Optimization of Lipase Production from Arthrobacter sp.SD5

2013 ◽  
Vol 791-793 ◽  
pp. 116-119 ◽  
Author(s):  
Peng Ren ◽  
Wei Hua Lin
Keyword(s):  
Carbon Source ◽  
Culture Condition ◽  
Ph Value ◽  
Tween 80 ◽  
Lipase Production ◽  
Medium Composition ◽  
Liquid Volume ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Ph

Lipase is a kind of important hydrolase. It was widely used in the fields of food, leather, detergent and pharmaceutical. The production of lipase from Arthrobacter sp. SD5 was studied in the present paper. The medium composition and culture condition were optimized in order to improve lipase production. The results showed the best optimum conditions were on the following: culture temperature (40°C); initial pH value (8.0); liquid volume (20%); carbon source (olive oil, 2.5%); nitrogen source (peptone, 1.0%); biosurfactant (Tween-80, 0.2%).

Screening and Desilication of a Silicate Bacteria

2011 ◽  
Vol 236-238 ◽  
pp. 253-257
Author(s):  
Xian Zhen Zhang ◽  
De Si Sun ◽  
Hai Lin
Keyword(s):  
Particle Size ◽  
Incubation Time ◽  
Ph Value ◽  
Solution Temperature ◽  
Optimum Conditions ◽  
Initial Ph ◽  
Silicate Bacteria ◽  
Bauxite Ore

The strain Jgj-1 was isolated from Gaoan bauxite ore. The relations of desilication of the strain Jgj-1 and the pH of solution, temperature, shaking speed, incubation time, particle size were investigated. The results shows the optimum conditions of bioleaching are as following: at 28°C, initial pH value is 7.2, particle size 0.056mm, 200rpm shaking speed, incubation 5-7 days.

scholarly journals Biogas Production from Combined Irish Potato and Poultry Wastes: Optimization and Kinetic Studies

2018 ◽  
Vol 7 (3.36) ◽  
pp. 170
Author(s):  
Umar M. Ibrahim ◽  
Saeed I. Ahmed ◽  
Babagana Gutti ◽  
Idris M. Muhammad ◽  
Usman D. Hamza ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Kinetic Parameters ◽  
Ph Value ◽  
Biogas Production ◽  
Irish Potato ◽  
Coefficient Of Determination ◽  
Optimum Conditions ◽  
Biogas Yield ◽  
Potato Waste ◽  
Initial Ph

The combination of Irish potato waste (IPW) and poultry waste (PW) can form a synergy resulting into an effective substrate for a better biogas production due to some materials they contain. In this work, optimization and kinetic study of biogas production from anaerobic digestion of IPW and PW was investigated. Response surface methodology (RSM) was applied to optimize conditions such as initial pH, solids concentrations and waste ratios. The anaerobic digestion of the two wastes was carried out in the mesophilic condition and Box-Behnken design (BBD) was used to develop and analyze a predictive model which describes the biogas yield. The results revealed that there is a good fit between the experimental and the predicted biogas yield as revealed by the coefficient of determination (R2) value of 97.93%. Optimization using quadratic RSM predicts biogas yield of 19.75% at the optimal conditions of initial pH value 7.28, solids concentration (w/v) 9.85% and waste ratio (IPW:PW) 45:55%. The reaction was observed to have followed a first order kinetics having R2 and relative squared error (RSE) values of 90.61 and 9.63% respectively. Kinetic parameters, such as rate constant and half-life of the biogas yield were evaluated at optimum conditions to be 0.0392 day-1 and 17.68 days respectively. The optimum conditions and kinetic parameters generated from this research can be used to design real bio-digesters, monitor substrate concentrations, simulate biochemical processes and predict performance of bio-digesters using IPW and PW as substrate.  

Optimization of Nitrite Reductase Production Conditions in Lactobacillus plantarum from Salted Fish

2013 ◽  
Vol 781-784 ◽  
pp. 1595-1598 ◽  
Author(s):  
Ya Nan Wang ◽  
Yan Yan Wu ◽  
Xian Qing Yang ◽  
Lai Hao Li ◽  
Bo Qi
Keyword(s):  
Enzyme Activity ◽  
Lactobacillus Plantarum ◽  
Nitrite Reductase ◽  
Ph Value ◽  
Evaluation Index ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Fish Products ◽  
Salted Fish ◽  
Production Conditions

The conditions for nitrite reductase (NiRs) produced by Lactobacillus plantarum from salted fish were optimized. Enzyme activity of nitrite reductase was used as evaluation index. The results showed that the pH value had greatest influence on enzyme activity. The optimum conditions of producing nitrite reductase were inoculate quantity 4 %, pH 6.3, culture temperature 35 °C, incubation time 48 h. The enzyme activity of nitrite reductase reached to 89.672 U/mL, which increased by 71 % after optimization. This work provided the foundation for the research of enzymatic properties of nitrite reductase. This may be useful to the production of salted fish products.

