scholarly journals Thin layer chromatographic investigation of antibiotics residues in edible poultry tissues in Bangladesh

2021 ◽  
Vol 5 (3) ◽  
pp. 024-032
Author(s):  
Md. Shafiqul Islam ◽  
Md. Rakibul Hasan ◽  
Md. Shakil Islam
Keyword(s):  
Breast Muscle ◽  
Thigh Muscle ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Withdrawal Period ◽  
Poultry Products ◽  
The People ◽  
Poultry Farms ◽  
Antibiotics Residues ◽  
Poultry Tissues

Majority of the people in Bangladesh are still not aware about the health hazards of antibiotic residues. In this perspective, a survey was undertaken at five different poultry farms in the Dinajpur district. One hundred liver, one hundred thigh muscle and one hundred breast muscle samples were collected from different farms & market places and evaluated by TLC method. A very negligible number of samples were found positive for antibiotics residues. Out of one hundred liver, one hundred thigh muscle and one hundred breast muscle samples respectively; only two liver, two breast muscle and two thigh muscle samples were found positive for amoxicillin antibiotic; five liver, three breast muscle and three thigh muscle samples were found positive for ciprofloxacin; three liver, two breast muscle and two thigh muscle samples were found positive for cephalexin; two liver, two breast muscle and two thigh muscle samples were found positive for enrofloxacin; four liver, two breast muscle and two thigh muscle samples were found positive for oxytetracycline. Gentamicin and neomycin were found negative for any samples. Further investigation was done in indoor discriminate and indiscriminate use of antibiotics (ciprofloxacin and oxytetracycline) in broilers. Liver, thigh muscle and breast muscle samples were collected and evaluated by TLC method. Therefore, poultry treated with antibiotics are required for specific withdrawal period until all residues are depleted to safe levels before human consumption. This research project provides idea that Bangladeshi poultry products are free of antibiotics residues in north area (Dinajpur District) of Bangladesh.

scholarly journals Thin layer chromatographic detection of enrofloxacin antibiotic residues in poultry tissues

2021 ◽  
Vol 5 (1) ◽  
pp. 11-18
Author(s):  
Md Mominul Islam Bhuiyan ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Body Weight ◽  
Thigh Muscle ◽  
Control Group ◽  
Antibiotic Residues ◽  
Withdrawal Period ◽  
Antibiotic Group ◽  
Group A ◽  
Group B ◽  
Poultry Tissues ◽  
Control Group Group

Antibiotic residues remain in edible portion of meat animals that have been treated with antibiotics. The aim of this study was to detect enrofloxacin residue after discriminate and indiscriminate administration and investigate the effect of enrofloxacin in growth of poultry. 18 broilers DOC (Cobb-500) were collected & reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely Group–A (Control group), Group-B (Discriminate group) and Group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate groups were treated with antibiotic, enrofloxacin. In Group-B withdrawal period was followed and treatment was stopped before 7 days of sacrifice. On the other hand, withdrawal period was not maintained in indiscriminate group and antibiotic treatment was continued until the day of sacrifice. Body weight was recorded daily in the morning. On 31st day mean body weight was highest in Group-C (1901.17 ± 15.22gm) and lowest body weight was in Group-A (1453.33 ± 26.39gm). The differences among mean weight gain were statistically significant (P<0.005) in both discriminate & indiscriminate group compared to control group. Test results found in TLC showed that in discriminate antibiotic group (Group-B) 50% liver samples, 33.33% kidney and 16.67% fat samples were enrofloxacin positive. No sample of thigh muscle, breast muscle and spleen was positive. In indiscriminate antibiotic group (group-C) all the samples were positive in case of liver, kidney, fat and spleen samples. Only 33.33% and 16.67 % samples were positive in case of fat and thigh muscle samples. All the samples of control group (Group-A) were negative. Overall, the present study documented the widespread abuse of enrofloxacin and failure to implement the recommended withdrawal period will undeniably leads to deposition of residues in broiler tissues. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 11-18

scholarly journals Thin layer chromatographic detection of colistin sulfate antibiotic residues in poultry tissues

