scholarly journals ANALISIS KELIMPAHAN TOTAL BAKTERI COLIFORM DI PERAIRAN MUARA SUNGAI SAYUNG, MOROSARI, DEMAK (Analysis of Total Abundance of Coliform Bacteria at the Sayung River Estuary, Morosari, Demak)

Author(s):  
Lani Febriana Safitri ◽  
Niniek Widyorini ◽  
Oktavianto Eko Jati

Perairan Muara Sungai Sayung terletak di wilayah Kabupaten Demak sebelah barat. Perairan ini telah dipengaruhi oleh berbagai aktivitas manusia yang menyebabkan terjadinya penurunan kualitas perairan. Bakteri Coliform merupakan bakteri gram negatif yang berbentuk batang dan tidak membentuk spora. Bakteri ini merupakan bakteri indikator terjadinya pencemaran perairan maupun indikator keberadaan bakteri patogen lainnya. Perhitungan total bakteri Coliform perlu dilakukan untuk mengetahui adanya pencemaran limbah organik yang terjadi di Perairan Muara Sungai Sayung, Morosari, Demak. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan total bakteri Coliform, dan status perairan di Perairan Muara Sungai Sayung, Morosari, Demak. Penelitian ini juga bertujuan untuk mengetahui hubungan dari jumlah total bakteri Coliform dengan bahan organik terlarut dan oksigen terlarut (DO). Pelaksanaan penelitian ini dilakukan pada bulan Mei 2018. Metode yang digunakan dalam penelitian ini adalah metode survei dan teknik pengambilan sampel menggunakan metode random sampling yang dilakukan pada lima stasiun. Hasil penelitian menunjukkan bahwa kelimpahan rata-rata total bakteri Coliform yang diperoleh berkisar antara 4 x 103 – 550 x 10 3/100ml. Kelimpahan rata-rata total bakteri Coliform yang diperoleh pada setiap stasiun menunjukkan bahwa kandungan total bakteri Coliform telah melebihi baku mutu air menurut Keputusan Menteri Lingkungan Hidup Nomor 51 Tahun 2004. Keberadaan total bakteri Coliform memiliki hubungan yang lemah dengan kandungan bahan organik terlarut, sedangkan keberadaan total bakteri Coliform memiliki hubungan yang cukup dengan oksigen terlarut (DO) di perairan Muara Sungai Sayung Morosari, Demak. Sayung River Estuary is located in the western part of Demak Regency. This estuary has been affected by various human activities which have led to a decline in water quality. Coliform bacteria are a gram-negative bacteria that are rod-shaped and do not form spores. This bacterium is an indicator bacteria of water pollution and an indicator of the presence of other pathogenic bacteria. Total Coliform bacteria calculation needs to be carrid out to find out if there any contamination of organic waste in Sayung River Estuary, Morosari, Demak.. The purpose of this research was to determine total abundance of Coliform bacteria, water quality in Sayung River Estuary, Morosari, Demak and relationship between the total abundance of Coliform bacteria with dissolved organic matter and dissolved oxygen (DO). This research was carried out in May 2018. The method that used in this research were survey method and the sampling technique was random sampling method in five stations. The results showed that the average abundance of total Coliform bacteria obtained ranged between 4 x 103 - 550 x 103 / 100ml. Average abundance of total Coliform bacteria obtained at each station shows that the total content of Coliform bacteria has exceeded the water quality standard according to Minister of Environment Decree number 51 of 2004. The total presence of Coliform bacteria has a weak relationship with the content of dissolved organic matter, while the total number of Coliform bacteria have sufficient relationship with dissolved oxygen (DO) in the Sayung River Estuary,Morosari, Demak.

