scholarly journals Hubungan Higiene, Fasilitas dan Sanitasi Lingkungan dengan Kualitas Mikrobilogi Serta Identifikasi Eschericia Coli O157: H7 Pada Sate Languan

2019 ◽  
Vol 18 (2) ◽  
pp. 104
Author(s):  
Sang Gede Purnama ◽  
Made Subrata
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Total Sample ◽  
Raw Material ◽  
Food Handler ◽  
Environmental Sanitation ◽  
E Coli ◽  
Sequential Explanatory Design ◽  
Sequential Explanatory ◽  
Surrounding Areas

Latar belakang: Proses pembuatan sate languan yang dari bahan baku ikan rentan mengalami kontaminasi secara mikrobiologi akibat kontaminasi alat luluh yang kurang dibersihkan dan penjamah makanan. Oleh sebab itu, perlu dilakukan identifikasi cemaran dan faktor risikonya.Metode: penelitian ini menggunakan metode campuran (mixed methode) dengan rancangan penelitian sequential explanatory design yakni menggabungkan dua bentuk penelitian yakni kuantitatif dan kualitatif dimana pada tahap pertama mengambil data kuantitatif selanjutnya data kualitatif. Pendekatan kuantitatif juga dilakukan dengan observasi secara langsung oleh peneliti.  Jumlah sampel seluruh pedagang Sate Languan di Pantai Lebih dan sekitarnya sebanyak 19 rumah makan dan 19 sampel Sate Languan.  Variabel yang diteliti higiene penjamah makanan, kepemilikan alat luluh, ketersediaan fasilitas sanitasi,  sanitasi lingkungan, lama kerja dan pendidikan. Pemeriksaan mikrobiologi dengan identifikasi Eschericia coli O157:H7 dengan pembiakan pada media eosin methylene blue agar (EMBA), identifikasi  E. coli O157:H7 dilanjutkan dengan penumbuhan isolate bakteri di media selektif sorbitol MacConkey agar (SMAC) dilanjutkan dengan uji konfirmasi dengan lateks O157.Hasil: variabel yang berhubungan dengan kontaminasi Eschericia colipada sate languan yakni higiene penjamah makanan, sarana fasilitas sanitasi dan kepemilikan alat luluh. Higiene penjamah makanan yang termasuk kategori kurang baik sebanyak (79%), kategori fasilitas sanitasi tidak memadai sebanyak (53%), kategori sanitasi lingkungan kurang bersih sebanyak (47%). Dari 19 sampel yang dilakukan pemeriksaan coliform dan E. coli diketahui 15 warung makanterkontaminasi E. coli rata-rata 5 x 106 cfu/gram hanya  4 warung yang E. coli masih dalam batas aman. Hasil uji lanjutan diketahui bahwa negatif Eschericia coli O157:H7.Simpulan : ada hubungan higiene penjamah makanan, sarana fasilitas sanitasi dan kepemilikan alat luluhdengan kontaminasi E. coli. Ditemukan cemaran E. coli pada makanan Sate Languan namun tidak terbukti jenis E. coliO157:H7. ABSTRACTTitle: The Relationship of Hygiene, Facilities and Environmental Sanitation with The Identification of Eschericia Coli O157: H7 at Languan Satay.Background: The process of processing satay from raw material from chopped fish is susceptible to microbiological contamination due to unclean contamination of equipment and food handlers. Therefore, it is necessary to identify contaminants and risk factors.Method: This study uses (mixed method) a sequential explanatory design that combines two forms of research, namely quantitative and qualitative, where in the first stage, the quantitative data is then taken from qualitative data. The quantitative approach is also carried out by direct observation by the researcher. The total sample of all satay traders in Lebih Beach and surrounding areas are 19 restaurants and 19 samples of Languan Satay. The variables studied were food handler hygiene, ownership of meat crusher, availability of sanitation facilities, environmental sanitation, length of work and education. Microbiological examination with identification of Escherichia coli O157: H7 with culture on eosin methylene blue agar (EMBA) media, identification of E. coli O157: H7 followed by growth of bacterial isolates in selective Sorbitol Mac Conkey agar (SMAC) followed by confirmation test with O157 latex.Results: variables related to Escherichia coli contamination in satay is food handler hygiene, sanitation facilities and ownership of meat crusher. Food handler hygiene included in the poor category (79%), inadequate sanitation facilities category (53%) and less clean environmental sanitation category (47%). Of the 19 samples that were examined for coliform and E. coli, it was found that 15 stalls contaminated with E. coli averaged 5 x 106 cfu / gram, only 4 stalls which were still within safe limits. The results of the follow-up test revealed that negative Eschericia coli O157: H7.Conclusion:There is a relationship between food handler hygiene, sanitation facilities and ownership of tool with E. coli contamination.It was found E. coli contamination in Languan Satay but it was not proven to be E. coli O157: H7. 