2021 ◽  
Vol 7 (1) ◽  
pp. 01-08
Author(s):  
Md. Nazmul Hasan ◽  
Md. Shafiqul Islam ◽  
Md. Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Thin Layer ◽  
The Body ◽  
Control Group ◽  
Human Consumption ◽  
Broiler Chicks ◽  
Antibiotic Residues ◽  
Withdrawal Period ◽  
Liver Sample ◽  
Colistin Sulfate ◽  
Antibiotic Group

Residues of antibiotic drugs in food and food products have been received much attention in recent years because of growing food safety concerns for public health. There are serious effects of antibiotic residues in meat for human consumption (e.g., increasing antimicrobial resistance, carcinogenicity, mutagenicity, and hypersensitivity). In this study, we used broiler chicks as a laboratory animal to detect the colistin sulfate antibiotic residue for human health concerns. The day-old broiler chicks were collected and reared for up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with an antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The body weights of the birds were recorded daily. The mean body weight was highest in indiscriminate group (1261.15±16.37gm) followed by discriminate group (1156.15±18.23gm) and the lowest was in control group (1008.49±18.11gm). The differences among mean weight gain were statistically significant (P<0.05) in antibiotic treated group compared with control group. The Thin Layer Chromatography revealed that all the samples were positive in indiscriminate group and in case of Liver, Kidney and Spleen, it was 100%. In case of discriminate antibiotic group all the samples were positive except thigh and breast muscle and the percentage was highest in Liver sample (66.67%). There was no positive sample in control group. The results were statistically significant (P<0.05). From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic abuse in poultry industry.

scholarly journals Prevalence of Salmonella in dressed and cooked broiler meat of different grocery shops, hotels and restaurants in Gazipur and Dhaka city of Bangladesh

2018 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
AKM Ziaul Haque ◽  
SM Lutful Kabir ◽  
Ayesha Siddiqua ◽  
AZM Iftikhar Hussain
Keyword(s):  
Fast Food ◽  
Breast Muscle ◽  
Thigh Muscle ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Broiler Meat ◽  
Poultry Products ◽  
Thigh Muscles ◽  
Cooked Meat

The study was conducted to find out the prevalence of Salmonella in dressed and cooked broiler meat in different shops and restaurants in Gazipur and Dhaka city including the sanitary quality. A total of 80 samples of dressed boiler, 88 cooked samples from road side fast food shops and small hotels, and 104 cooked samples from recognized fast food shops and restaurants were subjected to bacteriological isolation and identification. Highest number of Salmonella spp. were 42.5% in road side shops and 16.25% found in super market for dressed meat. Thigh muscle was more prevalent to breast muscle in both market types for dressed muscle. A total of 31 thigh muscles were positive for road side shop and 23 for super market where 26 and 19 breast muscle were positive for road side shop and super market respectively. Among the cooked meat or fried chicken incidence of Salmonella spp. were 23.86% in road side shops and 11.54% found in recognized shops. In case of both market source thigh muscle was highly prevalent in comparison to breast muscle. For cooked meat, a total of 19 thigh muscles were positive for road side fast food shops and small hotels, and 12 for recognized fast food shops and restaurants where 15 and 8 breast muscle were positive for road side fast food shops and recognized fast food shops respectively. Our results highlighted the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry products can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 40-44

scholarly journals Investigation of discriminate and indiscriminate use of doxycycline in broiler: an indoor research on antibiotic doxycycline residue study in edible poultry tissue

2020 ◽  
Vol 6 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Md Rostom Ali ◽  
Md Mahmudul Hasan Sikder ◽  
Md Shakil Islam ◽  
Md Shafiqul Islam
Keyword(s):  
Breast Muscle ◽  
Thigh Muscle ◽  
Control Group ◽  
Broiler Chicks ◽  
Withdrawal Period ◽  
Antibiotic Group ◽  
Group A ◽  
Group B ◽  
Tlc Analysis ◽  
Residue Study

