scholarly journals EXPERIMENTAL SUBSTANTIATION OF APPLICATION OZONIZED PHYSIOLOGICAL SOLUTION IN SURGICAL TREATMENT OF CHRONIC OSTEOMYELITIS

2016 ◽  
Vol 19 (3) ◽  
pp. 8-11
Author(s):  
I A Dotsenko ◽  
A K Chertkov ◽  
S N Skornyakov ◽  
B I Novikov ◽  
L A Golubeva
Keyword(s):  
Test Object ◽  
Culture Medium ◽  
High Efficiency ◽  
Saline Solution ◽  
Experimental Studies ◽  
The Body ◽  
Ozone Treatment ◽  
Experimental Conditions ◽  
Hematogenous Osteomyelitis ◽  
The Impact

Acute hematogenous osteomyelitis with inadequate treatment or no as such can become chronic. The latter involves a problem of not only increasing resistance of microorganisms to antibiotics (ABP), but their stable persistence in the body for a long time. Inability to achieve bactericidal concentrations of antibacterial drugs in the sequester due to lack of blood circulation in pyogenic foci makes inefficient traditional antimicrobial therapy. The purpose of this study - to justify the use of ozonated saline solution in the complex treatment of HGO through experimental research and development of optimal methods of influence on gram (+) and Gram (-) bacteria, fungi of the genus of Candida, Mycobacterium tuberculosis. We have carried out two series of experiments. To approximate the experimental conditions to the in vivo conditions of the surgical wound bacterial culture medium used - mylar fabric is hygroscopic and can largely mitigate the impact of aggressive antiseptic solutions to the CFU, simulating wall abscess (hereinafter referred to as the test object). One of a series of tubes with different concentrations of bacteria were exposed to ozonated saline with different exposures. The presence of colony growth test - bacteria on the test -object on the surface of the culture medium showed that ozone treatment for a given exposure time exposure did not provide a reliable germicidal effect. Lack of growth of colonies test - bacteria on test - and the object in a nutrient medium surface indicates the presence of bactericidal activity. The results of experimental studies have shown the high efficiency of ozonated saline against S. aureus ATCC 25923 with exposure 20 min., Ps. aeruginosa ATCC 27853 at 30 min exposure. In C. albicans strain 401 / 885-653 treatment ozonated saline solution has a bacteriostatic and pronounced inhibitory effect upon exposure of 30 minutes. This allows us to draw a conclusion on the appropriateness and effectiveness of the use of ozonated saline solution in the treatment of chronic hematogenous osteomyelitis of the spine.

scholarly journals The Impact of Time of Day on Energy Expenditure: Implications for Long-Term Energy Balance

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2383 ◽  
Author(s):  
Shaw ◽  
Leung ◽  
Jong ◽  
Coates ◽  
Davis ◽  
...  
Keyword(s):  
Energy Balance ◽  
Energy Expenditure ◽  
Energy Intake ◽  
Experimental Studies ◽  
Time Of Day ◽  
Circadian System ◽  
The Body ◽  
Energy Regulation ◽  
Meal Timing ◽  
The Impact

There is evidence to indicate that the central biological clock (i.e., our endogenous circadian system) plays a role in physiological processes in the body that impact energy regulation and metabolism. Cross-sectional data suggest that energy consumption later in the day and during the night is associated with weight gain. These findings have led to speculation that when, as well as what, we eat may be important for maintaining energy balance. Emerging literature suggests that prioritising energy intake to earlier during the day may help with body weight maintenance. Evidence from tightly controlled acute experimental studies indicates a disparity in the body’s ability to utilise (expend) energy equally across the day and night. Energy expenditure both at rest (resting metabolic rate) and after eating (thermic effect of food) is typically more efficient earlier during the day. In this review, we discuss the key evidence for a circadian pattern in energy utilisation and balance, which depends on meal timing. Whilst there is limited evidence that simply prioritising energy intake to earlier in the day is an effective strategy for weight loss, we highlight the potential benefits of considering the role of meal timing for improving metabolic health and energy balance. This review demonstrates that to advance our understanding of the contribution of the endogenous circadian system toward energy balance, targeted studies that utilise appropriate methodologies are required that focus on meal timing and frequency.

