Evaluation of an HPLC Method for Quality of Fructus of Ligustrum lucidum Ait Pieces

Background: This study was aimed to determine the frequency of thalassemia and Hb variant in blood donor. In addition, we also wanted to know the quality of blood from the donor up to seven days of storage, by calculating percentage of hemolysis in vitro.Methods: This cross-sectional study was conducted on 138 blood donor specimens at Red Cross Blood Centre Unit in Jakarta. All specimens were tested for thalassemia and Hb variant by complete blood count (CBC) and Hb analysis with HPLC method and DNA analysis for the detection of α thalassemia carrier. To analyze the quality of stored blood, the calculation of hemolytic rate of red blood cells (RBCs) on whole blood (WB) was compared between the first and seventh days of storage.Results: Out of the 138 specimens, 5 samples (3.6%) were diagnosed for α thalassemia carrier in which, one of them is co-inherited with ovalositosis hereditary (Southeast Asian Ovalositosis/SAO), 3 samples (2.2%) for β thalassemia carrier, and 3 samples (2.2%) for Hb E. Meanwhile, the hemolytic rates of RBCs on WB in first day and seven day of storage were below one percent.Conclusion: The frequency of thalassemia carrier and Hb variants in blood donors at Red Cross Blood Centre Unit in Jakarta was 8%. The quality of stored blood until seven day of storage was quite good.

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Profiling of primary metabolites in grapes of interspecific grapevine varieties: sugars and organic acids

Czech Journal of Food Sciences ◽

10.17221/257/2010-cjfs ◽

2011 ◽

Vol 29 (No. 4) ◽

pp. 361-372 ◽

Cited By ~ 14

Author(s):

P. Pavloušek ◽

M. Kumšta

Keyword(s):

Cluster Analysis ◽

Organic Acids ◽

Vitis Vinifera ◽

Hplc Method ◽

The Other ◽

Vitis Vinifera L ◽

Primary Metabolites ◽

Primary And Secondary Metabolites ◽

The Individual

The quality of grapes is determined above all by the contents of the primary and secondary metabolites. The primary metabolites involve sugars and organic acids, and just these compounds are dealt with in this study. Its objective was to analyse and critically evaluate the primary metabolites in new interspecific varieties and, based on a comparison with European varieties of grapevine (Vitis vinifera L.), to find out the similarities and also possible differences between them. The study evaluates and compares 4 conventional varieties of Vitis vinifera with 11 new interspecific cultivars. The contents and compositions of the individual sugars and acids were estimated by means of the HPLC method. Most of these varieties belong to the group with either medium or low content of malic acid, i.e. with a medium to high β ratio. This corroborates the similarity of interspecific varieties to those of V. vinifera. The cluster analysis identified the existence of two interesting groups of varieties: the first one involved the varieties Riesling, Nativa, Marlen, and Kofranka while the other group consisted of varieties Blaufränkisch, Blauer Portugieser, and Laurot. This observation also indicates similarity between Vitis vinifera L. varieties and interspecific cultivars and demonstrates that the contents of the primary metabolites (i.e. sugars and organic acids) are also comparable.

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Quantitative and Qualitative Analysis of Aconitum Alkaloids in Raw and Processed Chuanwu and Caowu by HPLC in Combination with Automated Analytical System and ESI/MS/MS

Journal of Analytical Methods in Chemistry ◽

10.1155/2012/936131 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Aimin Sun ◽

Bo Gao ◽

Xueqing Ding ◽

Chi-Ming Huang ◽

Paul Pui-Hay But

Keyword(s):

Qualitative Analysis ◽

Hplc Method ◽

Chinese Herbs ◽

Herbal Preparations ◽

Esi Ms ◽

Aconitum Alkaloids ◽

Analytical System ◽

Rapid Tool

HPLC in combination with automated analytical system and ESI/MS/MS was used to analyze aconitine (A), mesaconitine (MA), hypaconitine (HA), and their benzoyl analogs in the Chinese herbs Caowu and Chuanwu. First, an HPLC method was developed and validated to determine A, MA, and HA in raw and processed Caowu and Chuanwu. Then an automated analytical system and ESI/MS/MS were applied to analyze these alkaloids and their semihydrolyzed products. The results obtained from automated analytical system are identical to those from ESI/MS/MS, which indicated that the method is a convenient and rapid tool for the qualitative analysis of herbal preparations. Furthermore, HA was little hydrolyzed by heating processes and thus it might account more for the toxicity of processed aconites. Hence, HA could be used as an indicator when one alkaloid is required as a reference to monitor the quality of raw and processed Chuanwu and Caowu. In addition, the raw and processed Chuanwu and Caowu can be distinguished by monitoring the ratio of A and MA to HA.

