scholarly journals Microbiological Quality of Drinking Water in Sachets Sold in the City of Yaoundé in Cameroon: Tests of Sensitivity to the Antibiotics of Bacteria Isolated

2020 ◽  
Vol 8 (1) ◽  
pp. 27
Author(s):  
Nguepidjo Gilbert ◽  
Kapso Tchouankep Mireille ◽  
Ngong Ankiambom Innocent ◽  
Tonmeu Douyong Chimène Sandrine ◽  
Enoka Patrice ◽  
...  
Keyword(s):  
Drinking Water ◽  
Low Cost ◽  
Bacterial Species ◽  
Microbiological Quality ◽  
Positive Culture ◽  
Human Beings ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
The City

Water is the basic drink for human beings and drinking water in sachets is very popular because of its relatively low cost and availability. The aim of this study is to determine the bacteriological profile of sachet drinking water sold in the city of Yaounde. It was a descriptive cross-sectional study covering the period from March to June 2019, carried out in the application laboratory of ETMS-Yaounde. A total of 230 samples of drinking water in sachets purchased in different markets in the city of Yaounde were analyzed using Mac Conkey's flooding method. The identification was done on the API 20 E Gallery and the susceptibility test on Mueller Hinton media. The size of the sample was 230 packaged sachet drinking water and 213 of the 230 revealed 92% of positive culture of germs, and only 17 samples gave a negative culture, at a percentage of 8% of isolated germs. The isolated bacterial species and their respective abundances in samples were Enterobacter gergoviae (3%), Klebsiella pneumoniae (5%), Proteus mirabilis (5%), Serratia fonticola (5%), Salmonella choler arizonae cloacae (8%), Salmonella spp. (8%), Enterobacter cloacae (10%), Staphylococcus aureus (10%), Pseudomonas aeruginosa (18%) and Staphylococcus epidermidis (28%). All these tested germs were resistant to Amoxicillin and Erythromycin and 70% of tested germs were sensitive to Gentamycin. Overall, the results revealed poor microbiological quality of these waters. This exposes consumers to health risks, and it is important to inform and sensitize consumers about the risks involved, to educate producers and to control their activities by the health services.

scholarly journals EVOLUÇÃO DA QUALIDADE MICROBIOLÓGICA DE QUEIJO MINAS FRESCAL COMERCIALIZADO EM CURITIBA (PR) NO INTERVALO DE 10 ANOS (1999 E 2009) Assessment of the microbiological quality of Minas frescal cheese commercialized in the city of Curitiba, Parana State, Brazil at 10 years’ interval (1999 and 2009)

2012 ◽  
Vol 10 (3) ◽  
pp. 243 ◽  
Author(s):  
Hanna Lethycia Wolupeck ◽  
Helen Caroline Raksa ◽  
Luciane Silvia Rossa ◽  
Raquel Biasi ◽  
Renata Ernlund Freitas de Macedo
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
E Coli ◽  
The City

O queijo Minas frescal é um dos mais populares do Brasil, porém o alto teor de umidade associado ao métodode processamento, muitas vezes artesanal, e de armazenamento desse produto o tornam muito perecível.Este trabalho teve como objetivo avaliar e comparar a qualidade microbiológica de queijo Minas frescalcomercializado na cidade de Curitiba (PR) nos anos de 1999 e 2009, verificando a evolução na qualidadehigiênico-sanitária desse produto no período de 10 anos. Foram analisadas 11 marcas comerciais de queijo Minas frescal disponíveis no comércio varejista da cidade de Curitiba, sendo amostradas cinco unidades de cada marca, totalizando 55 amostras. Os queijos foram submetidos à pesquisa de Salmonella spp., contagem de coliformes totais e Escherichia coli, contagem de Staphylococcus coagulase positiva e contagem de aeróbios mesófilos, com resultados expressos em UFC/g. Das 55 amostras de queijo, 41,82% e 78,18% apresentaram contagem de E. coli e de coliformes totais acima do limite permitido, respectivamente. Somente uma amostra (1,82%) do total avaliado mostrou-se em desacordo com os padrões para S. coagulase positiva e uma para Salmonella spp. Ambas as amostras foram adquiridas em 2009. Todas as amostras avaliadas em 2009 apresentaram elevada contagem de aeróbios mesófilos, revelando alta carga microbiana. Comparativamente, os queijos avaliados em 1999 mostraram qualidade microbiológica superior aos queijos avaliados em 2009 (p < 0,05). Destes, 100% apresentaram no mínimo um parâmetro microbiológico em desacordo com a legislação vigente, indicando que a qualidade dos queijos Minas frescal avaliados em 2009 apresentou-se inferior a dos queijos avaliados em 1999.

