Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes

Holzforschung ◽  
2012 ◽  
Vol 66 (3) ◽  
Author(s):  
Zhengyu Shu ◽  
Jiguang Wu ◽  
De Chen ◽  
Lanxing Cheng ◽  
Yi Zheng ◽  
...  
Keyword(s):  
Tween 80 ◽  
Pinus Massoniana ◽  
Fermentation Medium ◽  
Lipase Production ◽  
Thermomechanical Pulp ◽  
Pitch Control ◽  
Thermomechanical Pulping ◽  
Initial Ph ◽  
Inoculum Volume ◽  
Optimized Conditions

AbstractProduction and process optimization of a thermostable lipase fromBurkholderiasp. ZYB002 and the application potential of this lipase for pitch control of thermomechanical pulp (TMP) has been investigated. The optimized physical and nutritional factors ofBurkholderiasp. ZYB002 were found to be (all units in%, w v-1): 0.5 peptone, 0.025 urea, 0.5 glycerol, 0.1 NaHCO3, 0.2 K2HPO4, 1.5 Tween 80, 1.1 olive oil, four inoculum volume, and initial pH 8.5. The lipase production process was carried out at 30°C at 250 rpm for 36 h in 250 ml Erlenmeyer flasks containing 35 ml fermentation medium and five glass beads. Under the optimized conditions, lipase activity reached 216.5 U ml-1. In a TMP ofPinus massoniana, 39% of triglycerides were hydrolyzed by the lipase under optimized conditions [pulp concentration 6% (w v-1), lipase dose 20 U g-1based on dry TMP, pulp initial pH 7.5, and 65°C for 30 min]. The lipase in question displayed a great potential for pitch control of TMP.

scholarly journals Improvement of lipase production from Geotrichum sp. in shaken flasks

2012 ◽  
Vol 18 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Resende Maldonadoa ◽  
Luiz Pancieraa ◽  
Alves Macedob ◽  
Marcio Mazuttic ◽  
Francisco Maugeria ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Lipase Activity ◽  
Corn Steep Liquor ◽  
Lipase Production ◽  
Inoculum Size ◽  
Inoculum Age ◽  
Initial Ph ◽  
Oil Concentration ◽  
Inoculum Volume ◽  
Steep Liquor

This work is focused on the study of different variables on inoculum build-up aiming to improve the lipase production by Geotrichum sp. by means a sequential strategy of experimental design. The effects of inoculum size, corn steep liquor concentration, volume of inoculum, pH of medium, age of inoculum and soybean oil concentration on lipase activity were assessed by means of two factorial experimental designs. A maximum lipase activity of 35.20?0.8 U/mL was obtained with a inoculum composed of one circular area of 0.78cm2 containing spores, 50 mL of inoculum volume medium, 12 hours of inoculum age, 15% w/v of corn steep liquor concentration, 1.0%w/v of soybean oil concentration and initial pH 5.0 at 30?C and 150 rpm in flasks. This work showed that an enhancement of lipase activity can be obtained using a sequential statistical factorial approach to define the variables for inoculum build-up.

Optimization of Lipase Production from Arthrobacter sp.SD5

2013 ◽  
Vol 791-793 ◽  
pp. 116-119 ◽  
Author(s):  
Peng Ren ◽  
Wei Hua Lin
Keyword(s):  
Carbon Source ◽  
Culture Condition ◽  
Ph Value ◽  
Tween 80 ◽  
Lipase Production ◽  
Medium Composition ◽  
Liquid Volume ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Ph

Lipase is a kind of important hydrolase. It was widely used in the fields of food, leather, detergent and pharmaceutical. The production of lipase from Arthrobacter sp. SD5 was studied in the present paper. The medium composition and culture condition were optimized in order to improve lipase production. The results showed the best optimum conditions were on the following: culture temperature (40°C); initial pH value (8.0); liquid volume (20%); carbon source (olive oil, 2.5%); nitrogen source (peptone, 1.0%); biosurfactant (Tween-80, 0.2%).

scholarly journals Artificial Neural Networks and Response Surface Methodology Approach for Optimization of an Eco-Friendly and Detergent-Stable Lipase Production from Actinomadura Keratinilytica Strain Cpt29

2021 ◽  
Vol 68 (3) ◽  
pp. 575-586
Author(s):  
Noura Semache ◽  
Fatiha Benamia ◽  
Bilal Kerouaz ◽  
Inès Belhaj ◽  
Selma Bounour ◽  
...  
Keyword(s):  
Incubation Temperature ◽  
Tween 80 ◽  
Fold Increase ◽  
Data Fitting ◽  
Lipase Production ◽  
Potential Candidate ◽  
Cultivation Time ◽  
North East ◽  
Lipase A ◽  
Commercial Laundry

