Commutability assessment of reference materials for the enumeration of lymphocyte subsets

2019 ◽  
Vol 57 (5) ◽  
pp. 697-706 ◽  
Author(s):  
Chenbin Li ◽  
Mingting Peng ◽  
Dongsheng Xu ◽  
Hong Lu ◽  
Wenbin Zhou ◽  
...  

Abstract Background Flow cytometric enumeration of lymphocyte subsets in peripheral blood can provide important information about immune status. Commutable reference materials (RM) are crucial for maintaining accurate and comparable measurement results over time and space. Commutability assessment of RMs for lymphocyte subsets enumeration has not been reported elsewhere. Methods Lymphocyte subsets were measured in triplicate on 56 patient samples and eight RMs using two measuring systems commonly used in laboratories (FACS Canto II and Cytomics FC500). The first step was to determine the suitability of RMs and comparability of different systems with patient samples. After the requirements of suitability and comparability were met, the second step was to assess commutability following regression approach and difference in bias approach. Results Two RMs were not measurable on FC500 system for CD3-CD16/56+ and CD3-CD19+ percentages. The results of comparability showed no significant difference in the two systems. Eight RMs for CD3+CD4+ cell count, six RMs for CD3+ and CD3+CD8+ percentages, five RMs for CD3-CD16/56+ percentage, and three RMs for CD3-CD19+ percentage were commutable using the two approaches. For CD3+, CD3+CD8+ and CD3-CD19+ percentages, the results of regression approach showed that one RM was non-commutable for each parameter, while the other approach showed that the RM was commutable. Conclusions The suitability of RM and comparability of different measuring systems are prerequisites for assessing commutability. This study indicated that different approaches led to different results. The difference in bias approach is recommended for criteria relating to medical requirements and performance characteristics of measuring systems in use.

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Olusola Ojurongbe ◽  
Oluwatoyin Adeola Oyeniran ◽  
Oyebode Armstrong Terry Alli ◽  
Sunday Samuel Taiwo ◽  
Taiwo Adetola Ojurongbe ◽  
...  

Malaria and HIV are the two most important health challenges of our time. Haematologic abnormalities are features inPlasmodium falciparuminfection, and anaemia is a well-known outcome. The prevalence and haematological impact ofP. falciparumparasitaemia were determined among HIV-infected individuals in Nigeria. Parasite detection was carried out using microscopy and Polymerase Chain Reaction (PCR). Haemoglobin concentration was determined using an automated machine while CD4+ T-cells count was analyzed using flow cytometer. Thirty-seven (18.5%) out of the 200 HIV individuals enrolled had malaria parasites detected in their blood. All the positive cases were detected by PCR while only 20 (10%) were detected by thick blood microscopy. The mean haemoglobin concentration and packed cell volume (PCV) of HIV individuals with malaria parasitaemia were lower compared to those without malaria parasitaemia but the difference was not statistically significant. Also no significant difference was observed in malaria positivity in respect to sex and mean CD4+ cell count. The study highlights the effects ofP. falciparumparasitaemia on the haematologic and immune components of HIV individuals.


Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3764-3764
Author(s):  
Zaher Otrock ◽  
Sami Azar ◽  
Adlette Inati ◽  
Suzane Koussa ◽  
Rami Mahfouz ◽  
...  

