scholarly journals INHIBITION OF PHOSPHATIDYLCHOLINE LIPOSOMES OXIDATION BY PHENOLIC COMPOUNDS OF ALOE EXTRACTS: A. ARBORESCENS, A. PILLANSII AND A. SQUARROSA

2019 ◽  
pp. 83-90 ◽  
Author(s):  
Natalia Nikolaevna Sazhina ◽  
Petr Vladimirovich Lapshin ◽  
Natal'ya Viktorovna Zagoskina ◽  
Nadezhda Pavlovna Palmina

Various species of the genus Aloe which there are in the world more than 500 are considered as important sources of biologically active substances and attract an attention of researchers by numerous manifestations of their biological properties. The most studied and used Aloe species are A. arborescens and A. vera, however some other species show not smaller biological activity, in particular antioxidant, than made mention. In the present work on model of the initiated oxidation of phosphatidylcholine liposomes comparison of the antioxidant activity (AOA) of leaves extracts of A. arborescens, A. pillansii, A. squarrosa and also the total content of their phenolic compounds was carried out. It is established that A. pillansii extract has approximately by 12 times higher AOA, than A. arborescens extract, and by 4 times, than A. squarrosa. The measured values of the total phenolic compounds content showed considerably smaller difference between these extracts. It can demonstrate existence in A. pillansii leaves of more difficult antioxidant profile, than in A. arborescens, and higher concentration of active phenolic metabolites. Results of this work allow recommending A. pillansii for more depth studies of its different biological activities.

2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Jaunė Blažytė ◽  
Nijolė Vaitkevičienė

The aim of this work was to investigate and compare the amounts of biologically active compounds, dry matter and soluble solids in pulp and peels of four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’). The research was carried out at Vytautas Magnus University Agriculture Academy (Lithuania) in 2020. Four pear cultivars (‘Ksena’, ‘Beloruskaja pozdniaja’, ‘Alna’ and ‘Aleksandr Lucas’) were cultivated at a farm in the Joniškis District of Lithuania. Pears were grown following traditional pear production technology. The amounts of dry matter, soluble solids, vitamin C and fiber were determined using the standard method. The total content of phenolic compounds was determined using a Folin-Ciocalteu reagent with a UV/VIS spectrophotometer. The research results showed that significantly the highest amounts of soluble solids were found in the ‘Alna’ pear fruit pulp (14.25% f. w.). The ‘Alna’ peel sample had significantly the highest amount of total phenolic compounds (1290.99 mg 100 g–1 d. w.). The highest content of dry matter was found in the ‘Aleksandr Lucas’ pear fruit peel (24.67%). The ‘Aleksandr Lucas’ and ‘Beloruskaja pozdniaja’ pear fruit peel contained significantly the highest amounts of vitamin C (2.70 and 2.63 mg 100 g–1 f. w., respectively). The highest content of fiber was found in the ‘Ksena’ fruit peel (36.12% d. w.). The investigated pear peel sample had significantly higher amounts of dry matter, vitamin C, total phenolic compounds and fiber than the pulp. However, the pulp contains the highest contents of soluble solids.


Author(s):  
Nyamdemberel Tsagaanbaatar ◽  
Chimedragchaa Chimedtseren

Traditional prescription Lonlunsemberu-13 has been widely used in traditional Mongolian medicine to treat gastro intestinal dyspepsia. The purpose of this study was to determine biologically active substances in the Traditional prescription Lonlunsemberu-13 as a primary research. We screened for phenolic compounds, flavonoids, coumarins and alkaloids using a TLC method, and then we have determined those biologically active substances content by the UV/Vis spectrophotometric method. We identified the gallic acid, rutin, quercetin, apigenin, luteolin, isofraxidin and piperine in the traditional prescription. The result revealed that “Lonlunsemberu-13” consisted 4.38% ±1.9 of total phenolic compounds, 0.63% ±0.17 flavonoids, 2.45% ±0.28 of coumarins and 0.83% ±0.064 alkaloids.


