Bioproducts against food-borne pathogenic bacteria

Advances in biotechnology research show the rising generation of a variety of products derived from microbial, plants and animal sources. These products are known as “bioproducts” or “natural products”. The preservation of the microbiological quality of foods without the use of chemical preservatives has become a challenge stimulating new researches on conservation alternatives. The aims of this study were the assessment of the the antibacterial activity of several bioactive compounds: essential oils of orange, lavender, green and red mandarins; ethanol extract of oregano and protein hydrolyzed from shrimp shell against Escherichia coli and Staphylococcus aureus isolated from foods, and the comparison between two different methods used in the screening of natural products with potential antibacterial activity. The antibacterial activities of the natural compounds were determined using agar diffusions tests and bioautography methods. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined for the essential oils of orange and lavender, and the ethanolic oregano extract. The essential oils of orange and lavender and the ethanolic oregano extract showed antimicrobial activity against all bacteria tested in the study. The agar disk diffusion and the bioautographic methods showed no significant difference in the evaluation of the biological activity of natural products.

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Chemical composition and antibacterial activities of the essential oils from Ocotea zahamenensis Van Der Werff (Lauraceae)

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2021.16.1.0202 ◽

2021 ◽

Vol 16 (1) ◽

pp. 115-125

Author(s):

Razafiarimanga Zara Nomentsoa ◽

Randriamampianina Lovarintsoa Judicael ◽

Randrianarivo Hanitra Ranjàna ◽

Ralitera Andrianirina Manampisoa ◽

Rakoto Danielle Aurore Doll ◽

...

Keyword(s):

Antibacterial Activity ◽

Body Weight ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Vibrio Fischeri ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Plant Parts ◽

Detection Analysis ◽

Toxicological Studies

The present work aimed to study the composition and antibacterial properties of the essential oils (EO) of Ocotea zahamenensis leaves (LEO), stem (SEO) and root (REO) barks from two harvest periods (March and June). All EOs were extracted by hydrodistillation from fresh plant parts with yields up to 4.5%. They are colourless, clear, with a strong odour, heavy, levogyre, with a low acid index and an ester index up to 14.89. Gas chromatography/flame ionisation detection analysis of these EOs identified 5 to 12 components representing 96.06 to 99.96% of the overall composition. Safrole was by far the most predominant constituent with contents ranging from 77.45% (SEO, June) to 97.05% (REO, March). The antibacterial activity was tested against eight pathogenic bacteria including 4 Gram (-) and 4 Gram (+) using microdilution assays. With Minimum Inhibitory Concentration (MIC) values of less than 1 mg/mL, all EOs showed antibacterial activity which varied according to the strain. There was not much difference between the activities of March and June Eos, and in both cases SEO were slightly more effective than LEO and REO. All EOs had bacteriostatic action on Bacillus cereus and Vibrio fischeri and bactericidal on almost other strains. When administered orally to mice at 0.5 mg/kg body weight, all EOs caused symptoms of intoxication. Their LD50 varied from 1.019 to 2.73 g/kg body weight. These EOs could be a new source of safrole and could be used for various purposes with further toxicological studies.

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Antibacterial Activity of Dacryodes edulis Seed Extracts on Food-Borne Pathogens

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v4i1.3 ◽

1970 ◽

Vol 4 (1) ◽

pp. 17-21 ◽

Cited By ~ 3

Author(s):

BA Omogbai ◽

TO Eneh

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Pharmaceutical Preparation ◽

Water Extract ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Food Borne ◽

Dacryodes Edulis ◽

Seed Extracts

The antibacterial effect of aqueous and ethanolic seed extracts of Dacryodes edulis was studied using agar diffusion technique. Extracts tested at a final concentration 2000;g/ml produced in vitro antibacterial activities in assays against food-borne bacterial isolates of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi and Proteus mirabilis. The highest zone of inhibition was obtained from ethanol extract at 2000;g/ml against Escherichia coli with a diameter of 28mm.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the water extract ranged between 62.5-500;g/ml and 125-1000;g/ml respectively. The MIC and MBC of the ethanol extract ranged between 31.25-250;g/ml and 62.5-500;g/ml. The percentage inhibition of the isolates at 1000;g/ml was more in the ethanol extract (65.4-95.5%) compared to the water extract (30-2-60.2%). This study shows that the extracts of Dacryodes edulis have potential usage as a food preservative and pharmaceutical preparation. Keywords: Dacryodes edulis, Antibacterial activity, Aqueous, Ethanolic, Seed extract

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Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms

Iranian Journal of Microbiology ◽

10.18502/ijm.v12i1.2517 ◽

2020 ◽

Author(s):