2020 ◽  
Vol 8 (4) ◽  
pp. 309-315
Author(s):  
Dilia Puspita Asih ◽  
Churun Ain ◽  
Niniek Widyorini

Sungai Banjir Kanal Barat dan Silandak merupakan sungai besar yang berada di Kota Semarang. Terdapat berbagai aktivitas di sepanjang aliran sungai tersebut, diantaranya aktivitas domestik dan industri dimana buangan limbah masuk ke dalam badan sungai sehingga menyebabkan penurunan kualitas perairan. Salah satu mikroorganisme yang terkandung dalam limbah domestik yang berperan sebagai indikator pencemaran yaitu bakteri coliform sehingga perlu dilakukan perhitungan total coliform guna mengetahui adanya pencemaran di Sungai Banjir Kanal Barat dan Silandak. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan total coliform, status mutu air berdasarkan PP Nomor 82 Tahun 2001 kelas II dan hubungan antara total coliform dengan bahan organik dan BOD (Biological Oxygen Demand) di Sungai Banjir Kanal Barat dan Silandak. Metode yang digunakan dalam penelitian ini yaitu metode survei. Pengambilan sampel terdiri dari 2 stasiun (A dan B) yang masing-masing terdapat 5 titik dan 2 kali pengulangan (P1 dan P2). Variabel utama yang dianalisis yaitu total coliform, bahan organik dan BOD, variabel pendukung yang diukur yaitu temperatur, salinitas, DO (Dissolved Oxygen ) dan pH. Berdasarkan hasil penelitian, jumlah total coliform pada stasiun A berkisar antara 240-16000 MPN/100 mldan 23-5400 MPN/100 ml pada stasiun B. Nilai total coliform pada titik A2 dan B2 pada P1 serta A5 P2telah melebihi bakumutu. Hubungan antara total coliform dengan bahan organik yaitu sangat lemah (nilaiPearson Correlation = 0,15). Hubungan antara total coliform denganBOD yaitu lemah (nilaiPearson Correlation= 0,378). Banjir Kanal Barat and Silandak Rivers are large rivers in Semarang. There are various activities along the river flow, including domestic activities and industries where waste effluents enter the river bodies causing a decrease in water quality. One of the microorganisms contained in domestic waste that as an indicator of pollution is coliform bacteria, so it is necessary to calculate the total coliform in order to find out the pollution in the Banjir Kanal Barat and Silandak Rivers. This study aims to determine abundance of total Coliform, water quality status based on PP No. 82 of 2001 class II and relationship between the total coliform with organic matter and BOD (Biological Oxygen Demand) in the Banjir Kanal Barat and Silandak Rivers. The method used in this study is survey method. Sampling consisted of 2 stations (A and B), each of which had 5  points and 2 repetitions (P1 and P2). The main variables analyzed were total coliform, organic matter and BOD, the supporting variable analiyzed were temperature, salinity, DO (Dissolved Oxygen ) and pH. Based on the results of the study, the total number of coliform at station A ranged from 240-16000 MPN/100 ml and 23-5400MPN/100 ml at station B. The total value of coliform at points A2 and B2 at P1 and A5 P2 has exceeded the standard. The relationship between total coliform and organic matter is very weak (Pearson Correlation value = 0.15). The relationship between total coliform and BOD is weak (Pearson Correlation value = 0.378). 


2019 ◽  
Vol 8 (3) ◽  
pp. 211-220
Author(s):  
Avenda Diza Pratiwi ◽  
Niniek Niniek Widyorini ◽  
Arif Rahman

