scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK KULTUR JAMUR ENDOFIT Fusarium sp. CSP-4 YANG DIISOLASI DARI Curcuma sumatrana Miq.

2020 ◽  
Vol 19 (1) ◽  
pp. 71-76
Author(s):  
Dewi Wulansari ◽  
Ersaliany Nurul Pratiwi Qodrie ◽  
Bodhi Dharma ◽  
Andi Saptaji Kamal ◽  
Lukman Hafid ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Microdilution Method ◽  
Fusarium Sp ◽  
Layer Chromatography ◽  
Weak Antibacterial Activity ◽  
Microwell Plate

Endophytic fungus Fusarium sp. CSP-4 was isolated from the rhizome of Curcuma sumatrana Miq. The ethyl acetate extract of CSP-4 was fractionated by chromatographic column with silica as a stationary phase. The fractions were tested for antibacterial activity against S.aureus and E.coli by Thin Layer Chromatography (TLC)- bioautography. Determination of Minimum Inhibitory Concentration (MIC) was carried out by the microdilution method on a 96-microwell plate with the concentrations from 2 to 256 µg/ml. The results showed that fraction 15 and 11 were able to inhibit bacterial growth with the MIC values of 256 and> 256 μg / mL against S.aureus and>256 μg / mL against E.coli, respectively.The antibacterial activity of fractions 15 and 11 was weaker compared to the positive control of chloramphenicol with the MIC value of 4 µg/mL against S.aureus and E.coli. Based on the results obtained showed that the fractions of the methanol extract of endophytic fungus Fusarium sp. CSP-4 have weak antibacterial activity. 

scholarly journals Antibacterial evaluation of Styrax pohlii and isolated compounds

2013 ◽  
Vol 49 (4) ◽  
pp. 653-658 ◽  
Author(s):  
Camila Spereta Bertanha ◽  
Susane Hellen Utrera ◽  
Valéria Maria Melleiro Gimenez ◽  
Milton Groppo ◽  
Márcio Luis Andrade e Silva ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Preliminary Evaluation ◽  
Microdilution Method ◽  
Aerial Parts ◽  
Broth Microdilution Method ◽  
Weak Antibacterial Activity ◽  
Mic Value

The antibacterial activity of the compounds egonol (1) and homoegonol (2), of the crude ethanolic extract of Styrax pohlii (Styracaceae) aerial parts (EE), and of its n-hexane (HF), EtOAc (EF), n-BuOH (BF), and hydromethanolic (HMF) fractions was evaluated against the following microorganisms: Streptococcus pneumoniae (ATCC 6305), S. pyogenes (ATCC 19615), Haemophilus influenzae (ATCC 10211), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 10031). The broth microdilution method was used for determination of the minimum inhibitory concentration (MIC) during preliminary evaluation of antibacterial activity. The EE yielded MIC values of 400 µg/mL for S. pneumoniae and P. aeruginosa and 300 µg/mL for H. influenzae. The HF and EF fractions exhibited enhanced antibacterial activity, with MIC values of 200 µg/mL against S. pneumoniae, but only EF displayed activity against H. influenzae (MIC 200 µg/mL). The best MIC value with compounds 1 and 2 (400 µg/mL) was obtained for (1) against S. pneumoniae and P. aeruginosa. Therefore, 1 exhibited weak antibacterial activity against these standard strains.

scholarly journals ANTIBACTERIAL ACTIVITY OF FLAVONOID FROM KEPEL (STELECHOCARPUS BURAHOL) LEAVES AGAINST STAPHYLOCOCCUS EPIDERMIDIS

2017 ◽  
Vol 9 (10) ◽  
pp. 292
Author(s):  
Susi Indariani ◽  
Aisyah Hidayat ◽  
Latifah K. Darusman ◽  
Irmanida Batubara
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Functional Group ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Preparative Thin Layer Chromatography ◽  
Active Fraction ◽  
Microdilution Method ◽  
Layer Chromatography

