scholarly journals Role of priming technique in germination parameters of calendula (Calendula officinalis L.) seeds

2016 ◽  
Vol 61 (3) ◽  
pp. 215-226
Author(s):  
Mahnaz Karimi ◽  
Masomeh Varyani
Keyword(s):  
Enzyme Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Distilled Water ◽  
Vigor Index ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Calendula Officinalis L

Seed priming is known for its promoting effects on seed and seedling properties under stress conditions such as salinity, temperature and drought stresses. Poor plant stand establishment is a common problem for marigold production. Therefore, an experiment was conducted to investigate the effect of different seed priming techniques on calendula seed germination and seedling enzyme activity. For this purpose, different seed priming techniques: hydropriming (distilled water, 24 and 72 h), osmopriming (0.02% or 0.04% KNO3 for 24 and 72 h) and hormonal priming (100 or 500 mg L-1 GA3 for 24 and 72 h) were assessed. Seeds without any treatment were considered as control. Compared with control, 100 mg L-1 GA3 and distilled water for 72 h showed the highest promoting effects on germination percentage. The highest vigor index (525.75) was related to hydropriming (for 24 h) treatment. Also, the maximum root length was found with 0.02% KNO3 for 72 h. Catalase significantly increased in the primed seeds. The highest CAT activity and total soluble sugars were found in the seeds primed with 100 mg L-1 GA3 for 72 h. Moreover, the enzyme activity was significantly higher in the distilled water and KNO3 treatments compared with the untreated seeds.

scholarly journals Improvement of Germination Characteristics and Enhancement of Antioxidant Enzymes Activity of Safflower (Carthamus tinctorius L.) Aged Seeds by Used of Gibberellin

2015 ◽  
Vol 48 (3) ◽  
pp. 33-41
Author(s):  
M. Khajeh ◽  
S.A. Tabatabaei ◽  
O. Ansari ◽  
F. Sharif Zadeh
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Ascorbate Peroxidase ◽  
Seed Priming ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Characteristics ◽  
Normal Seedling

AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.

scholarly journals Hydropriming enhances the germination of aged ultra-dry wheat seeds

2020 ◽  
Vol 48 (1) ◽  
pp. 57-63
Author(s):  
Bowen Jiang ◽  
Liyuan Wang ◽  
Chengtang Xu ◽  
Min Yan
Keyword(s):  
Hydrogen Peroxide ◽  
Amylase Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Oxidative Enzymes ◽  
Distilled Water ◽  
Ambient Conditions ◽  
Germination Traits ◽  
Wheat Seeds

Hydropriming is an efficient strategy for reviving aged seeds. In the present study, hydropriming was optimised for vigour enhancement in aged ultra-dry wheat seeds. Wheat seeds (initially dried to 5.0% moisture content) stored at ambient temperature for ten years were humidified over water for seven days, then soaked for 12, 24 and 36 hours in aerated distilled water (seed priming treatment). Primed and unprimed seeds were equilibrated at ambient conditions for 48 hours. Changes in germination traits, activities of anti-oxidative enzymes, hydrogen peroxide (H2O2), malondialdehyde (MDA), α-amylase activity, soluble protein and soluble sugars were determined. Results indicated that hydro priming for 12 hours significantly increased the germination percentage, from 50.7 to 58.6%. The increase in germination was significantly positively correlated with catalase activity (r = 0.969), and significantly negatively correlated with accumulation of H2O2 (r = 1.000).

EFFECTS OF DIFFERENT CONCENTRATIONS OF TITANIUM OXIDE NANOPARTICLES AND BULK ON GERMINATION CHARACTERISTICS AND ANTIOXIDANT ENZYMES ACTIVITY OF AVENA SATIVA L. SEEDS

2021 ◽  
Vol 24 (1) ◽  
Author(s):  
Moein Iranmanesh ◽  
◽  
Abolfazl Beyraghdar ◽  
Homa Mahmoodzadeh ◽  
◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Titanium Oxide ◽  
Germination Percentage ◽  
Shoot Length ◽  
Guaiacol Peroxidase ◽  
Seedling Vigor ◽  
Titanium Oxide Nanoparticles ◽  
Vigor Index ◽  
Germination Characteristics ◽  
Peroxidase Enzyme