scholarly journals Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material

2020 ◽  
Vol 47 (12) ◽  
pp. 1117-1132
Author(s):  
Katharina Novak ◽  
Juliane Baar ◽  
Philipp Freitag ◽  
Stefan Pflügl
Keyword(s):  
Escherichia Coli ◽  
Cheese Whey ◽  
Strain Improvement ◽  
Defined Medium ◽  
Raw Material ◽  
Efficient Production ◽  
Substrate Uptake ◽  
Chemically Defined Medium ◽  
E Coli ◽  
Plasmid Library

AbstractThe aim of this study was to establish isobutanol production on chemically defined medium in Escherichia coli. By individually expressing each gene of the pathway, we constructed a plasmid library for isobutanol production. Strain screening on chemically defined medium showed successful production in the robust E. coli W strain, and expression vector IB 4 was selected as the most promising construct due to its high isobutanol yields and efficient substrate uptake. The investigation of different aeration strategies in combination with strain improvement and the implementation of a pulsed fed-batch were key for the development of an efficient production process. E. coli W ΔldhA ΔadhE Δpta ΔfrdA enabled aerobic isobutanol production at 38% of the theoretical maximum. Use of cheese whey as raw material resulted in longer process stability, which allowed production of 20 g l−1 isobutanol. Demonstrating isobutanol production on both chemically defined medium and a residual waste stream, this study provides valuable information for further development of industrially relevant isobutanol production processes.

scholarly journals Removal of Methylene blue, Escherichia coli and Pseudomonus aeruginosa by Adsorption Process of Activated Carbon Produced from Moringa oleifera Bark

2021 ◽  
Author(s):  
Md. Shahin Azad ◽  
Syaza Azhari ◽  
Mohd Sukri Hassan
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Activated Carbon ◽  
Moringa Oleifera ◽  
Adsorption Process ◽  
Diffusion Mechanism ◽  
Bacterial Load ◽  
Batch Adsorption ◽  
Maximum Adsorption Capacity ◽  
E Coli

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.

Comparison of the β-Glucuronidase Assay and the Conventional Method for Identification of Escherichia coli on Eosin-Methylene Blue Agar

1997 ◽  
Vol 60 (1) ◽  
pp. 6-9 ◽  
Author(s):  
SHIU W. HUANG ◽  
CHUNG H. CHANG ◽  
TUNG F. TAI ◽  
TSUNG C. CHANG
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Conventional Method ◽  
Meat Products ◽  
Mixed Cultures ◽  
Effective Alternative ◽  
Methyl Red ◽  
Raw Meat ◽  
E Coli ◽  
Glucuronidase Assay

The purpose of this study was to compare the IMViC (indole, methyl red, Voges-Proskauer and citrate utilization) tests with the β-glucuronidase (GUD) assay for the identification of suspect Escherichia coli on Levine's eosin-methylene blue (EMB) agar. After testing 258 suspect E. coli colonies from raw meat and meat products, 163 and 44 were found to be E. coli and non-E. coli, respectively, by both methods. Nine isolates were IMViC positive (i.e., + + − − or − + − −) but GUD negative; among these isolates, six were confirmed to be E. coli by API 20E (bioMérieux, Marcy-I'Etoile, France) with the remaining three being non-E. coli. There were 42 isolates that were IMViC negative but GUD positive; among these isolates, seven were pure E. coli cultures, 33 were mixed cultures containing E. coli, and the remaining two were Proteus spp. The sensitivities for the identification of E. coli on EMB were 80.9% (169/209) and 97.1% (203/209), respectively, by the IMViC tests and GUD assay; whereas the specificities were 93.9% (46/49) and 95.9% (47/49), respectively, by the IMViC tests and GUD assay. It is proposed that the GUD assay can be an effective alternative to the conventional IMViC tests for the identification of suspect E. coli on EMB.