Optimization of Liquid Fermentation for Mycelial Growth and Extracellular Polysaccharide Production by Poria cosos

2012 ◽  
Vol 554-556 ◽  
pp. 962-968
Author(s):  
Shuang Niu ◽  
Li Min Hao ◽  
Shu Xin Zhao ◽  
Fei Fei Wang ◽  
Chun Hui Du
Keyword(s):  
Mycelial Growth ◽  
Ph Value ◽  
Extracellular Polysaccharide ◽  
Liquid Volume ◽  
Growth Line ◽  
Flask Culture ◽  
Culture Experiment ◽  
Fermentation Conditions ◽  
Exopolysaccharide Production ◽  
Initial Ph

This paper is an attempt to study the growth line of poria cocos through flask culture experiment, and leads to a discussion of different fermentation conditions. The optimum media for mycelial growth were determined as (g/l): glucose 60, peptone 10, KNO310, KH2PO43, and MgSO41; while for exopolysaccharide was (g/l), glucose 40, peptone 5, KNO35, KH2PO40.5, MgSO41. The optimum initial pH value of medium was 5.5, inoculation, 10% (v/v) and different liquid volume had minimal influence on the mycelial growth and exopolysaccharide production.

Optimum Conditions of Ag/BiVO4 Photocatalytic Performance on Degradation of Paracetamol by Response Surface Methodology

2014 ◽  
Vol 1073-1076 ◽  
pp. 336-339
Author(s):  
Tian Qi Li ◽  
Hui Wang ◽  
Ya Qi Zhu ◽  
Zhao Yong Bian
Keyword(s):  
Response Surface Methodology ◽  
Visible Light ◽  
Response Surface ◽  
Visible Light Irradiation ◽  
Ph Value ◽  
Light Irradiation ◽  
Catalyst Loading ◽  
Optimum Conditions ◽  
Initial Ph ◽  
Ag Catalyst

Response surface methodology was applied to investigate the optimum degradation conditions of paracetamol using Ag/BiVO4 photocatalysts under the visible light irradiation. Experimental results show that the optimum degradation conditions were: catalyst dosage quantity was 80 mg, Ag-catalyst loading was 5%, and the initial pH value of the solution was 6, respectively. Under this condition, the degradation efficiency of paracetamol was 77.9% within 5 h under the visible light irradiation.

A study on the degradation of direct pink by the low-frequency ultrasonic irradiation

2010 ◽  
Vol 62 (4) ◽  
pp. 829-836 ◽  
Author(s):  
Huaili Zheng ◽  
Guocheng Zhu ◽  
Qiang He ◽  
Peng Hu ◽  
Shijun Jiao ◽  
...  
Keyword(s):  
Ph Value ◽  
Low Frequency ◽  
Alkaline Condition ◽  
Inorganic Salts ◽  
Ultrasonic Power ◽  
Optimum Conditions ◽  
Initial Ph ◽  
H2o2 Oxidation ◽  
Ultrasonic Degradation ◽  
Degradation Degree

The ultrasonic degradation of direct pink was investigated in this study. Parameters affecting ultrasonic degradation degree such as ultrasonic power, pH, bubbling gas and the presence of inorganic salts, were examined. The results showed that the addition of inorganic salts (NaCl, CuSO4) facilitated the degradation of direct pink while the addition of K2CO3 inhibited it. The degradation degree was enhanced significantly in the presence of saturated gases as listed here in decreasing order of effectiveness: argon > air > oxygen > nitrogen. The degradation degree of direct pink was largely influenced by pH value and boosted by acidic condition. The optimum degree could be achieved when pH value was 3.0 or when the sound power was 150 W. However, the degradability decreased notably in alkaline condition. Also, ultrasound/H2O2 technology was used, and the results showed that ultrasound/H2O2 has a better effect on the degradation than ultrasound alone or with H2O2 oxidation. After 120 minutes, the degradation degree could reach 78.0% under the optimum conditions, when the ultrasonic power was 150 W, 50 μL H2O2, CuSO4 and argon atmosphere being added and the initial pH value of the model dye was 3.0.

Optimization of Burkholderia sp. ZYB002 lipase production for pitch control in thermomechanical pulping (TMP) processes

Holzforschung ◽  
2012 ◽  
Vol 66 (3) ◽  
Author(s):  
Zhengyu Shu ◽  
Jiguang Wu ◽  
De Chen ◽  
Lanxing Cheng ◽  
Yi Zheng ◽  
...  
Keyword(s):  
Tween 80 ◽  
Pinus Massoniana ◽  
Fermentation Medium ◽  
Lipase Production ◽  
Thermomechanical Pulp ◽  
Pitch Control ◽  
Thermomechanical Pulping ◽  
Initial Ph ◽  
Inoculum Volume ◽  
Optimized Conditions

