scholarly journals The effects of freezing on long-term storage of canine erythrocytes

2016 ◽  
Vol 19 (2) ◽  
pp. 401-406 ◽  
Author(s):  
D. Aktaran Bala ◽  
M. Özcan
Keyword(s):  
Frozen Storage ◽  
Biochemical Properties ◽  
Quality Parameters ◽  
Consecutive Series ◽  
Time Points ◽  
Long Term Storage ◽  
Negative Effect ◽  
Natural Outcome ◽  
2,3 Dpg

Abstract Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at −80°C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na+) and potassium (K+) levels, residual glycerol concentrations and hemograms were evaluated and compared. Sterility tests were performed on all samples for bacterial contamination. A statistically significant decrease was noted in potassium levels, which was the natural outcome of deglycerolization process. No significant change was observed in terms of other parameters due based on different time points. In conclusion, long-term frozen storage had no negative effect on the quality parameters of canine erythrocytes.

scholarly journals Freezing and storage of leukodepleted erythrocyte suspensions

2017 ◽  
Vol 61 (No. 8) ◽  
pp. 443-448 ◽  
Author(s):  
DA Bala ◽  
E. Eraslan ◽  
I. Akyazi ◽  
EE Ekiz ◽  
M. Ozcan ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Bacterial Contamination ◽  
Frozen Storage ◽  
Consecutive Series ◽  
Leukocyte Depletion ◽  
Negative Effect ◽  
And Storage

Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log<sub>10</sub>). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at –80 °C for 4- and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36–38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na<sup>+</sup>) and potassium (K<sup>+</sup>) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction.

Considerations of the technological quality assessment of sugarbeet

2011 ◽  
pp. 85-89 ◽  
Author(s):  
Giorgio Pezzi
Keyword(s):  
Quality Parameters ◽  
Northern Italy ◽  
Glucose Content ◽  
Long Term Storage ◽  
Interesting Correlation ◽  
Technological Quality ◽  
Thick Juice

No real improvement in the technological quality of beet has been recorded over the last 15 years in Northern Italy. Among the possible explanations for the quality stagnation is that the traditional formulae cannot correctly differentiate between sugarbeet varieties which produce thick juice of very high purity. This seems to be connected with the role of potassium. The use of a standard purification procedure gives reliable and accurate data which is immediately comparable with the factory data. Research projects on medium/long term storage are currently being performed by Co.Pro.B., Italy, in cooperation with Syngenta and Beta. Up to now the results have shown that storage of sugarbeet in autumn time in northern Italy is possible provided that suitable varieties and proper handling of the roots are employed. Results obtained in the storage trials are reported. Correlations have been found between quality parameters (purity, color and lime salts) of the purified juice with the glucose content of the raw juice. An interesting correlation is reported between purified juice purity and raw juice purity.

Evaluation of long-term frozen storage of plasma for measurement of high-density lipoprotein and its subfractions by precipitation

1990 ◽  
Vol 36 (5) ◽  
pp. 783-788 ◽  
Author(s):  
M N Nanjee ◽  
N E Miller
Keyword(s):  
High Density Lipoprotein ◽  
Liquid Nitrogen ◽  
Frozen Storage ◽  
Density Lipoprotein ◽  
High Density ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Different Temperatures ◽  
Edta Plasma

Abstract The concentration of high-density lipoprotein cholesterol (HDL-C) in plasma is now established as an independent risk factor for coronary heart disease, but more data are needed on the relative risk-predictive powers of different HDL subclasses. For epidemiologic and clinical purposes, isolation of HDL from other lipoproteins and separation of its two major subclasses, HDL2 and HDL3, are performed most conveniently by precipitation. Although storage of plasma is commonly necessary, little information is available on the long-term stability of HDL subclasses at different temperatures. Therefore, we quantified HDL-C, HDL2-C, and HDL3-C by dual precipitation with heparin-MnCl2/15-kDa dextran sulfate (H-M/DS) in samples of EDTA-plasma from 93 healthy subjects, after storage for one to 433 days at -20 degrees C, at -70 degrees C, or in liquid nitrogen (-196 degrees C). Fourteen samples (15%) were stored for a year or longer. At -20 degrees C, HDL-C decreased by 4.8% per year and HDL3-C decreased by 6.9% per year (P = 0.002 for both variables) relative to results obtained with samples stored in liquid nitrogen; total cholesterol, HDL2-C, and triglyceride did not change significantly at this temperature. When stored at -70 degrees C, none of the lipids showed any change relative to results obtained with liquid nitrogen. Thus, long-term storage of EDTA-plasma at -20 degrees C is unsuitable for subsequent quantification of HDL-C and its subclasses by H-M/DS dual precipitation. Storage at -70 degrees C is preferable, and is as reliable as storage in liquid nitrogen.