2008 ◽  
Vol 25 (No. 6) ◽  
pp. 325-332 ◽  
Author(s):  
M. Kováčová ◽  
E. Malinová

Ferulic and coumaric acids were determined by high-performance liquid chromatography in 21 oat genotypes supplied from the gene bank of the Research Institute of Plant Production in Piešťany (Slovak Republic) with the aim of selecting some genotypes suitable for the preparation of functional foods. The content of coumaric acid was higher than that of ferulic acid in 61.9% of samples. The latter acid exceeded proportionally the former one in genotypes with a low content of phenolic acids, namely in the amount of up to 50 mg/100 g of grain, especially in chaffless oats. Ferulic acid content varied in the range from 16.50 mg/100 g (cultivar Jakub) to 149.36 mg/100 g of grain (cultivar Izak). The content of coumaric acid ranged between 8.05 mg/100 g (cultivar Detvan) and 210.27 mg/100 g of grain (cultivar Izak). The fact that the samples were grown in the same year (2003) in one locality (Vígľaš-Pstruša, Slovak Republic) minimises the influence of soil and climatic conditions and proves that remarkable differences between the content of ferulic and coumaric acids and the total content of phenolic compounds were caused by the genetic outfit of oats. A high correlation was found between the contents of the total phenolic compounds, ferulic acid, coumaric acid and their sum. The corresponding correlation coefficients R had the values of 0.9229, 0.9141, and 0.9211, respectively. This correlation enables one to employ a simple and inexpensive method for the determination of the total phenolic compounds using Folin-Ciocalteu reagent for a rapid assessment of differences detectable in the content of the sum of ferulic and coumaric acids in oat samples in view of the selection of genotypes suitable for the preparation of functional foods.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
V. R. Snehalatha ◽  
A. R. Rasmi

Abstract Background The plants belonging to the genus Syzygium has received much attention since ancient times due to its multidimensional uses for mankind. These plants in general, reported to contain a wide array of phytomolecules with a broad spectrum of biological activities that include antihypoglycemic, antioxidant, anti-inflammatory, anticarcinogenic, and antihypertensive properties. The pharmacological potential of S. palghatense which is endemic to Western Ghats has not yet been investigated. The present study was intended to examine the potential benefits of the leaves and bark of S. palghatense. Results Morpho-anatomical studies of S. palghatense provided useful details for the identification. The pharmacological characterization of the plants shows significant results in their studied parameters. The total phenolic and flavonoid contents were higher in methanolic extracts of leaves and bark of S. palghatense. Methanolic extract of the leaves and bark have superior antioxidant capacity when compared with the chloroform, ethyl acetate, and distilled water extracts. A significant result in alpha amylase and alpha glucosidase enzyme activity was exhibited when antidiabetic study was performed. Conclusion The current study showed that the methanolic extract of S. palghatense leaves and bark have significant pharmacognostic properties and potential antioxidant and antidiabetic compounds. Phytochemical studies portray the presence of several biologically active secondary metabolites, including essential oil, alkaloid, glycosides, carbohydrate, tannins, reducing sugar, phenolics, and saponins, which may be the reason for its biological properties. As compared to bark, leaves of S. palghatense possess significant activity toward all the biological properties tested.


2019 ◽  
pp. 127-137 ◽  
Author(s):  
Любовь (Lyubov') Николаевна (Nikolaevna) Скрыпник (Skrypnik) ◽  
Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova)

The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic acid) radical and FRAP (ferric reducing antioxidant power) assays. It was established that the fruits of plants of the genus Sambucus L. were characterized by the maximum level of all studied antioxidants. High content of anthocyanins and phenolic compounds was determined in the leaves of elderberry and dwarf elder. The flowers of these elderberry species were distinguished by a high total content of water-soluble antioxidants. The highest antioxidant activity was observed in fruits extracts in comparison with other parts of the plant. Higher antioxidant activity was identified in the extract of the fruits of elderberry and dwarf elder than of the red elderberry fruits. The most optimal method for evaluating the antioxidant activity of elder extracts was the FRAP assay, which showed the highest correlation between AOA and individual antioxidant components, compared to DPPH and ABTS assays. Comparative analysis of antioxidant content and antioxidant activity of various plant parts of three elderberry species showed that the most promising sources of biologically active substances with antioxidant properties are fruits and flowers of elderberry and dwarf elder.


