scholarly journals Regulative Mechanism of Guanidinoacetic Acid on Skeletal Muscle Development and Its Application Prospects in Animal Husbandry: A Review

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhaoming Yan ◽  
Zhaoyue Yan ◽  
Shuangli Liu ◽  
Yunju Yin ◽  
Tai Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Muscle Development ◽  
Muscle Growth ◽  
Experimental Studies ◽  
Chemical Properties ◽  
Animal Husbandry ◽  
Nutritional Supplement ◽  
The Body ◽  
Guanidinoacetic Acid ◽  
The Impact

Guanidinoacetic acid is the direct precursor of creatine and its phosphorylated derivative phosphocreatine in the body. It is a safe nutritional supplement that can be used to promote muscle growth and development. Improving the growth performance of livestock and poultry and meat quality is the eternal goal of the animal husbandry, and it is also the common demand of today's society and consumers. A large number of experimental studies have shown that guanidinoacetic acid could improve the growth performance of animals, promote muscle development and improve the health of animals. However, the mechanism of how it affects muscle development needs to be further elucidated. This article discusses the physical and chemical properties of guanidinoacetic acid and its synthesis pathway, explores its mechanism of how it promotes muscle development and growth, and also classifies and summarizes the impact of its application in animal husbandry, providing a scientific basis for this application. In addition, this article also proposes future directions for the development of this substance.

scholarly journals Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Katharina Metzger ◽  
Dirk Dannenberger ◽  
Armin Tuchscherer ◽  
Siriluck Ponsuksili ◽  
Claudia Kalbe
Keyword(s):  
Muscle Development ◽  
Muscle Growth ◽  
Extreme Temperature ◽  
Growth Inhibitor ◽  
Muscle Cells ◽  
The Body ◽  
Farm Animals ◽  
Neonatal Piglets ◽  
Protein Levels

Abstract Background Climate change and the associated risk for the occurrence of extreme temperature events or permanent changes in ambient temperature are important in the husbandry of farm animals. The aim of our study was to investigate the effects of permanent cultivation temperatures below (35 °C) and above (39 °C, 41 °C) the standard cultivation temperature (37 °C) on porcine muscle development. Therefore, we used our porcine primary muscle cell culture derived from satellite cells as an in vitro model. Neonatal piglets have limited thermoregulatory stability, and several days after birth are required to maintain their body temperature. To consider this developmental step, we used myoblasts originating from thermolabile (five days of age) and thermostable piglets (twenty days of age). Results The efficiency of myoblast proliferation using real-time monitoring via electrical impedance was comparable at all temperatures with no difference in the cell index, slope or doubling time. Both temperatures of 37 °C and 39 °C led to similar biochemical growth properties and cell viability. Only differences in the mRNA expression of myogenesis-associated genes were found at 39 °C compared to 37 °C with less MYF5, MYOD and MSTN and more MYH3 mRNA. Myoblasts grown at 35 °C are smaller, exhibit higher DNA synthesis and express higher amounts of the satellite cell marker PAX7, muscle growth inhibitor MSTN and metabolic coactivator PPARGC1A. Only permanent cultivation at 41 °C resulted in higher HSP expression at the mRNA and protein levels. Interactions between the temperature and donor age showed that MYOD, MYOG, MYH3 and SMPX mRNAs were temperature-dependently expressed in myoblasts of thermolabile but not thermostable piglets. Conclusions We conclude that 37 °C to 39 °C is the best physiological temperature range for adequate porcine myoblast development. Corresponding to the body temperatures of piglets, it is therefore possible to culture primary muscle cells at 39 °C. Only the highest temperature of 41 °C acts as a thermal stressor for myoblasts with increased HSP expression, but it also accelerates myogenic development. Cultivation at 35 °C, however, leads to less differentiated myoblasts with distinct thermogenetic activity. The adaptive behavior of derived primary muscle cells to different cultivation temperatures seems to be determined by the thermoregulatory stability of the donor piglets.

