International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine Position on bone marker standards in osteoporosis

2011 ◽  
Vol 49 (8) ◽  
Author(s):  
Samuel Vasikaran ◽  
Cyrus Cooper ◽  
Richard Eastell ◽  
Andrea Griesmacher ◽  
Howard A. Morris ◽  
...  
Keyword(s):  
Fracture Risk ◽  
Type I Collagen ◽  
Clinical Chemistry ◽  
Laboratory Medicine ◽  
International Federation ◽  
Bone Marker ◽  
Bone Resorption Marker ◽  
Type I ◽  
International Osteoporosis Foundation ◽  
Treatment Research

AbstractThe International Osteoporosis Foundation (IOF) and the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Working Group on Bone Marker Standards (WG-BMS) has evaluated the clinical potential of bone turnover markers (BTMs) in the prediction of fracture risk and for monitoring treatment. Research evidence suggests that BTMs may provide information on fracture risk independently from BMD, so that fracture risk prediction might be enhanced by their inclusion in assessment algorithms. The potential use of BTMs to predict the response to treatments for osteoporosis in the individual patient is also of great interest. Treatment-induced changes in specific markers account for a substantial proportion of fracture risk reduction. However, there is still a need for stronger evidence on which to base practice in both situations. IOF/IFCC recommends one bone formation marker (serum procollagen type I N propeptide, s-PINP) and one bone resorption marker (serum C-terminal cross-linking telopeptide of type I collagen, s-CTX) to be used as reference markers and measured by standardised assays in observational and intervention studies in order to enlarge the international experience of the application of markers to clinical medicine and to help resolve uncertainties over their clinical use.

scholarly journals High sensitivity, contemporary and point-of-care cardiac troponin assays: educational aids developed by the IFCC Committee on Clinical Application of Cardiac Bio-Markers

2019 ◽  
Vol 57 (5) ◽  
pp. 623-632 ◽  
Author(s):  
Paul O. Collinson ◽  
Amy K. Saenger ◽  
Fred S. Apple ◽  
Keyword(s):  
Cardiac Troponin ◽  
Task Force ◽  
Clinical Chemistry ◽  
Point Of Care ◽  
High Sensitivity ◽  
Laboratory Medicine ◽  
International Federation ◽  
Cardiac Biomarkers ◽  
Educational Materials ◽  
Extensive Information

AbstractThe International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) formed a Task Force on the Application of Cardiac Bio-markers (TF-CB) in 2008, re-designated in 2018 as a committee (C-CB), to produce educational materials on cardiac biomarkers. Established in June 2017, definitive tables covering the majority of high-sensitivity, contemporary and point-of-care (POC) cTn assays have been developed by the C-CB and are available on the IFCC website. These tables provide extensive information about assays’ analytical characteristics and encompass information on diagnostic discriminants, particularly the 99th percentiles, as provided by the manufacturers.

scholarly journals Responses of Markers of Bone and Collagen Turnover to Exercise, Growth Hormone (GH) Administration, and GH Withdrawal in Trained Adult Males1

2000 ◽  
Vol 85 (1) ◽  
pp. 124-133 ◽  
Author(s):  
Jennifer D. Wallace ◽  
Ross C. Cuneo ◽  
Per Arne Lundberg ◽  
Thord Rosén ◽  
Jens Otto Lunde Jørgensen ◽  
...  
Keyword(s):  
Acute Exercise ◽  
Type I Collagen ◽  
Controlled Study ◽  
Bone Resorption Marker ◽  
Type I ◽  
Serum Osteocalcin ◽  
Collagen Turnover ◽  
Gh Treatment ◽  
Type I Procollagen ◽  
Procollagen Iii

