scholarly journals Hydropriming enhances the germination of aged ultra-dry wheat seeds

2020 ◽  
Vol 48 (1) ◽  
pp. 57-63
Author(s):  
Bowen Jiang ◽  
Liyuan Wang ◽  
Chengtang Xu ◽  
Min Yan
Keyword(s):  
Hydrogen Peroxide ◽  
Amylase Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Oxidative Enzymes ◽  
Distilled Water ◽  
Ambient Conditions ◽  
Germination Traits ◽  
Wheat Seeds

Hydropriming is an efficient strategy for reviving aged seeds. In the present study, hydropriming was optimised for vigour enhancement in aged ultra-dry wheat seeds. Wheat seeds (initially dried to 5.0% moisture content) stored at ambient temperature for ten years were humidified over water for seven days, then soaked for 12, 24 and 36 hours in aerated distilled water (seed priming treatment). Primed and unprimed seeds were equilibrated at ambient conditions for 48 hours. Changes in germination traits, activities of anti-oxidative enzymes, hydrogen peroxide (H2O2), malondialdehyde (MDA), α-amylase activity, soluble protein and soluble sugars were determined. Results indicated that hydro priming for 12 hours significantly increased the germination percentage, from 50.7 to 58.6%. The increase in germination was significantly positively correlated with catalase activity (r = 0.969), and significantly negatively correlated with accumulation of H2O2 (r = 1.000).

scholarly journals Role of priming technique in germination parameters of calendula (Calendula officinalis L.) seeds

2016 ◽  
Vol 61 (3) ◽  
pp. 215-226
Author(s):  
Mahnaz Karimi ◽  
Masomeh Varyani
Keyword(s):  
Enzyme Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Distilled Water ◽  
Vigor Index ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Calendula Officinalis L

Seed priming is known for its promoting effects on seed and seedling properties under stress conditions such as salinity, temperature and drought stresses. Poor plant stand establishment is a common problem for marigold production. Therefore, an experiment was conducted to investigate the effect of different seed priming techniques on calendula seed germination and seedling enzyme activity. For this purpose, different seed priming techniques: hydropriming (distilled water, 24 and 72 h), osmopriming (0.02% or 0.04% KNO3 for 24 and 72 h) and hormonal priming (100 or 500 mg L-1 GA3 for 24 and 72 h) were assessed. Seeds without any treatment were considered as control. Compared with control, 100 mg L-1 GA3 and distilled water for 72 h showed the highest promoting effects on germination percentage. The highest vigor index (525.75) was related to hydropriming (for 24 h) treatment. Also, the maximum root length was found with 0.02% KNO3 for 72 h. Catalase significantly increased in the primed seeds. The highest CAT activity and total soluble sugars were found in the seeds primed with 100 mg L-1 GA3 for 72 h. Moreover, the enzyme activity was significantly higher in the distilled water and KNO3 treatments compared with the untreated seeds.

scholarly journals Evaluation of pregerminative treatments in gulupa seeds (P. edulis f. edulis Sims).

2020 ◽  
Vol 42 (3) ◽  
Author(s):  
María Antonia Martínez Camargo ◽  
Ana Cruz Morillo Coronado ◽  
Edilberto Cepeda Mora ◽  
Sandra Yaneth Mesa Fernández
Keyword(s):  
Hydrogen Peroxide ◽  
Statistical Analysis ◽  
Experimental Design ◽  
Germination Percentage ◽  
Economic Importance ◽  
The Other ◽  
Distilled Water ◽  
Passiflora Edulis ◽  
Numerous Species ◽  
Water Imbibition

Abstract The genus Passiflora, commonly known as fruits of passion, includes numerous species, including gulupa (Passiflora edulis f. edulis), of nutracetic and economic importance, both for consumption in nature and for its agro-industrial applicability. This research was developed to evaluate the effects of various pre-germinative treatments on the germination of gulupa seeds from the municipality of Ramiriquí (Boyacá), such as: exposure to hydrogen peroxide (H2O2), hypochlorite of sodium (NaOCl), gibberellic acid (GA3), distilled water imbibition and stratification at 4 °C, in varying concentrations and exposure times (24 and 48 hours), using an experimental design completely random, with three repetitions of 50 seed each one. Statistical analysis showed significant differences between treatments, with H2O2 being 15% the most effective, with a germination percentage (PG) of 88% in exposed seeds for 48 hours. In the presence of NaOCl the percentages were lower 36 - 40%. GA3 at 100 ppm for 24 hours recorded 51% PG, at the other concentrations of this growth regulator and exposure times this value was lower. The use of H2O2 has not been reported as a treatment to induce germination in the genus Passiflora. These results can be applied for the treatment of seeds in plant species can be a viable, effective, economical and easily applicable alternative.