scholarly journals Point-of-Use Water Treatment Methods and the Microbiological Quality of Drinking Water in a Peri-Urban Community of Bonendale, Douala IV, Cameroon

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ngouakam H ◽  
◽  
Fru Cho J ◽  
Tientche B ◽  
◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Cross Sectional ◽  
Potential Health ◽  
Coliform Count ◽  
Point Of Use ◽  
Dug Wells

Background: The study aimed at assessing the knowledge of Bonendale populations related to safe drinking water; determine the microbiological quality and the household treatment practices of drinking water at the point of use (PoU). Methods: The study was descriptive and cross-sectional, conducted between November 2017 and November 2018 in Bonendale, Douala IV. A systematic random sampling method was used to select 237 households. Results: The main source of drinking water in the study population was dug wells (64.9%). The major activity around the boreholes was agriculture 50% (6/12) while agriculture and livestock rearing were practiced around 34.3% (12/35) and 42.8% (15/35) of dug wells respectively. Nearly half 110 (46.4%) of respondents had a good knowledge of physical properties of drinking water. Fifty-nine water samples out of a total of 60 tested (98.3%) were contaminated by coliform bacteria. Analysis of water samples indicated that 47.6% (10/21) of dug wells, 37.0% (10/27) of Camwater, and 41.2% (5/12) of boreholes had a total coliform count varying between 101-1000 CFU/100mL. And only a single Camwater water sample 1 (137%) had a coliform count of 0 CFU/100mL. Conclusion: These findings indicated the poor microbiological quality of point water used by Bonendale populations representing an important potential health risk of water-borne diseases. The results also reveal that a very high proportion of households treat their PoU before drinking.

scholarly journals ANALYSIS OF THE FACTORS AFFECTING THE QUALITY OF THE CHEMICAL AND MICROBIOLOGICAL DRINKING WATER AT THE DEPOT DRINKING WATER REFILL TULUNGAGUNG DISTRICT

2018 ◽  
Vol 1 (1) ◽  
pp. 104-112
Author(s):  
Eka Puspitasari
Keyword(s):  
Drinking Water ◽  
Microbiological Quality ◽  
Sampling Technique ◽  
Survey Method ◽  
Test Results ◽  
Raw Water ◽  
Cross Sectional ◽  
Regression Test ◽  
Independent Variable

Refill drinking water Depot is an industry that does the processing of the raw water into drinking water and sell directly to the consumer. Mn metal and bacterial Coliform is an indicator of refill drinking water polluters. This research aims to find the factors influencing the quality of Microbiology and chemistry at the depot drinking water drinking water refill Tulungagung district. Research using survey method with Cross sectional approach is analytical. The sampling technique used was Simple Random Sampling with a sample of 89 depot drinking water refill. Technique of data analysis using Regression and Logistic regression test linear. Independent variable examined was the source of raw water, the depot operator hygiene of drinking water, hygiene, sanitation, which covers three aspects namely sanitary location, building and facility sanitation. The results showed from 89 depot examined known as much as 79.8% sanitation facilities are not qualified, the value of the MPN 59.55% of eligible and levels of Mn 100% qualified. From Regresilogistik test results between independent variable with the value of the MPN indicate significance of 0.008 < (α = 0.05) on sanitation facilities are variable. This means there are influences between facilities sanitation microbiological quality of drinking water. On Linear regression test results between independent variable with the value obtained significance levels of Mn 0.47 > ((α = 0.05) which means no influence between independent variables with the chemical quality of drinking water. The lack of sanitation facilities increases the potential for bacterial contamination through the operator. Therefore the availability of on-site sanitation depot drinking water is a major factor that affects the quality of drinking water microbiology refills.

scholarly journals Away-from-home drinking water consumption practices and the microbiological quality of water consumed in rural western Kenya

2011 ◽  
Vol 9 (4) ◽  
pp. 628-636 ◽  
Author(s):  
W. Onyango-Ouma ◽  
Charles P. Gerba
Keyword(s):  
Drinking Water ◽  
Water Consumption ◽  
Microbiological Quality ◽  
Cross Sectional ◽  
Western Kenya ◽  
Household Level ◽  
Point Of Use ◽  
Quality Of Water ◽  
Consumption Practices