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Determination of polyphenol and EGCG in tea and tea products by UV-VIS and HPLC methods

Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam ◽

10.47866/2615-9252/vjfc.47 ◽

2018 ◽

Vol 1 (1) ◽

pp. 8-12

Author(s):

Ngoc Mai Pham Thi ◽

Binh Le Thai ◽

Dong Pham Huy ◽

Tuyet Nhung Nguyen Thi ◽

Duc Pham Tien ◽

...

Keyword(s):

Active Ingredient ◽

Analytical Methods ◽

Hplc Method ◽

Total Phenol ◽

Total Polyphenol ◽

Active Substances

Tea and tea products have been known and used for long and became a tradional culture in many countries in the world, including Vietnam. The quality of tea depends on the concentrations of typical active substances such as polyphenol groups and their active substances (EGCG, ECG, catechin,…). Therefore, study of analytical methods to determine these active substances is necessary to evaluate the quality of tea and tea products. In this study, UV-Vis method was selected to determine total polyphenol and HPLC method was selected to determine the main active ingredient of polyphenol group EGCG. The limit of detections are 0.12 mg/g and 0.064 mg/g for total polyphenol and EGCG, respectively. These methods were applied to determine the concentrations of total phenol and EGCG in 10 samples of tea and tea products. Results show that the concentrations of total phenol and EGCG vary significantly among samples, but there is a certain relationship between the concentration of total phenol and EGCG in each sample.

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Ocena zawartości metyloglioksalu w miodzie manuka znajdującym się na rynku krajowym

Postępy Fitoterapii ◽

10.25121/pf.2018.19.2.106 ◽

2018 ◽

Vol 19 (2) ◽

Author(s):

Deta Łuczycka ◽

Maciej Olczyk

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Hplc Method ◽

Photometric Method ◽

Manuka Honey ◽

Promoting Effect ◽

Health Promoting ◽

Human Digestive Tract

Introduction. Recently, mass media published a number of articles doubting health promoting effect of manuka honey (MH). On the other hand, many researchers emphasize beneficial effect of honey on human digestive tract, respiratory system and skin. However, the honeys tested for their health promoting effects came from certified sources and met the applicable harvesting, storage and packing standards between the production and testing. Aim. The purpose of the study was to compare quality features of manuka honey (MH) imported to and sold in, Poland: methylglyoxal (MGO), 5-hydroxymethylfurfural (HMF) and diastase number (DN) declared for product lots by producers and distributors vs. the actual figures. Material and methods. The study tested 45 samples of MH coming from 9 producers, acquired from different local suppliers (importers and distributors), marketed in Poland in 2014-2015. The contents of MGO were measured using the high-performance liquid chromatography (HPLC) method. The contents of HMF were measured using high-performance liquid chromatography with spectrophotometric detection (HPLC-UV) in accordance with the regulation by the Polish Minister of Agriculture. DN were determined using the photometric method acc. To the regulation by the Polish Minister of Agriculture. Results. A review of the test results revealed a relationship between the declared and the actual contents of MGO. For the MH with MGO > 500, the actual values were different more frequently. For the MH with higher declared MGO contents (< 500), commercial quality of the products was worse (too high HMF and too low DN). Conclusions. The MH with MGO under 500 mg/kg can be recognized as satisfactory in terms of MGO contents and commercial quality.

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Effect of Bagging on the Content of Sugar and Acid in Postharvest "Jinhuang" Mango Fruit

E3S Web of Conferences ◽

10.1051/e3sconf/202014501032 ◽

2020 ◽

Vol 145 ◽

pp. 01032

Author(s):

Changbin Wei ◽

Jian Qiao ◽

Xinming Tang ◽

Qingze Yan ◽

Lizhu Tang ◽

...

