scholarly journals Comparison of the effect of hemihydrate calcium sulfate granules and Cerabone on dental socket preservation: An animal experiment

2018 ◽  
Vol 12 (4) ◽  
pp. 238-244
Author(s):  
Naser Sargolzaie ◽  
Mehrnaz Rafiee ◽  
Hamideh Salari Sedigh ◽  
Reza Zare Mahmoudabadi ◽  
Hooman Keshavarz
Keyword(s):  
Connective Tissue ◽  
Calcium Sulfate ◽  
Inflammatory Cells ◽  
Histological Evaluation ◽  
Sign Test ◽  
Rank Test ◽  
Fibrous Connective Tissue ◽  
Socket Preservation ◽  
Tissue Proportion

Background. Early bone loss due to tooth extraction can be significantly reduced by socket preservation. The aim of this study was to compare the in vivo effects of hemihydrate calcium sulfate granules (an alloplastic material) and Cerabone (a bovine-derived xenograft) on socket preservation in dogs. Methods. Six male Mongrel dogs were randomly divided into 2 groups (n=3) for sacrificing and histological evaluation 4 and 8 weeks after a surgery. The second and third premolars on both sides of the lower jaw were extracted surgically. The sockets on one side were filled with Cerabone, and with calcium sulfate on the opposite side. In the slides, the ratio of the area of newly formed bone to the area of the entire cavity, and the ratio of the area of fibrous connective tissue to the area of the entire cavity were measured. The presence of inflammation was also examined. Wilcoxon signed-rank test, Sign test and McNemar test were used for statistical analyses (ɑ=0.05). Results. The means of new bone proportion were 11% and 8% for Cerabone and calcium sulfate, respectively (P=0.58). The means of connective tissue proportion were 29% and 33% for Cerabone and calcium sulfate, respectively (P=0.72). No inflammatory cells were observed in the Cerabone group, although 50% of the samples in the calcium sulfate group showed inflammation (P=0.50). Conclusion. The effects of calcium sulfate and Cerabone on socket preservation in dogs on bone formation, fibrous connective tissue and inflammation levels were not significantly different at 4- and 8-week postoperative intervals.

scholarly journals Co-Overexpression of TWIST1-CSF1 Is a Common Event in Metastatic Oral Cancer and Drives Biologically Aggressive Phenotype

Cancers ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 153
Author(s):  
Sabrina Daniela da Silva ◽  
Fabio Albuquerque Marchi ◽  
Jie Su ◽  
Long Yang ◽  
Ludmila Valverde ◽  
...  
Keyword(s):  
Epithelial Mesenchymal Transition ◽  
Inflammatory Cells ◽  
Rank Test ◽  
Mesenchymal Transition ◽  
Genome Wide ◽  
A Genome ◽  
Enhanced Expression ◽  
Advanced Stages

Invasive oral squamous cell carcinoma (OSCC) is often ulcerated and heavily infiltrated by pro-inflammatory cells. We conducted a genome-wide profiling of tissues from OSCC patients (early versus advanced stages) with 10 years follow-up. Co-amplification and co-overexpression of TWIST1, a transcriptional activator of epithelial-mesenchymal-transition (EMT), and colony-stimulating factor-1 (CSF1), a major chemotactic agent for tumor-associated macrophages (TAMs), were observed in metastatic OSCC cases. The overexpression of these markers strongly predicted poor patient survival (log-rank test, p = 0.0035 and p = 0.0219). Protein analysis confirmed the enhanced expression of TWIST1 and CSF1 in metastatic tissues. In preclinical models using OSCC cell lines, macrophages, and an in vivo matrigel plug assay, we demonstrated that TWIST1 gene overexpression induces the activation of CSF1 while TWIST1 gene silencing down-regulates CSF1 preventing OSCC invasion. Furthermore, excessive macrophage activation and polarization was observed in co-culture system involving OSCC cells overexpressing TWIST1. In summary, this study provides insight into the cooperation between TWIST1 transcription factor and CSF1 to promote OSCC invasiveness and opens up the potential therapeutic utility of currently developed antibodies and small molecules targeting cancer-associated macrophages.

scholarly journals Optimized Chitosan/Anion Polyelectrolyte Complex Based Inserts for Vaginal Delivery of Fluconazole: In Vitro/In Vivo Evaluation

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 227 ◽  
Author(s):  
Bayan Darwesh ◽  
Hibah Aldawsari ◽  
Shaimaa Badr-Eldin
Keyword(s):  
Vaginal Delivery ◽  
Polyelectrolyte Complex ◽  
Inflammatory Cells ◽  
Antifungal Drugs ◽  
Vaginal Candidiasis ◽  
Histological Evaluation ◽  
Anionic Polymer ◽  
In Vivo Evaluation