Mahmoud Osanloo ◽

Abbas Abdollahi ◽

Alireza Valizadeh ◽

Niloufar Abedinpour

Keyword(s):

Particle Size ◽

Antibacterial Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Activities ◽

Mentha Piperita ◽

Zataria Multiflora ◽

Microdilution Method ◽

Better Than ◽

Antibacterial Potential

Background and Objectives: Plant-derived essential oils (EOs) shave many usages in health and medicine, such as anti- bacterial agents. The aim of this study was the improvement of antibacterial activities of two EOs using nanotechnology. Materials and Methods: Antibacterial activity was investigated on four important human pathogenic bacteria using the 96-well plate microdilution method, a quantitative approach. Eleven formulations were prepared using each of the EOs. Eventually, the best nanoformulation with the smallest particle size and polydispersive indices (PDI and SPAN) was selected using each EO for further investigations. Moreover, two microemulsions with similar ingredients and the same portion in comparison with two selected nanoemulsions were also prepared. Antibacterial activity of each EO was compared with its micro- and nano-emulsions. Results: The antibacterial efficacy of Zataria multiflora EO (ZMEO) was significantly better than Mentha piperita EO (MPEO). Besides, the antibacterial activity of nanoemulsion of ZMEO with a particle size of 129 ± 12 nm was significantly better than no- and micro-formulated forms of ZMEO. Interestingly, the efficiency of MPEO nanoemulsion (160 ± 25 nm) was also significantly better than MPEO and its micro-formulated form. Conclusion: Regardless of the intrinsic antibacterial property of two examined EOs, by formulating to nanoemulsion, their efficiencies were improved. Nanoemulsion of ZMEO introduced as an inexpensive, potent and green antibacterial agent.

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Correlation between Chemical Composition of Greek Essential Oils and their Antibacterial Activity against Food-borne Pathogens

Natural Product Communications ◽

10.1177/1934578x0700200413 ◽

2007 ◽

Vol 2 (4) ◽

pp. 1934578X0700200 ◽

Cited By ~ 4

Author(s):

Nikos G. Chorianopoulos ◽

Epameinontas T. Evergetis ◽

Nektarios Aligiannis ◽

Sofia Mitakou ◽

George-John E. Nychas ◽

...

Keyword(s):

Gas Chromatography ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Salmonella Enteritidis ◽

Principal Component ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Food Borne

The compositions of forty-nine essential oils obtained from plants growing in areas of biodiversity in Greece were determined by means of gas chromatography and gas chromatography–mass spectrometry. The in vitro antibacterial activities of the oils were evaluated against a panel of five common food-borne bacteria ( Escherichia coli 0157:H7 NCTC 12900, Salmonella Enteritidis PT4, Staphylococcus aureus ATCC 6538, Listeria monocytogenes Scott A and Bacillus cereus FSS 134). Monoterpene hydrocarbons, phenolic monoterpenes and alcohols were the major constituents of the oils, but their concentrations varied greatly between the samples examined. The essential oils obtained from the species endemic to Greece ( Satureja and Origanum) had the most potent bactericidal properties. The chemical composition data were statistically processed through Principal Component Analysis (PCA) and correlated with the antibacterial activities, allowing a rough estimation of the potential of the essential oils antibacterial activity.

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Chemical Composition and Antibacterial Activity of Essential Oils ofTagetes minuta(Asteraceae) against Selected Plant Pathogenic Bacteria

International Journal of Microbiology ◽

10.1155/2016/7352509 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Martin Muthee Gakuubi ◽

John M. Wagacha ◽

Saifuddin F. Dossaji ◽

Wycliffe Wanzala

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Scientific Basis ◽

Diffusion Method ◽

Phytopathogenic Bacteria ◽

Xanthomonas Axonopodis

The objective of this study was to determine the chemical composition and antibacterial activity of essential oils (EOs) ofTagetes minutaagainst three phytopathogenic bacteriaPseudomonas savastanoipv.phaseolicola,Xanthomonas axonopodispv.phaseoli, andXanthomonas axonopodispv.manihotis. The essential oils were extracted using steam distillation method in a modified Clevenger-type apparatus while antibacterial activity of the EOs was evaluated by disc diffusion method. Gas chromatography coupled to mass spectrometry (GC/MS) was used for analysis of the chemical profile of the EOs. Twenty compounds corresponding to 96% of the total essential oils were identified with 70% and 30% of the identified components being monoterpenes and sesquiterpenes, respectively. The essential oils ofT. minutarevealed promising antibacterial activities against the test pathogens withPseudomonas savastanoipv.phaseolicolabeing the most susceptible with mean inhibition zone diameters of 41.83 and 44.83 mm after 24 and 48 hours, respectively.The minimum inhibitory concentrations and minimum bactericidal concentrations of the EOs on the test bacteria were in the ranges of 24–48 mg/mL and 95–190 mg/mL, respectively. These findings provide a scientific basis for the use ofT. minutaessential oils as a botanical pesticide for management of phytopathogenic bacteria.

