scholarly journals Wasabi Japonica antimicrobial effect on pathogenic bacteria and microbiological quality of salmon sushi samples in two establishments in the city of Toledo – PR, Brazil

2015 ◽  
Vol 6 (3) ◽  
pp. 65
Author(s):  
Júlia Maria Tonin Geiss ◽  
Fernanda Luisa Guaitanelly Heringer ◽  
Juliana Bernardi Wenzel
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Antimicrobial Effect ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Disk Diffusion Test ◽  
Contaminated Food ◽  
Sanitary Conditions ◽  
The City

<p>The foodborne illnesses are attributed to ingestion of contaminated food, mainly due to improper handling and lack of sanitary conditions. Foods that are handled and eaten raw, such as traditional Japanese food, sushi and sashimi are targets of global concern. So it is necessary to analyze them to identify microbiological contamination and thus prevent them. The Wasabi Japonica is traditionally consumed with these foods and may have antimicrobial activity. Therefore, the aim of this study was to evaluate the microbiological quality of salmon sushi sold in the city of Toledo - PR and verify the antimicrobial effect of 'wasabi' on pathogenic bacteria. We investigated Salmonella spp., Bacillus cereus, Escherichia coli and Staphylococcus aureus, in samples of salmon sushi and determined the antimicrobial effect of wasabi Japonica, by determining the Minimum Inhibitory Concentration and the disk diffusion test (antibiogram). It was concluded that all the samples of salmon sushi analyzed were considered satisfactory and safe for human consumption, with good quality and within the hygienic-sanitary parameters established by law. Furthermore, the bacteriostatic action was confirmed in the "wasabi" against the pathogens that are possibly detected in these foods.</p>

scholarly journals Microbiological quality of frozen black mussels (Mytilus galloprovincialis, Lamarck, 1819) purchased from markets in the İzmir Province of Turkey

2021 ◽  
Vol 38 (2) ◽  
pp. 167-172
Author(s):  
Bülent Kafa ◽  
Berna Kılınç
Keyword(s):  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Mytilus Galloprovincialis ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Consumer Safety ◽  
Vibrio Spp

The purpose of this study was to examine the microbiological quality and consumer safety of frozen black mussels purchased from four different markets (A, B, C, D) in the İzmir Province of Turkey. A total of 36 frozen black mussel packages, which contained approximately 50 mussels in each, were purchased from the markets. They were not only examined for total mesophilic, psychrotrophic, coliform and fecal coliform bacteria count but also investigated for pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Vibrio spp. According to the results of the mesophilic bacteria counts, frozen black mussels did not exceed the microbiological limits set for safe consumption. The findings showed that frozen black mussels purchased from (A, B, C, D) of markets were safe for human consumption because pathogenic bacteria species such as E.coli, Salmonella spp., S. aureus, L. monocytogenes and Vibrio spp. were not present in the frozen mussel samples.

scholarly journals Research of microorganisms, parasites and dirt in drumsticks sold by street vendors

2020 ◽  
Vol 9 (12) ◽  
pp. e35291210737
Author(s):  
Maria das Graças do Carmo ◽  
Renata Cristina Borges da Silva Macedo ◽  
José Lucas Girão Rabelo ◽  
Flávio Estefferson de Oliveira Santana ◽  
Bruno Sueliton dos Santos ◽  
...  
Keyword(s):  
Human Consumption ◽  
Salmonella Spp ◽  
Street Vendors ◽  
Mesophilic Bacteria ◽  
A Value ◽  
Thermotolerant Coliforms ◽  
Mold And Yeast ◽  
Sanitary Conditions ◽  
The City