(1) Background: Fluconazole, used orally for vaginal candidiasis, has reported gastrointestinal side effects. Therefore, researchers directed towards the drug vaginal delivery. However, vaginal delivery is limited by poor retention and leakage. Thus, this work aimed at exploring chitosan/anion polyelectrolyte complex (PEC) for the formulation of fluconazole vaginal inserts with controlled release and appreciable mucoadhesion. (2) Methods: PECs were prepared and assessed for interactions. Fluconazole PEC based vaginal inserts were prepared by lyophilization using mannitol. 3151 factorial design was applied to investigate the effect of the anion type and Chitosan/anion ratio on the inserts mucoadhesion and release properties. The optimized insert [based on 5:5 chitosan: anionic polymer (sodium alginate)] release was modulated by the release retardant; Compritol® 888. The selected formulation was subjected to microbiological and histological evaluation. (3) Results: Fluconazole inserts showed satisfactory drug content, acceptable friability percentages and highest swelling indices at six hours. Statistical analysis showed significant effect of the studied factors on detachment force and release properties. Microbiological assays revealed significantly higher antifungal activity of inserts compared to fluconazole solution. Reduced inflammatory cells were confirmed by histological evaluation. (4) Conclusion: CH/Alg based vaginal insert could be a promising platform for vaginal delivery of antifungal drugs used for vaginal candidiasis treatment.

Experimental induced wound cicatrisation after highly diluted products treatment

2021 ◽  
Vol 11 (40) ◽  
pp. 211-212
Author(s):  
Fernando Fortunato Jeronimo ◽  
Jenifer Pendiuk Gonçalves ◽  
Katia Fialho Do Nascimento ◽  
Simone Martins De Oliveira ◽  
Carolina Camargo De Oliveira ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Cells ◽  
Control Group ◽  
Type I ◽  
Chelidonium Majus ◽  
Conium Maculatum ◽  
The Matrix ◽  
Aconitum Napellus

Introduction: Skin is an attractive target to study extracellular matrix, due to abundance in Connective tissue. In cases of injuries the first step is an inflammatory reaction and subsequent the healing that involves several changes in the matrix. These changes are fundamental to inflammatory cells activities allowing healing. Highly diluted products were shown to facilitate inflammatory mediators and to activate immune cells in vivo and in vitro, thus it can be effective to wound healing. Aims: This study aims to evaluate highly diluted products effects on inflammation and cicatrization process. Methodology: Three compounds (M8 (Aconitum napellus 20dH, Arsenicum album 18dH, Asa foetida 20dH, Calcarea carbonica 16dH, Conium maculatum 17dH, Ipecacuanha 13dH, Phosphorus 20dH, Rhus toxicodendron 17dH, Silicea 20dH, Sulphur 24dH, Thuja occidentalis 19dH), M1 (Chelidonium majus 20dH, Cinnamon 20dH, Echinaceae purpurea 20dH, Gelsemium sempervirens 20dH plus all M8 compounds) and Curcuma cH30 – simple product), were manipulated as a gel and applied on mice dorsal flank after incision and suture (approximately 1 cm and three points), for 3 consecutive days. After the treatments the scars were evaluated macroscopically, the animals were killed, the skin samples collected, fixed and processed for Hematoxilin-Eosin (HE) and Masson Tricromic (to observe the collagen fibers type I). The slices were analyzed and images collected by a light microscope Olympus BX51 with camera attached Olympus DP72. Results: It was observed a higher and faster rate of tissue epithelization in the treated groups after three days of gel-product application. This could be observed in lower rates in the control group (no treatment) - Figure 1 and 2). Regeneration and organization of connective tissue were proportional to epithelization the treated groups. We also observed evidences of changes in amount of neutrophils and fibroblasts, resulting in changes in the healing period. Analyses for these confirmations are in progress.

scholarly journals Biological behavior of magnesium-substituted hydroxyapatite during bone repair

2020 ◽  
Author(s):  
G. G. Santos ◽  
V. L. C. Nunes ◽  
S. M. O. C. Marinho ◽  
S. R. A. Santos ◽  
A. M. Rossi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Defect ◽  
Bone Repair ◽  
Bone Mineralization ◽  
Inflammatory Cells ◽  
Biological Behavior ◽  
Osteogenic Potential ◽  
Fibrous Connective Tissue ◽  
Clinical Interest ◽  
Histological Results

Abstract The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI - bone defect filled with HA microspheres; GII - bone defect filled with HA microspheres replaced with Mg; GIII - empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Histopathological Studies on Dermal Fibrous Connective Tissue of Erythema ab Igne.