The aim of this study is to compare the effects of different concentrations of nano and bulk titanium oxide on germination characteristics and the activity of antioxidant enzymes in Avena Sativa L. The titanium oxide nanoparticles diameter was determined about 40 nm using AFM, DLS, XRD analysis. In this study, based on a completely randomized design, seeds were placed in Petri dishes containing different concentrations of nano and bulk titanium oxide (50, 150, 250, 350 ppm). Three replications were considered for each concentration and the germination factors and antioxidant enzyme activity in the treated plant were compared with the control. The average data were compared using one-way ANOVA analysis and DUNCAN tests. According to the results, the following parameters of seedling vigor index, root length, dry weight, wet weight, guaiacol peroxidase enzyme, and polyphenol oxidase were statistically significant. The highest germination percentage, relative germination percentage, germination rate, weight germination index and shoot length were observed at 250 ppm bulk titanium oxide. In addition, the highest average germination time, seedling vigor index, optimum seedling index, and root length were observed in 50 ppm bulk titanium oxide treatment and the highest shoot length was observed in 250 ppm nano-titanium oxide treatment. In the enzyme activity, the highest activity of guaiacol peroxidase enzyme was observed in 150 ppm bulk titanium oxide treatment and the highest Polyphenol oxidase enzyme activity was observed in bulk titanium oxide 350 ppm treatment.

scholarly journals Rice (Oryza sativa L.) Seed Priming: It’s Effect on Seed Germination and Seedling Emergence of Spring Varieties in Kapilvastu District, Nepal

2021 ◽  
Vol 8 (2) ◽  
pp. 196-201
Author(s):  
Sabita Ghimire ◽  
Hariom Yadav ◽  
Prabesh Acharya ◽  
Raju Kharel
Keyword(s):  
Vitamin C ◽  
Root Length ◽  
Oryza Sativa L ◽  
Tap Water ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Length ◽  
Superior Performance ◽  
Vigor Index

This study aimed to determine the effect of seed priming (on-farm, hydro, halo, osmo, vitamin c priming, and hardening) on the germination and seedling growth of spring rice varieties: hardinath-3, hardinath-1, and chaite-5 at ambient room temperature. A factorial completely randomized design (CRD) was carried out with two factors: methods of priming and varieties at agriculture knowledge center (AKC), kapilbastu during march 2020. There were, in total, 21 treatment combinations and four replications. Parameters observed were germination percentage, speed of germination, vigor index, root length, shoot length, fresh weight, and dry weight of rice seedlings. Hardinath-3 had superior performance in terms of germination percentage (95.35 %), vigor index (183.86), root length (11.51 cm) and shoot length (7.39 cm) compared to hardinath-1 and chaite-5; the speed of germination, however, was greater in hardinath-1. Seed hardening - alternate soaking (tap water for 24 hours) and drying - induced higher germination percentage (94.91 %), speed of germination (96.00), and vigor index (197.43) than did other priming methods; germination percentage of vitamin c primed seeds (94.75 %), nevertheless, were at statistical par with hardened seeds. Hardened hardinath-3 seeds were better in regards to germination percentage (97.50 %) and root length (12.92 cm), while the speed of germination (97.50) and vigor index (210.50) was greater in hardened hardinath-1. Vitamin c primed seeds of hardinath-3 and hardinath-1 were finer with dry weight, shoot length, and vigor index. In conclusion, farmers' are suggested to hardened seeds of hardinath-3 before sowing to promote uniform germination and growth.

scholarly journals GERMINATION OF THREE WILDFLOWER SPECIES AFTER SEED PRIMING

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1087f-1087
Author(s):  
G. B. McClure ◽  
F. J. Sundstrom ◽  
N. S. Robbins
Keyword(s):  
Environmental Conditions ◽  
Seed Priming ◽  
Germination Percentage ◽  
Optimum Concentration ◽  
Distilled Water ◽  
Land Restoration ◽  
Industrial Sites ◽  
Coreopsis Tinctoria ◽  
Successful Establishment ◽  
Adverse Environmental Conditions

