Artificial Neural Networks and Response Surface Methodology Approach for Optimization of an Eco-Friendly and Detergent-Stable Lipase Production from Actinomadura Keratinilytica Strain Cpt29

This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt29 isolated from poultry compost in north east of Algeria, for use in detergent industries. AKL29 shows a significant lipase activity (45 U/mL) towards hydrolyzed triacylglycerols, indicating that it is a true lipase. For maximum lipase production the modeling and optimization of potential culture parameters such as incubation temperature, cultivation time, and Tween 80 (v/v) were built using RSM and ANN approaches. The results show that both the two models provided good quality predictions, yet the ANN showed a clear superiority over RSM for both data fitting and estimation capabilities. A 4.1-fold increase in lipase production was recorded under the following optimal condition: incubation temperature (37.9 °C), cultivation time (111 h), and Tween 80 (3.27%, v/v). Furthermore, the partially purified lipase showed good stability, high compatibility, and significant wash performance with various commercial laundry detergents, making this novel lipase a promising potential candidate for detergent industries.

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New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1186/s43088-020-00090-8 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Mohamed A. Mohamed ◽

Hassan M. Awad

Keyword(s):

Large Scale ◽

Linseed Oil ◽

Tween 80 ◽

Fold Increase ◽

Lipase Production ◽

Medium Composition ◽

Scale Production ◽

Wide Ph Range ◽

Wadi El Natrun ◽

Ph Range

Abstract Background Bioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications. Halo-alkaline marshes in Wadi El Natrun in Egypt are some of the most stable ecological systems in the world, and because of the double extremities of alkalinity and salinity, they harbor individual microbes capable of adapting stress conditions. Results Eight strains were recovered from the coastline soil of Al-Beida Lake in Wadi El Natrun and have been tested for lipase production. Among the eight isolates, the strain SBLWN_MH2 was the most active producer of lipase (7.5 U/ml). The crude SBLWN_MH2 lipase showed activity over a wide pH range (3.5 to 13) with an optimum pH at 10.5, and it was able to show more than 75% of its highest activity at pH elevated up to 13. The identification using phenotypic and genotypic methods strongly indicated that the strain SBLWN_MH2 belonged to the genus Streptomyces with a similarity of 99%. Thus, it has been given the suggested name Streptomyces sp. SBLWN_MH2 (MG593538). SBLWN_MH2 produced extracellular lipase in modified starch casein medium supplemented with different oils or Tween-80, and the potential production rate has been attained in the case of linseed oil after 3 days. Further experiments have been carried out to optimize medium composition through Box-Behnken design and response surface methodology, and it was possible to achieve more than 3.5-fold increase in lipase production. Conclusions The present study indicates that Streptomyces sp. SBLWN_MH2 is a potential lipase producer and could be fruitfully employed in the large-scale production of highly alkaline lipase.

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Study of the Activity of 3-benzyl-5-(4-chloro-arylazo)-4-thioxo-imidazolidin-2-one against Schistosomiasis Mansoni in Mice

The Scientific World JOURNAL ◽

10.1100/2012/520524 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Andréa Cristina Apolinário da Silva ◽

Juliana Kelle de Andrade Lemoine Neves ◽

João Inácio Irmão ◽

Vláudia Maria Assis Costa ◽

Valdênia Maria Oliveira Souza ◽

...

Keyword(s):

Specific Antigen ◽

Tween 80 ◽

Histopathological Analysis ◽

Second Phase ◽

No Production ◽

Potential Candidate ◽

Treatment Regimens ◽

Morphological Aspects ◽

Oil Water

Previous studies conducted with the imidazolidinic derivative 3-benzyl-5-(4-chloro-arylazo)-4-thioxo-imidazolidin-2-one (LPSF-PT05) show outstanding activity against adultSchistosoma mansoniwormsin vitro. In the first phase of this study,S. mansoni-infected mice were treated, orally, with 100 mg/Kg of the LPSF-PT05 in three formulations: Tween 80 and saline solution, oil/water (70 : 30) emulsion, and solid dispersion with polyethylene glycol (PEG). In the second phase, three other doses of the LPSF-PT05 in PEG were tested: 3, 10, 30 mg/kg. These treatment regimens significantly reduced the number of recovered worms due to increases in the solubility of the compound in this formulation; the greatest reduction (70.5%) was observed at the dose of 100 mg/kg. There was no changes in the pattern of mature egg compared to immature eggs; however there was a significant increase in the number of dead eggs. Histopathological analysis of liver tissue showed changes in morphological aspects of the hepatic parenchyma with decrease exudative-productive hepatic granuloma stages, although we found no significant differences in IFN-γ, IL-4, IL-10, or NO production in response to the specific antigen SEA. The results show the derivative LPSF-PT05 to be a potential candidate in the etiological treatment of schistosomiasis with a possible dampening effect of the granulomatous process.

