Effect of Inulin DP on Various Properties of Sugar-Free Dark Chocolates Containing Lactobacillus paracasei and Lactobacillus acidophilus

2017 ◽  
Vol 13 (9) ◽  
Author(s):  
Nevzat Konar ◽  
İbrahim Palabiyik ◽  
Omer Said Toker ◽  
Derya Genc Polat ◽  
Sinem Sener ◽  
...  
Keyword(s):  
Lactobacillus Acidophilus ◽  
Narrow Range ◽  
Quality Parameters ◽  
Lactobacillus Paracasei ◽  
Dark Chocolate ◽  
Rheological Characteristics ◽  
Polymerization Degree ◽  
Melting Profile ◽  
Selection Of ◽  
Control Samples

AbstractIn the present study, the effect of different polymerization degree of inulin on the quality parameters of conventional and sugar-free dark chocolate products as well as on the vitality of probiotics used in the chocolates were investigated. To produce sugar-free dark chocolates prepared with the addition of maltitol,Lactobacillus paracasei/L. acidophilusand inulin with DP higher than 23 and lower than 10 were used as probiotic and prebiotic agents, respectively. After 90 day of storage, more than 6 log cfu/25 g probiotics were observed in the chocolates produced by supplementing of 9 log cfu/25 g probiotics. Considering the probiotic level for both conventional and sugar-free dark chocolate, the highest probiotic level was observed in the samples prepared fromL. acidophilusand inulin with DP<10. In addition, inulin with DP<10 was found as more suitable in terms of rheological characteristics of the samples. Other quality parameters (texture, colour, melting profile and water activity) changed in narrow range which was allowable when compared with the control samples. Consequently, sugar-free chocolate containingL. paracaseiandL. acidophiluscould be produced which might satisfy the expectations of the consumers and appropriate to industrial production similar to conventional chocolate. The findings of the present study highlighted the importance of the selection of the most suitable prebiotic/probiotic combination for maximizing probiotic level in the chocolate products.

scholarly journals Tracing total and dissolved material in a western Canadian basin using quality control samples to guide the selection of fingerprinting parameters for modelling

CATENA ◽  
2021 ◽  
Vol 200 ◽  
pp. 105095
Author(s):  
J.P. Laceby ◽  
P.V.G. Batista ◽  
N. Taube ◽  
M.K. Kruk ◽  
C. Chung ◽  
...  
Keyword(s):  
Quality Control ◽  
Canadian Basin ◽  
Selection Of ◽  
Control Samples

Cam_Gate System in Product Quality Control, Optimal Selection of Inspecting-Sorting Automatic Machines

2013 ◽  
Vol 198 ◽  
pp. 445-450
Author(s):  
Krzysztof Łasiński
Keyword(s):  
Quality Control ◽  
Product Quality ◽  
Ccd Camera ◽  
Quality Parameters ◽  
System Structure ◽  
Optimal Selection ◽  
Product Quality Control ◽  
Factors Affecting ◽  
Dimensional Parameters ◽  
Selection Of

The paper addresses the possibilities of applying the cam_gate system to product quality control. Particular attention is paid to the concept of the test stand simulating the operation of the product quality control automatic machine with the use of the CCD camera. Dimensional parameters of rollers were measured by means of a micrometer screw and the CCD camera at the simulation stand in the cam_gate system and the results of measurements were compared. The paper presents the cost system structure of an enterprises and factors affecting the selection of inspecting-sorting automatic machines in the aspect of the prescribed quality parameters which condition the optimal selection.

Selection of Lactobacillus acidophilus strains for use in ?acidophilus products?

1987 ◽  
Vol 53 (4) ◽  
pp. 215-231 ◽  
Author(s):  
M. C. Johnson ◽  
Bibek Ray ◽  
Tarun Bhowmik
Keyword(s):  
Lactobacillus Acidophilus ◽  
Selection Of

scholarly journals Assessment of diversity by using morphological, biochemical and molecular approaches of selected basmati rice (Oryza sativa L.) varieties

2016 ◽  
Vol 8 (1) ◽  
pp. 69-76 ◽  
Author(s):  
A. Mishra ◽  
Pradeep Kumar ◽  
R. S. Sengar ◽  
P. Kumar ◽  
R. Singh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Molecular Analysis ◽  
Oryza Sativa L ◽  
Quality Parameters ◽  
Basmati Rice ◽  
Molecular Approaches ◽  
Gel Consistency ◽  
Rice Genotypes ◽  
Selection Of