Abstract Background: Beta-thalassemia is an inherited disorder of hemoglobin synthesis with many associated morbidities. Osteoporosis is an important cause of morbidity in these patients. Bisphosphonates are potent inhibitors of osteoclast activity and have been recently used for the treatment of osteoporosis in beta-thalassemia. Several studies have indicated that the vitamin D receptor (VDR) gene has a role in the regulation of bone mineral density (BMD) and bone metabolism, but the results are very controversial. The aim of this study is to assess the effectiveness and safety of zoledronic acid in Lebanese thalassemics with osteoporosis. Methods: We studied 23 patients with beta-thalassaemia major (14 patients) and intermedia (9 patients). Thirteen patients were male and 10 were female, with a mean (+/− standard deviation) age of 25 +/− 9 years. All patients had severe osteoporosis and were receiving calcium and vitamin D supplements prior to and during the study. Zoledronic acid was given i.v. at a dose of 4 mg every 3 months over 12 months. The effects were monitored by assessing pain score, analgesic score and performance score. The BMD of the hip and lumbar spine, osteocalcin, bony alkaline phosphatase and pyrrolidine crosslinks were also measured. To gain more insight into the genetic basis of osteoporosis in thalassemia and to correlate this with the response to zoledronic acid, we analysed the polymorphism of the VDR gene in three polymorphic sites (Bsm I, Taq I and Apa I) using restriction enzymes digestion. Results: We have given two doses of zoledronic acid uptil now. The most common adverse event was joint pain in 12 patients (52.2%) lasting for 1–3 days and responding to analgesics. Four patients had perioral numbness and five had fever. These adverse events were markedly reduced after the 2nd dose; only two patients had joint pain. No patients discontinued the study until now. The pain scores, analgesic score and performance score were all improved compared to baseline values. The difference in pain index score was significant (p=0.004), and the reduction in the number of painful sites was slightly significant (p=0.097). There was significant difference in the baseline z score of the spine between transfusion dependent patients (15 patients) and transfusion non-dependent patients (8 patients) (p = 0.042). The distributions of VDR alleles in Bsm I polymorphism were 30.4% for BB, 52.3% for Bb, and 17.3% for bb; in the Apa I polymorphism 17.4% for AA, 47.8% for Aa, and 34.8% for aa; in Taq I polymorphism 30.4% for TT, 52.3% for Tt, and 17.3% for tt. We found no significant differences in baseline BMD between the three groups of Bsm I polymorphism at all sites. Apa I was not associated with significant differences in BMD. The BMD of the Ward’s triangle was higher in patients with the Tt-genotype compared with individuals with the tt-genotype and the difference was almost significant (p=0.089, ANOVA). Conclusions: In Lebanese thalassemics with osteoporosis zoledronic acid (a dose of 4 mg) is well tolerated and the clinical response is quite impressive. However, the response to treatment needs to be assessed by follow-up BMDs and blood bone markers.


2021 ◽  
Vol 10 (8) ◽  
pp. 1728
Author(s):  
Simon Rauch ◽  
Michiel Jan van Veelen ◽  
Rosmarie Oberhammer ◽  
Tomas Dal Cappello ◽  
Giulia Roveri ◽  
...  

Cardiopulmonary resuscitation (CPR) is considered an aerosol-generating procedure. Consequently, COVID-19 resuscitation guidelines recommend the use of personal protective equipment (PPE) during resuscitation. In this simulation of randomised crossover trials, we investigated the influence of PPE on the quality of chest compressions (CCs). Thirty-four emergency medical service BLS-providers performed two 20 min CPR sequences (five 2 min cycles alternated by 2 min of rest) on manikins, once with and once without PPE, in a randomised order. The PPE was composed of a filtering facepiece 3 FFP3 mask, safety glasses, gloves and a long-sleeved gown. The primary outcome was defined as the difference between compression depth with and without PPE; secondary outcomes were defined as differences in CC rate, release and the number of effective CCs. The participants graded fatigue and performance, while generalised estimating equations (GEE) were used to analyse data. There was no significant difference in CC quality between sequences without and with PPE regarding depth (mean depth 54 ± 5 vs. 54 ± 6 mm respectively), rate (mean rate 119 ± 9 and 118 ± 6 compressions per minute), release (mean release 2 ± 2 vs. 2 ± 2 mm) and the number of effective CCs (43 ± 18 vs. 45 ± 17). The participants appraised higher fatigue when equipped with PPE in comparison to when equipped without PPE (p < 0.001), and lower performance was appraised when equipped with PPE in comparison to when equipped without PPE (p = 0.031). There is no negative effect of wearing PPE on the quality of CCs during CPR in comparison to not wearing PPE.


2017 ◽  
Vol 14 (2) ◽  
pp. 1492
Author(s):  
Murat Akyüz ◽  
Murat Özmaden ◽  
Yeliz Doğru ◽  
Evren Karademir ◽  
Yeşim Aydın ◽  
...  