Objective: The objective of this research was to separate flavonoids of kepel (Stelechocarpus burahol) leaves from methanol extract as antibacterial agent against Staphylococcus epidermidis that have a role in body odor.Methods: The methanol extracts of kepel was fractionated in methanol: water (7:3), n-hexane and chloroform consecutively. Methanol: water (7:3) extract with the highest flavonoids content, fractioned by silica gel column chromatography (isocratic elution, with n-buthanol: methanol: acetic acid (1:8:1) as eluent) to produce 7 fractions. All fractions were tested for antibacterial activity with a microdilution method. The most active fractions was determined using UV-VIS spectrophotometer (Shimadzu, Japan) and FTIR (Brucker, Germany).Results: Fraction V was the most active fraction with minimum inhibitory concentration (MIC) 0.06 mg/ml and minimum bactericidal concentration (MBC) of 0.50 mg/ml. Fraction V was further separated by preparative thin layer chromatography (TLC) and gave three fractions. Fraction V3 was the most active fraction with MIC 1.00 mg/ml and MBC of 2.00 mg/ml. Identification of fraction V3 based on assessments on ultraviolet-visible and infrared spectrum showed the maximum wavelength at 327 nm. These results indicate a transition π → π * and n → π * generated from the chromophore conjugated C = C and C = O. Based on the allegations of the functional group obtained, then alleged that in Fraction V3 containing flavones compounds.Conclusion: These results suggest that flavonoid of S. burahol leaves extracts is potential as antibacterial agents against S. epidermidisand therefore justifies their usage in traditional medicine for the treatment of body odor. 

scholarly journals Determination of MIC, MPC, and MSW of Ilex paraguariensis Against Non-Typhoidal Salmonella with Identification of the Mechanisms of Resistance and Pathogenicity Factors

2020 ◽  
Author(s):  
Khaled El Khatib ◽  
Ribal Aby Hadeer ◽  
Anis Saad ◽  
Aline Kalaydjian ◽  
Elie Fayad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ilex Paraguariensis ◽  
Salmonella Spp ◽  
Mutant Selection ◽  
Selection Window ◽  
Mutant Prevention Concentration ◽  
Resistance Patterns ◽  
Different Strains

Abstract Objective: This study investigated the antibacterial activity of Ilex paraguariensis extracts against 32 different strains of non-typhoidal Salmonella (NTS) through the determination of the Minimum Inhibitory Concentration (MIC), Mutant Prevention Concentration (MPC), Mutant Selection Window (MSW), and the detection of virulence genes by multiplex PCR assays. Results: The MIC values of Ilex paraguariensis against Salmonella spp. strains varied between 0.78 mg/ml and 6.25 mg/ml with a MIC 90 of 3.12 mg/ml. The highest MPC in this study was 48 mg/ml yielding a Mutant Selection Window of 41.75 mg/ml. The MSW values of the remaining strains varied between 1.56 and 8.87 mg/ml. Genes of pathogenicity detected in Salmonella spp. isolates were most commonly the stn, sdiA, invA, sopB, invH, and sopE genes. The antibacterial activity of Yerba Mate extracts was not affected by the antimicrobial resistance patterns or pathogenicity genes expressed. More work is needed to identify the active antibacterial compound(s) responsible for the antibacterial activity.

scholarly journals Synthesis, antimicrobial activity, and determination of the lipophilicity of ((cyclohex-3-enylmethylene)hydrazinyl)thiazole derivatives

2019 ◽  
Vol 28 (11) ◽  
pp. 2023-2036 ◽  
Author(s):  
Anna Biernasiuk ◽  
Magdalena Kawczyńska ◽  
Anna Berecka-Rycerz ◽  
Beata Rosada ◽  
Anna Gumieniczek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Positive Control ◽  
Reversed Phase ◽  
Thiazole Derivatives ◽  
Strong Activity ◽  
Cytotoxicity Studies ◽  
L929 Fibroblast ◽  
Layer Chromatography

Abstract Synthesis and investigation of antimicrobial activity of fifteen novel thiazoles containing cyclohexene moiety are presented. Among the derivatives, compounds 3a–3d, 3f, 3n, and 3o showed very strong activity against the reference Candida spp. strains with MIC = 0.015–3.91 µg/ml. The activity of these compounds is similar and even higher than the activity of nystatin used as positive control. Compounds 3d, 3f, 3n, 3o showed the highest activity with very strong effect towards most of yeasts isolated from clinical materials with MIC = 0.015–7.81 µg/ml. The cytotoxicity studies for the most active compounds showed that Candida spp. growth was inhibited at noncytotoxic concentrations for the mammalian L929 fibroblast. In addition, a good correlation was obtained between lipophilicity of compounds determined using reversed phase thin-layer chromatography and their antifungal activity.