Differently ordered carbonaceous structures synthesized by bubbled Ar or He plasmas inside methylene blue solutions with contrasting Escherichia coli growth inhibition effects

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98325-98334 ◽  
Author(s):  
D. Zaharie-Butucel ◽  
J. Papp ◽  
C. Leordean ◽  
S. D. Anghel
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Growth Inhibition ◽  
E Coli ◽  
Structure Synthesis ◽  
Coli Growth

A straightforward carbonaceous structure synthesis from methylene blue aided by Ar/He plasmas with different ordering and E. coli inhibition effects.

scholarly journals Isolation of Shiga toxin-producing strains of Escherichia coli from beef and swine carcasses and the characterization of their genes

2018 ◽  
Vol 9 (2) ◽  
pp. 275-780 ◽  
Author(s):  
O. М. Berhilevych ◽  
V. V. Kasianchuk ◽  
O. M. Deriabin ◽  
M. D. Kukhtyn
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Raw Material ◽  
Animal Origin ◽  
E Coli ◽  
Food Of Animal Origin ◽  
Beef Carcasses ◽  
Positive Isolate ◽  
Stx1 Gene ◽  
Beef Carcass

Escherichia coli is part of the normal microflora of the intestinal tract of humans and warm-blooded animals, but its presence in raw material and food of animal origin is considered as fecal contamination and can be very dangerous for consumers. The determination of the number of E. coli in raw material and food is important because among them can be pathogenic strains. The most dangerous strains are considered enterohemorrhagic E. coli as a causative agent of severe bloody diarrhea and hemorrhagic uremic syndrome in humans through the production of Shiga-toxin, which is the main virulence factor, responsible for disease. The aim of this study was to identify the prevalence of Shiga toxin-producing strains of E. coli (STEC) from swabs of beef and swine carcass in slaughterhouses in Ukraine and characterize their genes, which are responsible for pathogenic properties. A total of 230 samples of swabs from beef (130) and swine (100) carcasses were obtained from 5 slaughterhouses in Ukraine between 2012 and 2015. Samples of swabs from carcasses were randomly selected at the final point of the process after the final washing of the carcass from the following areas: distal hind limb, abdomen (lateral and medial) from swine carcasses, brisket, flank and flank groin areas from beef carcasses. All samples were examined by culture-dependent method, after that each positive isolate of STEC was analyzed by multiplex PCR to detect the stx1, stx2, and eae genes. Out of 230 collected samples, seven (7.2%) were contaminated with STEC. The highest prevalence of STEC was found in swabs from beef carcasses (8.1%) in comparison to swabs from swine carcasses (5.7%). The stx1 gene was the predominant gene detected in all STEC positive samples. The eae gene was found in one of the examined isolates from beef carcass. Three isolates from swabs of beef carcass carried both stx1 and stx2 genes, one isolate showed association between stx1 and eae genes, one isolate was positive for stx1 gene only. In swabs from swine carcasses (2 isolates) stx1 and stx2 genes were presented simultaneously. The results of this study suggested that fresh raw meat could be a potential vehicle for transmission of the Shiga toxin-producing strain of E. coli to humans. This is the first report of STEC prevalence in beef and swine carcasses in Ukraine and these data will be valuable for microbiological risk assessment and help the appropriate services to develop strategies to mitigate health risk.

scholarly journals Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

2020 ◽  
Vol 10 (2) ◽  
pp. 106
Author(s):  
Dian Meididewi Nuraini ◽  
Morsid Andityas ◽  
Adi Paramarta ◽  
Nur Rohman Najib ◽  
Agustina Dwi Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Drinking Water ◽  
Inhibitory Activity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Test ◽  
Aloe Barbadensis ◽  
E Coli

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%.  Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity   Abstrak  Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%.  Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

scholarly journals Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi

2021 ◽  
Vol 4 (2) ◽  
pp. 207
Author(s):  
Achmad Arby Wijaya ◽  
Iwan Sahrial Hamid ◽  
Maya Nurwartanti Yunita ◽  
Wiwiek Tyasningsih ◽  
Ratih Novita Praja
Keyword(s):  
Escherichia Coli ◽  
Total Sample ◽  
Cow Milk ◽  
Most Probable Number ◽  
Methyl Red ◽  
Probable Number ◽  
E Coli ◽  
Fresh Milk ◽  
Peptone Water ◽  
Mpn Test

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.

scholarly journals Evaluation of the Sanitary Conditions of Head Meat, Esophagus, Diaphragm Meat, and Boning Scrap Processing