It is of great importance to maintain the hygienic-sanitary quality of food and places where it is handled and marketed. This work aimed to evaluate the hygienic-sanitary conditions of Brazilian croquettes with chicken filling sold by street vendors in the city of Mossoró, state of Rio Grande do Norte. Ten samples were subjected to counting aerobic mesophilic bacteria, counting fungi and yeasts, Salmonella spp., Coliforms at 35ºC and 45ºC. The research was into find dirtiness and pathogenic parasites was carried out with the flotation and sedimentation test. Total coliforms were found in 30% (3/10) of the samples evaluated, with values ​​ranging from <3.0 to> 1100 NMP / g and the presence of thermotolerant coliforms in 10% (1/10) with a value greater than > 1100 NMP / g. The count of mesophilic bacteria varied from 3.17 to 6.23 (Log10 UFC/g). While the mold and yeast count between 3.69 to 5.55 (Log10 UFC/g). Regarding the analyzes of Salmonella ssp., Escherichia coli and pathogenic parasites were negative. The results allowed to conclude that, of the total of ten samples analyzed, 10% (1/10) was unfit for human consumption.

scholarly journals Microbiological Quality of Egusi Pudding, A Traditional Cake of Cucurbitaceae Sold in the City of Yaoundé, Cameroon

2022 ◽  
Vol 2022 ◽  
pp. 1-12
Author(s):  
Ronice Zokou ◽  
Hippolyte Tene Mouafo ◽  
Julie Mathilde Klang ◽  
Noutsa Boris Simo ◽  
Raymond Simplice Mouokeu ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Threshold Value ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Protein Sources ◽  
Staphylococcus Spp ◽  
And Control ◽  
The City

Egusi pudding is one of the most popular traditional dishes of the Cameroonian population. Besides its nutritional values, it is also endowed with a sociocultural character. Nowadays, consumer demand for Egusi pudding has increased and the dish is sold as street food in several cities of Cameroon and mostly under uncontrolled hygienic conditions. The objective of this study was to assess the microbiological quality of Egusi pudding sold in the city of Yaoundé taking into consideration the protein sources and the sampling sites. Five types of Egusi pudding samples based on protein sources were randomly collected from 25 sellers distributed in 7 districts in the city of Yaoundé and their microbiological quality were assessed. The results showed that the total aerobic count of the different samples (2.97 ± 0.03 to 4.43 ± 0.05 Log CFU/g) was under the threshold value (5.47 Log CFU/g) recommended for food intended for human consumption. Loads of fecal coliforms (1.47 ± 0.00 to 5.47 ± 0.00 Log CFU/g) in 40% of samples, Escherichia coli (2.39 ± 0.12 to 5.43 ± 0.05 Log CFU/g) in 60% of samples, fecal streptococci (2.90 ± 0.15 to 4.74 ± 0.05 Log CFU/g) in 88% of samples, Pseudomonas spp. (3.39 ± 0.15 to 5.43 ± 0.06 Log CFU/g) in 100% of samples, and the presence of Salmonella spp. in 56% of samples revealed a poor level of hygiene of the vendors. Pathogens associated with unsafe food handling such as Staphylococcus spp. were found in 100% of samples at loads (3.84 ± 0.18 to 5.43 ± 0.05 Log cfu/g) higher than the norms of the European Commission. Potential toxigenic pathogens such as Clostridium perfringens, Yersinia enterocolitica, Bacillus cereus, and moulds were also found, respectively, in 100, 96, 96, and 100% of samples. Overall, the most contaminated samples were those made with sardine as protein source, 92.85% (n = 23/25), followed with beef (88.57%), mackerel (84.28%), cod (82.85%), and control (77.1%). The results of this study suggest that important measures should be taken by the Public Health Service in order to sensitize the producers and vendors of Egusi pudding on the respect of good hygiene, manufacturing practices, and the continuous monitor of the quality of traditional products sold in markets.

scholarly journals EVOLUÇÃO DA QUALIDADE MICROBIOLÓGICA DE QUEIJO MINAS FRESCAL COMERCIALIZADO EM CURITIBA (PR) NO INTERVALO DE 10 ANOS (1999 E 2009) Assessment of the microbiological quality of Minas frescal cheese commercialized in the city of Curitiba, Parana State, Brazil at 10 years’ interval (1999 and 2009)

2012 ◽  
Vol 10 (3) ◽  
pp. 243 ◽  
Author(s):  
Hanna Lethycia Wolupeck ◽  
Helen Caroline Raksa ◽  
Luciane Silvia Rossa ◽  
Raquel Biasi ◽  
Renata Ernlund Freitas de Macedo
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
E Coli ◽  
The City