A cross-sectional descriptive study was conducted to examine away-from-home drinking water consumption practices and the microbiological quality of water consumed in rural western Kenya. The study involved adults and schoolchildren. Data were collected using focus group discussions, questionnaire survey, observations, diaries and interviews. The findings suggest that away-from-home drinking water consumption is a common practice in the study area; however, the microbiological quality of the water consumed is poor. While some respondents perceive the water to be safe for drinking mainly because of the clear colour of the water, others are forced by circumstances to drink the water as it is owing to a lack of alternative safe sources. It is concluded that there is a need for new innovative approaches to address away-from-home drinking water consumption in resource-poor settings in order to complement and maximize the benefits of point-of-use water treatment at the household level.

scholarly journals Microbial assessment of bottled drinking water of Kathmandu valley

2013 ◽  
Vol 1 (2) ◽  
pp. 84-86 ◽  
Author(s):  
M Timilshina ◽  
I Dahal ◽  
B Thapa
Keyword(s):  
Drinking Water ◽  
Membrane Filtration ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Bottled Water ◽  
Kathmandu Valley ◽  
Who Guidelines ◽  
Cross Sectional

INTRODUCTION: Due to increased demand and consumption of bottled water in Kathmandu valley, there has been a growing concern about the microbiological quality of this product. The objective of this study was to assess the microbial contamination of heterotrophs and total coliform. MATERIALS AND METHODS: This cross sectional study was done in randomly selected 30 different commercial brand of bottled water available in commercial market of Kathmandu valley, Nepal. Total coliform and heterotrophic bacteria were enumerated by the membrane filtration and spread plate technique, respectively. RESULTS: Nineteen samples (63.3%) showed the heterotrophic bacterial count above the acceptable range (i.e. <50 cfu/ml) and 56.6% (n=17) samples showed the total coliforms count crossed the WHO guidelines (i.e. 0 cfu/ml). 83.3% (n=25) brands of bottled water were not safe for drinking as they exceed the heterotrophic and/or total coliform count above the WHO guidelines. CONCLUSIONS: The presence of heterotrophs and the coliform in the bottled water is a serious concern for public health. Concerned authority should prioritize this issue and continuously monitor the quality of mushrooming bottled water manufacturers in Kathmandu valley to provide safe drinking water to the population. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7399 Int J Infect Microbiol 2012;1(1):84-86

scholarly journals Kualitas air minum depot isi ulang di Banyumas

2017 ◽  
Vol 33 (1) ◽  
pp. 7
Author(s):  
Harry Abriandy ◽  
Dibyo Pramono ◽  
Susi Iravati
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Microbiological Quality ◽  
Water Source ◽  
Drinking Water Quality ◽  
Reservoir Quality ◽  
P Value ◽  
Cross Sectional ◽  
Environmental Hygiene

Drinking water quality of refill depots in BanyumasPurposeThis study was conducted to determine the association between water source, reservoir quality, environmental hygiene, hygiene behavioral handlers, filter size, filter quality, the use of disinfectants and the use of a coagulant with the microbiological quality of drinking water refill.MethodsThis analytic observational study used a cross-sectional design. The study population was drinking water depots that have implemented internal monitoring of drinking water quality, the selection of the sample using simple random sampling method, with the total sample of 162.ResultsA total of 140 DWD (86.42%) are eligible microbiological, 22 DWD (13.58%) are not eligible. The source of water that is the most widely used are water wells (53.09%), while the source of water from the taps and springs are used as much as 33.95% and 12.96%, respectively.ConclusionThere is no relationship between the water source, reservoir quality, environmental hygiene, hygiene behavioral handlers, the size of the filter, the use of disinfectants and the use of a coagulant with the microbiological quality of drinking water refill. There is a relationship between the quality of the filter with the microbiological quality of drinking water refill (p-value=0.0304 95%CI 1.006-10.525; PR=3.255).

scholarly journals Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic

2020 ◽  
Author(s):  
Gwladys S. Komagbe ◽  
Philippe Sessou ◽  
François Dossa ◽  
Prudencio Sossa-Minou ◽  
Bernard Taminiau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Compliance Rate ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Salmonella Spp ◽  
Potential Health ◽  
Antibiotic Susceptibility Test ◽  
Aerobic Flora

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.

scholarly journals Kualitas air minum isi ulang pada depot air minum di Kabupaten Manokwari Selatan

Cassowary ◽  
2020 ◽  
Vol 3 (1) ◽  
pp. 61-71
Author(s):  
Awliya Nur Marhamah ◽  
Budi Santoso ◽  
Budi Santoso
Keyword(s):  
Drinking Water ◽  
Reverse Osmosis ◽  
Microbiological Quality ◽  
Drinking Water Treatment ◽  
Quality Standard ◽  
Special Treatment ◽  
Human Beings ◽  
Water Treatment Process ◽  
Living Things

