Preemptive NUDT15 genotyping: redefining the management of patients with thiopurine-induced toxicity
AbstractBackground:Thiopurine methyltransferase (TPMT) gene variants have achieved limited success in predicting the outcome of thiopurine therapy, which shows wide inter-individual variations. The literature indicates a strong association between theNUDT15gene variant and thiopurine-induced toxicity in Asian patients. The present study intends to explore the role of theNUDT15variant (C415T) in Indian patients on thiopurine therapy.Methods:NUDT15andTPMTgenotyping were performed using amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) and the restriction fragment length polymorphism (RFLP) technique.Results:Of 370 samples received forTPMTtesting, 206 samples were available forNUDT15genotyping. TheNUDT15risk allele frequency was 10.7%, with the frequency of wild, heterozygous and mutant genotypes being 80.6%, 17.5% and 1.9%, respectively.TPMTvariants were seen in 13 of 370 (3.5%) patients, whereas theNUDT15variant was seen in 40 of 206 (19.4%) patients. Thiopurine-induced toxicity information was available for 101 patients, among whom 10 developed leukopenia and all harbored theNUDT15variant (p<0.0001).NUDT15was clinically more relevant thanTPMTin terms of sensitivity and specificity, as well as with a statistically significant difference in thiopurine dose requirement for patients with theNUDT15variant.Conclusions:A preemptiveNUDT15genotyping approach can therefore help identify high-risk patients (NUDT15C415T positive) who could benefit from thiopurine dose reduction, thereby preventing fatal thiopurine-induced toxicity.