Keyword(s):

Ascorbic Acid ◽

Citric Acid ◽

Organic Acids ◽

Fruit Quality ◽

Hplc Method ◽

Postharvest Storage ◽

Mango Fruit ◽

Acid Ratio

The glucose, fructose, sucrose, and organic acids in the pulp of “Jinhuang” mango were analyzed using the HPLC method and the effect of bagging on fruit quality was researched during the postharvest storage. The results showed that there was a certain difference in the content of sugar and acid among the three treatments. The effect of bagging treatments on fructose, glucose, and sucrose in the fruit of “Jinhuang” mango was mainly reflected in sucrose. The treatments of white bagging (WB) and black bagging (BB) delayed the production of sucrose and the decomposition of citric acid, and increased the content of ascorbic acid and quinine. Based on sugar-acid ratio, the flavor of the three treatments was evaluated as: CK> WB> BB. The fruit quality of “Jinhuang” mango was affected by bagging treatments to a certain extent.

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HPLC Fingerprint Combined With Multicomponent Quantification as an Efficient Method for Quality Evaluation of Pharbitidis Semen

Natural Product Communications ◽

10.1177/1934578x20931642 ◽

2020 ◽

Vol 15 (6) ◽

pp. 1934578X2093164

Author(s):

Huiwei Bao ◽

Huailei Yang ◽

Feng Wang ◽

Kaixuan Zhou ◽

Yanan Yang ◽

...

Keyword(s):

Cluster Analysis ◽

Caffeic Acid ◽

Evaluation System ◽

High Performance ◽

Quality Evaluation ◽

Hplc Method ◽

Hplc Fingerprint ◽

Protocatechuic Aldehyde ◽

Effective Components

Pharbitidis Semen is a traditional Chinese medicine(TCM) with a long history for treatment of edema and fullness, fecal and urinary retention, phlegm and retained fluid, and abdominal pain due to parasitic infestation. Since Pharbitidis Semen is distributed throughout the country, the quality of the medicine from different origins may be varied. Moreover, the reported method could not control the quality comprehensively. In this article, a fingerprint of Pharbitidis Semen has been established based on a high-performance liquid chromatography (HPLC) method. In addition, the contents of the 2 main effective components were determined simultaneously. The reference HPLC fingerprint was obtained according to the chromatograms of test samples. The similarity values were calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A edition). Cluster analysis of 10 batches of samples was performed using statistical software (SPSS 20.0). The HPLC fingerprints of 10 batches of Pharbitidis Semen showed 25 well-resolved common peaks in each chromatogram. Two of these peaks were assigned to protocatechuic aldehyde and caffeic acid. As a result, HPLC fingerprint similarities of 10 batches of samples were more than 0.99. Pharbitidis Semen from different habitats could be divided into 3 or 2 groups. The results of cluster analysis showed that samples classified into 1 group were associated with their habitats and breeds. At the same time, quantification results showed that the contents of protocatechuic aldehyde and caffeic acid were in the range of 0.026-0.088 and 0.019-0.053 mg/g−1 respectively. HPLC fingerprint combined with multicomponent quantification and data analysis techniques can be an efficient and useful method for monitoring the quality of Pharbitidis Semen. This study also provides a practical strategy for overall quality evaluation and control of traditional Chinese medicines.

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Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol

Indonesian Journal of Chemistry ◽

10.22146/ijc.63730 ◽

2021 ◽

Author(s):

Christofori Maria Ratna Rini Nastiti ◽

Florentinus Dika Octa Riswanto

Keyword(s):