The study investigates the genetic diversity among the Basmati rice genotypes. Selected nine Basmati rice genotypes were studied for twelve morphological traits, biochemical parameters and for molecular analysis with 11 SSR markers. Pusa Sugandha 5 and Basmati 370, showed strong aroma while other varieties showed medium aroma. Alkali spreading value were intermediate in Basmati 386, Vallabh Basmati 22 and Vallabh Basmati 24 while other varieties showed high values. Pusa Basmati 1 and Basmati 386 showed soft category of gel consistency while in rest varieties it was under medium category. Amylose percentage in grains were ranged from 18.02% (Taraori basmati) to 22.0% (Basmati370). Molecular analysis with 11 SSR markers showed 125 allels with an average number of allels 11.36 per locus. All the markers showed specific type of banding pattern along with 82 polymorphic allels in different genotypes. This study focuses on application of statistical methods and techniques in analysis of genetic diversity of the agronomic data, biochemical aspects related to quality parameters and at the molecular level using SSR markers for clustering procedure making dendrogram that helps the more accurate selection of the superior basmati genotypes for the further studies of the breeders and researchers.

scholarly journals Grain Quality and Multivariate Analysis of Various Quality Parameters in Rice Genotypes

2019 ◽  
Vol 39 (04) ◽  
Author(s):  
Kasturi Majumder ◽  
Disharee Nath ◽  
Rambilash Mallick ◽  
Tapash Dasgupta
Keyword(s):  
Significant Variation ◽  
Grain Quality ◽  
Quality Parameters ◽  
Consumer Acceptance ◽  
High Yield ◽  
Rice Breeding ◽  
Quality Traits ◽  
Rice Varieties ◽  
Rice Genotypes ◽  
Selection Of

Thirty-six rice genotypes were evaluated for thirteen different quality parameters along with yield/plant to assess genetic estimates of the traits and the extent of genetic diversity among the varieties. Analysis of variance was conducted to determine GCV, PCV, heritability and GA of the genotypes with respect to all characters. Significant variation was observed in many traits among the genotypes offering scope for selection. Correlation analysis determined the nature of relationship among these characters. UPGMA studies revealed six major clusters and cluster I and II were the largest with maximum number of genotypes. The study identified that the varieties namely, Black Gora, Kalinga-2, Dudheswar, ARC 10086, IR-36, IR-64 and Nipponbare possessed good quality traits and high yield performance. The current study indicated that developing rice varieties for consumer acceptance with good grain quality traits along with high yield will be very useful in rice breeding and in selection of parents for hybridization to combine both high yield and improved quality traits.

scholarly journals DETERMINATION OF FEED QUALITY PARAMETERS OF LEAVES AND STEMS OF DIFFERENT ALFALFA GENOTYPES

2021 ◽  
Vol 10 (20) ◽  
pp. 118-121
Author(s):  
Onur Okumus ◽  
Beyza Ciftci ◽  
Sati Uzun ◽  
Mahmut Kaplan
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Quality Parameters ◽  
Neutral Detergent Fiber ◽  
Crude Protein Content ◽  
Protein Ratio ◽  
Feed Quality ◽  
High Effect ◽  
Selection Of

This research was carried out to determine feed quality parameters of leaves and stems of different alfalfa genotypes. A total of 12 alfalfa genotypes was used as the plant material for the study. Alfalfa plants were harvested at the flowering stage. The plants were dried at 70°C and grinded in a hand-mill with 1 mm sieve for chemical analysis. In leaf, the acid detergent fiber (ADF) content of genotypes varied between 21.62 - 27.40%, the neutral detergent fiber (NDF) content between 33.98 - 39.95%, and crude protein content between 24.68 - 28.45%, whereas in stem ADF content of genotypes varied between 50.99 - 57.72%, NDF content between 66.96 - 76.79 %, crude protein content between 8.46 - 11.00%. The RFV ranged from 159.18 to 197.27% and 53.22 to 67.06% for leaf and stem respectively. The leaf to stem ratio of genotypes ranged from 0.72 to 1.60. Considering current results from different genotypes, genotype has a high effect on feed quality. In addition, the rate of leaf to stems is also effective on feed quality. While the protein ratio of the leaves was higher than the stems, the ADF and NDF contents were found to be lower than the stems. So, in the selection of alfalfa genotypes, attention should be paid to the leaf to stem ratio as well as the yield.

scholarly journals A portable CRISPR-Cas9N system for flexible genome engineering in Lactobacillus acidophilus, Lactobacillus gasseri and Lactobacillus paracasei.