scholarly journals ANTIBACTERIAL ACTIVITY AGAINST BACILLUS SUBTILIS AND ANTIOXIDANT PROPERTIES OF METHANOL EXTRACTS FROM GARCINIA LATISSIMA MIQ. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 24 ◽  
Author(s):  
Neneng Siti Silfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhammad Hanafi ◽  
Hanita Omar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Wide Spectrum ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Inhibition Zones

Objective: The objective of this study was to identify fractions with the highest antibacterial activity against Bacillus subtilis and to determineantioxidant activities and establish the chromatographic fractions as candidate antibacterial and antioxidant agents.Methods: Extracts were fractionated using column chromatography, and antibacterial activities were assayed by the analyses of inhibition zones andbioautography, as well as by broth microdilution techniques. Antioxidant activities were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH)assay.Results: The strongest antibacterial activity against B. subtilis (ATCC 6633) was observed with fractions B and C obtained in this research, witha minimum inhibitory concentration value of 312.5 μg/mL. The effective percentage (EP) value of crude extract at 10 μg/mL was 29.47±2.01%.Fractions C and D had greater EP values than the crude extract, whereas fraction D had the highest scavenging activity against DPPH free radicals(37.73±1.44%) when used at 10 μg/mL. The half effective concentration of the extract was 23.40 μg/mL, whereas that of the most active fraction Dwas 19.38 μg/mL and quercetin as positive control was 3.72 μg/mL.Conclusion: The present data confirm that fractions of methanol extract from Garcinia latissima Miq. leaves possess antibacterial and antioxidantactivities. These observations may facilitate the development of antimicrobial phytomedicines with a wide spectrum of activities and standardizedantioxidant properties.

scholarly journals FRAKSI ETANOL RIMPANG TEMU PUTIH (Curcuma zedoaria (Christm.) Rosc.) SEBAGAI ANTIFUNGI TERHADAP Candida albicans dan Trichophyton rubrum

2019 ◽  
Author(s):  
Yuli Wahyu Trimulyani ◽  
Nurma Suri ◽  
Niken Dwi Astarina
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Traditional Medicine ◽  
Inhibitory Concentration ◽  
Trichophyton Rubrum ◽  
Ethanol Extract ◽  
Positive Control ◽  
Curcuma Zedoaria ◽  
Synthetic Drugs ◽  
Layer Chromatography

Traditional medicine is used as natural because it has small effects and more economical than the synthetic drugs. One example of the plants used as a Traditional medicine are Curcuma zedoaria. The study to examine the activity fraction of Curcuma zedoaria rhizome ethanol extract agains Candida albicans and Trichophyton rubrum. The simplisia of Curcuma zedoaria rhizome was extracted with ethanol 70% using a maceration method. Thick extracts were then fractionated using saveral solven such as n-heksane, chloroform and ethanol. The testing method used disc antifungi activity with the concentration of 6.25%, 12.5%, 25%, 50%, 75%, ketokonazol as the positive control and aquades as the resolvent. The test compounds Thin Layer Chromatography (TLC) method with eluen chloroform: methanol: water (2:3:5(v/v/v)) and phase uses G60F254 had been done. The result showed that Curcuma zedoaria rhizome significantly inhibit of C. albicans and T. rubrum the best inhibit zone diameter that are concentration of 75% for both of them with a diameter 8.98 mm in C. albicans and 11.66 mm in T. rubrum. Minimum Inhibitory Concentration (MIC) C. albicans is obtained on concentration of 6.25% and T. rubrum is concentration on of 12.5%. The TLC results showed a compound of flavonoids (Rf 0.88), saponins (Rf 0.74), tannins (Rf 0.86). Minimum Inhibitory Concentration (MIC) in C. albicans and T. rubrum are fungistatik and Minimum Bactericidal Concentration (MBC) are not fungisid for both of them. The ethanol fraction of Curcuma zedoaria rhizome has an activity as an antifungal against C. albicans and T. Rubrum with flavonoids, tannins and saponins. Keywords: Antifungi, Candida albicans, Curcuma zedoaria, Fungistatic, Trichophyton rubrum.

