Screening for antibiotic residues in swine and poultry tissues using the STAR test

Majority of the people in Bangladesh are still not aware about the health hazards of antibiotic residues. In this perspective, a survey was undertaken at five different poultry farms in the Dinajpur district. One hundred liver, one hundred thigh muscle and one hundred breast muscle samples were collected from different farms & market places and evaluated by TLC method. A very negligible number of samples were found positive for antibiotics residues. Out of one hundred liver, one hundred thigh muscle and one hundred breast muscle samples respectively; only two liver, two breast muscle and two thigh muscle samples were found positive for amoxicillin antibiotic; five liver, three breast muscle and three thigh muscle samples were found positive for ciprofloxacin; three liver, two breast muscle and two thigh muscle samples were found positive for cephalexin; two liver, two breast muscle and two thigh muscle samples were found positive for enrofloxacin; four liver, two breast muscle and two thigh muscle samples were found positive for oxytetracycline. Gentamicin and neomycin were found negative for any samples. Further investigation was done in indoor discriminate and indiscriminate use of antibiotics (ciprofloxacin and oxytetracycline) in broilers. Liver, thigh muscle and breast muscle samples were collected and evaluated by TLC method. Therefore, poultry treated with antibiotics are required for specific withdrawal period until all residues are depleted to safe levels before human consumption. This research project provides idea that Bangladeshi poultry products are free of antibiotics residues in north area (Dinajpur District) of Bangladesh.

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Antibiotic Residues in Edible Poultry Tissues and Products in Nigeria: A Potential Public Health Hazard

International Journal of Animal and Veterinary Advances ◽

10.19026/ijava.7.5241 ◽

2015 ◽

Vol 7 (3) ◽

pp. 55-61 ◽

Cited By ~ 7

Author(s):

Jallailudeen Rabana Lawal ◽

Saleh Mohammed Jajere ◽

Yaqub Ahmed Geidam ◽

Amina Mohammed Bello ◽

Yakaka Wakil ◽

...

Keyword(s):

Public Health ◽

Health Hazard ◽

Antibiotic Residues ◽

Poultry Tissues ◽

Potential Public Health

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Solid-phase extraction and high performance liquid chromatography with diode array detection method for the determination of antibiotic residues in poultry tissues

Chemical Data Collections ◽

10.1016/j.cdc.2019.100312 ◽

2020 ◽

Vol 25 ◽

pp. 100312 ◽

Cited By ~ 1

Author(s):

Abdulrasaq O. Oyedeji ◽

Titus A.M. Msagati ◽

Akan B. Williams ◽

Nsikak U. Benson

Keyword(s):

High Performance ◽

Detection Method ◽

Solid Phase ◽

Diode Array ◽

Diode Array Detection ◽

Phase Extraction ◽

Antibiotic Residues ◽

Array Detection ◽

Poultry Tissues

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Thin layer chromatographic detection of enrofloxacin antibiotic residues in poultry tissues

Asian-Australasian Journal of Food Safety and Security ◽

10.3329/aajfss.v5i1.55013 ◽

2021 ◽

Vol 5 (1) ◽

pp. 11-18

Author(s):

Md Mominul Islam Bhuiyan ◽

Md Shafiqul Islam ◽

Md Rakibul Hasan ◽

Kazi Rafiqul Islam

Keyword(s):

Body Weight ◽

Thigh Muscle ◽

Control Group ◽

Antibiotic Residues ◽

Withdrawal Period ◽

Antibiotic Group ◽

Group A ◽

Group B ◽

Poultry Tissues ◽

Control Group Group

Antibiotic residues remain in edible portion of meat animals that have been treated with antibiotics. The aim of this study was to detect enrofloxacin residue after discriminate and indiscriminate administration and investigate the effect of enrofloxacin in growth of poultry. 18 broilers DOC (Cobb-500) were collected & reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely Group–A (Control group), Group-B (Discriminate group) and Group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate groups were treated with antibiotic, enrofloxacin. In Group-B withdrawal period was followed and treatment was stopped before 7 days of sacrifice. On the other hand, withdrawal period was not maintained in indiscriminate group and antibiotic treatment was continued until the day of sacrifice. Body weight was recorded daily in the morning. On 31st day mean body weight was highest in Group-C (1901.17 ± 15.22gm) and lowest body weight was in Group-A (1453.33 ± 26.39gm). The differences among mean weight gain were statistically significant (P<0.005) in both discriminate & indiscriminate group compared to control group. Test results found in TLC showed that in discriminate antibiotic group (Group-B) 50% liver samples, 33.33% kidney and 16.67% fat samples were enrofloxacin positive. No sample of thigh muscle, breast muscle and spleen was positive. In indiscriminate antibiotic group (group-C) all the samples were positive in case of liver, kidney, fat and spleen samples. Only 33.33% and 16.67 % samples were positive in case of fat and thigh muscle samples. All the samples of control group (Group-A) were negative. Overall, the present study documented the widespread abuse of enrofloxacin and failure to implement the recommended withdrawal period will undeniably leads to deposition of residues in broiler tissues. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 11-18

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A New Screening Method for the Detection of Antibiotic Residues in Meat and Poultry Tissues

Journal of Food Protection ◽

10.4315/0362-028x-44.11.828 ◽

1981 ◽

Vol 44 (11) ◽

pp. 828-831 ◽

Cited By ~ 28

Author(s):

RALPH W. JOHNSTON ◽

RICHARD H. REAMER ◽

ELMER W. HARRIS ◽

HAROLD G. FUGATE ◽

BERNARD SCHWAB

Keyword(s):

Bacillus Subtilis ◽

Seed Layer ◽

Screening Method ◽

Antibiotic Residues ◽

Cotton Swab ◽

Test Plate ◽

Bacillus Subtilis Atcc ◽

Poultry Tissues ◽

Simplified Procedure ◽

Tissue Fluids

A simplified procedure is described for screening meat and poultry tissues for the presence of antibiotic residues. The method involved inserting a cotton swab directly into meat or poultry tissues, allowing it to absorb tissue fluids. The swab is then removed and placed on a test plate using Antibiotic Medium No.5 (BBL) and a seed layer of Bacillus subtilis ATCC 6633 spores. The plate is incubated overnight at 29 C, then observed for evidence of inhibition around the swab. The method was compared with the conventional bioassay procedures using routing meat and poultry tissues submitted for analysis. Of a total 1,780 tissues tested, the screening procedure was either in agreement with or detected inhibition was not found by the conventional procedures in 99.4% of the samples. The test was shown to have equal sensitivity to conventional procedures for detection of chlortetracycline, oxytetracycline, tetracycline, erythromycin, neomycin, penicillin, streptomycin and tylosin.

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