scholarly journals GERMINATION OF THREE WILDFLOWER SPECIES AFTER SEED PRIMING

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1087f-1087
Author(s):  
G. B. McClure ◽  
F. J. Sundstrom ◽  
N. S. Robbins
Keyword(s):  
Environmental Conditions ◽  
Seed Priming ◽  
Germination Percentage ◽  
Optimum Concentration ◽  
Distilled Water ◽  
Land Restoration ◽  
Industrial Sites ◽  
Coreopsis Tinctoria ◽  
Successful Establishment ◽  
Adverse Environmental Conditions

Wildflower species are being used for highway median vegetation, land restoration programs, and revegetation of industrial sites. Rapid, uniform germination is critical to establishment of new stands Seed priming techniques may increase the potential for successful establishment under adverse environmental conditions. The influence of seed priming of Gaillardia puchella, Monarda citriodora, and Coreopsis tinctoria on germination at 10° and 25° C was investigated. Seeds of the three species were placed in five levels of oxygenated KNO3 (3.5, 3.25, 3.0, 2.75, and 2.5%) solutions, or a control of oxygenated distilled water for 144 hr. Germination percentage and rate of germination (MRG) were improved for Gaillardia and Coreopsis at 10° and 25°C. Germination percentage and MRG for Monarda were not effected by priming at 25°C, but both were significantly improved at 10°C, The optimum concentration of KNO3 for greatest rapid germination was dependent on the seed variety, These results suggest that in most cases wildflower germination percentage and rates ware improved by the priming procedure.

scholarly journals Effects of rhizobial nod factors (Lipo chitooligosaccharide) on seedling growth of maize (Zea mays L.) under salt stress

2018 ◽  
Vol 47 (4) ◽  
pp. 831-837
Author(s):  
D. Udhaya Nandhini ◽  
E. Somasundaram
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Reducing Sugars ◽  
Amylase Activity ◽  
Growth Parameters ◽  
Sugar Content ◽  
Seed Priming ◽  
Germination Percentage ◽  
Nod Factors ◽  
Nod Factor

Effects of nod factors (lipo chitooligosaccharide) on seedling growth of maize under salt stress have been studied. The study was framed with seed priming using lipo chitooligosaccharide (LCO) a nod factor @ 4 ml/kg of seeds and without priming on maize seedling growth which was exposed to different levels of salinity (0, 2, 4, 6, 8 and 10 dS/m). Salinity negatively influenced the seedling growth of maize. However nod factor treated maize seedlings had relatively higher germination percentage, root length, shoot length, reducing sugars and amylase activity. Correlation analysis revealed positive relationships between seedling growth parameters. Thus, seed priming with nod factor (4 ml/kg) improved the resistance to salinity at seedling stage. NaCl concentrations restricted amylase enzyme activity and reducing sugar content in the germinating seeds. Priming of nod factor/LCO mitigated the restricted effects of salinity on amylase activity and reducing sugars.

scholarly journals The Effects of Pre-germination Treatments on Seed Germination and Growth of Wild Guavas in the Kingdom of Eswatini, Southern Africa

2020 ◽  
pp. 28-36
Author(s):  
Thulani Sikhondze ◽  
Kwanele A. Nxumalo ◽  
Michael T. Masarirambi ◽  
Paul K. Wahome ◽  
Mathole G. Zwane
Keyword(s):  
Seed Germination ◽  
Sulphuric Acid ◽  
Growth And Development ◽  
Seed Priming ◽  
Psidium Guajava ◽  
Germination Percentage ◽  
Hot Water ◽  
Distilled Water ◽  
Ecological Zones ◽  
Poor Seed

