ANTIOXIDANT ACTIVITY OF TRITERPENE GLYCOSIDE (CORTUSOSIDE A) FROM CORTUSA MATTHIOLI L. PLANT

The antioxidant activity of triterpene glycoside, first isolated from the aboveground part of the plant Cortusa matthioli L. and identified as β-D-xylopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranoside-(1→3)-13β,28-epoxyolean-30-al-3β,16α-diol (cortusoside A), is studied. Tests for the ability of cortusoside A to bind 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals did not reveal any activity of this compound. However, in experiments to study the ability to chelate Fe2+ ions, its sufficiently high iron chelating activity was found, which was only 2.24 times lower compared to the powerful Fe2+ chelator dipyridyl. The EC50 values for cortusoside A and dipyridyl were 0.417±0.057 and 0.186±0.018 mM, respectively. Literature analysis has shown that the structural analogue of cortusoside A, saxifragifolin B, has a much weaker iron chelating ability (13,4 times) compared to the standard Fe2+ EDTA-Na2 ion chelator, as well as a weak ability to bind free radicals of DPPH compared to the reference antioxidants – catechin and ascorbic acid (50 and 32 times, respectively). Despite the structural identity of the molecules cortusoside A and saxifragifolin B, low radiculopathy activity cortusosoide A may be due to differences in the structure of these substances (optical or geometric isomerism), as well as different methods were used in its definition.

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Abortifacient and Antioxidant Activities of Avicennia marina

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.33.12 ◽

2015 ◽

Vol 33 ◽

pp. 12-26

Author(s):

Muppaneni Srikanth ◽

Battu Ganga Rao ◽

Mallikarjuna Rao Talluri ◽

T. Rajananda Swamy

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Plant Extracts ◽

Antioxidant Activities ◽

Avicennia Marina ◽

Standard Drug ◽

Hydroalcoholic Extract ◽

Pure Compounds ◽

Post Implantation

The current study was intended to evaluate abortifacient and antioxidant activity of Avicennia marina leaves extracts. Abortifacient activity was evaluated in rats, compared with standard drug (Mifepristone) and antioxidant activity was evaluated by using three free radicals (Superoxide, Hydroxyl and DPPH) compared with Ascorbic acid. The extracts were showed pre implantation loss, post implantation loss of implantations and decreased the survival ration of foetuses. Among all extracts hydroalcoholic extract showed better activity. The selected plant extracts showed concentration dependent percentage inhibition of free radicals. Among three extracts hydroalcoholic extract showed better activity with IC50 values on superoxide, hydroxyl and DPPH radicals were 203μg, 237μg and 143μg. From the results obtained during the study it the hydroalcoholic extract was used for the isolation of pure compounds, and isolated the different compounds (β-sitosterol, Lupeol and Betulinic acid).

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Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

Photon: Jurnal Sain dan Kesehatan ◽

10.37859/jp.v11i2.2225 ◽

2021 ◽

Vol 11 (2) ◽

pp. 104-110

Author(s):

Alfin Surya ◽

Zaiyar Nazir ◽

Anggun Syazulfa

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Methanol Extract ◽

Natural Antioxidants ◽

Positive Control ◽

Phytochemical Screening ◽

Bioactive Components ◽

Ic50 Value ◽

Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

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Discovery of New N-hydrazinecarbothioamide Indazole Hybrids: As Potential Radical (ABTS and DPPH) Scavengers

Letters in Drug Design & Discovery ◽

10.2174/1570180817999200424074455 ◽

2020 ◽

Vol 17 (9) ◽

pp. 1177-1185

Author(s):

Rafaila Rafique ◽

Arshia ◽

Kanwal ◽

Khalid Mohammed Khan ◽

Sridevi Chigurupati ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavengers ◽

Radical Scavengers ◽

Synthetic Compounds ◽

Free Radical Generation ◽

Radical Generation

Background: Free radicals are the main cause of numerous diseases. Their overproduction needs to be controlled in order to combat several ailments. The current study deals with the discovery of new free radical scavengers. Methods: Substituted N-hydrazinecarbothioamide indazoles 1-18 were evaluated for DPPH and ABTS radical scavenging activities. Results: All synthetic compounds possess good radical DPPH and ABTS scavenging potential in the ranges of IC50 = 2.11 ± 0.17 - 5.3 ± 0.11 μM and IC50 = 2.31 ± 0.06 - 5.5 ± 0.07 μM, respectively, as compared to standard ascorbic acid having IC50 = 2.02 ± 0.11 μM for DPPH and IC50 = 2.1 ± 0.07 μM for ABTS. Conclusion: These compounds could serve as leads for antioxidant activity that have the ability to control free radical generation and ward off free radical-induced disorders.