scholarly journals The Impact of Time of Day on Energy Expenditure: Implications for Long-Term Energy Balance

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2383 ◽  
Author(s):  
Shaw ◽  
Leung ◽  
Jong ◽  
Coates ◽  
Davis ◽  
...  
Keyword(s):  
Energy Balance ◽  
Energy Expenditure ◽  
Energy Intake ◽  
Experimental Studies ◽  
Time Of Day ◽  
Circadian System ◽  
The Body ◽  
Energy Regulation ◽  
Meal Timing ◽  
The Impact

There is evidence to indicate that the central biological clock (i.e., our endogenous circadian system) plays a role in physiological processes in the body that impact energy regulation and metabolism. Cross-sectional data suggest that energy consumption later in the day and during the night is associated with weight gain. These findings have led to speculation that when, as well as what, we eat may be important for maintaining energy balance. Emerging literature suggests that prioritising energy intake to earlier during the day may help with body weight maintenance. Evidence from tightly controlled acute experimental studies indicates a disparity in the body’s ability to utilise (expend) energy equally across the day and night. Energy expenditure both at rest (resting metabolic rate) and after eating (thermic effect of food) is typically more efficient earlier during the day. In this review, we discuss the key evidence for a circadian pattern in energy utilisation and balance, which depends on meal timing. Whilst there is limited evidence that simply prioritising energy intake to earlier in the day is an effective strategy for weight loss, we highlight the potential benefits of considering the role of meal timing for improving metabolic health and energy balance. This review demonstrates that to advance our understanding of the contribution of the endogenous circadian system toward energy balance, targeted studies that utilise appropriate methodologies are required that focus on meal timing and frequency.

scholarly journals Integrated Bioinformatics Data Analysis and Experimental Studies Reveals Prognostic Significance of ALDH Family in Endometria Cancer

2021 ◽  
Author(s):  
Zhou-Tong Dai ◽  
Yuan Xiang ◽  
Xing-Hua Liao
Keyword(s):  
Cell Lines ◽  
Cox Regression ◽  
Prognostic Significance ◽  
Experimental Studies ◽  
Female Reproductive Tract ◽  
The Body ◽  
Mouse Tumor ◽  
The Impact

Abstract Background Uterine Corpus Endometrial Cancer (UCEC) is one of the three common malignant tumors of the female reproductive tract. According to reports, the cure rate of early UCEC can reach 95%. Therefore, the development of prognostic markers will help UCEC patients to find the disease earlier and develop treatment earlier. The ALDH family was first discovered to be the essential gene of the ethanol metabolism pathway in the body. Recent studies have shown that ALDH can participate in the regulation of cancer. Methods We used the gene profile data of 33 cancers in the TCGA database to analyze the expression and survival of the ALDH family. GO, KEGG, PPI multiple functional analysis was used to predict the regulatory role of ALDH family in cancer. In addition, using CCK-8, colony formation, nude mouse tumor formation and other methods, the in vitro function of UCEC cancer cell lines was tested to further confirm the key role of ALDH2 expression in the proliferation of UCEC cell lines. Finally, Lasso and Cox regression methods were used to establish an overall survival prognosis model based on ALDH2 expression. Result In our research, we explored the expression of ALDH family in 33 cancers. It was found that ALDH2 was abnormally expressed in UCEC. Besides, in vivo and in vitro experiments were conducted to explore the effect of ALDH2 expression on the proliferation of UCEC cell lines. Meanwhile, the change of its expression is not due to gene mutations, but is regulated by miR-135-3p. At the same time, the impact of ALDH2 changes on the survival of UCEC patients is deeply discussed. Finally, a nomogram for predicting survival was constructed, with a C-index of 0.798 and AUC of 0.764. Conclusion This study suggests that ALDH2 may play a crucial role in UCEC progression and has the potential as a prognostic biomarker of UCEC.

