Direct Interaction of Adenosine with the TRPV1 Channel Protein

Purpose: To determine the mechanism involved in pregabalin-induced alleviation of postherpetic neuralgia in a rat model.Methods: Ninety-sixty healthy Sprague-Dawley (SD) rats were assigned to sham, model andpregabalin groups (32 rats per group). A model of postherpetic neuralgia (PN) was established. The expressions of IL-1β and TNF-α in spinal cord tissue were determined 7 days after administration of treatments. The proportions of fluorescence areas in astrocytes in the dorsal horn, prefrontal lobe and hippocampus, and level of spinal cord TRPV1 channel protein in each group were evaluated.Results: Relative to model rats, IL-1β and TNF-α in spinal cord of pregabalin rats were significantly reduced (p < 0.05). The areas of fluorescence in astrocytes in dorsal horn of spinal cord, prefrontal lobe and hippocampus of model group were significantly increased, relative to sham, but were decreased in rats in pregabalin group (p < 0.05).Conclusion: Pregabalin significantly alleviates postherpetic neuralgia via mechanisms which may be related to the inflammatory response of spinal dorsal horn and downregulation of TRPV1 channel protein expression. This finding may be useful in developing new drugs for alleviating postherpetic neuralgia.

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The investigation of detection and sensing mechanism of spicy substance based on human TRPV1 channel protein-cell membrane biosensor

Biosensors and Bioelectronics ◽

10.1016/j.bios.2020.112779 ◽

2021 ◽

Vol 172 ◽

pp. 112779

Author(s):

Sa Xiao ◽

Panpan Song ◽

Fanjie Bu ◽

Guangchang Pang ◽

Aimin Zhou ◽

...

Keyword(s):

Cell Membrane ◽

Trpv1 Channel ◽

Sensing Mechanism ◽

Channel Protein ◽

Protein Cell

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Nutrient-regulated gene expression in eukaryotes

Biochemical Society Symposium ◽

10.1042/bss0730085 ◽

2006 ◽

Vol 73 ◽

pp. 85-96 ◽

Cited By ~ 8

Author(s):

Richard J. Reece ◽

Laila Beynon ◽

Stacey Holden ◽

Amanda D. Hughes ◽

Karine Rébora ◽

...

Keyword(s):

Gene Expression ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Transcriptional Control ◽

Direct Interaction ◽

Signalling Pathways ◽

A Cell ◽

One Step ◽

Higher Eukaryotes ◽

Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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Demonstration of a direct interaction between a colonic divalent cation receptor and the basolateral Na−H exchanger

Gastroenterology ◽

10.1016/s0016-5085(01)82630-5 ◽

2001 ◽

Vol 120 (5) ◽

pp. A529-A530

Author(s):

P GEIBEL ◽

M OREILLY ◽

H VIEWEGER ◽

K SIEBERT ◽

N OBREIN ◽

...

Keyword(s):

Divalent Cation ◽

Direct Interaction ◽

Cation Receptor

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Detection of both Type 1 and Type 2 Plasminogen Activator Inhibitors in Human Monocytes

Thrombosis and Haemostasis ◽

10.1055/s-0038-1645688 ◽

1990 ◽

Vol 63 (01) ◽

pp. 067-071 ◽

Cited By ~ 10

Author(s):

Joan C Castellote ◽

Enric Grau ◽

Maria A Linde ◽

Nuria Pujol-Moix ◽

Miquel LI Rutllant

Keyword(s):

Molecular Mass ◽

Plasminogen Activator ◽

Human Peripheral Blood ◽

Direct Interaction ◽

Apparent Molecular Weight ◽

Fibrinolytic System ◽

Human Monocytes ◽

Blood Monocytes ◽

Single Chain ◽

Sds Page

SummaryIncreasing evidence suggests the involvement of leukocytes in the fibrinolytic system. Monocytes secrete pro-urokinase (Grau, Thromb Res 1989; 53: 145) and it has been shown that these cells have specific receptors for urokinase and plasminogen (Miles, Thromb Haemostas 1987; 58: 936). The aim of this study was to analyse the presence of plasminogen activator inhibitor(s) in platelet-free suspensions of human peripheral blood monocytes and polymorphonuclear leukocytes (PMN). SDS-PAGE and reverse fibrin autography showed an inhibitory band of 50 kDa in the monocyte extracts (Triton X-100) but not in the PMN extracts. Urokinase (u-PA) was mixed with increasing amounts of monocyte extract for 10 min and the mixtures were added to 125Ifibrin coated wells containing plasminogen. A dose-dependent decrease in the u-PA fibrinolytic activity was observed. The amount of inhibition increased when the monocyte releasates were preincubated with u-PA (40% inhibition after 5 min preincubation and 80% after 15 min), indicating a direct interaction between this activator and an inhibitor(s). After SDS-PAGE of monocyte extracts, immunoblotting and peroxidase staining identified both PAI1 and PAI2, with an apparent molecular weight of 47-50 kDa. Monocyte-associated PAI1 formed complexes with single chain t-PA with a molecular mass 50 kDa higher than the molecular mass of the free PAI1. However, a significant amount of PAI remained unbound to t-PA. This inactive PAI1 could have come from a rapid inactivation of the primary active PAI1. These PAI1 and PAI2 detected in human monocytes may be transcendent in the regulation of the fibrinolytic system.