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Effect of Harvest Period on Yield, Chemical Composition and Antibacterial Activity of the Essential Oils of Thymus Satureioides

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8033 ◽

2017 ◽

Vol 9 (1) ◽

Author(s):

Mekkaoui Salma ◽

Farah Abdellah ◽

Elkhanchoufi Abdesalam ◽

Elkhetabi Assia ◽

Moutaouafiq Salma ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Mycobacterium Smegmatis ◽

Aerial Part ◽

Pathogenic Bacteria ◽

Flowering Season ◽

Food Borne ◽

Thymus Satureioides

This study was performed to examine the yield, the chemical composition and the antibacterial activity of essential oils of thymus satureioides, grown in two different periods (flowering and after flowering) in the south of Morocco. These two essential oils are obtained by hydrodistillation along with using Clevenger and then they are tested on pathogenic bacteria: Escherichia coli, Mycobacterium smegmatis and Bacillus subtilis, responsible of food borne disease in the region of Fez. The extraction of the essential oil of Thymus satuerioides in the Laboratory of National Institute of Aromatic and Medicinal Plants (NIAMP), Taounate, Morocco for extraction has provided a yield important enough to 0.75 % in Period of flowering compared to gasoline extract of the aerial part after flowering season which is 0.62 %. The chromatographic analyzes and spectrometric have helped to determine the chemical composition of essential oils of approximately 23 constituents of these plants that are different by the harvest period for each one.

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Chemical and Antibacterial Activity Evaluation of Alpinia calcarata and Alpinia zerumbet Grown in Foothills Agroclimatic Conditions of Northern India

The Open Bioactive Compounds Journal ◽

10.2174/1874847302109010015 ◽

2021 ◽

Vol 9 (1) ◽

pp. 15-19

Author(s):

Gunjan Bhatt ◽

Ranveer S. Nagarkoti ◽

Sushma Kholiya ◽

Ameeta Tiwari ◽

Sajendra K. Verma ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Activities ◽

Good Activity ◽

Filter Paper Disc ◽

E Coli ◽

Northern India ◽

Leaf Oil ◽

Alpinia Zerumbet

Background: The member of the genus Alpinia (family: Zingiberaceae) is used in traditional medicine for various formulations for the food, spices, medicines, and perfume. Objective: The present study was carried out to analyse the compositional variability in leaf and rhizome essential oils of Alpinia calcarata Roscoe and Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and to evaluate their antimicrobial activity against eight pathogenic bacteria strains. Methods: The essential oils were extracted by hydrodistillation and analysed by GC and GC-MS and the antibacterial activity was evaluated by filter paper disc diffusion and micro dilution broth assay. Results: A total of 31 compounds identified, forming 89.26-94.32% composition of A. calcarata and A. zerumbet. The leaf and rhizome oil of A. calcarata and A. zerumbet were mainly characterized by 1,8-cineole (15.61-43.63%), β-pinene (5.02-23.52%), terpinen-4-ol (1.00-20.87%), camphor (1.94-11.60%), and (Z)-β-ocimene (0.16-11.86%). endo-Fenchyl acetate (13.12-24.39%) was identified as marker constituents of rhizome essential oil of both A. calcarata and A. zerumbet. The antibacterial assay showed that leaf oil of A. calcarata has good activity against S. mutans, whereas its rhizome oil possess good activity against K. pneumoniae, E. coli, S. aureus, and S. epidermidis. However, the rhizome oil of A. zerumbet showed activity against S. mutans, B. subtilisS. aureus-2940 and S. epidermidis. Conclusion: The essential oils of A. calcarata and A. zerumbet with aroma chemicals viz. 1,8-cineole, ocimenes, terpinen-4-ol, α-pinene, β-pinenes and fenchyl acetate and significant antibacterial activities could be used for perfumery and fragrance related formulation.

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Yield, Phytochemical Constituents, and Antibacterial Activity of Essential Oils from the Leaves/Twigs, Branches, Branch Wood, and Branch Bark of Sour Orange (Citrus aurantium L.)

