophytes ◽  
Molecular Techniques ◽  
Biogeographic Provinces ◽  
Viable Bacteria ◽  
Bacterial Endosymbionts ◽  
First Time

ABSTRACT Both the establishment and outcomes of plant-fungus symbioses can be influenced by abiotic factors, the interplay of fungal and plant genotypes, and additional microbes associated with fungal mycelia. Recently bacterial endosymbionts were documented in soilborne Glomeromycota and Mucoromycotina and in at least one species each of mycorrhizal Basidiomycota and Ascomycota. Here we show for the first time that phylogenetically diverse endohyphal bacteria occur in living hyphae of diverse foliar endophytes, including representatives of four classes of Ascomycota. We examined 414 isolates of endophytic fungi, isolated from photosynthetic tissues of six species of cupressaceous trees in five biogeographic provinces, for endohyphal bacteria using microscopy and molecular techniques. Viable bacteria were observed within living hyphae of endophytic Pezizomycetes, Dothideomycetes, Eurotiomycetes, and Sordariomycetes from all tree species and biotic regions surveyed. A focus on 29 fungus/bacterium associations revealed that bacterial and fungal phylogenies were incongruent with each other and with taxonomic relationships of host plants. Overall, eight families and 15 distinct genotypes of endohyphal bacteria were recovered; most were members of the Proteobacteria, but a small number of Bacillaceae also were found, including one that appears to occur as an endophyte of plants. Frequent loss of bacteria following subculturing suggests a facultative association. Our study recovered distinct lineages of endohyphal bacteria relative to previous studies, is the first to document their occurrence in foliar endophytes representing four of the most species-rich classes of fungi, and highlights for the first time their diversity and phylogenetic relationships with regard both to the endophytes they inhabit and the plants in which these endophyte-bacterium symbiota occur.

Fungal endophytes: isolation and diversity among Nicotiana tabacum (Solanaceae) organs

2020 ◽  
Vol 62 (1-2) ◽  
pp. 175-186
Author(s):  
M. F. Vianna ◽  
S. Pelizza ◽  
M. L. Russo ◽  
S. Rodriguez ◽  
A. C. Scorsetti
Keyword(s):  
Nicotiana Tabacum ◽  
Fusarium Graminearum ◽  
Endophytic Fungi ◽  
Relative Frequency ◽  
Fungal Endophytes ◽  
Fungal Species ◽  
Tobacco Plants ◽  
Fungal Strains ◽  
Starting Point ◽  
Stems And Leaves

The diversity of fungal endophytes is poorly known and particularly in the case of Nicotiana tabacum, the literature is limited. The present study assessed and compared the diversity and distribution of endophytic fungi between different organs of tobacco plants. We calculated the relative frequency and rates of colonisation and of isolation of endophytic fungi in roots, stems and leaves, as well as the Shannon–Wiener and Simpson diversity indexes. Similarities between assemblages from the studied organs were also analysed. A total of 1588 endophytic fungal strains assigned to 31 morphospecies were isolated. The highest diversity of endophytes was found in leaves, being Fusarium graminearum and Alternaria botrytis the most common fungal species. This study provides information on the distribution of fungal endophytes inhabiting leaves, stems, and roots of N. tabacum and thus can serve as a starting point for increasing our comprehension on the interactions in which these fungi are involved.

A New RoVe Method to Purify Endophytic Fungi from Medicinal Plants Western Ghats of Karnataka India

2020 ◽  
Vol 13 (4) ◽  
pp. 5011-5019
Author(s):  
Ankala Basappa Vedamurthy ◽  
Rohit Shankar Mane
Keyword(s):  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Aloe Vera ◽  
Thielaviopsis Basicola ◽  
Surface Sterilization ◽  
Epiphytic Microorganisms ◽  
Sterilization Method ◽  
Successful Isolation ◽  
Aspergillus Nomius

Endophytic fungi from medicinal plants were hardly ever reported as compared to those from soil and marine sources. The present results associate the presence of endophytic fungi in medicinal plants by isolating them from three medicinal plants i.e. Bombax ceiba, Aloe vera, and Ximenia americana. In the present research investigation, surface sterilization method and media were standardized, and 32 endophytic fungi were isolated from three medicinal plants. We assessed the competence of three different surface sterilization methods and four media for isolation of endophytic fungi. The RoVe method used was more effective in eliminating epiphytic microorganisms. Therefore by using new method we have isolated total of 32 fungal endophytes and those were belongs to Aspergillus nomius (63.20%), Aspergillus niger (41.60%), Thielaviopsis basicola (38.33%), Fusarium oxysporum (33.20%), Pestalotiopsis inflexa (27.20%), Nigrospora sphaerica (20%), Alternaria alternata (15.30%) and Phomopsis archeri (20%). This is the first report of successful isolation of endophytic fungi from the said medicinal plants, for using newly formulated surface sterilization method.

Mogroside V-producing Endophytic Fungi Isolated from Siraitia grosvenorii

Planta Medica ◽  
2020 ◽  
Vol 86 (13/14) ◽  
pp. 983-987 ◽  
Author(s):  
Chen Bin ◽  
Yu Fangming ◽  
Jiang Zhi
Keyword(s):  
Endophytic Fungi ◽  
Fusarium Solani ◽  
Internal Transcribed Spacer ◽  
High Performance ◽  
Host Plants ◽  
Morphological Observation ◽  
Its Sequence ◽  
Active Components ◽  
Siraitia Grosvenorii ◽  
Stem Leaf

AbstractEndophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant Siraitia grosvenorii. These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as Diaporthe angelica and Fusarium solani, respectively.

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