scholarly journals Impact of Protein Diet Supplements on Athletes

2021 ◽  
Vol 6 (2) ◽  
pp. 1-3
Author(s):  
Mohammad Athar
Keyword(s):  
Muscle Growth ◽  
The Body ◽  
Protein Diet ◽  
Negative Effects ◽  
Protein Supplements ◽  
Diet Supplements ◽  
Negative Effect ◽  
The Impact ◽  
Protein Diets ◽  
Day By Day

Protein supplements are one of the most commonly consumed nutritional supplements, whether by professional athletes or amateurs, even by those who use them for aesthetic purposes instead of sporting ones. The thirst for becoming better is increasing day by day and so is the quest for competition. Sports have been one of the greatest of platforms to showcase one’s talent, thus leading to constant hustle for getting better day by day. To improve the performance, diet and physical appearance of the body i.e. muscle growth of athletes, sports nutrient products are developed. The use of these protein supplements in diet has increased the physiological requirement of protein but whether the use of these supplements is having positive or negative effect on the host is still a topic of debate for many. Large amount of protein derived constituents end up in the large intestine in case of high protein diets as compared to medium or low protein diets, leading to more bacterial amino acid metabolism in colon having positive as well as negative effects on the host. We, here, will try to summarize the impact of protein diet supplements on athletes.

Influence of varying degrees of malnutrition on IGF-I expression in the rat diaphragm

2003 ◽  
Vol 95 (2) ◽  
pp. 555-562 ◽  
Author(s):  
Michael I. Lewis ◽  
Hongyan Li ◽  
Zhi-Shen Huang ◽  
Manmohan S. Biring ◽  
Bojan Cercek ◽  
...  
Keyword(s):  
Muscle Protein ◽  
Muscle Growth ◽  
The Body ◽  
Diaphragm Muscle ◽  
Fiber Types ◽  
Mrna Levels ◽  
Adult Rats ◽  
Cross Sectional ◽  
Igf I ◽  
The Impact

This study evaluated the impact of varying degrees of prolonged malnutrition on the local insulin-like growth factor-I (IGF-I) system in the costal diaphragm muscle. Adult rats were provided with either 60 or 40% of usual food intake over 3 wk. Nutritionally deprived (ND) animals (i.e., ND60 and ND40) were compared with control (Ctl) rats fed ad libitum. Costal diaphragm fiber types and cross-sectional areas were determined histochemically. Costal diaphragm muscle IGF-I mRNA levels were determined by RT-PCR. Serum and muscle IGF-I peptide levels were determined by using a rat-specific radioimmunoassay. The body weights of Ctl rats increased by 5%, whereas those of ND60 and ND40 animals decreased by 16 and 26%, respectively. Diaphragm weights were reduced by 17 and 27% in ND60 and ND40 animals, respectively, compared with Ctl. Diaphragm fiber proportions were unaffected by either ND regimen. Significant atrophy of both type IIa and IIx fibers was noted in the ND60 group, whereas atrophy of all three fiber types was observed in the diaphragm of ND40 rats. Serum IGF-I levels were reduced by 62 and 79% in ND60 and ND40 rats, respectively, compared with Ctl. Diaphragm muscle IGF-I mRNA levels in both ND groups were similar to those noted in Ctl. In contrast, IGF-I concentrations were reduced by 36 and 42% in the diaphragm muscle of ND60 and ND40 groups, respectively, compared with Ctl. We conclude that the local (autocrine/paracrine) muscle IGF-I system is affected in our models of prolonged ND. We propose that this contributes to disordered muscle protein turnover and muscle cachexia with atrophy of muscle fibers. This is particularly so in view of recent data demonstrating the importance of the autocrine/paracrine system in muscle growth and maintenance of fiber size.

scholarly journals Prenatal Skeletal Muscle Transcriptome Analysis Reveals Novel MicroRNA-mRNA Networks Associated with Intrauterine Growth Restriction in Pigs