scholarly journals Integrated Bioinformatics Data Analysis and Experimental Studies Reveals Prognostic Significance of ALDH Family in Endometria Cancer

2021 ◽  
Author(s):  
Zhou-Tong Dai ◽  
Yuan Xiang ◽  
Xing-Hua Liao
Keyword(s):  
Cell Lines ◽  
Cox Regression ◽  
Prognostic Significance ◽  
Experimental Studies ◽  
Female Reproductive Tract ◽  
The Body ◽  
Mouse Tumor ◽  
The Impact

Abstract Background Uterine Corpus Endometrial Cancer (UCEC) is one of the three common malignant tumors of the female reproductive tract. According to reports, the cure rate of early UCEC can reach 95%. Therefore, the development of prognostic markers will help UCEC patients to find the disease earlier and develop treatment earlier. The ALDH family was first discovered to be the essential gene of the ethanol metabolism pathway in the body. Recent studies have shown that ALDH can participate in the regulation of cancer. Methods We used the gene profile data of 33 cancers in the TCGA database to analyze the expression and survival of the ALDH family. GO, KEGG, PPI multiple functional analysis was used to predict the regulatory role of ALDH family in cancer. In addition, using CCK-8, colony formation, nude mouse tumor formation and other methods, the in vitro function of UCEC cancer cell lines was tested to further confirm the key role of ALDH2 expression in the proliferation of UCEC cell lines. Finally, Lasso and Cox regression methods were used to establish an overall survival prognosis model based on ALDH2 expression. Result In our research, we explored the expression of ALDH family in 33 cancers. It was found that ALDH2 was abnormally expressed in UCEC. Besides, in vivo and in vitro experiments were conducted to explore the effect of ALDH2 expression on the proliferation of UCEC cell lines. Meanwhile, the change of its expression is not due to gene mutations, but is regulated by miR-135-3p. At the same time, the impact of ALDH2 changes on the survival of UCEC patients is deeply discussed. Finally, a nomogram for predicting survival was constructed, with a C-index of 0.798 and AUC of 0.764. Conclusion This study suggests that ALDH2 may play a crucial role in UCEC progression and has the potential as a prognostic biomarker of UCEC.

Clinical and experimental studies of immunо-infl ammatory mechanisms of anthracosilicosis formation

2020 ◽  
pp. 364-370
Author(s):  
N. I. Panev ◽  
A. S. Kazitskaya ◽  
O. Yu. Korotenko ◽  
G. A. Gerasimova ◽  
O. A. Morozova ◽  
...  
Keyword(s):  
Immune Response ◽  
Respiratory Failure ◽  
Acute Phase ◽  
Humoral Immunity ◽  
Experimental Studies ◽  
Early Period ◽  
The Body ◽  
Protective Mechanism ◽  
Experimental Conditions ◽  
Local Immunity