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Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: the process comparison analysis by statistical approach

Hemijska industrija ◽

10.2298/hemind121008114j ◽

2013 ◽

Vol 67 (4) ◽

pp. 677-685 ◽

Cited By ~ 3

Author(s):

Sonja Jakovetic ◽

Zorica Knezevic-Jugovic ◽

Sanja Grbavcic ◽

Dejan Bezbradica ◽

Natasa Avramovic ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Lipase Activity ◽

Sunflower Oil ◽

Hydrolytic Enzymes ◽

Tween 80 ◽

Lipase Production ◽

Fermentation Time ◽

Process Comparison ◽

Central Composite Experimental Design ◽

Oil Concentration

Pseudomonas aeruginosa was repeatedly reported as powerful producer of rhamnolipid biosurfactants as well as producer of hydrolytic enzymes. In this study effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. Investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween? 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration 0.8 %, Tween? 80 concentration 0.05 %, temperature 30?C, and fermentation time 72 h) and 11111 IU dm-3(sunflower concentration of 0.4 %, Tween? 80 concentration of 0.05 %, temperature of 30?C, and fermentation time of 120 h), respectively.

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Growth of Staphylococcus aureus in Milks and Creams with Various Amounts of Milk Fat

Journal of Food Protection ◽

10.4315/0362-028x-52.8.540 ◽

1989 ◽

Vol 52 (8) ◽

pp. 540-543 ◽

Cited By ~ 6

Author(s):

MARGARET I. HALPIN-DOHNALEK ◽

ELMER H. MARTH

Keyword(s):

Staphylococcus Aureus ◽

Milk Fat ◽

Incubation Temperature ◽

Skim Milk ◽

Fold Increase ◽

Fat Content ◽

Strain Variation ◽

High Fat ◽

Whole Milk

Staphylococcus aureus strains 100-A, 196-E, 254, 473, 505, and 521 generally grew better in skim milk and whole milk (3.5% milk fat) than in light cream (18% milk fat) and heavy whipping cream (36% milk fat) at 25 and 37°C. S. aureus strains grew to greater than 106/ml from an inoculum of ca. 103/ml in skim and whole milk held at 37°C. From the same level of inoculum, strains grew to 106–107/ml in light cream held at 37°C. A 10 to 100-fold increase in population was observed for most strains in heavy whipping cream inoculated with ca. 104 S. aureus/ml and held at 37°C. Less than a 10-fold increase in population was noted for strain 254 in heavy whipping cream held at 37°C. When strains of S. aureus were inoculated into milks and creams and held at 25°C, populations of the pathogen increased approximately 100-fold in skim and whole milk and 10-fold or less in light cream and heavy whipping cream. S. aureus was enumerated using Chapman Stone, Mannitol Salt, Baird-Parker, and Vogel-Johnson agars; best results were obtained with Baird-Parker and Vogel-Johnson agars, particularly when high-fat fluid products were tested. Milk fat content, incubation temperature, and strain variation influenced the ability of S. aureus to grow in various dairy fluids.