This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt29 isolated from poultry compost in north east of Algeria, for use in detergent industries. AKL29 shows a significant lipase activity (45 U/mL) towards hydrolyzed triacylglycerols, indicating that it is a true lipase. For maximum lipase production the modeling and optimization of potential culture parameters such as incubation temperature, cultivation time, and Tween 80 (v/v) were built using RSM and ANN approaches. The results show that both the two models provided good quality predictions, yet the ANN showed a clear superiority over RSM for both data fitting and estimation capabilities. A 4.1-fold increase in lipase production was recorded under the following optimal condition: incubation temperature (37.9 °C), cultivation time (111 h), and Tween 80 (3.27%, v/v). Furthermore, the partially purified lipase showed good stability, high compatibility, and significant wash performance with various commercial laundry detergents, making this novel lipase a promising potential candidate for detergent industries.

scholarly journals Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: the process comparison analysis by statistical approach

2013 ◽  
Vol 67 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Sonja Jakovetic ◽  
Zorica Knezevic-Jugovic ◽  
Sanja Grbavcic ◽  
Dejan Bezbradica ◽  
Natasa Avramovic ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lipase Activity ◽  
Sunflower Oil ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Lipase Production ◽  
Fermentation Time ◽  
Process Comparison ◽  
Central Composite Experimental Design ◽  
Oil Concentration

Pseudomonas aeruginosa was repeatedly reported as powerful producer of rhamnolipid biosurfactants as well as producer of hydrolytic enzymes. In this study effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. Investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween? 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration 0.8 %, Tween? 80 concentration 0.05 %, temperature 30?C, and fermentation time 72 h) and 11111 IU dm-3(sunflower concentration of 0.4 %, Tween? 80 concentration of 0.05 %, temperature of 30?C, and fermentation time of 120 h), respectively.

A Novel Strain Pseudomonas Aeruginosa CS-2 Producing an Organic Solvent-Tolerant Lipase

2011 ◽  
Vol 343-344 ◽  
pp. 774-780
Author(s):  
Ren Peng ◽  
Jin Ping Lin ◽  
Dong Zhi Wei
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Organic Solvent ◽  
Gum Arabic ◽  
Lipase Production ◽  
16S Rdna Sequence ◽  
Organic Solvent Tolerant ◽  
Initial Ph ◽  
Improved Stability ◽  
Solvent Tolerant ◽  
Physiological And Biochemical

Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified asPseudomonas aeruginosaCS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform,n-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO40.5%, MgSO4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h.

Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation

2018 ◽  
Vol 43 (3) ◽  
pp. 240-247 ◽  
Author(s):  
Nurullah Akcan
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Bacillus Licheniformis ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Inoculum Level ◽  
Cultural Conditions ◽  
Initial Ph ◽  
Fermentation Parameters

AbstractObjective:The aim of this work was to study the optimal cultivation conditions for β-galactosidase production byBacillus licheniformisATCC 12759.Materials and methods:The screening of β-galactosidase production fromB. licheniformisATCC 12759 was performed by solid state fermentation method on media rich with rice bran (RB). Different factors were tested for the optimization of β-galactosidase production.Results:Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, moisture content, initial pH, agitation speed, size of fermentation medium and optimum temperature of β-galactosidase activity were studied separately. Maximal amount of β-galactosidase production was obtained when solid-state fermentation (SSF) was carried out using RB, having inoculum level 35%, moisture content of 20%, initial pH 7.5 at 37°C for 48 h.Conclusion:Results indicated that optimal fermentation conditions play a key role in the maximum production of β-galactosidase fromB. licheniformisATCC 12759. This study shows the potential of the studied enzymes to be promoting candidates for the degradation of lactose and production of important bioproducts.

Effect of triglycerides on the production of lipids and lipase by Mucor hiemalis

1983 ◽  
Vol 29 (6) ◽  
pp. 664-669 ◽  
Author(s):  
M. Waheed Akhtar ◽  
A. Qayyum Mirza ◽  
M. Nadeem Nawazish ◽  
M. I. D. Chughtai
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Growth Phase ◽  
Lipase Activity ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Lipase Production ◽  
Mucor Hiemalis ◽  
Sterol Esters ◽  
Ethanolamine Phosphatidyl

The effect of triglycerides on the growth of Mucor hiemalis and the production of lipase and mycelial lipids was studied. Addition of 1% triglycerides to the fermentation medium was best for the mycelial as well as the broth lipase production. The added triglycerides seemed to be utilized through the formation of free fatty acids, and towards the end of the growth phase most of the triglycerides and their hydrolysis products were utilized. The mycelial lipase activity was maximum (66 U/g dry mycelium) at the end of the growth phase, while the maximal broth lipase activity (204 U/100 mL) was achieved after the cell lysis had started. The lipids produced per gram mycelia were high initially (260 mg/g dry weight at 48 h), reducing gradually later. With increase in growth the maximum mycelial lipids per 100 mL of culture medium was obtained after 96 h (176 mg/100 mL). The various fractions detected in the mycelial lipid extracts were sterol esters, triglycerides, free fatty acids, diglycerides, sterols, monoglycerides, phosphatidyl ethanolamine, phosphatidyl choline, and small amounts of an unknown polar lipid at all the stages of fermentation studied. Proportion between total neutral and total polar lipids remained nearly constant throughout fermentation.