scholarly journals The Technological Investigations Of Manufacturing Of Energy Chips

2015 ◽  
Vol 1 ◽  
pp. 220
Author(s):  
Ziedonis Miklašēvičs
Keyword(s):  
Moisture Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Heating Value ◽  
Long Term Storage ◽  
Lower Heating Value ◽  
Term Storage ◽  
Lower Heating

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.

Relationship between Acoustic Firmness and Magness Taylor Firmness in Royal Gala and Golden Smoothee Apples

2009 ◽  
Vol 15 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. Molina-Delgado ◽  
S. Alegre ◽  
J. Puy ◽  
I. Recasens
Keyword(s):  
Quality Parameters ◽  
Acoustic Measurements ◽  
Good Tool ◽  
Malus Domestica Borkh ◽  
Long Term Storage ◽  
Acoustic Methods ◽  
The Relationship ◽  
And Storage ◽  
Term Storage

This study aimed to evaluate apple firmness measured using both the penetrometer and acoustic methods. The methodologies were applied to Royal Gaya and Golden Smoothee apples (Malus domestica. Borkh) harvested from 12 different orchards in Catalonia (Spain), on six different dates, and over three seasons. The relationship between firmness and physicochemical quality parameters was analyzed at harvest and post-harvest. The results obtained showed a noticeable correlation between Magness Taylor firmness and acoustic measurements in Royal Gala, but no correlation was found for Golden Smoothee. Fruit variety and storage had a clear influence on correlations between the two measurements. Measurements of Magnes Taylor and acoustic firmness seemed to correspond to different physical properties. Acoustic measurements seemed to be a good tool for evaluating changes in tissue firmness during long-term storage, especially for Golden Smoothee apples. The maturity of the fruit seemed to play a more important role in the measurement of Magnes Taylor firmness than in the measurement of acoustic firmness for the Royal Gaya variety.

Patterns of lichen photosynthesis and respiration following prolonged frozen storage

1978 ◽  
Vol 56 (17) ◽  
pp. 2119-2123 ◽  
Author(s):  
Douglas W. Larson
Keyword(s):  
Light Intensity ◽  
Low Temperatures ◽  
Physiological Activity ◽  
Frozen Storage ◽  
Experimental Systems ◽  
Long Term Storage ◽  
Photosynthesis And Respiration ◽  
Term Storage ◽  
Respiratory Responses

The effect of prolonged frozen storage on patterns of photosynthesis and respiration in the lichen Alectoria ochroleuca (Hoffm.) Massal. has been examined. The results indicate that this plant not only survives long-term exposure to low temperatures but also that its basic photosynthetic and respiratory responses to temperature, light intensity, and thallus moisture content are altered very little by long-term storage at −60 °C. This maintenance not only of absolute viability but also of the more subtle patterns of physiological activity suggests that such storage may be used to hold lichen material for use in multivariate experimental systems which require replicates having identical field pretreatment.

The Conceptual Solutions Concerning Decomissioning and Dismantling of Russian Civil Nuclear Powered Ships

2013 ◽  
Author(s):  
Konstantin N. Kulikov ◽  
Rinat A. Nizamutdinov ◽  
Andrey N. Abramov
Keyword(s):  
Economic Assessment ◽  
North West ◽  
Long Term Storage ◽  
Murmansk Region ◽  
Negative Effect ◽  
Research Procedure ◽  
State Corporation ◽  
Radiation Contamination ◽  
Selection Of