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Feather Keratin Hydrolysis by an Aquatic Bacterium Meiothermus I40 from Hot Spring Water

International Journal of Food Engineering ◽

10.2202/1556-3758.2201 ◽

2011 ◽

Vol 7 (2) ◽

Cited By ~ 1

Author(s):

Jing-Iong Yang ◽

Jen-Min Kuo ◽

Wen-Ming Chen ◽

Huei-Jing Ke ◽

Yi-Ju Chou

Keyword(s):

Yeast Extract ◽

Incubation Temperature ◽

Hot Spring ◽

Fold Increase ◽

Fermentation Conditions ◽

Effective Factors ◽

Extract Concentration ◽

Initial Stage ◽

Keratin Hydrolysis ◽

Fermentation Parameters

After undergoing keratinase digestion, feather wastes could have a great potential as a source of proteins and amino acids for many applications. In this study, the fermentation conditions of feather-degrading Meiothermus sp. strain I40 (I40) were optimized to enhance the biodegradation of chicken feather to hydrolysates. Initially, the factors essential for I40 keratinase production in submerged fermentation were screened, whereas response surface methodology (RSM) was then employed to evaluate the interactions among the effective factors. At first stage, eight fermentation parameters were screened using a Plackett-Burman (PB) design. Four effective factors identified by PB screening, namely feather concentration, tryptone concentration, yeast extract concentration, and incubation temperature, were further investigated their effects on keratinase production by RSM using central composite design (CCD). The I40 fermentation conditions for maximal keratinase activity were as follows: tryptone concentration 0.16 percent (w/v), yeast extract concentration 0.27 percent (w/v), feather concentration 0.08 percent (w/v), and incubation temperature at 51.7°C for 72 hr under 120 rev/min shaking. Compared to the initial stage, a 13.3-fold increase in keratinase activity was achieved when I40 incubated in the optimized conditions.

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Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

10.21203/rs.2.11777/v1 ◽

2019 ◽

Author(s):

Ameni KTATA ◽

karray aida ◽

Ines Mnif ◽

Sofiane BEZZINE

Keyword(s):

Waste Water ◽

Response Surface Methodology ◽

Enzyme Activity ◽

Lipase Production ◽

Maximum Activity ◽

Medium Composition ◽

P Value ◽

Potential Candidate ◽

Box Behnken Design ◽

Optimized Conditions

Abstract Background Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD. The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0,3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions A 6,68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/ml. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.

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Effect of fermentation conditions on lipase production by Candida utilis

Journal of the Serbian Chemical Society ◽

10.2298/jsc0709757g ◽

2007 ◽

Vol 72 (8-9) ◽

pp. 757-765 ◽

Cited By ~ 14

Author(s):

Sanja Grbavcic ◽

Suzana Dimitrijevic-Brankovic ◽

Dejan Bezbradica ◽

Slavica Siler-Marinkovic ◽

Zorica Knezevic

Keyword(s):

Candida Utilis ◽

Lipolytic Activity ◽

Nitrogen Sources ◽

Tween 80 ◽

Lipase Production ◽

Malt Extract ◽

Fermentation Conditions ◽

Carbon And Nitrogen ◽

Wild Yeast ◽

Negative Effect

A wild yeast strain isolated from spoiled soybean oil and identified as Candida utilis initially presented rather low lipase activity (approximately 4 IU dm-3) in submerged culture in a universal yeast medium containing 2 % malt extract. Studies were undertaken to improve the lipase production. The best yields of lipase were obtained with a medium supplemented with caprylic and oleic acids as inducers, but higher concentrations of the former (> 0.5 %) had a negative effect on the lipase production and cell growth. The type of nitrogen source seemed also to be very important. The highest lipolytic activity of 284 IU dm-3 was achieved after 5 days of fermentation in a medium containing oleic acid and hydrolyzed casein as carbon and nitrogen sources, respectively, and supplemented with Tween 80?. It was shown that optimization of the fermentation conditions can lead to a significant improvement in the lipase production (more than 70-fold higher compared to the initial value obtained in the non-optimized medium). .

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Rv1218c, an ABC Transporter of Mycobacterium tuberculosis with Implications in Drug Discovery

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00610-10 ◽

2010 ◽

Vol 54 (12) ◽

pp. 5167-5172 ◽

Cited By ~ 47

Author(s):