Weed Science ◽  
2016 ◽  
Vol 64 (1) ◽  
pp. 191-200 ◽  
Author(s):  
Neama A. A. Gouda ◽  
Mona M. G. Saad ◽  
Samir A. M. Abdelgaleil

Monoterpenes, the major constituents of essential oils, are known for their diverse biological activities. This study was conducted to assess the herbicidal effect of six monoterpenes viz. (R)-carvone, 1,8-cineole, cuminaldehyde, (S)-fenchone, geraniol, (S)-limonene, and (R)-linalool on barnyardgrass under laboratory and glasshouse conditions with a view to explore the possibility of their utilization for future weed management. The effect of monoterpenes on chlorophyll contents and total phenolic compounds was also evaluated. The inhibitory effects of monoterpenes on seed germination and seedling growth were tested at concentrations of 1, 2, 4, 6, and 8 mM. The results showed that geraniol and (R)-carvone caused greatest reduction of seed germination with complete inhibition at the concentrations > 2 mM. Similarly, these two compounds were the most potent inhibiters for root and shoot growth. In general, monoterpenes were less effective against seed germination than seedling growth. Furthermore, the inhibition of root growth by all compounds was greater than that of shoot growth. In foliar application treatments under glasshouse conditions, the monoterpenes reduced the fresh and dry weights, and shoot length of two-leaf stage barnyardgrass at concentrations of 1 and 2%. In addition, the tested monoterpenes caused phytotoxicity symptoms, mainly chlorosis and necrosis, followed by weed death. Complete weed control was observed in the treatments with 1 and 2% of geraniol, and 2% of cuminaldehyde. Further, a reduction of chlorophyll contents and total phenolic compounds of barnyardgrass leaves was noticed, indicating that the monoterpenes cause adverse effect on photosynthesis and weed metabolism. Based on the results of this study, it can be concluded that the monoterpenes, particularly geraniol, (R)-carvone, and cuminaldehyde, can be used as potential natural herbicides.


Author(s):  
Tatyana Yoncheva ◽  
Attila Kantor ◽  
Eva Ivanišova ◽  
Nataliia Nikolaieva

A study of the chemical composition, the antioxidant capacity and the sensory characteristics of three Bulgarian wines (vintage 2015) from the native cultivars Misket Vrachanski (white), Pamid (red) and Gamza (red), grown in the region of Pleven, Central Northern Bulgaria was carried out. The results showed that the wine composition was mainly determined by the cultivar and its peculiarities. Misket Vrachanski white wine had the lowest rate of sugar-free extract, glycerol, total phenolic compounds and the highest acid content. From the red wine samples, Pamid had lower rates for the above parameters compared to Gamza. The experimental wines were analyzed for total content of esters, aldehydes and higher alcohols. A correlation was found between the rates of the studied volatile components in the wines and their aromatic sensory characteristics. At the wine tasting, Misket Vrachanski that contained the most of esters and aldehydes, was the best evaluated, followed by Gamza and Pamid. There was no correlation between the amount of total phenolic compounds in the experimental samples and their antioxidant capacity, which according to ABTS and MRAP methods increased in the order of Pamid < Misket Vrachanski < Gamza.


2020 ◽  
Vol 72 (2) ◽  
pp. 193-201
Author(s):  
Ahmet Emir ◽  
Ceren Emir

Many species of the Allium genus, principally the cultivated forms, are widely used as vegetables, spices and natural therapeutics due to their beneficial health properties. This study aimed to identify the phenolic composition and biological activities of the bulb, stem and flower parts of Allium pallens L., collected from two different localities. A total of 28 phenolic compounds were investigated by LC-ESI-MS/MS, and gallic acid, 4-hydroxybenzoic acid, and benzoic acid were found to be the major phenolic compounds in the plants from both locations. Total phenolic- and flavonoid-content analyses of samples were carried out using spectrophotometry, and the stem extracts were found to be rich in phenolics. DPPH, ABTS, FRAP and CUPRAC assays were used to determine the antioxidant capacities of the extracts. A linear relation was observed between the phenolic contents of the extracts and their antioxidant activities, and the stem extracts of plants from both locations were found to have potent antioxidant capacity. The inhibitory activities of the extracts against acetylcholinesterase, butyrylcholinesterase and tyrosinase were determined using a 96-well microplate reader. The antibutyrylcholinesterase activity of the extracts was found to be the highest. The outcomes of these investigations were further explored, and the underlying structure of multivariate data was revealed using principal component analysis. This study presents the distribution of chemical constituents and biological activities of the different parts of A. pallens, and also contributes to further investigations of Allium species.


2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.


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