This study was conducted to examine the effects of static and dynamic stretching exercises on strength, flexibility, speed and balance in young basketball players. 10 volunteers from the 16 year old male basketball team of Pınar Karsiyaka Sports Club participated in the study. The average age of the athletes is 16 ± 0,0 (year) and the average of the height is 188 ± 0,08 (cm). The study consists of three parts. On the first day, dynamic stretching exercises were performed on the second day and static stretching exercises and performance tests were performed on the third day without performing any exercise. Applied performance tests were hand grip strength, back-leg strength, sit-in flexibility test, 20 m. Sprint test and flamingo balance test. The obtained data were analyzed with the variance analysis (ANOVA test) in the repeated measures using the SPSS 23.0 package program. As a result of the study, the flexibility of the static and dynamic stretching exercises in the data obtained, the difference in the left handed claw strength and the speed of the dynamic stretching exercises were significant (p <0.05). On the other hand, no statistically significant difference was found between static and dynamic stretching exercises and back leg strength and balance (p> 0.05). As a result, it can be said that static and dynamic stretching exercises have a positive effect on flexibility, strength and speed. ÖzetBu çalışma genç basketbolcularda statik ve dinamik germe egzersizlerinin kuvvet, esneklik, sürat ve denge üzerindeki etkisini incelemek amacıyla yapılmıştır. Çalışmaya Pınar Karşıyaka Spor Kulübünün 16 yaş erkek basketbol takımından 10 gönüllü katılmıştır. Sporcuların yaş ortalamaları 16±0,0 (yıl), boy ortalamaları 188±0,08 (cm)’dir. Çalışma üç bölümden oluşmaktadır. Birinci gün herhangi bir egzersiz uygulanmadan, ikinci gün dinamik germe egzersizleri ve üçüncü gün statik germe egzersizleri ile performans testleri uygulanmıştır. Uygulanan performans testleri sırasıyla el kavrama kuvveti, sırt-bacak kuvveti, otur-eriş esneklik testi, 20 m. sprint testi ve flamingo denge testinden oluşmaktadır. Elde edilen veriler SPSS 23.0 paket programı kullanılarak tekrarlı ölçümlerde Varyans Analizi (Anova Testi) ile analiz edilmiştir. Çalışma sonucunda elde edilen verilerde statik ve dinamik germe egzersizlerinin esnekliğe, dinamik germe egzersizlerinin sol el pençe kuvveti ve sürat üzerinde anlamlı bir farklılık meydana gelmiştir (p<0.05). Diğer yandan, statik ve dinamik germe egzersizleri ile sırt bacak kuvveti ve denge üzerinde anlamlı bir farklılık bulunamamıştır (p>0.05). Sonuç olarak, statik ve dinamik germe egzersizlerinin esneklik, kuvvet ve sürat üzerinde olumlu etkisi olduğu söylenebilir.


2020 ◽  
Vol 36 (7) ◽  
Author(s):  
Yang Li ◽  
Han-liang Jiao ◽  
Yu-kun Bai ◽  
Ping Wang

Objective: To explore the effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception. Methods: This is a prospective randomized controlled study in which 60 children with primary intussusception admitted to Hebei Children’s Hospital from October 2018 to October 2019 were selected for this study. They were randomly divided into two groups. The 30 patients in the experimental group underwent manipulative reduction and air enema reduction, and 30 patients in the control group underwent only air enema reduction. Pain scores and pressure during enema were recorded and analyzed. Fasting blood of children in the experimental group were drawn to test the serum T lymphocyte subsets CD3+, CD4+, CD8+ levels, B lymphocyte subsets CD19+ level, and NK cell subsets CD56+ levels before reduction. Among them, fasting blood of 28 children with successful reduction were drawn again in the morning after reduction, and the indicators of each immune cell subgroup before and after reduction were analyzed. Two children with unsuccessful reduction were no longer tested for these indicators. Results: Twenty-Eight children in the experimental group had successful reduction, and two children with unsuccessful reduction were changed to open surgery (28/30). Twenty five Children in the control group had successful reduction, and five were changed to open surgery (25/30). There was no significant difference in the success rate of reduction between two groups (p>0.05). Close observation for 12~24h after reduction found that none of the children had signs of peritonitis. The pain score and reduction pressure of the observation group were lower than those of the control group, and the difference was statistically significant (p<0.05). The levels of serum CD3+, CD4+, and CD8+ after reduction in the experimental group were significantly higher than before reduction, and the difference was statistically significant (p<0.05). CD19+ level was significantly lower than before reduction, and the difference was statistically significant (p<0.05). There was no significant difference in changes of other indicators. Conclusions: Manipulative reduction combined with air enema reduction can relieve pain and air injection pressure during enema, reduce reperfusion injury caused by intestinal ischemia, and protect intestinal mucosal immune function, which is a favored treatment. doi: https://doi.org/10.12669/pjms.36.7.3105 How to cite this:Li Y, Jiao H, Bai Y, Wang P. Effect of manipulative reduction combined with air enema on intestinal mucosal immune function in children with intussusception. Pak J Med Sci. 2020;36(7):---------. doi: https://doi.org/10.12669/pjms.36.7.3105 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