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Elis Caroline Celestina dos Santos ◽  
Adelino Cunha Neto ◽  
Vinicius Silva Castro ◽  
Ricardo Cesar Tavares Carvalho ◽  
Eduardo Eustáquio de Souza Figueiredo
Keyword(s):  
Escherichia Coli ◽  
European Union ◽  
Raw Material ◽  
Microbiological Contamination ◽  
Total Coliforms ◽  
E Coli ◽  
End Products ◽  
Sanitary Conditions ◽  
Hygienic Conditions

Enterobacteriaceae, coliforms, and Escherichia coli counts are important hygiene indicators and may be pathogenic. Thus, the aim of the present study was to determine microbiological contamination in head meat, the esophagus, diaphragm, and boning scraps and evaluate the hygienic conditions of the processing of these products. The Petrifilm® (3M) method for determining Enterobacteriaceae, total coliforms, and E. coli was applied for 104 samples. APHA, European Union, PAHO/WHO, and Brazil/MAPA recommendations were followed. Bleeding and skinning knives were contaminated with E. coli (61.5%). Regarding the meat cuts, 30.76% samples from head meat, the esophagus and the boning flap showed the presence of E. coli in counts up to 2 log CFU/g, while 15.3% of the diaphragm samples showed up to 1.85 log CFU/g. The analyzed comminuted meat was, therefore, shown to be contaminated with E. coli during processing, indicating that end-products from this raw material can offer biological risks.

Isolation and partial characterization of an Escherichia coli mutant resistant to colicin A

1975 ◽  
Vol 21 (10) ◽  
pp. 1595-1601 ◽  
Author(s):  
Marc Lavoie ◽  
Leo G. Mathieu
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Parent Strain ◽  
General Pattern ◽  
Sodium Dodecyl ◽  
Triphenyltetrazolium Chloride ◽  
E Coli ◽  
Receptor Sites ◽  
Acridine Dyes

An Escherichia coli K12 mutant resistant to colicin A-CA31 apparently through loss of its receptor sites has been isolated and partially characterized. Resistance to colicin A was accompanied with a decreased sensitivity to colicins L-398 and E2-CA42, and to acridine dyes. The mutant strain displayed the same general pattern of tolerance or sensitivity as the parent strain towards eight antibiotics, colicins C, D, E1, E3, F2, F3, G, I, K, and N; phages T1, T2, T5, T6, T7, F2, λ vir, P1kc, [Formula: see text] 80, and BF23; and to methylene blue, triphenyltetrazolium chloride, ethylene-diaminetetraacetate (EDTA), deoxycholate, and sodium dodecyl sulfate. Conjugation and transduction experiments showed that a locus controlling resistance to colicin A-CA31 mapped at 21 min on the genetic map of this E. coli K12 strain.

scholarly journals IDENTIFIKASI Escherichia coli O157:H7 PADA SUSU SAPI PERAH DAN LINGKUNGAN PETERNAKAN

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Joshua Liem Tiong Gie ◽  
Yatri Drastini
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Brilliant Green ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cooling Unit

Tujuan penelitian adalah mengidentifikasi Eschericia coli (E. coli) O157:H7 pada susu dan lingkungan peternakan sapi perah. Sampel berjumlah 77 yang terdiri atas 27 sampel susu dan 50 sampel lingkungan. Sampel susu berasal dari ambing sapi (14), milk can peternak (6), milk can tempat penampungan susu (4), dan cooling unit di koperasi (3). Sampel lingkungan berupa feses (14), air sumber dan air tandon (12), pakan (6), serta swab tangan sebelum dan sesudah pemberian minyak pelicin (17), dan tanah (1). Isolasi E. coli dari sampel menggunakan media pemerkaya kaldu brilliant green lactose bile Broth (BGLB), media selektif agar eosin methylene blue (EMB), dan agar sorbitol MacConkey (SMAC). Koloni bakteri yang tidak memfermentasi sorbitol pada SMAC (colorless) diidentifikasi dengan uji aglutinasi lateks O157 dan antisera H7. Identifikasi bakteri dari sampel susu menunjukkan 7,41% (2/27) sampel teridentifikasi E. coli O157. Susu tersebut berasal dari ambing sapi dan milk can peternak. Bakteri E. coli O157 yang teridentifikasi dari sampel lingkungan (sampel pakan) sebanyak 2% (1/50). Hasil uji aglutinasi antisera terhadap tiga sampel positif O157 menunjukkan bahwa ketiganya tidak memiliki antigen H7 dan disimpulkan bahwa tidak ada sampelsusu dan lingkungan yang tercemar E. coli O157:H7.

Sign in / Sign up

Export Citation Format

Share Document