1993 ◽  
Vol 55 (1) ◽  
pp. 55-60
Author(s):  
Toshihide AKASAKA ◽  
Yuko IMAMURA ◽  
Yasuki MORI ◽  
Satoshi MAYAMA ◽  
Saiichi KON
Keyword(s):  
Connective Tissue ◽  
Fibrous Connective Tissue ◽  
Erythema Ab Igne ◽  
Histopathological Studies

Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

2020 ◽  
Vol 18 ◽  
Author(s):  
Zirui Zhang ◽  
Shangcong Han ◽  
Panpan Liu ◽  
Xu Yang ◽  
Jing Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Tissue Injury ◽  
Inflammatory Cells ◽  
Pcr Analysis ◽  
Protective Effects ◽  
Deep Tissue ◽  
Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

scholarly journals Antidiabetic and antiulcerative potential of Garcinia lanceifolia Roxb. bark

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Nilutpal Sharma Bora ◽  
Partha Sarathi Bairy ◽  
Abdus Salam ◽  
Bibhuti Bhusan Kakoti
Keyword(s):  
Body Weight ◽  
Serum Levels ◽  
Northeast India ◽  
Scientific Data ◽  
Antidiabetic Activity ◽  
Histological Evaluation ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Dose Dependent

Abstract Background Garcinia lanceifolia Roxb. has been used by many ethnic communities of Northeast India to mitigate various disorders like dyspepsia, ulcers, diabetes, etc. However, a robust scientific study on its antidiabetic and antiulcer potential is unavailable till date. The aim of this present study is to scientifically validate if the antidiabetic and antiulcer effects reported by the ethnic tribes of Assam has any scientific value or not. The effects were tested in adult Wistar albino rats using approved animal models for preclinical testing of pharmacological activities. Results The hydroalcoholic extract of the bark of Garcinia lanceifolia Roxb. was prepared and its LD50 was calculated. The LD50 was determined to be greater than 5000 mg/kg body weight. The extract at doses of 250 mg/kg body weight and 500 mg/kg body weight was found to exhibit a very potent dose-dependent antidiabetic activity. The results were backed by a battery of test including analysis of serum levels of blood glucose, lipid profiles, in vivo antioxidant enzymes, and histopathological studies. Evidence of dose-dependent antiulcer activity of the extract was backed by robust scientific data. It was found that HAEGL induced a significant dose-dependent increase in the ulcer index in both alcohol-induced and acetic acid-induced ulcer models, which was evident from the macroscopic observation of the inner lining of the gastric mucosa and the histological evaluation of the extracted stomach. Conclusion The results suggested that the bark of Garcinia lanceifolia (Roxb.) has significant antidiabetic and antiulcer potential. Further studies with respect to the development herbal dosage forms and its safety evaluation are required.

scholarly journals MicroRNA-182-5p relieves murine allergic rhinitis via TLR4/NF-κB pathway

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 1202-1212
Author(s):  
Aichun Zhang ◽  
Yangzi Jin
Keyword(s):  
Allergic Rhinitis ◽  
Inflammatory Cells ◽  
Lavage Fluid ◽  
Specific Ige ◽  
Inflammatory Factors ◽  
Pcr Analysis ◽  
Reverse Transcription Pcr ◽  
Pathway Activation ◽  
Nasal Lavage Fluid

AbstractAllergic rhinitis (AR) is one of the most common chronic diseases. This study examined whether microRNA (miR)-182-5p plays a role in AR by regulating toll-like receptor 4 (TLR4). First, data demonstrated that TLR4 was a target of miR-182-5p. Subsequently, AR mouse model was established to explore the role of miR-182-5p and TLR4 in AR in vivo. Initially, quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that miR-182-5p was downregulated, while TLR4 expression was upregulated in AR mice. Then we found that miR-182-5p mimic reduced the frequency of sneezing and nose rubbing of the AR mice. In addition, miR-182-5p mimic significantly increased ovalbumin (OVA)-specific IgE and leukotriene C4 expression levels in nasal lavage fluid (NLF) and serum of AR mice. miR-182-5p mimic decreased the number of inflammatory cells in NLF of AR mice. It also reduced the levels of inflammatory factors in the serum of AR mice, such as interleukin (IL)-4, IL-5, IL-13, IL-17 and tumor necrosis factor (TNF)-α, while increasing the release of IFN-γ and IL-2. Finally, miR-182-5p mimic inhibited NF-κB signaling pathway activation in AR mice. However, all effects of miR-182-5p mimic on AR mice were reversed by TLR4-plasmid. In conclusion, miR-182-5p/TLR4 axis may represent a novel therapeutic target for AR.

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