Meenakshi Balganesh ◽

Sanjana Kuruppath ◽

Nimi Marcel ◽

Sreevalli Sharma ◽

Anju Nair ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Drug Discovery ◽

Bacterial Infections ◽

Fold Increase ◽

Potential Candidate ◽

Multicopy Plasmid ◽

Wild Type ◽

Experimental Drugs ◽

Physiological Relevance ◽

In The Wild

ABSTRACT Efflux systems are important in determining the efficacy of antibiotics used in the treatment of bacterial infections. In the last decade much attention has been paid to studying the efflux pumps of mycobacteria. New classes of compounds are under investigation for development into potential candidate drugs for the treatment of tuberculosis. Quite often, these have poor bactericidal activities but exhibit excellent target (biochemical) inhibition. Microarray studies conducted in our laboratories for deciphering the mode of action of experimental drugs revealed the presence of putative ABC transporters. Among these transporters, Rv1218c was chosen for studying its physiological relevance in mediating efflux in Mycobacterium tuberculosis. A ΔRv1218c mutant of M. tuberculosis displayed a 4- to 8-fold increase in the inhibitory and bactericidal potency for different classes of compounds. The MICs and MBCs were reversed to wild-type values when the full-length Rv1218c gene was reintroduced into the ΔRv1218c mutant on a multicopy plasmid. Most of the compound classes had significantly better bactericidal activity in the ΔRv1218c mutant than in the wild-type H37Rv, suggesting the involvement of Rv1218c gene product in effluxing these compounds from M. tuberculosis. The implication of these findings on tuberculosis drug discovery is discussed.

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Enhanced Oral Bioavailability of Efavirenz by Solid Lipid Nanoparticles:In VitroDrug Release and Pharmacokinetics Studies

BioMed Research International ◽

10.1155/2014/363404 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 43

Author(s):

Praveen Kumar Gaur ◽

Shikha Mishra ◽

Meenakshi Bajpai ◽

Anushika Mishra

Keyword(s):

Drug Release ◽

Peak Plasma ◽

Peak Plasma Concentration ◽

Tween 80 ◽

Fold Increase ◽

Pharmacokinetic Profile ◽

Water Soluble ◽

Physical Parameters ◽

Solid Lipid

Solid lipid nanoparticle is an efficient lipid based drug delivery system which can enhance the bioavailability of poorly water soluble drugs. Efavirenz is a highly lipophilic drug from nonnucleoside inhibitor category for treatment of HIV. Present work illustrates development of an SLN formulation for Efavirenz with increased bioavailability. At first, suitable lipid component and surfactant were chosen. SLNs were prepared and analyzed for physical parameters, stability, and pharmacokinetic profile. Efavirenz loaded SLNs were formulated using Glyceryl monostearate as main lipid and Tween 80 as surfactant. ESLN-3 has shown mean particle size of124.5±3.2nm with a PDI value of 0.234, negative zeta potential, and 86% drug entrapment.In vitrodrug release study has shown 60.6–98.22% drug release in 24 h by various SLN formulations. Optimized SLNs have shown good stability at 40°C±2°C and75±5% relative humidity (RH) for 180 days. ESLN-3 exhibited 5.32-fold increase in peak plasma concentration (Cmax⁡) and 10.98-fold increase in AUC in comparison to Efavirenz suspension (ES).

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Understorey light environments in a north-east Australian rain forest before and after a tropical cyclone

Journal of Tropical Ecology ◽

10.1017/s0266467400006453 ◽

1992 ◽

Vol 8 (3) ◽

pp. 241-252 ◽

Cited By ~ 29

Author(s):

S. M. Turton

Keyword(s):

Tropical Cyclone ◽

Rain Forest ◽

Light Availability ◽

Fold Increase ◽

Canopy Openness ◽

Tree Seedlings ◽

Site Factors ◽

North East ◽

Canopy Disturbance ◽

Before And After

ABSTRACTThis paper describes estimates of canopy openness and associated light availability in the understorey of a north-east Australian rain forest before and immediately after a tropical cyclone. On the basis of 20 hemispherical (fisheye) canopy photographs it was shown that direct, diffuse and total site factors increased significantly as a result of the slight-to-moderate canopy disturbance caused by the cyclone. In the understorey, median total site factors ranged from 2.5–3.4% before the cyclone and from 6.0–8.6% after the cyclone, representing a 2- to 3-fold increase in potential light availability. Following the cyclone, mean relative gap frequencies increased substantially at all altitudes but particularly at canopy positions more than 70° above the horizon. Cyclone-induced canopy disturbance not only reduced the complexity of the understorey light regime but may have also increased the seasonal variability of light within the understorey of the forest during the interval of canopy recovery. The implications of these results for the ecophysiology of understorey tree seedlings and saplings at several temporal scales are discussed.

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