Limit Of Detection ◽

Aqueous Solubility ◽

Reversed Phase ◽

Hplc Method ◽

Optimum Composition ◽

Analytical Method Validation ◽

Limit Of Quantitation ◽

Diabetic Wounds

Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability. To overcome the issues, RSV was formulated as a topical nanoemulsion. It is important to ensure the quality of the dosage form by evaluating RSV load in the nanoformulation and optimizing the formula. A reversed-phase HPLC method was developed and validated prior to the load determination of RSV in the nanoemulsion formulation. The composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® was further optimized by employing a Box-Behnken Design (BBD) to achieve the optimum composition with expected viscosity and RSV load. The HPLC method for determining RSV load was successfully validated for parameters of selectivity with the resolution of 8.487, linearity and range (r = 0.9979), precision (0.12% of RSD), accuracy (109–110% of recovery), the limit of detection (0.574 µg/mL), and limit of quantitation (1.740 µg/mL). The result of formula optimization was promising, showing the optimum composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® at 4.44 g, 30.97 g, and 11.39 g, respectively.

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Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269

Pharmacia ◽

10.3897/pharmacia.68.e63457 ◽

2021 ◽

Vol 68 (2) ◽

pp. 427-431

Author(s):

Zoya Shprakh ◽

Andrei Budko ◽

Dmitry Kozin ◽

Lydia Ektova ◽

Dmitry Gusev ◽

...

Keyword(s):

Hplc Method ◽

Intermediate Precision ◽

National Medical ◽

Quantitation Limit ◽

Mobile Phases ◽

Precision Estimation ◽

The Mean ◽

Development And Validation ◽

The Relationship

Indolocarbazole glycosidic derivative LCS-1269 with significant antiproliferative activity has been synthesized in N.N. Blokhin National Medical Research Center of Oncology. To control the quality of the substance, the chromatographic method of the assay was created and validated. The technique was carried out in a gradient mode using mobile phases consist of acetonitrile, trifluoroacetic acid and purified water. The specificity of the method was shown by checking of test solutions and the special solvent chromatograms. The method linearity was confirmed, and the parameters of linear dependence have been estimated, and the relationship was described by the equation: y = 49.23× – 35.51 with correlation coefficient 0.9998. The method’s precision was determined as the repeatability with a relative error of the mean 1.49% and was 2.433 ± 0.036. Was shown, that the results obtained in the intermediate precision estimation were not burdened with a systematic error. The detection limit and quantitation limit were calculated based on the linear relationship data as 3.15 μg/mL and 9.57 μg/mL, respectively. Sensitive HPLC method for LCS-1269 assay in substance has been developed and validated.

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Quality evaluation of simvastatin compounded capsules

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502011000300007 ◽

2011 ◽

Vol 47 (3) ◽

pp. 495-502 ◽

Cited By ~ 1

Author(s):

Flávia Dias Marques-Marinho ◽

José Carlos da Costa Zanon ◽

Emilia Sakurai ◽

Ilka Afonso Reis ◽

Angélica Alves Lima ◽

...

Keyword(s):

Treatment Efficacy ◽

Quality Evaluation ◽

Quality Standards ◽

Hplc Method ◽

Average Weight ◽

Content Uniformity ◽

Dissolution Test ◽

Test Conditions ◽

Weight Variation

Simvastatin is commercially available as tablets and compounded capsules in Brazil. Very few reports regarding these capsules' quality, and consequently their efficacy, are available. The pharmaceutical quality of 30 batches of 20 mg simvastatin capsules from the market was evaluated by weight determination, content uniformity, disintegration (Brazilian Pharmacopeia), assay and dissolution test (USP32 tablet monograph). A HPLC method was developed for assay, content uniformity and dissolution test, and specifications were also established. Out of the 30 batches evaluated, 29 showed capsule disintegration within 45 min and individual weight variation was within ±10% or ±7.5% relative to average weight, for < or > 300 mg, respectively. Only 27 batches met dissolution test criteria with values >80% of the labeled amount in 45 min; 21 batches showed simvastatin content between 90.0-110.0% of the labeled amount and 19 batches had at least 9 out of 10 capsules with content uniformity values between 85.0-115.0% of the labeled amount with RSD<6.0%. Only 14 of all (30) batches fully met pharmacopeial quality standards. The establishment of test conditions and specification parameters for simvastatin capsules showed that there are relevant pharmacopeial quality differences between batches compounded by different pharmacies. For 53.33% of the tested products hypercholesterolemic treatment efficacy may be compromised.

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