Introduction. In the structure of occupational diseases of employees of the main professions of the coal industry, respiratory diseases are widespread, in the process of formation of which the key role belongs to the immune system of the body. Early manifestations of the development of professional pathology, as a rule, remain unnoticed, and therefore there is a need to study the pathogenetic mechanisms underlying its formation, not only in clinical, but also in experimental conditions, allowing to assess the premorbid state of the body for timely diagnosis and treatment and prevention.The aim of the study is to study the immune-infl ammatory mechanisms of anthracosilicosis formation on the basis of clinical and experimental studies.Materials and methods. We examined 204 miners working in underground conditions with a signifi cant dustiness of workplaces exceeding the maximum permissible concentration by 10 or more times. Th e main group consisted of 115 workers with a previously established diagnosis of “anthracosilicosis”. Th e comparison group was formed from 89 miners without a diagnosis of respiratory pathology, working in similar sanitary conditions. To assess the dynamics of immuno-infl ammatory mechanisms in the experiment, modeling of dust pathology of the lungs was performed on 310 white male laboratory rats (220-experimental and 90 — control).Results. In patients with anthracosilicosis, the development of immune failure of humoral immunity mechanisms was revealed, which was manifested by a signifi cant decrease in the level of serum IgG against the background of an increase in the absolute and relative number of B-lymphocytes. Th e formation of anthracosilicosis is characterized by the active development of the immuno-infl ammatory process (an increase in the level of pro-infl ammatory cytokines and proteins of the acute phase of infl ammation), the severity of which increases when the disease is complicated by respiratory failure. Activation of the synthesis of anti-infl ammatory IL-4, which is a powerful inhibitor of macrophage infl ammation and slows down the processes of fi brosis in the bronchopulmonary system, acts as a protective mechanism that prevents the formation of respiratory failure in miners with anthracosilicosis. Experimental modeling of anthracosilicosis revealed phase changes in the immune response. In the early period of exposure to dust factor was observed activation of humoral (increased level of all classes of immunoglobulins) and the subsequent development of the inflammatory process (increased concentrations of acute phase proteins of inflammation) in the background of the balance between subpopulations of T-lymphocytes to ensure proper development of protective immune response. Long-term intake of antigen was characterized by violations of humoral immunity, the predominance of cell-type reactions and the chronization of the infl ammatory process.Conclusions. Th e study of immuno-infl ammatory mechanisms of anthracosilicosis formation in clinical and experimental conditions indicates the activation of urgent adaptation and maintenance of compensatory and adaptive reactions of the body in the early period of contact with dust antigen. Th e chronic form of anthracosilicosis is characterized by an imbalance of regulatory mechanisms, ineffi  ciency of local immunity and the intensive development of generalized immune infl ammation, which increases with the addition of infection and complication of respiratory failure.

scholarly journals Effects of oxidative stress during human and animal reproductions: A review

2015 ◽  
Vol 08 (01) ◽  
pp. 006-011
Author(s):  
Carolina B. Berchieri-Ronchi ◽  
Paula T. Presti ◽  
Ana Lucia A. Ferreira ◽  
Camila R. Correa ◽  
Daisy Maria F. Salvadori ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Deoxyribonucleic Acid ◽  
Experimental Studies ◽  
The Body ◽  
Defense Strategies ◽  
Reproduction Process ◽  
Reproduction Period ◽  
Cellular Components ◽  
The Impact

ABSTRACTGiven its high ability to damage important cellular components (lipids, proteins and deoxyribonucleic acid), oxidative stress is now recognized as one of the most common mechanisms associated with development of a variety of diseases and natural events such as pregnancy. During reproduction period, there is a change in the pro-oxidant and antioxidant balance due to the body and circulation modifications that are inherent to the pregnancy process. The present paper discusses the role of oxidative stress on the reproduction process. More effective defense strategies are needed to decrease the deleterious effects of oxidative-stress-induced gestation. This approach could be achieved by antioxidant status alteration. Further clinical and experimental studies are needed for better understanding of oxidative stress mechanism and the impact of antioxidant supplementation on reproduction.

scholarly journals Regulative Mechanism of Guanidinoacetic Acid on Skeletal Muscle Development and Its Application Prospects in Animal Husbandry: A Review

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhaoming Yan ◽  
Zhaoyue Yan ◽  
Shuangli Liu ◽  
Yunju Yin ◽  
Tai Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Muscle Development ◽  
Muscle Growth ◽  
Experimental Studies ◽  
Chemical Properties ◽  
Animal Husbandry ◽  
Nutritional Supplement ◽  
The Body ◽  
Guanidinoacetic Acid ◽  
The Impact