ABSTRAK Sungai Plumbon merupakan sungai yang berada di daerah Mangkang Kulon, terletak di pemukiman padat penduduk. Terdapat sumber pencemar yang masuk ke dalam perairan diantaranya limbah industri rumahan maupun limbah rumah tangga Kegiatan ini dapat menurunkan kualitas perairan. Salah satu cara untuk mengetahui kualitas perairan adalah berdasarkan total bakteri Coliform. Tujuan dari penelitian adalah untuk mengetahui total bakteri Coliform, hubungan total bakteri Coliform dengan nitrat serta bahan organik total dan mengetahui beban pencemaran beserta indeks pencemaran di sungai Plumbon, Semarang. Penelitian ini dilaksanakan pada bulan Maret 2019 dilakukan setiap seminggu sekali dalam waktu dua minggu. Metode yang digunakan pada penelitian adalah metode observasi. Teknik pengambilan sampel menggunakan metode purposive sampling pada 3 stasiun dengan 3 titik di setiap stasiunnya. Analisis data yang digunakan meliputi analisis kepadatan bakteri Coliform, analisis kualitas air berdasarkan baku mutu dan indeks pencemaran, analisis beban pencemaran serta analisis korelasi pearson. Hasil penelitian menunjukan bahwa kepadatan rata-rata total bakteri Coliform berkisar antara 5.566 – 1.203.333 MPN/100 ml. Keberadaan total bakteri Coliform memiliki hubungan yang lemah dengan nitrat dan bahan organik total di Sungai Plumbon, Semarang. Kepadatan rata-rata total bakteri Coliform dan beban pencemaran bakteri Coliform yang diperoleh pada setiap stasiun tidak memenuhi baku mutu kelas II menurut Peraturan Pemerintah No. 82 tahun 2001, sedangkan status pencemaran di sungai Plumbon dikategorikan sebagai tercemar ringan hingga sedang. ABSTRACT Plumbon River is a river in Mangkang Kulon, It is located in densely populated settlements. There are pollutant resource such as home industry waste and household waste in the water. It can decrease water quality. The waters quality can be predicted with the total number of coliform bacteria. This research was aimed to obtain total Coliform bacteria, find the correlation between total Coliform bacteria with nitrate and organic matter, and pollution load with pollution index in Plumbon River, Semarang. This research was conducted on March 2019 and it was held every week in two weeks. The method used in this research was observation method. The sample of this research was taken purposively in 3 stations with 3 points in every station. The data analysis included the analysis of Coliform bacteria density, the analysis of water quality based on quality standards and waters index, the analysis of pollution load, and the analysis of pearson correlation. The result of this research was showed that average density of total Coliform bacteria in the range between 5.566 - 1.203.333 MPN/100 ml. The existence of total Coliform bacteria has low relation with nitrate and total organic matter in Plumbon River, Semarang. The average density of total Coliform bacteria and bacterial pollution load in every station was not fulfill the quality standards class II. Based on Government Regulation No. 82 of 2001,  whereas pollution status of Plumbon River has been categorized as mild to moderate polluted.


Author(s):  
Rizky Muliani Dwi Ujianti ◽  
Althesa Androva

 Abstract. Banjir Kanal Barat is a river in the Garang watershed, Semarang City, Central Java, Indonesia. Its function is as a source of water for the community. The level of pollution in this river is already high. The purpose of this study is to provide advice to governments, communities and related stakeholders to realize integrated river management, and fisheries-based food security is achieved. This research method is: analyzing the water quality of the Banjir Kanal Barat river, and analyzing the amount of faecal and total coliform bacteria content in the Banjir Kanal Barat river, and analyzing how to overcome the decline in the quality of waters of the Banjir Kanal Barat river due to faecal and total coliform bacteria pollution. The results showed that the water quality at the research location was still in the quality standard. The content of coliform dan faecal bacteria at the study site exceeds the quality standard, this is due to the influence of domestic waste from households. The thing that needs to be done is counseling the existence of a clean and healthy life, especially for people who are still throwing domestic waste into the river. The existence of water purification equipment is also very necessary to overcome this problem. Water quality management can be done with policy analysis. Regulations related to water quality management can be analyzed and then given solutions and recommendations related to these rules so that policies can be taken that are sustainable, integrated, and coordinated between various parties in managing river water quality and food security. Keywords: food security, water quality, river, faecal coliform, total coliform