Water is a chemical compound that is very important for the life of human beings and other living things. Refill water is water that undergoes special treatment, among others, the process of chlorination, aeration, filtration and irradiation with ultraviolet light. The objectives of this study were to determine the quality of refill drinking water produced by refill drinking water depots in South Manokwari Regency based on physical, chemical and microbiological variables. The descriptive method was used in this study. Samples of refill drinking water were collected from 6 depots located in South Manokwari Regency.   Water samples are taken to the laboratory for chemical, physical and microbiological quality analyzes. Good quality refill drinking water at the kiosk is drinking water produced by depots B and F, while drinking water produced from depot E was quite good.. The refill drinking water treatment process was carried out using the reverse osmosis method, a combination of the reverse osmosis and ultraviolet methods. The quality of refill drinking water in depots B, D, and F meets the quality standard requirements based on Permenkes 2010. E. coli population contained in refill water in depots A, C and F after storage in gallons for 3 days was above the quality standard requirements so it must be boiled before being consumed by humans.

Analisis Kualitas Mikrobiologi Air Minum Isi Ulang di Depo Air Minum Isi Ulang Kelurahan Jemur Wonosari Kota Surabaya

2021 ◽  
Vol 7 (1) ◽  
pp. 159-166
Author(s):  
Ajeng Dwi Rahmawati ◽  
Akas Yekti Pulih Asih ◽  
Edza Aria Wikurendra
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Laboratory Tests ◽  
Laboratory Data ◽  
Microbiological Quality ◽  
Microbiological Analysis ◽  
Cross Sectional ◽  
Drinking Water Samples

Background: Based on the preliminary test using laboratory tests from three refill drinking water samples in Jemur Wonosari Village, all three were positive for Coliform. Therefore, researchers are interested in researching Analysis of the Microbiological Quality of Refilled Drinking Water at the Refill Drinking Water Depot in Jemur Wonosari Village. Objectives: Knowing the microbiological quality of refill drinking water produced at the refill drinking water depot in Jemur Wonosari Village. Research Methods: This research is a descriptive study with a cross-sectional quantitative approach using primary and secondary data sources. The population in this study were all 13 refill drinking water depots. The research takes the total population. Refillable drinking water samples were tested at the Gayung Sari Regional Health Service Laboratory. Data analysis used descriptive analysis by comparing the results of laboratory tests with Permenkes 492 of 2010. Results: The results showed that 13 samples found that 13 samples were positive for Coliform, and 13 samples were negative for Escherichia coli. According to Permenkes No. 429 of 2010, the maximum limit per 100 ml of sample is 0. So the 13 samples of refill drinking water based on microbiological parameters, namely Escherichia coli in refilled drinking water, meet the standard while the Coliform does not meet the standard. Conclusion: All refill drinking water depots in Keluraham Jemur Wonosari produce refilled drinking water whose quality does not meet the government's microbiological standards. There is a need for increased supervision and guidance at refill drinking water depots.   Keywords: Drinking Water, Microbiological Analysis, Refill Drinking Water Depot

scholarly journals Sanitation Practices and Microbial Quality of Drinking Water in Open Defaecation Free and Open Defaecation Communities in the Savelugu Municipality

2021 ◽  
Vol 61 (2) ◽  
pp. 1-12
Author(s):  
N. Bakobie ◽  
A. R. Ibrahim ◽  
A. B. Duwiejuah
Keyword(s):  
Drinking Water ◽  
Microbial Quality ◽  
Salmonella Spp ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
E Coli ◽  
Household Water ◽  
Shigella Spp ◽  
Pit Latrine

Open defaecation leads to faecal matter contamination of drinking water which can cause water borne diseases. The study assessed the linkage between sanitation practices and microbial quality of drinking water of open defaecation free and open defaecation communities in the Savelugu Municipality. A cross-sectional survey of 170 households was conducted in five open defaecation free and five open defaecation communities in the Savelugu Municipality. A total of 78 samples were collected from water sources and households and analysed for Escherichia coli, Salmonella spp and Shigella spp. Sanitation facilities used by the residents included pit latrine (88.24%), no latrine (11.17%), and flush (0.59%). The study observed improper disposal of children faeces in the communities. E. coli count ranged from 0 to 15 CFU/100 ml in open defaecation free communities and 0 to 32 CFU/100 ml in open defaecation communities. The microbial load in the drinking water from open defaecation communities was higher than open defaecation free communities. Open defaecation practice leads to contamination of household water probably due to unsanitary. Community Led Total Sanitation (CLTS) should be encouraged in open defaecation communities.

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