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Defibrotide Inhibits Platelet Activation by Cathepsin G Released From Stimulated Polymorphonuclear Leukocytes

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648519 ◽

1992 ◽

Vol 67 (06) ◽

pp. 660-664 ◽

Cited By ~ 17

Author(s):

Virgilio Evangelista ◽

Paola Piccardoni ◽

Giovanni de Gaetano ◽

Chiara Cerletti

Keyword(s):

Platelet Activation ◽

Polymorphonuclear Leukocytes ◽

Direct Interaction ◽

Cathepsin G ◽

In Vivo Test ◽

Cell Functions ◽

Test Models ◽

Antithrombotic Effects

SummaryDefibrotide is a polydeoxyribonucleotide with antithrombotic effects in experimental animal models. Most of the actions of this drug have been observed in in vivo test models but no effects have been reported in in vitro systems. In this paper we demonstrate that defibrotide interferes with polymorphonuclear leukocyte-induced human platelet activation in vitro. This effect was not related to any direct interaction with polymorphonuclear leukocytes or platelets, but was due to the inhibition of cathepsin G, the main biochemical mediator of this cell-cell cooperation. Since cathepsin G not only induces platelet activation but also affects some endothelial cell functions, the anticathepsin G activity of defibrotide could help to explain the antithrombotic effect of this drug.

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How Can Farmers Get Access to Customers?

Voprosy Ekonomiki ◽

10.32609/0042-8736-2013-3-138-151 ◽

2013 ◽

pp. 138-151 ◽

Cited By ~ 1

Author(s):

I. Kotliarov

Keyword(s):

Direct Interaction ◽

Channels Of Distribution ◽

State Strategy ◽

Stimulation Of

The paper analyzes the models of direct interaction between producers and customers of farmers’ products. We demonstrate that the most effective ways of distribution of these products are servicization of retail, e-commerce, vending and direct purchase activity of retailers. The most effective state strategy aimed at supporting farmers’ access to end customers should be the stimulation of these channels of distribution development.

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KUALITAS PELAYANAN IZIN MENDIRIKAN BANGUNAN (IMB) RUMAH TINGGAL DI KECAMATAN KRAMAT JATI JAKARTA TIMUR

JURNAL GOVERNANSI ◽

10.30997/jgs.v1i2.285 ◽

2015 ◽

Vol 1 (2) ◽

pp. 101

Author(s):

Della Arumnitas Sudrajat ◽

R. Akhmad Munjin ◽

Irma Purnamasari

Keyword(s):

Descriptive Analysis ◽

Direct Interaction ◽

Sampling Technique ◽

Service Process ◽

Improve Quality ◽

Quality Service ◽

Building Permit ◽

Service Building ◽

Theory Framework

This study aims to determine how service quality of Building Permit in District Kramat JatiResidential, East Jakarta. The population in this research is 48 people in the district who filed a Keramat Jati Residential petition. The method used in this research is descriptive analysis. The sampling technique used in this study is Incidental Sampling, which sampling technique by coincidence, that anyone who by chance met with the researchers can be used as a sample, which is deemed suitable by research. Then the amount used as many as 48 people. Theory framework used in this study is Ratminto and Winarsih 2006. Service is an activity or a description of the activities that take place in the direct interaction between a person with another person or machine physically and provide customer satisfaction. The result is that the services provided by the Kramat Jati’s subdistrict officer for the community applicant Building Permit Residential, located in the Good criteria with interpretation number is 3.52. The conclusion of this study is still a lack of discipline of officers in the service process that makes the event of delay that hinder the implementation of the applicant service Building Permit Residential, the researchers recommend the discipline of officers need to be improved so that every service process can run well and can improve quality of care provided to the public.Keywords : Quality Service, Building Permit, the Applicant Building Permit

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Localized Die Metallization Damage Induced During Laser-Marking of a Semiconductor Package

ISTFA 2007: Conference Proceedings from the 33rd International Symposium for Testing and Failure Analysis ◽

10.31399/asm.cp.istfa2007p0226 ◽

2007 ◽

Author(s):

Paul Eric B. Parañal

Keyword(s):

Laser Beam ◽

Direct Interaction ◽

Silica Spheres ◽

Layer By Layer ◽

Laser Marking ◽

Molding Compound ◽

Molding Material ◽

Die Surface ◽

Passivation Layers ◽

Propagation Medium

Abstract This paper presents a new fail mechanism for laser-marking induced die damage. Discovered during package qualification, silica spheres – commonly used as fillers in the molding material, was shown to act as a propagation medium that promote the direct interaction of the scribing laser beam and the die surface. Critical to the understanding of the fail mechanism is the deprocessing technique devised to allow layer by layer examination of the metallization and passivation layers in an encapsulated silicon die. The technique also made possible the inspection of the molding compound profile directly on top of the affected die area.

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Training of the cardiology service nursing staff in the conditions of medical organization

Medsestra (Nurse) ◽

10.33920/med-05-2007-04 ◽

2020 ◽

pp. 21-25

Author(s):

Svetlana Inozemtseva ◽

Larisa Karaseva

Keyword(s):

Nursing Staff ◽

Direct Interaction ◽

Theoretical Knowledge ◽

Medical Organization ◽

Main Work ◽

Working Situation ◽

Normal Work

Training in the workplace is characterized by direct interaction with normal work in the usual working situation for specialists, which has a number of advantages: it does not require large financial expenses, separation from the main work and departure from the limits of the settlement, allows to quickly implement the obtained theoretical knowledge in the practical activities of nursing specialists.

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