Abuse of antibiotics is more common in developing countries including Bangladesh. The aim of this study was to detect doxycycline residue after discriminate and indiscriminate administration in broiler poultry. Eighteen broiler chicks, DOC (Cobb-500) was collected & reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely group–A (Control group), group-B (Discriminate group) and group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate groups were treated with antibiotic, doxycycline. In group-B withdrawal period was followed by treatment for 7 days. On the other hand, withdrawal period was not maintained in group-C, i.e. the antibiotic treatment was continued until the day of sacrifice. TLC analysis revealed that intensity of doxycycline in discriminate antibiotic group for liver, kidney, thigh muscle and breast muscle samples were found 63.33%, 65.33%, 22.67% and 26.00% respectively. And in indiscriminate antibiotic group intensity of doxycycline for liver, kidney, spleen, thigh muscle and breast muscle samples were found 50.67%, 50.33%, 39.50% and 48.17% respectively. All the samples of control group were found 0% intensity. The different among intensity were statistically significant. Overall, the present study documented the widespread abuse of doxycycline and failure to implement the recommended withdrawal period will undeniably leads to deposition of residues in broiler tissues. Asian J. Med. Biol. Res. March 2020, 6(1): 1-7

Concentrations of Antibiotic Residues Vary between Different Edible Muscle Tissues in Poultry

2005 ◽  
Vol 68 (10) ◽  
pp. 2217-2219 ◽  
Author(s):  
IXCHEL REYES-HERRERA ◽  
MARILYN J. SCHNEIDER ◽  
KIMBERLY COLE ◽  
MORGAN B. FARNELL ◽  
PAMELA J. BLORE ◽  
...  
Keyword(s):  
Federal Government ◽  
Thigh Muscle ◽  
Regulatory Agencies ◽  
Target Tissue ◽  
Antibiotic Residues ◽  
Withdrawal Period ◽  
Animal Tissues ◽  
Muscle Tissues ◽  
Specific Muscle ◽  
Antibiotic Residue

Antibiotics are used by veterinarians and producers to treat disease and improve animal production. The federal government, to ensure the safety of the food supply, establishes antibiotic residue tolerances in edible animal tissues and determines the target tissues (e.g., muscle) for residue monitoring. However, when muscle is selected as the target tissue, the federal government does not specify which type of muscle tissue is used for monitoring (e.g., breast versus thigh). If specific muscle tissues incorporate residues at higher concentrations, these tissues should be selected for residue monitoring. To evaluate this possibility in poultry, chickens were divided into four groups and at 33 days of age were dosed with enrofloxacin (Baytril), as per label directions, at either 25 ppm for 3 days, 25 ppm for 7 days, 50 ppm for 3 days, or 50 ppm for 7 days. Breast and thigh muscle tissues were collected from each bird (n = 5 birds per day per group) during the dosing and withdrawal period, and fluoroquinolone concentrations were determined. The results indicate higher overall enrofloxacin concentrations in breast versus thigh muscle for each treatment group (P &lt; 0.05). These data indicate, at least for enrofloxacin, that not all muscle tissues incorporate antibiotics at the same concentrations. These results may be helpful to regulatory agencies as they determine what tissues are to be monitored to ensure that the established residue safety tolerance levels are not exceeded.

scholarly journals A Wake-Up Call: Determination of Antibiotics Residue Level in Raw Meat in Abattoir and Selected Slaughterhouses in Five Local Government in Kano State, Nigeria.

2022 ◽  
Author(s):  
Lateefat Modupe Habeeb ◽  
Opasola Afolabi Olaniyi ◽  
Misbahu Garba ◽  
Morufu O Raimi
Keyword(s):  
Bacterial Resistance ◽  
Antibiotic Sensitivity ◽  
Human Consumption ◽  
Withdrawal Period ◽  
Financial Loss ◽  
Cross Sectional ◽  
Raw Meat ◽  
Maximum Residue Limits ◽  
Antibiotics Residues ◽  
Tetracycline Residues