Guanidinoacetic acid is the direct precursor of creatine and its phosphorylated derivative phosphocreatine in the body. It is a safe nutritional supplement that can be used to promote muscle growth and development. Improving the growth performance of livestock and poultry and meat quality is the eternal goal of the animal husbandry, and it is also the common demand of today's society and consumers. A large number of experimental studies have shown that guanidinoacetic acid could improve the growth performance of animals, promote muscle development and improve the health of animals. However, the mechanism of how it affects muscle development needs to be further elucidated. This article discusses the physical and chemical properties of guanidinoacetic acid and its synthesis pathway, explores its mechanism of how it promotes muscle development and growth, and also classifies and summarizes the impact of its application in animal husbandry, providing a scientific basis for this application. In addition, this article also proposes future directions for the development of this substance.

scholarly journals Applications of ceramics in composite protective structures

2021 ◽  
Vol 2 (396) ◽  
pp. 52-66
Author(s):  
A. Dulnev ◽  
Keyword(s):  
High Speed ◽  
Analytical Approach ◽  
High Efficiency ◽  
Experimental Studies ◽  
Ceramic Materials ◽  
Ship Structures ◽  
Collapse Simulation ◽  
Crystalline Ceramic ◽  
Protective Structures ◽  
The Impact

Object and purpose of research. The object of the study is ceramic-containing protective structures. The purpose of the study is to substantiate the feasibility and effectiveness of using ceramics as part of standard ship structures for protection against the effects of high-speed damaging elements. Materials and methods. Composites of the "ceramic + steel", "ceramic + fiberglass" type, made with the use of poly-crystalline ceramic materials based on boron carbide, nitride and silicon carbide, aluminum oxide and some other types, are considered. The study is based on an analytical approach, the results of ballistic tests of ceramic-containing composites and computational collapse simulation of ceramics in the composition of armor structures. Main results. The results of experimental studies demonstrating the level of efficiency of ceramic-containing structures are presented. The influence of the fragmentation features of various types of ceramics on the ballistic robustness of protective structures is shown. Structural and technological solutions aimed at increasing the survivability and ensuring the mounting of ceramic-containing structures on the ship are presented. Conclusion. The high efficiency of ceramics use in ship structures for protection against the impact of high-speed damaging elements is demonstrated. The use of ceramics in protective structures allows to get a significant increase in the ballistic robustness of structures while ensuring their ballistic survivability.

scholarly journals On the Effect of Cavity Formation during the Water Entry of Flexible Bodies

2018 ◽  
Author(s):  
Riccardo Panciroli ◽  
Tiziano Pagliaroli ◽  
Giangiacomo Minak
Keyword(s):  
Fluid Motion ◽  
Free Fall ◽  
Time Frame ◽  
Water Entry ◽  
The Body ◽  
Structural Deformation ◽  
Cavity Formation ◽  
Experimental Conditions ◽  
Flexible Bodies ◽  
The Impact

Elastic bodies entering the water might experience Fluid-Structure Interaction phenomena introduced by the mutual interaction between the structural deformation and the fluid motion. Cavity formation, often misleadingly named cavitation, is one of these. This work presents the results of an experimental investigation on the water entry of deformable wedges impacting a quiescent water surface with pure vertical velocity in free fall. The experimental campaign is conducted on flexible wedges parametrically varying the flexural stiffness, deadrise angle, and drop height. It is found that under given experimental conditions cavity pockets forms beneath the wedge. Their generation mechanism is found to be ruled by a differential between structural and fluid velocities, which is introduced by the structural vibrations. Results show that the impact force during water entry of stiff bodies is always opposing gravity, while in case of flexible bodies might temporarily reverse its direction, with the body that is being sucked into the water within the time frame between the cavity formation and its collapse. Severe impacts might also generate a series of cavity generation and collapses.

scholarly journals On Air-Cavity Formation during Water Entry of Flexible Wedges

2018 ◽  
Vol 6 (4) ◽  
pp. 155 ◽  
Author(s):  
Riccardo Panciroli ◽  
Tiziano Pagliaroli ◽  
Giangiacomo Minak
Keyword(s):  
Fluid Motion ◽  
Free Fall ◽  
Time Frame ◽  
Water Entry ◽  
The Body ◽  
Structural Deformation ◽  
Cavity Formation ◽  
Experimental Conditions ◽  
Fluid Velocities ◽  
The Impact