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Antioxidant Potential of Epigallocatechin-3-gallate, Ascorbic Acid, and Sodium Ascorbate in Solution and Gel Forms by 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay

Conservative Dentistry Journal ◽

10.20473/cdj.v11i1.2021.19-23 ◽

2021 ◽

Vol 11 (1) ◽

pp. 19

Author(s):

Virdah Dwi Dewaantari ◽

Setyabudi Setyabudi ◽

Kun Ismiyatin

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Sodium Ascorbate ◽

Assay Method ◽

Dpph Assay ◽

Electron Pairs ◽

Healing Bone

Background: Free radicals are molecules without any electron pairs, unstable, and highly reactive. Antioxidants are needed to reduce free radicals. Antioxidants provide various benefits in dentistry as a preventive agent for caries, healing, bone formation, mouthwash, preventive and therapeutic cancer, and reduction of periodontal disease progressions. Additionally, research on antioxidants is still undergone due to the existence of free radical residues on bleached teeth. Epigallocatechin-3-gallate (EGCG), ascorbic acid (AA), and sodium ascorbate (SA) are ingridients that have antioxidant properties. Antioxidants can be in two forms solution and gel. Solutions have a higher substance releasing power than gel. Gel is extremely adhesive, so it does not flow easily. Meanwhile, solutions are rather unstable because it flows easily. Antioxidant activities were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay method. Purpose: Analyzing through literature reviews the potential antioxidants of EGCG, AA, and SA in solution and gel forms by DPPH Assay. Reviews: There were eight journal articles used in this review. The first article described antioxidant solutions in which EGCG was higher than sodium ascorbate. The second article showed that antioxidant gel EGCG was lower than sodium ascorbate. The third journal explained that ascorbic acid was higher than sodium ascorbate both in solution and gel forms. The other articles provided some information about the antioxidant activity percentages of EGCG, SA, and AA in the forms of gel and solutions by DPPH assay. Conclusion: EGCG has a higher antioxidant activity than SA, but it is lower when compared to AA in both solution and gel forms by DPPH assay.

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3,3’-di-O-methylellagic acid, an Antioxidant Phenolics Compound from Sonneratia alba Bark

Jurnal Natur Indonesia ◽

10.31258/jnat.15.1.63-67 ◽

2014 ◽

Vol 15 (1) ◽

pp. 63

Author(s):

Netti Herawati ◽

Firdaus Firdaus

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

2D Nmr ◽

Nmr Spectrometry ◽

Sonneratia Alba ◽

Antioxidant Agent ◽

Mangrove Trees

Free radicals play an important role in some pathogenesis of serious diseases, such as neurodegenerative disorders, cancer,liver cirrhosis, cardiovascular diseases, atherosclerosis, cataracts, diabetes and inflammation. Compounds that can scavengefree radicals have great potential in ameliorating these diseases. It is reported that phenolic compounds in plants possessstrong antioxidant activity and may help to protect cells against the oxidative damage caused by free radicals. Previousstudy revealed that mangrove trees, Sonneratia alba Bark showed strong antioxidant activity. Ethyl acetate fractionexhibited the best antioxidant performance. The antioxidant activity of this fraction was attributed to the presence ofcompounds of different polarities such as phenolics. Furthermore, the phenolic antioxidant of ethyl acetate fraction werepurified and identified with UV, IR, MS, and 2D NMR spectrometry. 3,3’-di-O-methylellagic acid was found in brownamourphous powder. Antioxidant activity was evaluated and compared with L-(+)-ascorbic acid as standard by usingDPPH assay. The compound has strong antioxidant activity than ascorbic acid standar with the value of IC 50 11.35 and17.64 μg/mL, respectively. The high value of antioxidant activity of compound indicate that S. alba is a potential source ofnatural antioxidant agent.

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Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00897 ◽

2021 ◽

pp. 5157-5162

Author(s):

Fatimah A. Jasim ◽

Hameed Salman Al-Hilu

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Aqueous Extract ◽

Allium Sativum ◽

Octadecadienoic Acid ◽

Octadecenoic Acid ◽

Organosulfur Compounds ◽

Mass Analysis ◽

Total Antioxidant

Garlic is one of many medicinal plants that used over thousands of years due to its organosulfur compounds. Materials and methods: commercial garlic was used for the extraction, phytochemicals detections and Gas Chromatography-Mass (GC- Mass) analysis utilized to determine the main compounds in the aqueous extract. Antioxidant activity was evaluated in the garlic extract using total antioxidant capacity (TAC) and DPPH for detecting the concentration that takes half of the free radicals. The phytochemical tests revealed several compounds in the extract, included alkaloid, glycosides, tannins, and flavonoid and phenol while saponin compound was not detected. GC-Mass spotted 15 different compounds included allyl trisulfide with the concentration (1.89%) followed by ascorbic acid 2, 6-dihexadecanoate (22.24%), 9, 12-Octadecadienoic acid (Z, Z) (11.44), and 6-Octadecenoic acid, (Z) (40, 28). Total Antioxidant Activity of garlic aqueous extract reached 60 mg/ml compared to that of ascorbic acid. IC50 examination observed that the aqueous extract of garlic had 308.57µg/ml value.