Dormancy is a condition where seeds will not germinate even when the environmental conditions (water, temperature and aeration) are permissive for germination. Wild guavas (Psidium guajava L.) are very popular in all agro-ecological zones of Eswatini. Farmers have shown an interest towards guava cultivation but have to cope with the shortage of quality propagation material. The demand is not fulfilled because of unavailability of superior seedling rootstocks, which might be due to poor seed germination and seedling growth. Nevertheless, it has been reported that guava seeds exhibited seed dormancy, which affects their growth and development. The experiment was carried out to study effects of different pre-germination methods on seed germination of guava. The study was conducted at the University of Eswatini, Luyengo Campus. The objective of the study was to get maximum germination of guava seeds in as short a time as possible. Four methods were used i.e.,  soaking in distilled water for five days at room temperature, soaking in hot water at 80ºC for three minutes, subjecting seeds to heat at 80ºC in oven for six minutes and soaking in 20% dilute sulphuric acid for three minutes. All these treatments significantly decreased days to germination of seeds compared with the control. Among the methods, treatment of guava seeds with 20% dilute sulphuric acid for three minutes was judged best with maximum germination percentage (93.3%); lowest germination mean time was observed in seeds soaked in distilled water (31 days). Highest plant height (44 mm) and highest stem girth of (3.37 mm) were recorded from seeds soaked in sulphuric acid after 150 days of sowing. Seeds without any pre-germination treatment showed poor germination (26.7%). On the basis of the findings, it can be recommended that propagators use sulphuric acid in seed priming for higher germination, growth and development.

scholarly journals PHYSIOLOGICAL AND BIOCHEMICALS CHANGES MODULATED BY SEEDS’ PRIMING OF LENTIL (Lens culinaris L.) UNDER SALT STRESS AT GERMINATION STAGE

2019 ◽  
Vol 18 (5) ◽  
pp. 27-38 ◽  
Author(s):  
Amal Bouallègue ◽  
Fatma Souissi ◽  
Issam Nouairi ◽  
Monia Souibgui ◽  
Zouhaier Abbes ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Salt Stress ◽  
Root Elongation ◽  
Soluble Sugars ◽  
Primary Root ◽  
Seed Priming ◽  
Germination Percentage ◽  
Embryonic Axis ◽  
Sod Activity ◽  
Germination Stage

Seed priming is one of the potential physiological approaches to enhance seed germination under salt stress. The present study examined the role of two seed priming molecules, salicylic acid (SA) and hydrogen peroxide (H2O2), to enhance the salt tolerance in lentil seeds at germination stage. Salt stress caused significant decrease in germination percentage and primary root elongation. This decrease was associated with significant increase in lipid peroxidation and total lipid (TL) contents in embryonic axis. The catalase (CAT), guaiacol peroxydase (GPOX) and superoxide dismutase (SOD) activities remained unchanged or decreased significantly under the influence of salt stress, in both embryonic axis and cotyledons. Starch mobilization was not affected by salt stress. The two priming treatments effectively alleviated the negative effects of salt stress. SA and H2O2 applications after dose optimization resulted in significant enhancement of germination percentage and primary root elongation. No significant changes in starch, soluble sugars contents and SOD activity were detected following SA and H2O2 treatments. Seed priming treatments triggered the activities of GPOX and CAT and caused the reduction of lipid peroxidation especially in embryonic axis. TL content and especially the fatty acid C18:3 increased after SA applications. The better performance under salt stress of primed lentil seeds was associated with lower lipid peroxidation, and activation of enzymatic antioxidative defense system. Obtained results confirm the potential for using SA and H2O2 to improve germination and plant growth under salt stress conditions.

scholarly journals The Effect Halo-And Hydro-Priming on Seed Reserve Utilization and Seed Germination of Wheat Seeds Under Salinity Stress

2014 ◽  
Vol 47 (3) ◽  
pp. 39-45
Author(s):  
S.A. Tabatabaei
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Utilization Efficiency ◽  
Field Crops ◽  
Germination Characteristics ◽  
Percentage Weight ◽  
Wheat Seeds

Abstract Seed germination and seedling growth are critical stages in the life cycle of a plant, especially under adverse abiotic stresses. Seed germination negatively affected by stress conditions. Seed priming techniques have been used to increase germination characteristics and improve germination uniformity in more field crops under stressed conditions. This experimental aimed to evaluate the effect of salinity and halo-priming on seed reserve utilization and seed germination of wheat seeds. For create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, - 12 and -16 bar was used. Seeds of were pretreated with halo-priming at 10 °C for 24 h and water at 10 °C for 24 h. Our results showed that treatment×drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage was obtained from halo priming in control conditions. Thus priming improved study traits in wheat under salinity stress. Priming increased germination percentage and seed reserve utilization as compared to the unprimed seeds. The highest germination percentage and seed reserve utilization were as obtained from halo priming in control conditions.