2021 ◽  
Author(s):  
Yong Jun Goh ◽  
Rodolphe Barrangou
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genome Editing ◽  
Lactobacillus Acidophilus ◽  
Genome Engineering ◽  
Gene Deletion ◽  
Lactobacillus Paracasei ◽  
Universal System ◽  
Lactobacillus Gasseri ◽  
Health Promoting

Diverse Lactobacillus strains are widely used as probiotic cultures in the dairy and dietary supplements industries, and specific strains such as Lactobacillus acidophilus NCFM have been engineered for the development of biotherapeutics. To expand the Lactobacillus manipulation toolbox with enhanced efficiency and ease, we present here a CRISPR-SpyCas9D10A nickase (Cas9N)-based system for programmable engineering of L. acidophilus NCFM, a model probiotic bacterium. Successful single-plasmid delivery system was achieved with the engineered pLbCas9N vector harboring cas9N under the regulation of a Lactobacillus promoter and a cloning region for customized sgRNA and editing template. The functionality of the pLbCas9N system was validated in NCFM with targeted chromosomal deletions ranging between 300 bp and 1.9 kb at various loci (rafE, lacS and ltaS), yielding 35-100% mutant recovery rates. Genome analysis of the mutants confirmed precision and specificity of the pLbCas9N system. To showcase the versatility of this system, we also inserted a mCherry fluorescent protein gene downstream of the pgm gene to create a polycistronic transcript. The pLbCas9N system was further deployed in other species to generate concurrent single base substitution and gene deletion in Lactobacillus gasseri ATCC 33323, and an in-frame gene deletion in Lactobacillus paracasei Lpc-37, highlighting the portability of the system in phylogenetically distant Lactobacillus species, where its targeting activity was not interfered by endogenous CRISPR-Cas systems. Collectively, these editing outcomes illustrate the robustness and versatility of the pLbCas9N system for genome manipulations in diverse lactobacilli, and open new avenues for the engineering of health-promoting lactic acid bacteria. Importance This work describes the development of a broad-host range CRISPR-based editing system for genome manipulations in three Lactobacillus species, which belong to lactic acid bacteria (LAB) commonly known for their long history of use in food fermentations and as indigenous members of healthy microbiota, and their emerging roles in human and animal commercial health-promoting applications.  We exploited the established CRISPR-SpyCas9 nickase for flexible and precise genome editing applications in Lactobacillus acidophilus, and further demonstrated the efficacy of this universal system in two distantly related Lactobacillus species.  This versatile Cas9-based system facilitates genome engineering compared to conventional gene replacement systems, and represents a valuable gene editing modality in species that do not possess native CRISPR-Cas systems.  Overall, this portable tool contributes to expanding the genome editing toolbox of LAB for studying their health-promoting mechanisms and engineering of these beneficial microbes as next-generation vaccines and designer probiotics.

scholarly journals Korelasi Pertumbuhan Dan Kandungan Karaginan Kappaphycus alvarezii Dan Eucheuma Spinosum Dengan Jarak Penempatan Rakit Apung Yang Berbeda Di Desa Grujugan Kecamatan Gapura Kabupaten Sumenep [Correlation Of Growth And Carragenan Content Of Kappaphycus alvarezii And Eucheuma Spinosum By Different Distance Placement Of Floating Rafts At The Grujugan Village, Sub-District Gapura, Residence Of Sumenep]

2019 ◽  
Vol 5 (2) ◽  
pp. 217
Author(s):  
Lina Wafia Asmi, Sri Subekti, Moch.Amin Alamsjah
Keyword(s):  
Growth Rate ◽  
Block Design ◽  
Kappaphycus Alvarezii ◽  
Quality Parameters ◽  
Good Growth ◽  
Randomized Block Design ◽  
Final Harvest ◽  
Selection Of ◽  
Temperature Water ◽  
Floating Rafts

Abstract The selection of right location is an important succeed factor of the seaweed cultivation to make seaweed a good growth. Seaweed Kappaphycus alvarezii and Eucheuma spinosum contain carrageenan which has been widely used in several industries. The aim of the research is to determine the correlation of growth and carrageenan content K. alvarezii and E. spinosum by different distance placement of floating rafts. Research method using experimental method and experimental design using randomized block design, followed by Duncan's test or Mann Whitney U test and furthermore linear correlation to determine the correlation between growth and carrageenan content. The treatment using different cultivation locations. On K. alvarezii with a distance of 100 m from the beach (RK1), a distance of 400 m from the beach (RK2), and a distance of 700 m from the beach (RK3). E. spinosum at a distance of 100 m from the beach (RE1), a distance of 400 m from the beach (RE2), and the distance of 700 m from the beach (RE3). Each treatment was repeated 9 times. The main parameters observed in this study is the growth rate of once every 10 days, and the content of carrageenan obtained from the extraction of the final harvest. Supporting water quality parameters include: temperature, water transparency, current velocity, substance nutrients (nitrate and phosphate), salinity, and pH. The results showed seaweed K. alvarezii and E. spinosum cultivated at a distance at 400 m from beach has the highest growth rate and yield best carrageenan content. From the results of the calculation of growth and carrageenan content K. alvarezii and E. spinosum with distance floating rafts of different placements had a very strong correlation

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