The frequent use of antibiotics may result in drug residues that can be discovered at varying quantities in animal products such as milk or meat. The presence of pharmaceutical residues in food above the MRLs has been perceived globally by various persons. Antibiotics residues are present in food, which can endanger human health by causing antibiotic sensitivity, allergic reactions, microflora imbalance, bacterial resistance to antibiotics in microorganisms, and financial loss to the food industry. Farmers around the world utilize them on a sporadic basis for both preventative and curative purposes. This study assessed the antibiotics residues in raw meat sold in 6 slaughter houses in Kano States. The study is a descriptive cross-sectional study involving six (6) major slaughter house in Kano state. Muscle, Kidney and liver samples were collected from each slaughterhouse. The antibiotic residues in the meat samples were analysed using high performance liquid chromatography (HPLC) for tetracycline, ciprofloxacin and oxytetracycline residue results were presented in charts and tables. Out of a total of 18 beef samples analyzed during this study, 15 (83%) of the total samples had detectable levels of tetracycline residues from which 6(33.3%) had tetracycline residues at violative levels above the WHO/FAO maximum residue limits (MRLs), out of those 18 beef samples analyzed during this study, 6(33%) of the total samples had detectable levels of oxytetracycline residues from which 3(17%) had oxytetracycline residues at violative levels above the WHO/FAO maximum residue limits (MRLs) and out of those 18 beef samples analyzed during this study, 12(67%) of the total samples had detectable levels of ciprofloxacin, all levels are below the WHO/FAO maximum residue limits (MRLs). This high level of tetracycline and oxytetracycline residues in greater proportion of meat destined for human consumption at violative levels could be as a result of the indiscriminate use and misuse of veterinary drugs as commonly practiced among livestock producers and marketers without observing withdrawal period prior to slaughter. These results indicate that consumers may be predisposed to health hazards and hinder international meat trade from Nigeria. Regulatory authorities should therefore ensure compliance with good agricultural practices including withdrawal period of drugs used for treatment of food animals.

scholarly journals Heavy metal residues in offals, muscle and eggs of intensively reared poultry birds in Umuahia, Abia State

2021 ◽  
pp. 15-19
Keyword(s):  
Public Health ◽  
Poultry Meat ◽  
Health Concern ◽  
Thigh Muscle ◽  
Human Consumption ◽  
Poultry Products ◽  
Poultry Birds ◽  
Permissible Levels ◽  
The Mean ◽  
Kidney Liver

Poultry meat and poultry products have gradually become delicacy in Nigeria and are used in most celebrations for entertainment. Therefore there is the need for constant monitoring to ensure they are wholesome and suitable for human consumption. The aim of this study was to determine the presence and concentrations of Manganese (Mn), Zinc (Zn) and Iron (Fe) in the thigh muscle and offals (kidney, liver and gizzard) of both broilers and layers reared under intensive system of management using an Atomic Absorption Spectrophotometer (AAS). In both broilers and layers, all meat and offal samples had concentrations of Mn and Fe that were above the maximum permissible levels (MPL) stipulated by FAO/WHO. In layers, the concentrations of Mn (5.276 ± 0.023mg/kg) and Fe (7.067 ± 0.569mg/kg) were highest in the liver. The concentration of Zn (5.039 ± 0.009mg/kg) was found to be highest in the kidney. In broilers, Mn (5.105 ± 0.053mg/kg) accumulated more in the thigh muscle than any other organ whereas the highest concentration of Fe (6.256 ± 0.246 mg/kg) was found in the liver. The mean concentration of Mn, Zn and Fe in eggs was 5.081 ± 0.033, 5.092 ± 0.021 and 7.102 ± 0.146 (mg/kg) respectively. The concentration on Mn in eggs was slightly above the maximum residual limits of 5.0 mg/kg. The high mean concentrations of Mn and Fe found in the study are of great public health concern because these metals are bioaccumulation and with continuous consumption, may pose a serious threat to public health.

scholarly journals Mineral Element Deposition and Gene Expression across Different Tissues of Cherry Valley Ducks

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 238
Author(s):  
Qianqian Song ◽  
Yi Zhang ◽  
Hao Bai ◽  
Li Zhong ◽  
Xiaofan Li ◽  
...  
Keyword(s):  
Significant Negative Correlation ◽  
Mineral Elements ◽  
Breast Muscle ◽  
Thigh Muscle ◽  
Mrna Levels ◽  
High Quality ◽  
Atomic Fluorescence ◽  
Breeding Efficiency ◽  
Almost All ◽  
Different Tissues