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EVALUACIÓN DE LA ACTIVIDAD ANTIOXIDANTE DEL NONI (Morinda citrifolia L.) EN TRES ESTADOS DE MADUREZ EN TINGO MARIA

Revista ECIPeru ◽

10.33017/reveciperu2009.0004/ ◽

2019 ◽

pp. 19-22

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

Natural Antioxidants ◽

Morinda Citrifolia ◽

Peruvian Amazon ◽

Total Polyphenols ◽

Maturity Stages ◽

Palabras Clave

EVALUACIÓN DE LA ACTIVIDAD ANTIOXIDANTE DEL NONI (Morinda citrifolia L.) EN TRES ESTADOS DE MADUREZ EN TINGO MARIA ANTIOXIDANT ACTIVITY EVALUATION OF NONI (Morinda citrifolia L.) FROM THREE MATURITY STAGES HARVESTED IN TINGO MARIA Joel P. Sullón Vargas, Elizabeth S. Ordóñez Gómez, Johan G. Vela Romero, Manuel Sandoval Chacón DOI: https://doi.org/10.33017/RevECIPeru2009.0004/ RESUMEN En la actualidad existe mucho interés por la búsqueda de antioxidantes de fuentes naturales incluyendo el noni (Morinda citrifolia L.). Su consumo es promocionado debido a sus propiedades funcionales que posee. El objetivo fue determinar el contenido de ácido ascórbico, polifenoles totales y actividad antioxidante del jugo de noni en tres estados de madurez: pintón, maduro y sobre maduro. Los antioxidantes analizados fueron: ácido ascórbico y polifenoles totales. La actividad antioxidante se midió utilizando los métodos: DPPH, ABTSº+ y Peroxilo. Los resultados indican que el fruto en estado maduro tuvo el mayor contenido de ácido ascórbico 253±1,9mgAA/100ml; y polifenoles totales 232±6,8 mg de catequina/100ml de jugo de noni. Para el radical DPPH se encontró como mejor tratamiento en el estado maduro IC50 149,5±0,6ug/ml; en el método del catión ABTSº+ se encontró que el estado de madurez pintón ejerció mayor efecto sobre la capacidad antioxidante IC50 145,5±0,6ug/ml y este mismo estado de madurez fue el mejor en el radical peroxilo IC50 106,1±2,0ug/ml. En consecuencia, el consumidor dispone de un fruto rico en antioxidantes, que podrían ayudar a aliviar problemas de salud asociados a estrés oxidativo y enfermedades crónicas. Palabras clave: Noni, Acido ascórbico, Polifenoles totales, Antioxidantes, Radicales libres. ABSTRACT There is a lot of interest to find antioxidants from natural resources, especially fruits that grow well in the Peruvian Amazon such as noni (Morinda citrifolia L.). This fruit is being used by the nutraceutical industry because of its functional properties. However, its antioxidant capacity has not being fully investigated. The purpose of this study was to determine the content of ascorbic acid, total polyphenols and antioxidant activity of noni juice under three maturity stages: unripe, ripe and over-ripe. Ascorbic acid and total polyphenols were determined by spectrophotometry. The antioxidant activity was quantified using the DPPH, ABTSº+ and peroxyl methods. Result from the ripe noni had the highest content of ascorbic acid 253±1,9mgAA/100ml) and total polyphenols 23±6,8mg de catechin/100ml. DPPH results showed that the best treatment was ripe noni IC50 149,5±0,6ug/ml. However, unripe noni provided best results for ABTSº+ (IC50 145,5±0,6ug/ml) and peroxyl inhibition (IC50 106,1±2,0ug/ml). Collectively, our results demonstrated that a juice prepared with unripe and ripe noni could significant amounts of natural antioxidants and help alleviate oxidative stress and chronic inflammation. Keywords: Noni, Ascorbic acid, Total polyphenols, Antioxidants, Free radicals.

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ANTIOXIDANT ACTIVITY POTENCY OF CHITOSAN FROM HARUAN (CHANNA STRIATA) SCALES

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v5i2.8951 ◽

2020 ◽

Vol 5 (2) ◽

pp. 139

Author(s):

Deby Kania Tri Putri ◽

Beta Widya Oktiani ◽

Candra Candra ◽

Rosihan Adhani

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radicals ◽

The Body ◽

Control Group ◽

Polymorphonuclear Cells ◽

Fish Scales ◽

Significant Difference ◽

Channa Striata ◽

Dpph Solution

ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.Keywords: Antioxidants, Chitosan, Haruan fish scalesABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity. Keywords: Antioxidants, Chitosan, Haruan fish scales.

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