AbstractProduction and process optimization of a thermostable lipase fromBurkholderiasp. ZYB002 and the application potential of this lipase for pitch control of thermomechanical pulp (TMP) has been investigated. The optimized physical and nutritional factors ofBurkholderiasp. ZYB002 were found to be (all units in%, w v-1): 0.5 peptone, 0.025 urea, 0.5 glycerol, 0.1 NaHCO3, 0.2 K2HPO4, 1.5 Tween 80, 1.1 olive oil, four inoculum volume, and initial pH 8.5. The lipase production process was carried out at 30°C at 250 rpm for 36 h in 250 ml Erlenmeyer flasks containing 35 ml fermentation medium and five glass beads. Under the optimized conditions, lipase activity reached 216.5 U ml-1. In a TMP ofPinus massoniana, 39% of triglycerides were hydrolyzed by the lipase under optimized conditions [pulp concentration 6% (w v-1), lipase dose 20 U g-1based on dry TMP, pulp initial pH 7.5, and 65°C for 30 min]. The lipase in question displayed a great potential for pitch control of TMP.

scholarly journals The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

2017 ◽  
Vol 4 (10) ◽  
pp. 171012 ◽  
Author(s):  
Miao Ye ◽  
Linghong Sun ◽  
Ru Yang ◽  
Zaigui Wang ◽  
KeZong Qi
Keyword(s):  
Incubation Time ◽  
Bacillus Amyloliquefaciens ◽  
Egg Production ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Liquid Volume ◽  
Hatching Rate ◽  
Culture Temperature ◽  
Egg Weight ◽  
Initial Ph

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p  < 0.05), and the average egg weight was significantly increased in experiment 2 ( p  < 0.05).

scholarly journals New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Mohamed A. Mohamed ◽  
Hassan M. Awad
Keyword(s):  
Large Scale ◽  
Linseed Oil ◽  
Tween 80 ◽  
Fold Increase ◽  
Lipase Production ◽  
Medium Composition ◽  
Scale Production ◽  
Wide Ph Range ◽  
Wadi El Natrun ◽  
Ph Range

Abstract Background Bioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications. Halo-alkaline marshes in Wadi El Natrun in Egypt are some of the most stable ecological systems in the world, and because of the double extremities of alkalinity and salinity, they harbor individual microbes capable of adapting stress conditions. Results Eight strains were recovered from the coastline soil of Al-Beida Lake in Wadi El Natrun and have been tested for lipase production. Among the eight isolates, the strain SBLWN_MH2 was the most active producer of lipase (7.5 U/ml). The crude SBLWN_MH2 lipase showed activity over a wide pH range (3.5 to 13) with an optimum pH at 10.5, and it was able to show more than 75% of its highest activity at pH elevated up to 13. The identification using phenotypic and genotypic methods strongly indicated that the strain SBLWN_MH2 belonged to the genus Streptomyces with a similarity of 99%. Thus, it has been given the suggested name Streptomyces sp. SBLWN_MH2 (MG593538). SBLWN_MH2 produced extracellular lipase in modified starch casein medium supplemented with different oils or Tween-80, and the potential production rate has been attained in the case of linseed oil after 3 days. Further experiments have been carried out to optimize medium composition through Box-Behnken design and response surface methodology, and it was possible to achieve more than 3.5-fold increase in lipase production. Conclusions The present study indicates that Streptomyces sp. SBLWN_MH2 is a potential lipase producer and could be fruitfully employed in the large-scale production of highly alkaline lipase.

scholarly journals Preparation and Photocatalytic Properties of a Bagasse Cellulose-Supported Nano-TiO2 Photocatalytic-Coupled Microbial Carrier

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1645 ◽  
Author(s):  
Jianhua Xiong ◽  
Yinna Liang ◽  
Hao Cheng ◽  
Shuocheng Guo ◽  
Chunlin Jiao ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Mass Fraction ◽  
Photocatalytic Performance ◽  
Ph Value ◽  
Optimum Conditions ◽  
Nano Tio2 ◽  
X Ray ◽  
Preparation Conditions ◽  
Initial Ph ◽  
Composite Carrier

Intimate coupling of photocatalysis and biodegradation (ICPB) has shown promise in removing unwanted organic compounds from water. In this study, bagasse cellulose titanium dioxide composite carrier (SBC-TiO2) was prepared by low-temperature foaming methods. The optimum preparation conditions, material characterization and photocatalytic performance of the composite carrier were then explored. By conducting a single factor test, we found that bagasse cellulose with a mass fraction of 4%, a polyvinyl alcohol solution (PVA) with a mass fraction of 5% and 20 g of a pore-forming agent were optimum conditions for the composite carrier. Under these conditions, good wet density, porosity, water absorption and retention could be realized. Scanning electron microscopy (SEM) results showed that the composite carrier exhibited good biologic adhesion. X-ray spectroscopy (EDS) results confirmed the successful incorporation of nano-TiO2 dioxide into the composite carrier. When the mass concentration of methylene blue (MB) was 10 mg L−1 at 200 mL, 2 g of the composite carrier was added and the initial pH value of the reaction was maintained at 6, the catalytic effect was best under these conditions and the degradation rate reached 78.91% after 6 h. The method of preparing the composite carrier can aid in the degradation of hard-to-degrade organic compounds via ICPB. These results provide a solid platform for technical research and development in the field of wastewater treatment.

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