2018 ◽  
Vol 8 (1) ◽  
pp. 39
Author(s):  
Nicky Prima Yudha ◽  
Syahrul Ramadhan ◽  
Ngusman Abdul Manaf

This study was conducted to explain (a) the difference in narrative essay writing skills of students who are taught using cooperative learning model CIRC with Jigsaw (b) differences in the ability to write narrative essays highly motivated students who are learning with cooperative learning model CIRC with Jigsaw; (c) differences in the ability to write narrative essays low motivated students who are learning to cooperative learning model CIRC with Jigsaw; and (d) the interaction between the use of models in the study of learning and motivation affect the ability of students to write a narrative essay. This study was a quasi-experimental (quasi-experiment) using a 2x2 factorial design. Population of 250 people, the study sample is taken by means of random sampling and obtained class VII / 2 as an experimental class I and class VII / 1 as the experimental class II, each consisting of 30 and 32 students. Data were collected by two instruments, namely motivation questionnaire and performance test writing narrative essays. Analyzing and discussion of the data is done by descriptive-analytical. Based on the results of data analysis, we can conclude the following four things. First, there are no significant differences in narrative essay writing skills of students who are taught using cooperative learning model CIRC with Jigsaw. Secondly, there is no significant difference in the ability to write narrative kararangan highly motivated students who are learning with cooperative learning model CIRC and Jigsaw. Third, there is no significant difference in the ability to write narrative kararangan low motivated students who are learning to cooperative learning model CIRC and Jigsaw. Fourth, there is no interaction between the model of learning with learning motivation in influencing the ability to write narrative essays seventh grade students of SMP Negeri 28 Padang. The use of models and Jigsaw cooperative CIRC can enhance students' ability to write a narrative essay, motivated either high or low.


2016 ◽  
Vol 10 (09) ◽  
pp. 982-987 ◽  
Author(s):  
Juan Carlos Domínguez-Hermosillo ◽  
José Antonio Mata-Marin ◽  
Norma Estela Herrera-González ◽  
Marcelino Chávez-García ◽  
Gloria Huerta-García ◽  
...  

Introduction: Although both tipranavir (TPV) and darunavir (DRV) represent important options for the management of patients with multi-protease inhibitor (PI)-resistant human immunodeficiency virus (HIV), currently there are no studies comparing the effectiveness and safety of these two drugs in the Mexican population. The aim of this study was to compare the effectiveness of TPV versus DRV as a salvage therapy in HIV-1 treatment-experienced patients. Methodology: This was a comparative, prospective, cohort study. Patients with HIV and triple-class drug resistance evaluated at the Hospital de Infectología “La Raza”, National Medical Center, were included. All patients had the protease and retrotranscriptase genotype; resistance mutation interpretation was done using the Stanford database. Results: A total of 35 HIV-1 triple-class drug-resistant patients were analyzed. All of them received tenofovir and raltegravir, 22 received darunavir/ritonavir (DRV/r), and 13 received tipranavir/ritonavir (TPV/r) therapies. The median baseline RNA HIV-1 viral load and CD4+ cell count were 4.34 log (interquartile range [IQR], 4.15–4.72) and 267 cells/mm3 (IQR, 177–320) for the DRV/r group, and 4.14 log (IQR, 3.51–4.85) and 445 cells/mm3 (IQR, 252–558) for the TPV/r group. At week 24 of treatment, 91% of patients receiving DRV/r and 100% of patients receiving TPV/r had an RNA HIV-1 viral load < 50 copies/mL and a CD4+ cell count of 339 cells/mm3 (IQR, 252–447) and 556 cells/mm3 (IQR, 364–659), respectively. Conclusions: No significant difference was observed between DRV/r and TPV/r in terms of virological suppression in HIV-1 patients who were highly experienced in antiretroviral therapy.