Wildflower species are being used for highway median vegetation, land restoration programs, and revegetation of industrial sites. Rapid, uniform germination is critical to establishment of new stands Seed priming techniques may increase the potential for successful establishment under adverse environmental conditions. The influence of seed priming of Gaillardia puchella, Monarda citriodora, and Coreopsis tinctoria on germination at 10° and 25° C was investigated. Seeds of the three species were placed in five levels of oxygenated KNO3 (3.5, 3.25, 3.0, 2.75, and 2.5%) solutions, or a control of oxygenated distilled water for 144 hr. Germination percentage and rate of germination (MRG) were improved for Gaillardia and Coreopsis at 10° and 25°C. Germination percentage and MRG for Monarda were not effected by priming at 25°C, but both were significantly improved at 10°C, The optimum concentration of KNO3 for greatest rapid germination was dependent on the seed variety, These results suggest that in most cases wildflower germination percentage and rates ware improved by the priming procedure.

scholarly journals The Effect of Salicylic Acid and Polyetylene Glycol on Wheat Germination

2018 ◽  
Vol 75 (1) ◽  
pp. 38
Author(s):  
Marin SOARE ◽  
Paula IANCU ◽  
Elena BONCIU ◽  
Ovidiu PĂNIȚĂ
Keyword(s):  
Salicylic Acid ◽  
Winter Wheat ◽  
Germination Percentage ◽  
Main Role ◽  
Distilled Water ◽  
Water Control ◽  
Wheat Seedlings ◽  
Vigor Index ◽  
Pre Treatment ◽  
Wheat Germination

The present paper analyses the effect of pre-treatment with salicylic acid (SA) on germination and the growth of winter wheat seedlings in water stress conditions induced by polyetylene glycol (PEG). A two-factor experiment was conducted in a completely randomized projection. The first factor (A) included three levels: a1- distilled water - control; a2 - 0.25 mM SA; a3 - 0.75 mM SA and the second factor (B) included three levels: b1- distilled water; b2 - 15% PEG and b3 - 25% PEG. Biological material was represented by a DH winter wheat line. It were made observations on germination percentage (GP), root length (RL), cotyledons length (CL), seedling fresh weight (SFW) and vigor index (VI). After the analysis of the obtained results it can appreciate that the 25% PEG concentration played the main role for the significant decrease of the germination percentage (GP) and vigor index (VI). Also, seeds pre-treatment with 0.75 mM SA could be a way to improve germination characteristics in drought conditions.

scholarly journals Potential Role of Lolium multiflorum Lam. in the Management of Rice Weeds

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 324
Author(s):  
Sara Vitalini ◽  
Francesca Orlando ◽  
Valentina Vaglia ◽  
Stefano Bocchi ◽  
Marcello Iriti
Keyword(s):  
Weed Management ◽  
Oryza Sativa L ◽  
Lolium Multiflorum ◽  
Germination Percentage ◽  
Vigor Index ◽  
Organic Farmers ◽  
Total Polyphenolic Content ◽  
Species Specific

The phytotoxic relationships between crops and weeds can cover a role in weed management, reducing the use of chemical herbicides. Starting from the organic farmers’ experience, the study aimed to define the inhibitory action of Lolium multiflorum Lam., used as a cover crop before rice sowing, against Echinochloa oryzoides (Ard.) Fritsch, one of the main rice weeds. In vitro 7-day assays were carried out in Petri dishes to compare the effect of different L. multiflorum Lam. parts, in the form of aqueous extract or powder, on the seed germination and seedling growth of Oryza sativa L. and E. oryzoides and to verify the hypothesis of a higher susceptibility of the weed. The total polyphenolic content, as the potential source of allelochemicals, in the L. multiflorum parts was measured. The results showed that both species suffer the phytotoxic action of L. multiflorum, but a more marked effect against E. oryzoides was recorded. In according with the polyphenol quantities, stem and inflorescence extracts showed the more significant species-specific inhibition. In all assays, the weed showed a stronger reduction in the root length and seedling vigor index, and, in some cases, also in the germination percentage and shoot length compared to rice.

scholarly journals Evaluation of allelopathic effect of Carthamus Oxyacantha against wheat and maize seed germination

2020 ◽  
Vol 9 (1) ◽  
pp. 814-818
Keyword(s):  
Seed Germination ◽  
Dry Weight ◽  
Germination Percentage ◽  
Allelopathic Effect ◽  
Distilled Water ◽  
Maize Seed ◽  
Fresh Weight ◽  
Vigor Index ◽  
Seedling Length ◽  
Powder Extract