O queijo Minas frescal é um dos mais populares do Brasil, porém o alto teor de umidade associado ao métodode processamento, muitas vezes artesanal, e de armazenamento desse produto o tornam muito perecível.Este trabalho teve como objetivo avaliar e comparar a qualidade microbiológica de queijo Minas frescalcomercializado na cidade de Curitiba (PR) nos anos de 1999 e 2009, verificando a evolução na qualidadehigiênico-sanitária desse produto no período de 10 anos. Foram analisadas 11 marcas comerciais de queijo Minas frescal disponíveis no comércio varejista da cidade de Curitiba, sendo amostradas cinco unidades de cada marca, totalizando 55 amostras. Os queijos foram submetidos à pesquisa de Salmonella spp., contagem de coliformes totais e Escherichia coli, contagem de Staphylococcus coagulase positiva e contagem de aeróbios mesófilos, com resultados expressos em UFC/g. Das 55 amostras de queijo, 41,82% e 78,18% apresentaram contagem de E. coli e de coliformes totais acima do limite permitido, respectivamente. Somente uma amostra (1,82%) do total avaliado mostrou-se em desacordo com os padrões para S. coagulase positiva e uma para Salmonella spp. Ambas as amostras foram adquiridas em 2009. Todas as amostras avaliadas em 2009 apresentaram elevada contagem de aeróbios mesófilos, revelando alta carga microbiana. Comparativamente, os queijos avaliados em 1999 mostraram qualidade microbiológica superior aos queijos avaliados em 2009 (p < 0,05). Destes, 100% apresentaram no mínimo um parâmetro microbiológico em desacordo com a legislação vigente, indicando que a qualidade dos queijos Minas frescal avaliados em 2009 apresentou-se inferior a dos queijos avaliados em 1999.

scholarly journals Microbiological Quality of Drinking Water in Sachets Sold in the City of Yaoundé in Cameroon: Tests of Sensitivity to the Antibiotics of Bacteria Isolated

2020 ◽  
Vol 8 (1) ◽  
pp. 27
Author(s):  
Nguepidjo Gilbert ◽  
Kapso Tchouankep Mireille ◽  
Ngong Ankiambom Innocent ◽  
Tonmeu Douyong Chimène Sandrine ◽  
Enoka Patrice ◽  
...  
Keyword(s):  
Drinking Water ◽  
Low Cost ◽  
Bacterial Species ◽  
Microbiological Quality ◽  
Positive Culture ◽  
Human Beings ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
The City

Water is the basic drink for human beings and drinking water in sachets is very popular because of its relatively low cost and availability. The aim of this study is to determine the bacteriological profile of sachet drinking water sold in the city of Yaounde. It was a descriptive cross-sectional study covering the period from March to June 2019, carried out in the application laboratory of ETMS-Yaounde. A total of 230 samples of drinking water in sachets purchased in different markets in the city of Yaounde were analyzed using Mac Conkey's flooding method. The identification was done on the API 20 E Gallery and the susceptibility test on Mueller Hinton media. The size of the sample was 230 packaged sachet drinking water and 213 of the 230 revealed 92% of positive culture of germs, and only 17 samples gave a negative culture, at a percentage of 8% of isolated germs. The isolated bacterial species and their respective abundances in samples were Enterobacter gergoviae (3%), Klebsiella pneumoniae (5%), Proteus mirabilis (5%), Serratia fonticola (5%), Salmonella choler arizonae cloacae (8%), Salmonella spp. (8%), Enterobacter cloacae (10%), Staphylococcus aureus (10%), Pseudomonas aeruginosa (18%) and Staphylococcus epidermidis (28%). All these tested germs were resistant to Amoxicillin and Erythromycin and 70% of tested germs were sensitive to Gentamycin. Overall, the results revealed poor microbiological quality of these waters. This exposes consumers to health risks, and it is important to inform and sensitize consumers about the risks involved, to educate producers and to control their activities by the health services.

scholarly journals Chicken gizzards: A study of the prevalence of Salmonella spp.