scholarly journals Comparative Effects of Hydro-, Hormonal-, Osmotic-, and Redox-Priming on Seed Germination of Creeping Bentgrass under Optimal and Suboptimal Temperatures

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1453-1462
Author(s):  
Kun Jia ◽  
Michelle DaCosta ◽  
J. Scott Ebdon
Keyword(s):  
Hydrogen Peroxide ◽  
Seed Germination ◽  
Creeping Bentgrass ◽  
Seed Priming ◽  
Germination Percentage ◽  
Agrostis Stolonifera ◽  
Putting Greens ◽  
Golf Putting ◽  
Early Emergence ◽  
Temperature Enhancement

Reseeding of creeping bentgrass (Agrostis stolonifera L.) under unfavorable temperature (≈10 °C) is a common practice on golf putting greens and fairways. Seed priming to enhance germination and early emergence increases seeding success. Seed priming comparing abscisic acid (ABA), gibberellic acid (GA), glycinebetaine (GB), hydrogen peroxide (H2O2), and polyethylene glycol (PEG) has not been investigated in turfgrass. Our objective was to compare these chemical primers at three concentrations with water- and unprimed-seed at two competing germinating temperatures (10 vs. 25 °C). Two seed lots of ‘T-1’ creeping bentgrass were compared. Curve fitting of daily germination was used to compute days to 50% germination (D50) and maximum germination percentage (Gmax). Cold (10 °C) significantly inhibited emergence (higher D50) more than Gmax. The effects of primers and their rates varied with the seed lot and temperature. Enhancement of seed germination measured as early emergence (lower D50) and/or higher Gmax were only detected at 10 °C. Osmotic primers (GB and PEG) were most effective in promoting germination relative to unprimed seed followed by hormone primers (ABA and GA) with redox primers (H2O2) least effective. Glycinebetaine primed seed was the only primer effective at all concentrations, with the 100 mм concentration the only concentration to enhance germination by increasing both Gmax and early emergence (lower D50) compared with unprimed seed.

scholarly journals GERMINATION OF THREE WILDFLOWER SPECIES AFTER SEED PRIMING

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1087F-1087
Author(s):  
G. B. McClure ◽  
F. J. Sundstrom ◽  
N. S. Robbins
Keyword(s):  
Environmental Conditions ◽  
Seed Priming ◽  
Germination Percentage ◽  
Optimum Concentration ◽  
Distilled Water ◽  
Land Restoration ◽  
Industrial Sites ◽  
Coreopsis Tinctoria ◽  
Successful Establishment ◽  
Adverse Environmental Conditions

Wildflower species are being used for highway median vegetation, land restoration programs, and revegetation of industrial sites. Rapid, uniform germination is critical to establishment of new stands Seed priming techniques may increase the potential for successful establishment under adverse environmental conditions. The influence of seed priming of Gaillardia puchella, Monarda citriodora, and Coreopsis tinctoria on germination at 10° and 25° C was investigated. Seeds of the three species were placed in five levels of oxygenated KNO3 (3.5, 3.25, 3.0, 2.75, and 2.5%) solutions, or a control of oxygenated distilled water for 144 hr. Germination percentage and rate of germination (MRG) were improved for Gaillardia and Coreopsis at 10° and 25°C. Germination percentage and MRG for Monarda were not effected by priming at 25°C, but both were significantly improved at 10°C, The optimum concentration of KNO3 for greatest rapid germination was dependent on the seed variety, These results suggest that in most cases wildflower germination percentage and rates ware improved by the priming procedure.

Peroxidase Localization in Hartmanella Trophozoites

1971 ◽  
Vol 29 ◽  
pp. 346-347 ◽  
Author(s):  
George E. Childs ◽  
Joseph H. Miller
Keyword(s):  
Hydrogen Peroxide ◽  
Ultrastructural Localization ◽  
Pathogenic Strain ◽  
Oxidative Enzymes ◽  
Differential Centrifugation ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
The Subject ◽  
Axenic Cultures

Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa. Cytochemical tests employing diaminobenzidine-tetra HCl (DAB) and hydrogen peroxide were used for the ultrastructural localization of peroxidases of trophozoites of Hartmanella sp. (A-l, Culbertson), a pathogenic strain grown in axenic cultures of trypticase soy broth.

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