Effect of Carthamusoxyacantha powder aqueous extract on seed germination of Wheat and Maize were studied. Powder extract of 3g, 6g and 9g of concentration were soaked in distilled water for 12 Hrs, 24 Hrs and 36 Hrs of time. The seeds of Wheat and Maize were germinated in Petri dishes. Control was maintained by watering the seeds with distilled water. The highest values for germination percentage, shoot length, root length, fresh weight, dry weight, seedling length, vigor index and seedling weight vigor index were recorded in control of wheat the above parameter decreased with increasing concentration and timing. While in maize highest values for the above parameters were recorded in treatments as compared to control.

scholarly journals Effect of Seed Priming on Improvement of Germination of Vicia Villosa Under Allelopathic Components of Eucalyptus Camaldulensis

2014 ◽  
Vol 66 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Morteza Saberi ◽  
Farajollah Tamian
Keyword(s):  
Seedling Growth ◽  
Eucalyptus Camaldulensis ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Vicia Villosa ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Early Seedling ◽  
Plant Length

Abstract The objective of this study was to evaluate the effectiveness of seed priming in improving seed germination and seedling vigor of Vicia villosa under laboratory conditions. Chemical stimulators included: gibberel-lic acid (125,250 and 500 ppm), salicylic acid (100,200 and 300 mg/lit) and extract of Eucalyptus camaldulen-sis (0, 25, 50, 75 and 100 %). This experiment was carried out as factorial experiment based on a randomized completely design, with four replications. The results showed that Eucalyptus camaldulensis extract had in-hibitive effect on germination and early seedling growth of Vicia villosa. Early seedling growth of Vicia villosa increased by pretreatment of seeds in chemical stimulators so that the highest effect was observed in gibberellic acid (250 ppm). The chemical stimulators don’t have any effect on germination speed. Interaction effects of allelopathic and pretreatment with chemical stimulators were significance on germination percentage, root, shoot and plant length and seed vigor index.

scholarly journals The Effects of Pre-germination Treatments on Seed Germination and Growth of Wild Guavas in the Kingdom of Eswatini, Southern Africa

2020 ◽  
pp. 28-36
Author(s):  
Thulani Sikhondze ◽  
Kwanele A. Nxumalo ◽  
Michael T. Masarirambi ◽  
Paul K. Wahome ◽  
Mathole G. Zwane
Keyword(s):  
Seed Germination ◽  
Sulphuric Acid ◽  
Growth And Development ◽  
Seed Priming ◽  
Psidium Guajava ◽  
Germination Percentage ◽  
Hot Water ◽  
Distilled Water ◽  
Ecological Zones ◽  
Poor Seed

Dormancy is a condition where seeds will not germinate even when the environmental conditions (water, temperature and aeration) are permissive for germination. Wild guavas (Psidium guajava L.) are very popular in all agro-ecological zones of Eswatini. Farmers have shown an interest towards guava cultivation but have to cope with the shortage of quality propagation material. The demand is not fulfilled because of unavailability of superior seedling rootstocks, which might be due to poor seed germination and seedling growth. Nevertheless, it has been reported that guava seeds exhibited seed dormancy, which affects their growth and development. The experiment was carried out to study effects of different pre-germination methods on seed germination of guava. The study was conducted at the University of Eswatini, Luyengo Campus. The objective of the study was to get maximum germination of guava seeds in as short a time as possible. Four methods were used i.e.,  soaking in distilled water for five days at room temperature, soaking in hot water at 80ºC for three minutes, subjecting seeds to heat at 80ºC in oven for six minutes and soaking in 20% dilute sulphuric acid for three minutes. All these treatments significantly decreased days to germination of seeds compared with the control. Among the methods, treatment of guava seeds with 20% dilute sulphuric acid for three minutes was judged best with maximum germination percentage (93.3%); lowest germination mean time was observed in seeds soaked in distilled water (31 days). Highest plant height (44 mm) and highest stem girth of (3.37 mm) were recorded from seeds soaked in sulphuric acid after 150 days of sowing. Seeds without any pre-germination treatment showed poor germination (26.7%). On the basis of the findings, it can be recommended that propagators use sulphuric acid in seed priming for higher germination, growth and development.

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