2014 ◽  
Vol 4 (2) ◽  
pp. 66
Author(s):  
Ana Paula Brizio ◽  
Ana Gabriela Anthero
Keyword(s):  
Microbiological Quality ◽  
Culture Method ◽  
Human Consumption ◽  
Consumer Health ◽  
Limited Information ◽  
Rio Grande Do Sul ◽  
Salmonella Spp ◽  
Chicken Gizzard ◽  
Inspection Service

<p>Despite the large amount of giblets marketed in Brazil, there is currently limited information regarding the hygienic quality of these products. In view of this situation, this study aimed to determine the prevalence of <em>Salmonella</em> spp.&nbsp; in chilled chicken gizzard destined for human consumption. The research was conducted in slaughterhouses under Federal Inspection Service, located in Rio Grande do Sul state, with a sampling of 48 chilled chicken gizzard samples, which were analyzed for <em>Salmonella</em> spp. through the traditional culture method. All samples tested were negative for the pathogen. Thus, it can be concluded that the chicken giblets analyzed showed satisfactory microbiological quality and do no risk to consumer health.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v4i2.123</p>

Microbiological Quality and Prevalence of β-Lactam Antibiotic Resistance Genes in Oysters (Crassostrea rhizophorae)

2017 ◽  
Vol 80 (3) ◽  
pp. 488-496 ◽  
Author(s):  
Maria Aparecida da RessurreiÇão Brandão ◽  
Amanda Teixeira Sampaio Lopes ◽  
Maria Tereza da Silva Neta ◽  
Rhyan Barros Farias de Oliveira ◽  
Rachel Passos Rezende ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Antibiotic Resistance Genes ◽  
Human Consumption ◽  
E Coli ◽  
Lactam Antibiotic ◽  
Crassostrea Rhizophorae

ABSTRACTThe microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA. Our results detected total coliforms, Escherichia coli, and enterobacteria in the oysters from urban estuaries, which is indicative of poor water quality. In addition, our detection of the eaeA and stxA2 virulence genes in 16.7% of E. coli isolates from oysters from this region suggests the presence of multiantibiotic-resistant enteropathogenic and enterohemorrhagic E. coli strains. During periods of low precipitation, increased contamination by E. coli (in winter) and Vibrio parahaemolyticus (in autumn) was observed. In contrast, cultivated oysters inhabiting monitored farms in preserved areas had low levels of bacterial contamination, emphasizing that oyster culture monitoring enhances food quality and makes oysters fit for human consumption. Distinct antibiotic resistance profiles were observed in bacteria isolated from oysters collected from different areas, including resistance to β-lactam antibiotics. The presence of the blaTEM gene in 91.3% of oyster samples indicated that microorganisms in estuarine water conferred the capability to produce β-lactamase. To our knowledge, this is the first study to directly quantify and detect β-lactam antibiotic resistance genes in oysters. We believe our study provides baseline data for bacterial dynamics in estuarine oysters; such knowledge contributes to developing risk assessments to determine the associated hazards and consequences of consuming oysters from aquatic environments containing pathogenic bacteria that may possess antibiotic resistance genes.

scholarly journals Microbiological quality of surimi-based imitation crab

2018 ◽  
Vol 51 (2) ◽  
pp. 134
Author(s):  
N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽  
A. ABRAHIM (Α. ΑΜΠΡΑΧΙΜ) ◽  
E. THEOLOGIDOU (Ε. ΘΕΟΛΟΓΙΔΟΥ) ◽  
Pr. KARAIOANNOGLOU (ΠΡ. ΚΑΡΑΪΩΑΝΝΟΓΛΟΥ) ◽  
A. KANSOUZIDOU (Α. ΚΑΝΣΟΥΖΙΔΟΥ)
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Storage Period ◽  
Coliform Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Microbial Indices