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1007
Author(s):  
Asghar Ali ◽  
Eduard Murani ◽  
Frieder Hadlich ◽  
Xuan Liu ◽  
Klaus Wimmers ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fetal Growth ◽  
Intrauterine Growth Restriction ◽  
Muscle Development ◽  
Muscle Growth ◽  
The Body ◽  
Growth Restriction ◽  
Economic Losses ◽  
Biological Processes ◽  
Intrauterine Growth

Intrauterine growth restriction (IUGR) occurs in 15–20% of pig neonates and poses huge economic losses to the pig industry. IUGR piglets have reduced skeletal muscle growth, which may persist after birth. Prenatal muscle growth is regulated by complex molecular pathways that are not well understood. MicroRNAs (miRNAs) have emerged as the main regulators of vital pathways and biological processes in the body. This study was designed to identify miRNA–mRNA networks regulating prenatal skeletal muscle development in pigs. We performed an integrative miRNA–mRNA transcriptomic analysis in longissimus dorsi muscle from IUGR fetuses and appropriate for gestational age (AGA) fetuses at 63 days post conception. Our data showed that 47 miRNAs and 3257 mRNAs were significantly upregulated, and six miRNAs and 477 mRNAs were significantly downregulated in IUGR compared to AGA fetuses. Moreover, 47 upregulated miRNAs were negatively correlated and can potentially target 326 downregulated genes, whereas six downregulated miRNAs were negatively correlated and can potentially target 1291 upregulated genes. These miRNA–mRNA networks showed enrichment in biological processes and pathways critical for fetal growth, development, and metabolism. The miRNA–mRNA networks identified in this study can potentially serve as indicators of prenatal fetal growth and development as well as postnatal carcass quality.

Growth of bovine tissues 2. Genetic influences on muscle growth and distribution in young bulls

1978 ◽  
Vol 27 (1) ◽  
pp. 51-61 ◽  
Author(s):  
R. T. Berg ◽  
B. B. Andersen ◽  
T. Liboriussen
Keyword(s):  
Muscle Development ◽  
Muscle Growth ◽  
Young Male ◽  
Live Weight ◽  
Allometric Equation ◽  
Growth Patterns ◽  
The Body ◽  
Muscle Weight ◽  
Tissue Separation ◽  
Total Muscle

ABSTRACTMuscle weight distribution was compared by jointing and complete tissue separation of carcasses from 277 young male progeny of eight sire breeds and two dam breeds, serially slaughtered at 300 kg live weight, 12 months and 15 months of age. The sire breeds were Simmental, Charolais, Danish Red and White, Romagnola, Chianina. Hereford, Blonde d'Aquitaine and Limousin; the dam breeds were Danish Red and Danish Black Pied.Growth impetus for muscle in each joint was established from the allometric equation (Y = aXb). Growth coefficients, b, were homogenous among breeds, indicating similar muscle development patterns over the range of weights studied. Growth impetus waves increased centripetally on the limbs, caudocephalically on the whole of the body (being more marked dorsally) and dorsoventrally on the trunk.There were small but significant breed differences in the proportion of muscle in different joints at similar total muscle weight. They probably reflect differences in maturity and other minor functional influences. Chianina and Hereford crosses were the two extremes for muscle distribution with many of the remaining breed groups being very similar. Differences were already established by 300 kg live weight and muscle growth patterns were similar among breeds over the range of the experiment.

scholarly journals Effects of oxidative stress during human and animal reproductions: A review

2015 ◽  
Vol 08 (01) ◽  
pp. 006-011
Author(s):  
Carolina B. Berchieri-Ronchi ◽  
Paula T. Presti ◽  
Ana Lucia A. Ferreira ◽  
Camila R. Correa ◽  
Daisy Maria F. Salvadori ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Deoxyribonucleic Acid ◽  
Experimental Studies ◽  
The Body ◽  
Defense Strategies ◽  
Reproduction Process ◽  
Reproduction Period ◽  
Cellular Components ◽  
The Impact