Isolation and Degradation Characterization of Trichloroethylene-Degrading Bacteria

2012 ◽  
Vol 610-613 ◽  
pp. 3136-3139
Author(s):  
Hui Xing Liang
Keyword(s):  
Fermentation Medium ◽  
Chlorinated Organic Compounds ◽  
Degradation Temperature ◽  
Degrading Bacteria ◽  
Initial Ph ◽  
Chlorinated Organic ◽  
Bacterium Strain ◽  
Rotary Speed ◽  
Degradation Time

Trichloroethylene is one of chlorinated organic compounds widely used as a solvent and degreasing agent in industry. Because of uninformed disposal in the past, trichloroethylene has become one of major contaminant in environment, and this situation has brought about a serious public concern for its toxicity. A promising approach to solving this problem is bioremediation using degrading-bacteria. A bacterium(strain TC-1) was isolated from environment, which could degrade trichloroethylene. It was preliminary identified as the genus of Sporosarcina sp.. The results showed that the optimal degradation temperature, degradation time, rotary speed and the initial pH of fermentation medium were 25°C, 60 h, 180 rpm and 7.5 respectively, the ratio of degradation reached 95.56% under this conditions.

Optimization of Medium Components for β-Glucosidase Production from Aspergillus Niger by Response Surface Methodology

2013 ◽  
Vol 419 ◽  
pp. 328-333 ◽  
Author(s):  
Chao Zhang ◽  
Rui Huang ◽  
Hui Tian ◽  
Ru Ming Zhao ◽  
Fa Shun Yu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Influence Factors ◽  
Mutual Effect ◽  
Tween 80 ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Malt Extract ◽  
Medium Components

β-Glucosidase is the key enzyme for the utilization of lignocellulose.But the commercial β-glucosidase can’t be produced. This paper focuses on the study of the β-glucosidase fermentation process.The fermentation medium components for β-glucosidase production from Aspergil lusniger was optimized by response surface methodology (RSM). Firstly, the three of the most important influence factors yeast extract, MnSO4•H2O and MgSO4•7H2O was obtained from Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration and mutual effect of three factors were predicted by RSM. The results showed that the best medium composition was Malt extract 18g/L, Yeast extract 3.22g/L, KH2PO4 3g/L, MnSO4•H2O 0.58mM, Tween-80 0.5mL/L and MgSO4•7H2O 0.23g/L. Under these fermentation conditions, the activity of β-glucosidase was up to 7.33IU/mL with increasing 23.2% than before.

Monitoring Initial Glucose Concentration for Optimum pH Control during Fermentation of Microbial Cellulose in Rotary Discs Reactor

2013 ◽  
Vol 594-595 ◽  
pp. 319-324 ◽  
Author(s):  
Khairul Azly Zahan ◽  
Norhayati Pa’e ◽  
Kok Fook Seng ◽  
Ida Idayu Muhamad
Keyword(s):  
Glucose Concentration ◽  
Ph Control ◽  
Fermentation Medium ◽  
Initial Glucose Concentration ◽  
Cellulose Production ◽  
Microbial Cellulose ◽  
Cellulose Yield ◽  
Optimum Ph ◽  
Initial Ph ◽  
Wet Weight

The study aimed to investigate the effect of initial glucose concentration on the microbial cellulose production using Acetobacter xylinum in a Rotary Discs Reactor (RDR-2 liter volume). The fermentations were carried out for four days at temperature 28°C, initial pH 6.5, and 9 rpm of rotation speed; meanwhile, the initial glucose concentration was manipulated in the range of 0.5-5.0 % (w/v). The cell growth was stimulated using 1.4% (v/v) ethanol in the fermentation medium. The result indicated that 1% (w/v) of initial glucose concentration provided the highest microbial cellulose yield with total wet weight of 296.1657g/l. The increase of initial glucose concentration resulted to the decrease of microbial cellulose yield and greater pH drop after fermentation. It can be conclude that production of microbial cellulose using RDR could produce relatively much higher microbial cellulose with less amounts of glucose in a shorter fermentation period compared to static fermentation due to more efficient oxygen uptake during rotary movements and homogenous environment for microbial growth.

Sign in / Sign up

Export Citation Format

Share Document