A survey was conducted to evaluate the microbiological quality of sliced, vacuumpacked, surimi-based imitation crab at the retail level, during a 3-month storage period. The roll shaped product imported from France, was sliced and packed in a fishery processing plant in the region of Thessaloniki. Twenty five samples were examined each month, and a total of 75 samples were examined during the three month storage period of the product. Psychrotropic, lactic acid and coliform bacteria, Escherichia coli I, yeasts, Staphylococcus aureus, Salmonella spp., Listeria spp. and Escherichia coli Θ157:Η7, were used as microbial indices, to evaluate the microbiological quality of the product. The following conclusions were derived from the evaluation of the results of the microbiological examinations. 1) The microbiological condition of the crab meat analogs examined on the 15th day of storage, was found satisfactory, since microbial populations were very low, while pathogenic bacteria were not detected in any of the examined samples. 2) The overall microbiological quality of the samples examined at the end of the second month of storage, was also good, since psychrotrophic and lactic acid bacteria exceeded 1Θ7 log cfu/g only in one sample), while coliform bacteria were not detected in 60% of the samples and Escherichia coli I was detected only in one sample. The mean value of the yeasts was 2.84 log cfu/g, while molds were not detected in any of samples examined. 3) The microbiological quality of the samples examined at the end of the three months storage period was not satisfactory, since the microbial indices in the majority of the samples (80%), were rather high. S. aureus, Salmonella spp, Listeria s pp. and E. coli 0157:H7 were not detected in any sample, during the three month storage period.

scholarly journals Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic

2020 ◽  
Author(s):  
Gwladys S. Komagbe ◽  
Philippe Sessou ◽  
François Dossa ◽  
Prudencio Sossa-Minou ◽  
Bernard Taminiau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Compliance Rate ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Salmonella Spp ◽  
Potential Health ◽  
Antibiotic Susceptibility Test ◽  
Aerobic Flora

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.

Assessment of the Microbiological Quality of Ready-To-Use Vegetables for Health-Care Food Services

1997 ◽  
Vol 60 (8) ◽  
pp. 954-960 ◽  
Author(s):  
JOSEPH A. ODUMERU ◽  
SHEILA J. MITCHELL ◽  
DAVID M. ALVES ◽  
JOHN A. LYNCH ◽  
ARLENE J. YEE ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Food Services ◽  
Salmonella Spp ◽  
E Coli ◽  
Vegetable Samples ◽  
Serotype O ◽  
Select Group ◽  
Before And After

The microbiological quality of ready-to-use (RTU) vegetables, including chopped lettuce, salad mix, carrot sticks, cauliflower florets, sliced celery, coleslaw mix, broccoli florets, and sliced green peppers was determined before and after processing. Microbial profiles were obtained 24 h after processing and on days 4, 7, and 11 after storage at 4 and 10°C to simulate temperature abuse. In addition, the microbial profiles of four RTU vegetables, coleslaw mix, salad mix, cauliflower florets, and sliced green peppers were determined 7 days after distribution to a select group of Ontario hospitals. RTU vegetables, with the exception of green peppers, showed up to a 1-log decrease in aerobic colony counts after processing. These counts increased to preprocessing levels after 4 days of storage at both 4 and 10°C. RTU vegetables stored at temperature abuse conditions (10°C)had significantly higher counts (P &lt; 0.001) on days 4 to 11 as compared to those stored at 4°C. Green peppers had the highest bacterial counts while cauliflower and chopped lettuce had the lowest counts at both storage temperatures (P &lt; 0.05). Increased levels of Listeria monocytogenes in RTU vegetables were associated with temperature abuse. Levels of &gt;100 MPN/g for L. monocytogenes were detected in 8 of 120 (6.7%) samples stored at 10°C but not in 175 samples stored at 4°C after 7 days (P &lt; 0.05). Overall, L. monocytogenes was detected in 13 of 120 (10.8%) RTU vegetables stored for up to 11 days at 10°C and 5 of 176 (2.8%) samples stored at 4°C (P &lt; 0.05). E. coli was detected in 2 of the 120 (1.7%) processed RTU vegetables after day 7 of storage at 10°C and 1 of the 65 (1.5%) unprocessed vegetables from the same batches of vegetables used for processing. This indicator organism was not detected in RTU vegetable samples stored at 4°C or in any of the RTU vegetable samples obtained from hospital coolers. Other pathogenic bacteria, such as Salmonella spp., Campylobacter spp., Yersinia enterocolitica (serotype O:3) and verocytotoxigenic E. coli (VTEC) were not detected in any of the RTU vegetables tested, Recommendations regarding processing, distribution, and storage of these products are presented.

Sign in / Sign up

Export Citation Format

Share Document