To examine the interactions between acute exercise and GH on markers of bone and collagen turnover and to assess the potential for detecting GH abuse in athletes using these markers, we studied 17 aerobically trained males (age, 26.9 ± 1.5 yr). Sequential studies of exercise, GH administration, and GH withdrawal were undertaken. A randomized, controlled study of rest vs. exercise showed that exercise did not change serum osteocalcin; other markers of formation increased transiently (each P < 0.001): bone-specific alkaline phosphatase (+16.1%), carboxyterminal propeptide of type I procollagen (+14.1%), and procollagen III N-terminal extension peptide (+5.0%). The carboxyterminal cross-linked telopeptide of type I collagen, a bone resorption marker, increased 9.7% (P = 0.018) in response to exercise. A randomized, double blind, placebo-controlled, parallel study of recombinant human GH treatment (0.15 IU/kg·day) for 1 week increased serum osteocalcin (net increase preexercise, +10.0%; P = 0.017), carboxyterminal propeptide of type I procollagen (+17.6%; P = 0.002), procollagen III N-terminal extension peptide (+48.4%; P = 0.001), and carboxyterminal cross-linked telopeptide of type I collagen (53.3%; P = 0.009). Disappearance half-times after cessation of recombinant human GH for pre- and postexercise markers ranged from 248–770 h. We conclude 1) endurance exercise transiently activates bone and collagen turnover; 2) brief GH administration results in similar but quantitatively greater augmentation; and 3) these data will assist in designing a GH detection strategy.

Diurnal variation in concentrations of various markers of bone metabolism in growing female goats and sheep

2003 ◽  
Vol 77 (2) ◽  
pp. 197-203 ◽  
Author(s):  
A. Liesegang ◽  
M.-L. Sassi ◽  
J. Risteli
Keyword(s):  
Type I Collagen ◽  
Bone Markers ◽  
Degradation Products ◽  
Bone Marker ◽  
Type I ◽  
Blood Samples ◽  
Bone Specific Alkaline Phosphatase ◽  
Growing Goats ◽  
Turn Over ◽  
Carboxyterminal Telopeptide

AbstractTwelve 6-month-old growing female goats and sheep were used in this study. Blood samples were obtained in the morning before goats and sheep were given food and then at 2-h intervals for 24 h (part I). This procedure was repeated 2 weeks later (part II). Concentrations of osteocalcin (OC), activities of total (tAP) and bone-specific alkaline phosphatase (bAP), degradation products of C-terminal telopeptide of type-I collagen (CrossLaps™ CL), and carboxyterminal telopeptide of type-I collagen (ICTP) were measured in serum.In both parts of the study, all bone marker concentrations were significantly higher in goats than in sheep. The OC concentrations in goats increased in the late afternoon/evening and decreased thereafter to reach values similar to those obtained at the beginning. The ICTP concentrations in goats slowly decreased until 14:00 h, increased, and decreased again. The concentrations in sheep decreased continuously but not significantly, towards the morning sampling. The CL concentrations increased in both sheep and goats during the night but at 06:00 h started to decrease to levels found at the beginning of testing. The bAP activities decreased in goats from 20:00 to 22:00 h. Changes in the concentrations of bone markers were mainly observed in goats of this study. As documented for bone resorption and formation in other species, circadian rhythms were evident for concentrations of ICTP, CL, bAP and OC. The present study indicates that growing goats may have a physiologically higher bone turn-over than growing sheep, because the bone marker concentrations were always higher.

Immunoassay for quantifying type I collagen degradation products in urine evaluated

1994 ◽  
Vol 40 (11) ◽  
pp. 2022-2025 ◽  
Author(s):  
M Bonde ◽  
P Qvist ◽  
C Fledelius ◽  
B J Riis ◽  
C Christiansen
Keyword(s):  
Type I Collagen ◽  
Degradation Products ◽  
Mean Value ◽  
Collagen Degradation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bone Resorption Marker ◽  
Type I ◽  
Analytical Recovery ◽  
Collagen Degradation Products ◽  
Better Than

Abstract An enzyme-linked immunosorbent assay (ELISA) for measuring type I collagen degradation products in urine < 3 h was evaluated. The measuring range was 0.5-10.5 mg/L with a detection limit of 0.2 mg/L. Within-run and total CVs were 5.3% and 6.6%, respectively. Analytical recovery averaged 100%. The mean (+/- SD) concentrations in urine samples from a healthy premenopausal population (n = 102) were 250 +/- 110 mg/mol creatinine (Cr). A group of healthy postmenopausal women (n = 410) gave a mean value of 416 +/- 189 mg/mol Cr. Values obtained in the ELISA correlated well (r = 0.83) to HPLC values for the established bone resorption marker deoxypyridinoline (n = 214), slightly better than the correlation to hydroxyproline measurements (r = 0.78, n = 421). We conclude that the assay described here presents a useful tool for further elucidating the importance of type I collagen degradation products in urine.