Author(s):  
Monta Madelāne ◽  
Angelika Krūmiņa ◽  
Raimonds Sīmanis ◽  
Ģirts Šķenders ◽  
Andrejs Ivanovs ◽  
...  

Abstract Immune activation in human immunodeficiency virus (HIV) infection is driven by microbial translocation and in HIV patients is one of the contributors to faster progression of liver disease along with increased cell apoptosis. The aim of the study was to compare microbial translocation and apoptosis markers in HIV monoinfected and HIV/hepatitis C virus (HCV) coinfected patients, depending on HIV immune status and antiretroviral treatment (ART). We analysed data for 78 HIV monoinfected and 105 HIV/HCV coinfected patients from the Rīga East University Hospital. Lipopolysaccharide (LPS), endotoxin core antibodies (EndoCAb), cytokeratin 18 (CK18) and cyto-chrome c (Cyt-c) levels were measured. No significant difference in LPS, EndoCAb, Cyt-c levels between HIV and HIV/HCV patients was found. The CK18 level was higher in the HIV/HCV group. Correlation between CD4+ cell count and EndoCAb antibodies was found in HCV positive patients. There was a significant effect of ART on markers for EndoCAb IgA and EndoCAb IgM antibodies in the HIV monoinfected group. Correlation between CD4+ cell count and EndoCAb antibodies and LPS was found in HIV/HCV patients on ART. Coinfection with HCV can lead to more pronounced response in EndoCAb antibody production and higher levels of cell apoptosis markers, despite similar LPS levels. ART has a positive effect on immune activation.


2020 ◽  
Vol 12 (23) ◽  
pp. 10215
Author(s):  
Vlastimil Chytrý ◽  
Janka Medová ◽  
Jaroslav Říčan ◽  
Jiří Škoda

In research focused on self-efficacy it is usually teacher-related phenomena that are studied, while the main aspects related to pupils are rather neglected, although self-efficacy itself is perceived as a belief in one’s own abilities. Evidently, this strongly influences the behavior of individuals in terms of the goal and success in mathematical problem-solving. Considering that alternative teaching methods are based on the principle of belief in one’s own ability (mainly in the case of group work), higher self-efficacy can be expected in the pupils of teachers who use predominantly the well-working pupil-centered pedagogies. A total of 1133 pupils in grade 5 from 36 schools in the Czech Republic were involved in the testing of their ability to solve mathematical problems and their mathematical self-efficacy as well. Participants were divided according to the above criteria as follows: (i) 73 from Montessori primary schools, (ii) 332 pupils educated in mathematics according to the Hejný method, (iii) 510 pupils from an ordinary primary school, and (iv) 218 pupils completing the Dalton teaching plan. In the field of mathematical problem-solving the pupils from the Montessori primary schools clearly outperformed pupils from the Dalton Plan schools (p = 0.027) as well as pupils attending ordinary primary schools (p = 0.009), whereas the difference between the Montessori schools and Hejný classes was not significant (p = 0.764). There is no statistically significant difference in the level of self-efficacy of pupils with respect to the preferred strategies for managing learning activities (p = 0.781). On the other hand, correlation between mathematical problem-solving and self-efficacy was confirmed in all the examined types of schools. However, the correlation coefficient was lower in the case of the pupils from the classes applying the Hejný method in comparison with the pupils attending the Montessori schools (p = 0.073), Dalton Plan schools (p = 0.043), and ordinary primary schools (p = 0.002). Even though the results in mathematical problem-solving are not consistent across the studies, the presented results confirm better performance of pupils in some constructivist settings, particularly in the case of individual constructivism in the Montessori primary schools. The factors influencing lower correlation of self-efficacy and performance in mathematical problem-solving ought to be subject to further investigation.


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