This study was conducted to investigate the deposition of several mineral elements and the mRNA levels of mineral-related genes across different tissues of cherry valley ducks. The contents of magnesium (Mg), potassium (K), zinc (Zn), and selenium (Se) in ducks’ breast muscle, thigh muscle, liver, skin, and tibia at the age of 0, 21, 35, 49, and 63 days, respectively, were measured using an atomic fluorescence spectrophotometer, while the mRNA levels of mineral-related genes were detected by qRT-PCR. The results revealed that the dynamics of Mg and K were generally similar in each tissue, with a significant positive correlation (p < 0.05). In the breast muscle, thigh muscle, and liver, the contents of almost all mineral elements reached their peak values (p < 0.05) at the age of 49 to 63 days. Interestingly, the expression of most mineral-related genes was the highest at birth (p < 0.05). In addition, there was a significant negative correlation between the expression of ATP1A1 and the deposition of K (r = −0.957, p < 0.05), and a similar result was found for the expression of ATP8 and the deposition of Zn (r = −0.905, p < 0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of the high-quality strain of cherry valley ducks, while the age of 49 to 63 days could be used as the reference for the best marketing age. In addition, ATP1A1 and ATP8 could be used as the key genes to detect K and Zn, respectively. Hence, the findings of this study can be used to improve the production and breeding efficiency of high-quality meat ducks.

scholarly journals Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1456
Author(s):  
Sandra Sevilla-Navarro ◽  
Pablo Catalá-Gregori ◽  
Clara Marin
Keyword(s):  
Point Of View ◽  
Poultry Production ◽  
Poultry Products ◽  
Production Type ◽  
Poultry Farms ◽  
Commercial Poultry ◽  
Salmonella Serovars ◽  
The Impact ◽  
Phage Isolation ◽  
Salmonella Phage

The exploration of novel nonantibiotic interventions in the field, such as the use of bacteriophages, is necessary to avoid the presence of Salmonella. Bacteriophages are a group of viruses widely distributed in nature, strictly associated with the prokaryotic cell. Researchers have demonstrated the success of phage therapy in reducing Salmonella counts in poultry products. However, the impact that phage concentration in the environment may have against certain Salmonella serovars is not well understood. Therefore, the aim of this study was to assess Salmonella phage prevalence in commercial poultry farms in terms of the production type: layers or broilers. The most prevalent Salmonella serovars isolated in poultry production were used for phage isolation. Salmonella specific phages were isolated from 141 layer and broiler farms located in the Valencia region during 2019. Analysis of the samples revealed that 100% presented Salmonella phages, the most prevalent being against serovar S. Enteritidis (93%), followed by S. Virchow (59%), S. Typhimurium (55%), S. Infantis (52%) and S. Ohio (51%). These results indicate that poultry farms could represent an important source of Salmonella phages. Nevertheless, further studies are needed to assess the epidemiology of phages against other serovars present in other countries and their diversity from the point of view of molecular studies.

Consumption pattern of chicken and consumer awareness of unwholesome practises by some dressed chicken smugglers In Oyo Town, Nigeria

2021 ◽  
Vol 17 (1) ◽  
pp. 71-76
Author(s):  
O.O. Okunlola
Keyword(s):  
Food Safety ◽  
Sampling Method ◽  
Tertiary Education ◽  
Consumption Pattern ◽  
Consumer Awareness ◽  
Second Stage ◽  
The People ◽  
Poultry Farms ◽  
Multistage Sampling ◽  
The Town

Oyo town has a large concentration of poultry farms in Oyo state. This will in no small way influence the meat choice of the people of the town. This study was carried out to determine the consumption pattern of chicken and the unwholesome practices by dressed chicken smugglers in Oyo town, Nigeria. A total of 100 questionnaires were purposively administered to chicken consumers in thestudy area. Also, a multistage sampling method was adopted with the study area divided into three, on Local Government Area (LGA) basis. in the first stage. In the second stage, 30 questionnaires were administered in each of Oyo East and Oyo West LGAs while 40 were administered in Atiba LGA. Data from the table show that majority of the respondents were female (62%), aged between 20 – 39 years (46%) and had tertiary education (54%). From the table, 22% claimed affordability as the reason for chicken purchase while 72% preferred dressed chicken to live chicken.The results also show that 56% of the respondents ate chicken weekly and 47% of them made their purchases from farms and farm outlets. Factors considered to influence purchases were affordability (30%) and availability (24%). Only twenty four percent (24%) of the respondents were aware of the unwholesome practices by dressed chicken smugglers while 46% of those aware saw the consumption of smuggled chicken as hazardous. Key words: Oyo town, chicken consumption, chicken preferences, dressed chicken smugglers, food safety

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