Referenzbereiche von Insulin, insulinähnlichem Wachstumsfaktor 1 (IGF-1) und adrenokortikotropem Hormon bei Ponys

2016 ◽  
Vol 44 (01) ◽  
pp. 19-25 ◽  
Author(s):  
G. Köller ◽  
K. Bassewitz ◽  
G. F. Schusser
Keyword(s):  
Clinical Chemistry ◽  
Laboratory Medicine ◽  
International Federation ◽  
Pars Intermedia ◽  
Klinische Relevanz

ZusammenfassungZiel dieser Arbeit war, den Einsatz des Automaten IMMULITE 2000® mit einem immunometrischen Chemolumineszenz-Assay für die Bestimmung von adrenokortikotropem Hormon (ACTH), Insulin und Insulinwachstumsfaktor 1 (IGF-1) zu prüfen und entsprechende Referenzbereiche für Ponys zu berechnen. Material und Methoden: Nachmittags gewonnene Blutproben von 130 Ponys im Alter von 3–32 Jahren wurden auf Insulin, IGF-1 und ACTH untersucht. Die Referenzbereiche wurden nach der Richtlinie EP28-A3C der International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) aus dem Jahr 2010 berechnet. Ergebnisse: Die Präzision für die Messungen an einem Tag lag für Insulin im Mittel bei 3,28%, für IGF-1 bei 1,84% und für ACTH bei 3,35%. Die Tag-zu-Tag-Präzision (Insulin: 3,45%; IGF-1: 2,89%; ACTH: 9,77%) wurde an drei aufeinanderfolgenden Tagen gemessen, wobei die Lagerung der Proben bei 4 °C erfolgte. Es zeigte sich kein signifikanter Verlust der Insulinaktivität bzw. der Konzentrationen von ACTH und IGF-1. Für Insulin und IGF-1 ergaben sich altersunabhängige Referenzbereiche (Insulin: 2,0–21,2 mU/l; IGF-1: 50,2–357,2 μg/l). Dagegen wurde für ACTH ein altersabhängiger Referenzbereich er - mittelt, der bei Ponys im Alter von 3–12 Jahren signifikant niedriger war (4,2–19,8 pg/ml) als bei Ponys im Alter von 13–32 Jahren (5,0–22,6 pg/ml). Schlussfolgerung: Das verwendete Analyseverfahren eignet sich für die Untersuchung von Serumproben von Ponys auf ACTH, IGF-1 und Insulin, doch wird der Einsatz bei der Insulinmessung durch die untere Nachweisgrenze von 2,0 mU/l limitiert. Klinische Relevanz: Die berechneten Referenzbereiche für Insulin und ACTH sind hilfreich für die Diagnostik und Verlaufskontrolle des equinen metabolischen Syndroms und der Pituitary Pars Intermedia Dysfunction (PPID).

Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed to cadmium and/or alcohol

2019 ◽  
Vol 35 (4) ◽  
pp. 277-293 ◽  
Author(s):  
Iwona Markiewicz-Górka ◽  
Piotr Kuropka ◽  
Lidia Januszewska ◽  
Aleksandra Jaremków ◽  
Paweł Pawłowski ◽  
...  
Keyword(s):  
Physical Training ◽  
Type I Collagen ◽  
Morphological Changes ◽  
Female Rats ◽  
Control Group ◽  
Bone Resorption Marker ◽  
Type I ◽  
Mineral Contents ◽  
Morphological Alterations ◽  
Bone Parameters