Processes ◽

10.3390/pr7060363 ◽

2019 ◽

Vol 7 (6) ◽

pp. 363 ◽

Cited By ~ 20

Author(s):

Mohammad K. Okla ◽

Saud A. Alamri ◽

Mohamed Z.M. Salem ◽

Hayssam M. Ali ◽

Said I. Behiry ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Antibacterial Activities ◽

Sour Orange ◽

Citrus Aurantium ◽

Dickeya Solani ◽

First Time ◽

Branch Wood

In the present work, essential oils (EOs) extracted from different parts of sour orange Citrus aurantium (green leaves/twigs, small branches, wooden branches, and branch bark) were studied through gas chromatography coupled with mass spectrometry (GC/MS). Furthermore, the EOs in the amounts of 5, 10, 15, 20, and 25 µL were studied for their antibacterial activity against three pathogenic bacteria, Agrobacterium tumefaciens, Dickeya solani, and Erwinia amylovora. The main EO compounds in the leaves/twigs were 4-terpineol (22.59%), D-limonene (16.67%), 4-carvomenthenol (12.84%), and linalool (7.82%). In small green branches, they were D-limonene (71.57%), dodecane (4.80%), oleic acid (2.72%), and trans-palmitoleic acid (2.62%), while in branch bark were D-limonene (54.61%), γ-terpinene (6.68%), dodecane (5.73%), and dimethyl anthranilate (3.13%), and in branch wood were D-limonene (38.13%), dimethyl anthranilate (8.13%), (-)-β-fenchol (6.83%), and dodecane (5.31%). At 25 µL, the EO from branches showed the highest activity against A. tumefaciens (IZ value of 17.66 mm), and leaves/twigs EO against D. solani and E. amylovora had an IZ value of 17.33 mm. It could be concluded for the first time that the wood and branch bark of C. aurantium are a source of phytochemicals, with D-limonene being the predominant compound in the EO, with potential antibacterial activities. The compounds identified in all the studied parts might be appropriate for many applications, such as antimicrobial agents, cosmetics, and pharmaceuticals.

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Antibacterial activity of desiccated cyanobacterium Anabaena sp. isolated from terracotta monuments of Bishnupur, West Bengal

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120201 ◽

2020 ◽

pp. 94-98

Author(s):

Shailen Bhakat ◽

Subarna Saha ◽

Sikha Mandal ◽

Jnanendra Rath

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Salmonella Typhimurium ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Microtiter Plates ◽

High Solar Radiation ◽

Anabaena Sp

Anabaena sp. are the dominant cyanobacterial species on terracotta monuments of Bishnupur which exposed to high solar radiation, ultraviolet and in a desiccated condition in most part of the year. In the present study three Anabaena species were isolated from crust samples and its antibacterial activities were evaluated against pathogenic bacteria Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, Escherichia coli. We observed good antibacterial activity in ethyl acetate and ethanol extract of Anabaena sp. (VBCCA 052002) which having highest antibacterial activity against Staphylococcus aureus, Salmonella typhimurium, Staphylococcus aureus and E. coli respectively. We have validated the antibacterial assay by using resazurin based antimicrobial assay in microtiter plates and calculated the MIC value of ethyl acetate extract of Anabaena sp. (VBCCA 052002) which is found to be 100 µg/ml against Staphylococcus aureus and 150 µg/ml against Salmonella typhimurium.

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Antibacterial Activity 96% Ethanol Extract of Brown Seaweed (Padina australis) from Poteran Island Madura against Staphylococcus aureus ATCC 25923

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2209 ◽

2021 ◽

Vol 14 (02) ◽

pp. 1059-1064

Author(s):

Nani Wijayanti DN ◽

Giftania Wardani Sudjarwo ◽

Oki Nugraha Putra

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Health Sector ◽

Brown Seaweed ◽

Ethanol Extract ◽

Diffusion Method ◽

Activity Test ◽

Significant Difference ◽

Macro Algae

Brown seaweed is one of the natural resources of the sea which is very abundant and grows naturally on the coast of Indonesia, especially in Madura. Lately, disease due to infection is one of the problems in the health sector that continues to grow. Some microorganisms that cause infections include bacteria, viruses, fungi and protozoa. One of the bacteria that can cause infection is Staphylococcus aureus. Staphylococcus aureus is one of pathogenic bacteria that cause abnormalities and infections to skin. The more infections cause an increase in the use of antibiotics, the greater the risk of increasing the incidence of resistance. This encourages the research of sources for antibacterial medicines from natural materials, namely macro algae. One of the macro algae that have antibacterial activity is brown seaweed from Padina australis species. This study uses laboratory experimental methods aimed at proving the antibacterial activity of 96% ethanol extract of brown seaweed Padina australis from the Madura Islands against Staphylococcus aureus ATCC 25923. The antibacterial activity test was carried out by the well diffusion method. The results of the antibacterial activity test showed that ethanol extract of 96% brown seaweed Padina australis with concentrations of 20%, 15%, 10%, and 5% had antibacterial activity against Staphylococcus aureus ATCC 25923 in the medium to strong category. Statistical test results (Kruskal Wallis) showed that there was a significant difference between the concentration and the inhibited zone produced (p = 0.037).

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