Elastic bodies entering water might experience fluid–structure interaction phenomena introduced by the mutual interaction between structural deformation and fluid motion. Cavity formation, often misleadingly named cavitation, is one of these. This work presents the results of an experimental investigation on the water entry of deformable wedges impacting a quiescent water surface with pure vertical velocity in free fall. The experimental campaign is conducted on flexible wedges parametrically varying the flexural stiffness, deadrise angle, and drop height. It is found that, under given experimental conditions, cavity pockets form beneath the wedge. Their generation mechanism might be ascribed to a differential between structural and fluid velocities, which is introduced by structural vibrations. Results show that the impact force during water entry of stiff wedges are always opposing gravity, while, in case flexible wedges temporarily reverse their direction, with the body that is being sucked into the water within the time frame between the cavity formation and its collapse. Severe impact might also generate a series of cavity generation and collapses.

scholarly journals The assessment of the impact of the chosen exploational conditions of hydraulic arrangement on the working liquid condition

2018 ◽  
Vol 182 ◽  
pp. 01026 ◽  
Author(s):  
Joanna Wilczarska ◽  
Ewa Kuliś ◽  
Marcin Łukasiewicz ◽  
Łukasz Fornal ◽  
Natalia Dluhunovych
Keyword(s):  
High Efficiency ◽  
Experimental Studies ◽  
Working Fluid ◽  
Hydraulic Systems ◽  
Research Activities ◽  
Filtration System ◽  
Analysis Of Results ◽  
Liquid Condition ◽  
The Impact ◽  
Working Liquid

Hydraulic systems in modern vehicles and agricultural machines have many components. In order to achieve high efficiency of the system, the system requires an accurate filtration system that allows you to reduce the amount of contaminants. Therefore, an important aspect is to determine the content of impurities in the working liquid, so we can diagnose and then predict the durability of the entire hydraulic system. This paper presents selected methods and means of state assessment, as well as the methodology for testing the working fluid. The results of experimental studies conducted in accordance with the developed methodology have been presented, which made it possible to obtain an appropriate analysis of results and draw appropriate conclusions. The results of the research confirmed the validity of the research activities undertaken using the passive experiment.

scholarly journals Radon Over Kimberlite Pipes: Estimation of the Emanation Properties of Rocks (Lomonosov Diamond Deposit, NW Russia)

2021 ◽  
Vol 11 (13) ◽  
pp. 6065
Author(s):  
Evgeny Yakovlev ◽  
Andrey Puchkov
Keyword(s):  
Experimental Studies ◽  
State Level ◽  
The Body ◽  
Free State ◽  
Physical Factors ◽  
Diamond Deposit ◽  
Factors Affecting ◽  
Emanation Coefficient ◽  
Host Rocks ◽  
The Impact

In this paper, using the example of the Lomonosov diamond deposit, experimental studies of rocks were carried out to assess the main radiation and physical factors affecting the formation of the radon field over the kimberlite pipes of the Arkhangelsk diamondiferous province. For various types of rocks, represented by vent kimberlites, tuffaceous-sedimentary rocks of the crater and enclosing and overlying sediments, the following were studied: porosity, density, activity of radium-226, activity of radon in a free state, level of radon production, and emanation coefficient. The research results showed that the greatest amount of radon in a free state is produced by rocks of the near-pipe space, represented by the enclosing Vendian V2 deposits and characterized by high values of the emanation coefficient, radium activity, radon production level and porosity. This fact is associated with the structural and geological features of the near-pipe space, which was exposed to the impact of kimberlite magma on the host rocks. The lowest values of these parameters are characteristic of the kimberlites of the vent facies, which limits the formation of free radon in the body of the pipe. The results of the experimental studies create prospects for the development of emanation methods for searching for kimberlite pipes in the conditions of the Arkhangelsk diamondiferous province.

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