ABSTRACTGiven its high ability to damage important cellular components (lipids, proteins and deoxyribonucleic acid), oxidative stress is now recognized as one of the most common mechanisms associated with development of a variety of diseases and natural events such as pregnancy. During reproduction period, there is a change in the pro-oxidant and antioxidant balance due to the body and circulation modifications that are inherent to the pregnancy process. The present paper discusses the role of oxidative stress on the reproduction process. More effective defense strategies are needed to decrease the deleterious effects of oxidative-stress-induced gestation. This approach could be achieved by antioxidant status alteration. Further clinical and experimental studies are needed for better understanding of oxidative stress mechanism and the impact of antioxidant supplementation on reproduction.

scholarly journals The intestinal microbiota contributes to the growth and physiological state of muscle tissue in piglets

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Renli Qi ◽  
Jing Sun ◽  
Xiaoyu Qiu ◽  
Yong Zhang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Muscle Tissue ◽  
Muscle Development ◽  
Fecal Microbiota Transplantation ◽  
Physiological State ◽  
Fatty Acid Content ◽  
Muscle Growth ◽  
Fecal Microbiota ◽  
The Body ◽  
And Function

AbstractAlthough the importance of the intestinal microbiota in host growth and health is well known, the relationship between microbiota colonization and muscle development is unclear. In this study, the direct causal effects of the colonization of gut microorganisms on the muscle tissue of piglets were investigated. The body weight and lean mass of germ-free (GF) piglets were approximately 40% lower than those of normal piglets. The deletion of the intestinal microbiota led to weakened muscle function and a reduction in myogenic regulatory proteins, such as MyoG and MyoD, in GF piglets. In addition, the blinded IGF1/AKT/mTOR pathway in GF piglets caused muscle atrophy and autophagy, which were characterized by the high expression of Murf-1 and KLF15. Gut microbiota introduced to GF piglets via fecal microbiota transplantation not only colonized the gut but also partially restored muscle growth and development. Furthermore, the proportion of slow-twitch muscle fibers was lower in the muscle of GF piglets, which was caused by the reduced short-chain fatty acid content in the circulation and impaired mitochondrial function in muscle. Collectively, these findings suggest that the growth, development and function of skeletal muscle in animals are mediated by the intestinal microbiota.

scholarly journals USE OF DIGITALIZATION MEANS IN THE FORMATION OF THE MICROCLIMATE OF ANIMAL PREMISES

2021 ◽  
pp. 136-140
Author(s):  
Olena Trukhanska
Keyword(s):  
Positive Impact ◽  
Animal Health ◽  
Animal Husbandry ◽  
The Body ◽  
Automated Control ◽  
High Productivity ◽  
Air Exchange Rate ◽  
Software Products ◽  
Heat Shielding ◽  
The Impact

The study of information systems and technologies, specialized software products aimed at solving the problems of animal husbandry management and determining the prospects for their use by specialists is relevant. The main factor determining the life of animals is the environment. One of the ways to intensify livestock (pigs, cattle, poultry, etc.) is a comprehensive automation of labor-intensive technological processes, including the formation of the microclimate of livestock facilities, which would have a positive impact on animal health, productivity and product quality. However, animals are also affected by certain methods and methods of feeding, man-made content, zonal climate, heat-shielding properties of enclosing structures, air exchange rate, ventilation efficiency, sewage condition, manure removal and removal methods, lighting. The impact of microclimate on animals consists of a set of environmental factors: temperature, humidity, air velocity, chemical composition, microbial and dust pollution, lighting, etc. The main parameter of the air environment is temperature. This has the greatest impact on animal health and productivity. The hygienic value of temperature is that it affects the thermoregulation of the body, which is divided into the processes of chemical thermoregulation - the reaction of heat generation and the processes of physical thermoregulation - the regulation of heat transfer. The study of the technology of improving the efficiency of microclimate regulation in livestock premises based on the use of an automated control system is promising. Thus, changing the composition and properties of the environment, you can affect the body of animals, achieving high productivity.

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