scholarly journals PROFIL KANDUNGAN KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAMBAN (Donax canniformis (G. Forst.) K. Schum.) TERHADAP Staphylococcus aureus

2015 ◽  
Vol 1 (2) ◽  
pp. 141-148
Author(s):  
Hidayatullah Hidayatullah ◽  
Syariful Anam ◽  
Muhamad Rinaldhi Tandah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Dilution Method ◽  
The Family

Bamban (Donax canniformis (G. Forst.) K. Schum.) is one of the family Marantaceae plant that has many uses such as traditional medicine. Methanol extract of bamban leaves contains phenolic, tannins and saponins compounds. The purpose of this research is to determine the class of compounds that has antibacterial activity against Staphylococcus aureus and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methanol extract of bamban leaves. This extract was prepared using maceration method with methanol solvent. Determination the class of compounds was initiated by bioautografi test in order to determine spots which has have antibacterial activity. Subsequently, the spot were identified the class of compound using reagent spray FeCl3 and H2SO4 10%. The determination of MIC and MBC using dilution method. Research showed there are three compounds that had antibacterial activity. These compounds were predicted as spot I and spot II which were phenolic compounds and spot III as a saponin compound. MIC and MBC value of the methanol extract of leaves bamban leaves 8% and 13%, respectively.

scholarly journals KAJIAN FRAKSI METANOL DARI EKSTRAK METILEN DIKLORIDA KULIT KAYU BATANG PELAWAN (Tristania whitiana Griff.) SEBAGAI ANTIBAKTERI

Molekul ◽  
2007 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Purwantiningsih Sugita
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inhibitory Concentration ◽  
Methanol Fraction ◽  
The Third ◽  
The One ◽  
Layer Chromatography ◽  
Chloroform Methanol

Antibacterial compound from methanol fraction of methylene dichloride extract of pelawan bark had been studied. Pelawan bark was extracted using ethanol 95% and CH2Cl2-water (1:1), respectively. Extract of CH2Cl2 was partitioned using hexane-methanol 90% (1:1). Afterwards, methanol fraction was analyzed by thin layer chromatography methods to find the best eluent for column chromatography. The best eluent came from mixture of acetone-chloroform-methanol (0,6:4:0,4). The fractionation yielded 16 fractions with the first (M1) and the third (M5) fractions became the one spotted fractions. M1 and M5 fractions were examined for their antibacterial activity using paper diffusion methods by determining minimum inhibitory concentration value. M1 fraction showed greater activity than M5 fraction with minimum inhibitory concentration value of 2,7857 mg/mL. The result of phytochemistry test showed that both fractions contain terpenoid.

scholarly journals Antibacterial and antioxidant activities of Licania tomentosa (Benth.) fritsch (crhysobalanaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 459-464 ◽  
Author(s):  
J.B.N.F. Silva ◽  
I.R.A. Menezes ◽  
H.D.M. Coutinho ◽  
F.F.G. Rodrigues ◽  
J.G.M. Costa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Antioxidative Capacity ◽  
Bacterial Strains ◽  
Hydroalcoholic Extract ◽  
Phytochemical Composition ◽  
Antibacterial Assay

This work describes the chemical composition, and evaluates the antimicrobial and antioxidant activities of a hydroalcoholic extract from the leaves of the Licania tomentosa. Gram positive and negative bacterial strains were used in this work. Examination of the phytochemical composition of L. tomentosa revealed the presence of secondary metabolites such as tannins, flavonoids, saponins, alkaloids, steroids and triterpenoids. An antibacterial assay pointed out that the extract had a lower minimal inhibitory concentration (MIC - 32 ?g/mL) towards Staphylococcus aureus (ATCC12692). The extract also presented antibacterial activity against other assayed bacteria, with the MIC varying between 64 and 512 ?g/ mL. Our findings reveal that the extract presented an antioxidative capacity lower than that of BHT at the same concentration, used as positive control. Our results suggest that the levels and combinations between the secondary metabolites of this plant should be investigated to explain the demonstrated antibacterial activity.

Sign in / Sign up

Export Citation Format

Share Document