2015 ◽  
Vol 10 (1) ◽  
pp. 84-90
Author(s):  
E Fadairo ◽  
M Otite-Douglas

This study investigated the effects of boiling temperature and associated physico-chemical parameters on the Petroleum Training Institute potable water and the possibility of biofilm formation in its delivery systems. A total of 25 potable water samples were used for this study. The environmental parameters investigated were pH, conductivity, total dissolved solids (TDS), total suspended solid (TSS), dissolved oxygen (DO1), / DO5, salinity, resistivity, total coliform bacteria (as an indicator of possible biofilm presence in the distribution system) and biofilm . An overall prevalence of <1 of the total coliform bacteria was observed in the plus-boiling and minus-boiling potable water sample, except for the female hostel which showed moderate stain for the qualitative biofilm test. For the minus-boiling water sample, pH values were between 5.04±0.47 to 6.82±0.48; Total suspended solids ranged between 0.09±0.05-0.17±0.02, total dissolved solid ranged between 4.07±0.73 to 5.58±0.70, conductivity values ranged between 8.02±0.90 to 11.54±1.67, dissolved oxygen ranged between 1.97±0.26 to 3.12 ±0.13, the DO5 ranged between 1.91±0.32 to 2.72± 0.29 while resistivity ranged between 7.79±0.13 to 10.88±0.18. Values for the Plus-boiling and filtered samples showed a pH range of 6.02±0.26 to 6.95±0.26; conductivity 7.21±0.10 to 9.88±0.67; DO ranged between 1.01±0.14 to 2.08±0.35, DO 5 was 1.02±0.02 to 2.01±0.38, TSS and TDS ranged between 0.02±0.001, 3.74±0.62 to 0.03±0.002 and 4.95±0.42 respectively while resistivity ranged between 1.02±0.11 to 1.98±0.16. For all parameters analyzed, values obtained falls within the WHO limit for potable water except for the qualitative biofilm test on FSH minus-boiling water sample which gave moderate stain with 0.1% crystal violet stain and the pH values which fall below WHO acceptable limits. Boiling and filtration of potable water irrespective of the source is campaigned from this study in order to enhance the prevention and management of water borne diseases within the institute community.


2020 ◽  
Vol 10 (2) ◽  
pp. 298-308
Author(s):  
Carlos I. Gonzalez ◽  
John Erickson ◽  
Karina A. Chavarría ◽  
Kara L. Nelson ◽  
Amador Goodridge

Abstract Safe water storage is critical to preserve water quality, especially when intermittent piped drinking water supply creates a need for household storage. This study characterized household storage practices and stored water quality in 94 households (N = 94) among four peri-urban neighborhoods in Arraiján, Panama with varying degrees of supply intermittency. We found that 18 (19.1%) households stored drinking water in unsafe containers. Forty-four (47%) samples of household stored drinking water had residual chlorine levels &lt;0.2 mg/L. While 33 (35.1%) samples were positive for total coliform bacteria, only 23 (24.4%) had &gt;10 most probable number (MPN)/100 mL total coliform bacteria. Eight (44%) samples were positive for Escherichia coli, whereas only one (1.3%) sample from the safe containers was positive. Twenty-nine (30.9%) samples had &gt;500 MPN/mL heterotrophic plate count bacteria. These findings suggest that longer supply interruptions were associated with longer storage times and lower chlorine residual, which were associated with higher concentrations of indicator bacteria. This is one of the first studies in the Central-American region to show an association between the lack of turnover (replacement with fresh water) and greater contamination during household water storage. Thus, when drinking water supply is not completely continuous and household storage is required, decreasing the time between supply periods can facilitate safer water storage. Public awareness and education are also recommended to increase hygiene practices during water collection and storage.


Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 670 ◽  
Author(s):  
Shatha Alazzeh ◽  
Stephanie Galaitsi ◽  
Amahl Bishara ◽  
Nidal Al-Azraq ◽  
John L. Durant

We investigated the quality of treated drinking water in Aida Camp and Alazzah Camp, two Palestinian refugee camps in the West Bank. Water supply in the two camps is intermittent: Water deliveries are first stored in a centrally located community tank before being pumped through distribution networks to users, who then store the water in rooftop tanks. Between March 2016 and June 2017, we examined water quality within the distribution system in the camps, testing 520 samples in Aida Camp and 198 samples in Alazzah Camp for total coliforms, Escherichia coli (E. coli) bacteria, and residual chlorine. We observed deterioration in water quality in both camps, but the deterioration was more pronounced in Aida Camp where 2.5% of community-tank samples, 5.0% of network samples, and 10% of rooftop-tank samples contained one or more colonies (per 100 mL) of total coliform bacteria. In Alazzah Camp, 3.7% of community-tank samples, 2.5% of network samples, and 5% rooftop-tank samples contained one or more colonies (per 100 mL) of total coliform bacteria. E. coli was detected in two samples from Aida Camp and one sample from Alazzah Camp. In both camps, average residual chlorine concentrations were higher in the community tanks than in rooftop tanks. The main factor influencing water quality deterioration in rooftop tanks in Aida Camp appears to be air temperature. We recommend more systematic water testing in the camps and greater transparency in reporting test results to camp residents.


2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi


1970 ◽  
Vol 24 (2) ◽  
pp. 163-165 ◽  
Author(s):  
Abdul Hussain Shar ◽  
Yasmeen F Kazi ◽  
Miandad Zardari ◽  
Irshad Hussain Soomro

Total coliform (TC) and faecal coliform (FC) bacteria were analyzed in drinking water of Khairpur city. Ninty samples were collected from main reservoir (source), distribution line and consumer taps. pH and residual chlorine of water samples were also determined. For bacteriological analysis inductively membrane filtration (MF) method was used for total coliform (TC) as well as faecal (FC) coliform bacteria. All samples were found contaminated with total coliform (TC) and faecal coliform (FC) and the counts were higher than the maximum microbial contaminant level (MMCL) established by World Health Organization (WHO). It was observed that pH was within the limits of WHO standard (6.5-8.5). The residual chlorine was not detected in any sample of drinking water. Bacteriologically the water quality of the drinking water is unsatisfactory.Keywords: Coliform, Escherichia coli, Water quality, Contamination, SanitationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1266


2021 ◽  
Vol 880 (1) ◽  
pp. 012039
Author(s):  
N U M Nizam ◽  
M M Hanafiah ◽  
M B Mokhtar ◽  
N A Jalal

Abstract Prolonged drought, population growth and water demand for various purposes have increased the water scarcity issue. To overcome this issue, a rainwater harvesting system can be utilized as an alternative for clean water supply. A rainwater harvesting system is a method of collecting rainwater from man-made surfaces such as rooftops and constructed surfaces and can be used for various sectors including household, agricultural and commercial. This study was conducted to determine the quality of rainwater harvested collected directly from rooftop. The quality of the rooftop rainwater was taken in three consecutive months and the water quality for before and after treatment was measured and compared. Commercial activated carbon was used to treat the rainwater obtained from the rooftop. The water quality was compared with the Water Quality Index (WQI) and the National Water Quality Standards (NWQS). The parameters involved are pH, temperature, conductivity, dissolved oxygen (DO), total suspended solids (TSS), ammoniacal nitrogen (NH3-N), biochemical oxygen demand (BOD), chemical oxygen demand (COD),E.coli and total coliform bacteria. The results showed that the total value of WQI before and after treatment was 86.3 ± 8.963 and 87.6±2.081, respectively. Positive correlations were found for parameter NH3-N, COD and pH, while paired T-test showed a significant in the COD and the presence of bacteria. Total Coliform is still at a safe level by NWQS with the average value and the standard deviation for before and after treatment were 38.11 ± 13.960 cfu/ml and 10.33 ± 6.671 cfu/ml, respectively.


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