From 1959 up to 1991 nine civil nuclear powered ships were built in Russia: eight ice-breakers and one lash lighter carrier (cargo ship). At the present time three of them were taking out of service: ice-breaker “Lenin” is decommissioned as a museum and is set for storage in the port of Murmansk, nuclear ice-breakers “Arktika” and “Sibir” are berthing. The ice-breakers carrying radwastes appear to be a possible source of radiation contamination of Murmansk region and Kola Bay because the ship long-term storage afloat has the negative effect on hull’s structures. As the result of this under the auspices of the Federal Targeted Program “Nuclear and Radiation Safety of Russia for 2008 and the period until 2015” the conception and projects of decommissioning of nuclear-powered ships are developed by the State corporation Rosatom with the involvement of companies of United Shipbuilding Corporation. In developing the principal provisions of conception of decommissioning and dismantling of ice-breakers the technical and economic assessment of dismantling options in shiprepairing enterprises of North-West of Russia was performed. The paper contains description of options, research procedure, analysis of options of decommissioning and dismantling of nuclear ice-breakers, taking into account the principle of optimization of potential radioactive effect to personnel, human population and environment. The report’s conclusions contain the recommendations for selection of option for development of nuclear ice-breaker decommissioning and dismantling projects.

scholarly journals Effects of dynamic controlled atmosphere by respiratory quotient on some quality parameters and volatile profile of ‘Royal Gala’ apple after long-term storage

2017 ◽  
Vol 215 ◽  
pp. 483-492 ◽  
Author(s):  
Vanderlei Both ◽  
Fabio Rodrigo Thewes ◽  
Auri Brackmann ◽  
Rogerio de Oliveira Anese ◽  
Daniele de Freitas Ferreira ◽  
...  
Keyword(s):  
Respiratory Quotient ◽  
Quality Parameters ◽  
Controlled Atmosphere ◽  
Volatile Profile ◽  
Long Term Storage ◽  
Term Storage

scholarly journals The Effect of Different Freezing Rates and Long-Term Storage Temperatures on the Stability of Sliced Peaches

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Paul Dawson ◽  
Wesam Al-Jeddawi ◽  
James Rieck
Keyword(s):  
Weight Loss ◽  
Ascorbic Acid ◽  
Prunus Persica ◽  
Frozen Storage ◽  
Critical Factor ◽  
Freezing Rate ◽  
Long Term Storage ◽  
Different Temperatures ◽  
Surface Ice

The purpose of this research was to determine if freezing rates and holding temperatures influence peach quality during short- and long-term frozen storage. Fresh peaches (Prunus persica) were purchased locally, sliced, dipped in 2% ascorbic acid then drained, and packaged. The study was divided into two experiments, one to determine the effect of the freezing rate on peach quality and the second to determine the effect of frozen holding temperatures on peach quality. For the freezing rate experiment, freshly packaged peaches were placed in freezers at different temperatures (-7°C, -12°C, -18°C, -29°C, and -77°C) then removed for testing when the core temperature of the peaches reached the temperature of all freezer temperatures. The second experiment determined the long-term holding effect on quality using both fresh and prefrozen peaches held at -7°C, -12°C, -18°C, -29°C, and -77°C through 360 days. Quality measurements included freeze thaw and weight loss, lightness, firmness, moisture content, ascorbic acid equivalent antioxidant capacity (AAEAC), hexanal detection using gas chromatography (GC), scanning electron microscopy (SEM), and sensory evaluation. During the freezing phase (experiment 1), peach weight loss and surface ice crystal pore size significantly decreased with increased freezing rates. Peaches held at -77°C and -29°C maintained overall quality to a greater degree than the higher holding temperatures. However, all samples enzymatically browned when thawed; therefore, frozen peaches may best if used in the frozen state or in applications where appearance is a critical factor. In general, fresh and prefrozen peaches were not acceptable by the sensory panelists after 270 days of frozen storage.

Effect of dialysis of dog semen on sperm characteristics and some biochemical components of seminal plasma

2015 ◽  
Vol 18 (2) ◽  
pp. 447-448 ◽  
Author(s):  
R. Strzeżek ◽  
M. Koziorowska-Gilun ◽  
K. Kiełczewski ◽  
W. Kordan
Keyword(s):  
Molecular Weight ◽  
Seminal Plasma ◽  
Room Temperature ◽  
Sperm Quality ◽  
Biochemical Properties ◽  
Quality Parameters ◽  
Sperm Characteristics ◽  
Biochemical Components

AbstractThe aim of this study was to investigate the effect of dog semen dialysis on sperm characteristics and some biochemical components of seminal plasma. Whole ejaculates were dialyzed against Tris-citrate-fructose extender for a 5 h period at room temperature (using semi-permeable cellulose tubing of 12-14 kDa molecular weight cut-off). It has been demonstrated that the long-term dialysis of dog semen causes a significant decrease in sperm quality parameters and disrupts the biochemical properties of seminal plasma. This procedure requires further improvement.

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