The aim of the study was to investigate the effect of physical training on bone parameters of rats exposed to alcohol (Al) and/or cadmium (Cd). Young female rats were divided into one control group and six groups exposed to Cd and/or Al. Al (36% calories of diet) and Cd (20 mg Cd/kg feed) were administered with liquid diet. Half of the rats from the treated groups were subjected to treadmill training (20 m/min for 0.5 h, 4 days a week). The experiment was carried out for 5 months. Al decreased the concentration of calcium (Ca) and iron (Fe) in the femur, whereas Cd and Cd + Al intake reduced the contents of Ca, Fe and zinc. Al and/or Cd caused an increase in both C-terminal telopeptide of type I collagen (CTX1; bone resorption marker) and osteocalcin (OC; formation indicator) and enhanced the degree of porosity and flexural strength of the femur. Al partially prevented the loss of Fe from the bone caused by Cd, but intensified the inhibition of growth of body weight in comparison with separate exposure to Cd. In rats co-exposed to Cd + Al, the levels of CTX1 were greater compared with those treated with Al or Cd separately, and the density was less than that in rats exposed to Al separately. The training caused increases of magnesium and Ca contents, decreases in CTX1, as well as increases in OC and bone density, decreasing their porosity. The effect of training on the bone status, however, was limited (especially in rats co-exposed to Cd and Al) because of the increase in their mineralization, stimulated by exercises, was insufficient in relation to collagen production intensity. In conclusion, training had favourable effects on some bone parameters, but did not compensate for the negative effects of Al and/or Cd exposure on the poor mineralization and histopathological and morphological changes in the femur.

scholarly journals Introducing the new laboratory standard of HbA1c determination in Hungary

2011 ◽  
Vol 152 (14) ◽  
pp. 555-558
Author(s):  
Gabriella Bekő
Keyword(s):  
Master Equation ◽  
Clinical Chemistry ◽  
Reference Method ◽  
Laboratory Medicine ◽  
International Federation ◽  
New Method ◽  
Interlaboratory Comparisons

A new laboratory standard, specific for HbA1c was prepared by the International Federation of Clinical Chemistry and Laboratory Medicine. Consequently, manufacturers will supply their calibrators with the International Federation of Clinical Chemistry and Laboratory Medicine standard. Laboratories in Hungary switch to this new method in April 1, 2011. After this date, results of HbA1c measurements will be reported in International Federation of Clinical Chemistry and Laboratory Medicine units (mmol/mol) and in the derived National Glycohemoglobin Standardization Program units (%) calculated by master equation from the International Federation of Clinical Chemistry and Laboratory Medicine/National Glycohemoglobin Standardization Program methods. Using the new standardization the HbA1c measurements will be traceable to the International Federation of Clinical Chemistry and Laboratory Medicine reference method and interlaboratory comparisons will be possible. Orv. Hetil., 2011, 152, 555–558.

scholarly journals The Role of PINP in Diagnosis and Management of Metabolic Bone Disease

2021 ◽  
Vol 42 (1) ◽  
pp. 3-10
Author(s):  
Melissa Gillet ◽  
Samuel Vasikaran ◽  
Charles Inderjeeth
Keyword(s):  
Fracture Risk ◽  
Bone Disease ◽  
Type I Collagen ◽  
Metabolic Bone Disease ◽  
Bisphosphonate Therapy ◽  
Response To Therapy ◽  
Type I ◽  
Reference Marker ◽  
Monitoring Therapy ◽  
Metabolic Bone

Abstract Serum procollagen type I N-propeptide (PINP) is designated the reference marker of bone formation in osteoporosis; the reference marker for resorption is C-terminal telopeptide of type I collagen (CTX). PINP has very low circadian and biological variation, is not affected by food intake, and is very stable in serum after venepuncture. The two automated commercial assays for PINP provide similar results in subjects with normal renal function, allowing reference intervals to be used interchangeably. Bone turnover markers (BTM) are currently not recommended for fracture risk assessment and therefore not included in fracture risk calculators. In the management of osteoporosis, the main utility of BTM including PINP is for monitoring therapy, both antiresorptive as well as anabolic agents; monitoring is thought to help improve adherence. PINP as well as CTX may also be used in assessing offset of drug action following a pause in bisphosphonate therapy, to help decide when to re-instate therapy, or following cessation of denosumab therapy to assess efficacy of follow-on bisphosphonate therapy. PINP may also be used in the diagnosis of Paget’s disease of bone as well as in monitoring response to therapy and for recurrence. Although BTM other than bone alkaline phosphatase are currently not recommended for use in metabolic bone disease of chronic kidney disease, PINP measured by assays specific to the intact molecule has potential in this condition. Further studies are needed to examine this area, as well as in malignant bone disease.

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